RLG00000014402

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017240
Physical Location & Seq
Reverse (-)
52729780 .. 52731443
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014402

Sequence Viewer

Length: 738 bp
ATGCTAAAGGGAAACTCCCAAGAAATTAGCCAAAGGGACCAGCGGTGTTCGGTCACGGTGGATGGGAAATGCACCACAGTCGAGCAGGAGAAGTACTTTGACAACGGGCTGCCAACTGGAGTCGGAGGAAGTCGCTGCAGGATGATTTTGGAGAAACTGAAGAAGCTCAAGACCTCTGATGACCACTGTGCTGAAAGGCTGCTCGAACAGATCGAATCTTTGCGCAATGAGTACGAAAATACGCTCGAAAATTCTGAACTTGCAAAACACGAGATTGAGATGGAGCATGAACGTGCATGTGCTCTGGAAAAACGAAAAACAAAGAAAAATAAACGCACTGCGACAACTTTGCTCATTTGCGGCACTGTGGCAGCTACTATGGTTGGTCATCCACACATGGGTGCCGCTGCCGCTATGGTTATTCGTCGACAAGTTTCTAAAGCCTGTAGCTCAAGAACTAAAGAGGAAGAACAACGACATGAAATTGCTCTCAAGGCTCACTACTCAATGGAAGGAAAAATGATTGTTAGCATTAAGGAGCTGGGCAGCATTAAGTTTCTCATTGATAAGATTGTAAATGAGATCGATTCACTCTTGAGAAGTGTAGAGGGGGCAATTGACACTGGAGAAGTAGATTTCGCAATGAAGACTATCAAGAATAAGATGGAAACATTTCGGAAGAAAATTGACGAATTGAATACAGAAGCAGGTAAATCTATAGTCGACCTACATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

246

Amino Acids

27.61

Weight (kDa)

