Rh6AG119400

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0017240
Physical Location & Seq
Forward (+)
17921256 .. 17950883
29628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG119400.1

Sequence Viewer

Length: 852 bp
ATGACGGATCTGAGTAAGTTCGTAGAGTCAGCTCGTGAAAATCATCAATTTATTCAATCTGTTGTTCAACAGTACTTTGACAACGGGCTGCCAACTGGAGTCGGAGGAAGTCGCTGCAGGATGATTTTGGAGAAACTGAAGAAGCTCAAGACCTCTGATGACCACTGTGCTGAAAGGCTGCTCGAACAGATCGAATCTTTGCGCAATGAGTACGAAAATACGCTCGAAAATTCTGAACTTGCAAAACACGAGATTGAGATGGAGCATGAACGTGCATGTGCTCTGGAAAAACGAAAAACAAAGAAAAATAAACGCACTGCGACAACTTTGCTCATTTGCGGCACTGTGGCAGCTACTATGGTTGGTCATCCATTCATGGGTGCCGCTGCCGCTATGGTTGTTCGTCGACAAGTTTCTAAAGCCTGTAGCTCAAGAACTAAAGAGGAAGAACAACGACATGAAATTGCTTTCAAGGATCACTACGCAATGGAAGGAAAAATGATTATTAGCATTAAGGAGCTGGGCAGCATTAAGTTTCTCATTGATAAGGTTGTAAATGAGATCGATTCACTCTTGAGAAGTGTAGAGGGAGCAATTAACACTGGAGAAGTAGATTTCGCAATGAAGACTATTAAGAATAAGATGGAAACGTTTCGGAAGCAAATTGACGAATTGAGTAGAGAAGCAGGGATTGCTGATAGGTTTTCCATTCGATCAAGAGCACCTGACAGACTAGGTCTACATGATGATCCCCAAAATAGAGAGATCAATTTCACTCACAAGCTCAAGTTGGAACATTTACGTGACGAAGTGATTTACACTAACATAAAACTTATAACAAGTCGCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

283

Amino Acids

32.42

Weight (kDa)