8.1

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 43 - 238 2.2e-18 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 224
Acc36I ACCTGC 1 cut(s) 698
AccB1I GGYRCC 1 cut(s) 401
AccI GTMKAC 2 cut(s) 427, 723
AciI CCGC 4 cut(s) 43, 360, 405, 411
AcsI RAATTY 1 cut(s) 250
AcuI CTGAAG 1 cut(s) 179
AfaI GTAC 2 cut(s) 95, 233
AfiI CCNNNNNNNGG 1 cut(s) 398
AgsI TTSAA 1 cut(s) 697
AleI CACNNNNGTG 1 cut(s) 399
AluBI AGCT 4 cut(s) 166, 374, 450, 541
AluI AGCT 4 cut(s) 166, 374, 450, 541
Alw21I GWGCWC 1 cut(s) 304
ApeKI GCWGC 6 cut(s) 109, 135, 199, 371, 407, 546
ApoI RAATTY 1 cut(s) 250
Asp700I GAANNNNTTC 1 cut(s) 672
AspLEI GCGC 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 37
AvaII GGWCC 1 cut(s) 37
BanI GGYRCC 1 cut(s) 401
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 653
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 6 cut(s) 96, 122, 186, 383, 394, 558
BccI CCATC 3 cut(s) 56, 274, 658
BcgI CGANNNNNNTGC 4 cut(s) 61, 95, 331, 365
BfmI CTRYAG 3 cut(s) 136, 445, 717
BfuAI ACCTGC 1 cut(s) 698
BisI GCNGC 9 cut(s) 110, 136, 200, 361, 372, 405, 408, 411, 547
BlsI GCNGC 9 cut(s) 111, 137, 201, 362, 373, 406, 409, 412, 548
BmcAI AGTACT 1 cut(s) 95
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 2 cut(s) 38, 403
BpiI GAAGAC 1 cut(s) 653
BpmI CTGGAG 2 cut(s) 138, 645
BpuEI CTTGAG 4 cut(s) 152, 436, 476, 616
Bsa29I ATCGAT 1 cut(s) 585
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse1I ACTGG 2 cut(s) 121, 628
Bse3DI GCAATG 2 cut(s) 232, 648
BseCI ATCGAT 1 cut(s) 585
BseGI GGATG 3 cut(s) 67, 147, 388
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMI GCAATG 2 cut(s) 232, 648
BseNI ACTGG 2 cut(s) 121, 628
BseXI GCAGC 6 cut(s) 96, 122, 186, 383, 394, 558
BseYI CCCAGC 1 cut(s) 541
BshNI GGYRCC 1 cut(s) 401
BshVI ATCGAT 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 304
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 2 cut(s) 210, 582
BspACI CCGC 4 cut(s) 43, 360, 405, 411
BspDI ATCGAT 1 cut(s) 585
BspLI GGNNCC 2 cut(s) 38, 403
BspMAI CTGCAG 1 cut(s) 140
BspMI ACCTGC 1 cut(s) 698
BspT107I GGYRCC 1 cut(s) 401
BsrDI GCAATG 2 cut(s) 232, 648
BsrI ACTGG 2 cut(s) 121, 628
BssMI GATC 2 cut(s) 210, 582
BssSI CACGAG 1 cut(s) 269
Bst2BI CACGAG 1 cut(s) 269
Bst4CI ACNGT 4 cut(s) 58, 79, 188, 367
BstF5I GGATG 3 cut(s) 67, 147, 388
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 2 cut(s) 213, 585
BstMBI GATC 2 cut(s) 210, 582
BstMWI GCNNNNNNNGC 2 cut(s) 410, 494
BstNSI RCATGY 1 cut(s) 300
BstSFI CTRYAG 3 cut(s) 136, 445, 717
BstV1I GCAGC 6 cut(s) 96, 122, 186, 383, 394, 558
BstV2I GAAGAC 1 cut(s) 653
Bsu15I ATCGAT 1 cut(s) 585
BsuTUI ATCGAT 1 cut(s) 585
BtsCI GGATG 3 cut(s) 67, 147, 388
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 4 cut(s) 184, 336, 363, 621
BveI ACCTGC 1 cut(s) 698
CfoI GCGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 37
ClaI ATCGAT 1 cut(s) 585
Csp6I GTAC 2 cut(s) 94, 232
CviAII CATG 4 cut(s) 287, 297, 397, 479
CviJI RGCY 9 cut(s) 30, 109, 166, 199, 374, 443, 450, 497, 541
CviKI_1 RGCY 9 cut(s) 30, 109, 166, 199, 374, 443, 450, 497, 541
CviQI GTAC 2 cut(s) 94, 232
DpnI GATC 2 cut(s) 212, 584
DpnII GATC 2 cut(s) 210, 582
Eco47I GGWCC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 179
FaeI CATG 4 cut(s) 290, 300, 400, 482
FaiI YATR 8 cut(s) 288, 298, 380, 398, 416, 480, 719, 732
FaqI GGGAC 1 cut(s) 50
FatI CATG 4 cut(s) 286, 296, 396, 478
FblI GTMKAC 2 cut(s) 427, 723
Fnu4HI GCNGC 9 cut(s) 110, 136, 200, 361, 372, 405, 408, 411, 547
FokI GGATG 3 cut(s) 74, 154, 375
Fsp4HI GCNGC 9 cut(s) 110, 136, 200, 361, 372, 405, 408, 411, 547
FspI TGCGCA 1 cut(s) 224
GlaI GCGC 1 cut(s) 224
GluI GCNGC 9 cut(s) 110, 136, 200, 361, 372, 405, 408, 411, 547
GsaI CCCAGC 1 cut(s) 545
GsuI CTGGAG 2 cut(s) 138, 645
HhaI GCGC 1 cut(s) 225
Hin1II CATG 4 cut(s) 290, 300, 400, 482
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HincII GTYRAC 2 cut(s) 428, 724
HindII GTYRAC 2 cut(s) 428, 724
HinfI GANTC 3 cut(s) 120, 215, 587
Hpy166II GTNNAC 2 cut(s) 428, 724
Hpy188I TCNGA 4 cut(s) 125, 178, 256, 678
Hpy188III TCNNGA 5 cut(s) 169, 305, 453, 595, 655
Hpy8I GTNNAC 2 cut(s) 428, 724
Hpy99I CGWCG 1 cut(s) 429
HpyAV CCTTC 1 cut(s) 506
HpyCH4III ACNGT 4 cut(s) 58, 79, 188, 367
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 4 cut(s) 72, 138, 263, 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 494
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 4 cut(s) 290, 300, 400, 482
HspAI GCGC 1 cut(s) 223
Kzo9I GATC 2 cut(s) 210, 582
LmnI GCTCC 2 cut(s) 283, 538
LpnPI CCDG 9 cut(s) 53, 71, 102, 124, 290, 457, 527, 609, 693
Lsp1109I GCAGC 6 cut(s) 96, 122, 186, 383, 394, 558
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 1 cut(s) 52
MalI GATC 2 cut(s) 212, 584
MboI GATC 2 cut(s) 210, 582
MboII GAAGA 4 cut(s) 172, 479, 658, 691
MfeI CAATTG 1 cut(s) 615
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 7 cut(s) 24, 250, 483, 615, 684, 692, 733
MlyI GAGTC 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 103
MnlI CCTC 4 cut(s) 119, 184, 457, 601
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 3 cut(s) 534, 552, 736
MslI CAYNNNNRTG 2 cut(s) 291, 399
MspA1I CMGCKG 2 cut(s) 43, 407
MunI CAATTG 1 cut(s) 615
MwoI GCNNNNNNNGC 2 cut(s) 410, 494
NdeII GATC 2 cut(s) 210, 582
NlaIII CATG 4 cut(s) 290, 300, 400, 482
NlaIV GGNNCC 2 cut(s) 38, 403
NmuCI GTSAC 1 cut(s) 52
NsbI TGCGCA 1 cut(s) 224
NspI RCATGY 1 cut(s) 300
OliI CACNNNNGTG 1 cut(s) 399
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 2 cut(s) 215, 587
PkrI GCNGC 9 cut(s) 111, 137, 201, 362, 373, 406, 409, 412, 548
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
PspFI CCCAGC 1 cut(s) 541
PspN4I GGNNCC 2 cut(s) 38, 403
PspPI GGNCC 1 cut(s) 37
PstI CTGCAG 1 cut(s) 140
RsaI GTAC 2 cut(s) 95, 233
RsaNI GTAC 2 cut(s) 94, 232
RseI CAYNNNNRTG 2 cut(s) 291, 399
SalI GTCGAC 2 cut(s) 426, 722
SaqAI TTAA 3 cut(s) 534, 552, 736
SatI GCNGC 9 cut(s) 110, 136, 200, 361, 372, 405, 408, 411, 547
Sau3AI GATC 2 cut(s) 210, 582
Sau96I GGNCC 1 cut(s) 37
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 1 cut(s) 129
SduI GDGCHC 1 cut(s) 304
SetI ASST 8 cut(s) 168, 176, 295, 376, 452, 543, 712, 729
SfcI CTRYAG 3 cut(s) 136, 445, 717
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 2 cut(s) 291, 399
SmlI CTYRAG 4 cut(s) 167, 451, 491, 595
SmoI CTYRAG 4 cut(s) 167, 451, 491, 595
Sse9I AATT 7 cut(s) 24, 250, 483, 615, 684, 692, 733
SsiI CCGC 4 cut(s) 43, 360, 405, 411
TaaI ACNGT 4 cut(s) 58, 79, 188, 367
TaiI ACGT 1 cut(s) 295
TaqI TCGA 7 cut(s) 81, 204, 213, 246, 427, 585, 723
TaqII GACCGA 1 cut(s) 40
TasI AATT 7 cut(s) 24, 250, 483, 615, 684, 692, 733
TatI WGTACW 1 cut(s) 93
TauI GCSGC 3 cut(s) 363, 407, 413
TfiI GAWTC 2 cut(s) 215, 587
Tru1I TTAA 3 cut(s) 534, 552, 736
Tru9I TTAA 3 cut(s) 534, 552, 736
TscAI CASTG 4 cut(s) 191, 343, 370, 628
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 6 cut(s) 109, 135, 199, 371, 407, 546
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 3 cut(s) 303, 495, 659
TspRI CASTG 4 cut(s) 191, 343, 370, 628
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 250
XceI RCATGY 1 cut(s) 300
XmiI GTMKAC 2 cut(s) 427, 723
XmnI GAANNNNTTC 1 cut(s) 672
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.