8.19

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 3 - 235 8.8e-20 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 836
Acc16I TGCGCA 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 380
AccI GTMKAC 2 cut(s) 406, 739
AccII CGCG 1 cut(s) 846
AciI CCGC 3 cut(s) 339, 384, 390
AclI AACGTT 1 cut(s) 650
AclWI GGATC 3 cut(s) 15, 483, 743
AcsI RAATTY 1 cut(s) 229
AcuI CTGAAG 1 cut(s) 158
AfaI GTAC 2 cut(s) 74, 212
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 3 cut(s) 56, 68, 472
AluBI AGCT 6 cut(s) 32, 145, 353, 429, 520, 784
AluI AGCT 6 cut(s) 32, 145, 353, 429, 520, 784
Alw21I GWGCWC 2 cut(s) 283, 724
AlwI GGATC 3 cut(s) 15, 483, 743
ApeKI GCWGC 6 cut(s) 88, 114, 178, 350, 386, 525
ApoI RAATTY 1 cut(s) 229
Asp700I GAANNNNTTC 1 cut(s) 651
AspLEI GCGC 1 cut(s) 204
BanI GGYRCC 1 cut(s) 380
BauI CACGAG 2 cut(s) 33, 248
BbsI GAAGAC 1 cut(s) 632
Bbv12I GWGCWC 2 cut(s) 283, 724
BbvI GCAGC 6 cut(s) 75, 101, 165, 362, 373, 537
BccI CCATC 2 cut(s) 253, 637
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BfaI CTAG 1 cut(s) 734
BfmI CTRYAG 2 cut(s) 115, 424
BisI GCNGC 9 cut(s) 89, 115, 179, 340, 351, 384, 387, 390, 526
BlsI GCNGC 9 cut(s) 90, 116, 180, 341, 352, 385, 388, 391, 527
BmcAI AGTACT 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 382
BpiI GAAGAC 1 cut(s) 632
BpmI CTGGAG 2 cut(s) 117, 624
BpuEI CTTGAG 4 cut(s) 131, 415, 595, 770
Bsa29I ATCGAT 1 cut(s) 564
BsaAI YACGTR 1 cut(s) 803
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 2 cut(s) 100, 607
Bse3DI GCAATG 3 cut(s) 211, 492, 627
BseCI ATCGAT 1 cut(s) 564
BseGI GGATG 2 cut(s) 126, 367
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMI GCAATG 3 cut(s) 211, 492, 627
BseNI ACTGG 2 cut(s) 100, 607
BseXI GCAGC 6 cut(s) 75, 101, 165, 362, 373, 537
BseYI CCCAGC 1 cut(s) 520
Bsh1236I CGCG 1 cut(s) 846
BshNI GGYRCC 1 cut(s) 380
BshVI ATCGAT 1 cut(s) 564
BsiHKAI GWGCWC 2 cut(s) 283, 724
BslI CCNNNNNNNGG 1 cut(s) 377
Bsp1286I GDGCHC 2 cut(s) 283, 724
Bsp143I GATC 7 cut(s) 7, 189, 475, 561, 713, 748, 765
Bsp68I TCGCGA 1 cut(s) 846
BspACI CCGC 3 cut(s) 339, 384, 390
BspDI ATCGAT 1 cut(s) 564
BspFNI CGCG 1 cut(s) 846
BspLI GGNNCC 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 119
BspPI GGATC 3 cut(s) 15, 483, 743
BspT107I GGYRCC 1 cut(s) 380
BsrDI GCAATG 3 cut(s) 211, 492, 627
BsrI ACTGG 2 cut(s) 100, 607
BssMI GATC 7 cut(s) 7, 189, 475, 561, 713, 748, 765
BssSI CACGAG 2 cut(s) 33, 248
Bst2BI CACGAG 2 cut(s) 33, 248
Bst4CI ACNGT 3 cut(s) 72, 167, 346
BstAPI GCANNNNNTGC 1 cut(s) 692
BstBAI YACGTR 1 cut(s) 803
BstDEI CTNAG 1 cut(s) 11
BstF5I GGATG 2 cut(s) 126, 367
BstFNI CGCG 1 cut(s) 846
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 7 cut(s) 10, 192, 478, 564, 716, 751, 768
BstMBI GATC 7 cut(s) 7, 189, 475, 561, 713, 748, 765
BstMWI GCNNNNNNNGC 2 cut(s) 389, 692
BstNSI RCATGY 1 cut(s) 279
BstSFI CTRYAG 2 cut(s) 115, 424
BstUI CGCG 1 cut(s) 846
BstV1I GCAGC 6 cut(s) 75, 101, 165, 362, 373, 537
BstV2I GAAGAC 1 cut(s) 632
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
Bsu15I ATCGAT 1 cut(s) 564
BsuTUI ATCGAT 1 cut(s) 564
BtsCI GGATG 2 cut(s) 126, 367
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 4 cut(s) 163, 315, 342, 600
BtuMI TCGCGA 1 cut(s) 846
CfoI GCGC 1 cut(s) 204
ClaI ATCGAT 1 cut(s) 564
Csp6I GTAC 2 cut(s) 73, 211
CviAII CATG 5 cut(s) 266, 276, 376, 458, 743
CviJI RGCY 9 cut(s) 32, 88, 145, 178, 353, 422, 429, 520, 784
CviKI_1 RGCY 9 cut(s) 32, 88, 145, 178, 353, 422, 429, 520, 784
CviQI GTAC 2 cut(s) 73, 211
DdeI CTNAG 1 cut(s) 11
DpnI GATC 7 cut(s) 9, 191, 477, 563, 715, 750, 767
DpnII GATC 7 cut(s) 7, 189, 475, 561, 713, 748, 765
Eco57I CTGAAG 1 cut(s) 158
FaeI CATG 5 cut(s) 269, 279, 379, 461, 746
FaiI YATR 9 cut(s) 267, 277, 359, 377, 395, 459, 744, 827, 836
FatI CATG 5 cut(s) 265, 275, 375, 457, 742
FblI GTMKAC 2 cut(s) 406, 739
Fnu4HI GCNGC 9 cut(s) 89, 115, 179, 340, 351, 384, 387, 390, 526
FokI GGATG 2 cut(s) 133, 354
Fsp4HI GCNGC 9 cut(s) 89, 115, 179, 340, 351, 384, 387, 390, 526
FspBI CTAG 1 cut(s) 734
FspI TGCGCA 1 cut(s) 203
GlaI GCGC 1 cut(s) 203
GluI GCNGC 9 cut(s) 89, 115, 179, 340, 351, 384, 387, 390, 526
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 2 cut(s) 117, 624
HhaI GCGC 1 cut(s) 204
Hin1II CATG 5 cut(s) 269, 279, 379, 461, 746
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HincII GTYRAC 1 cut(s) 407
HindII GTYRAC 1 cut(s) 407
HinfI GANTC 4 cut(s) 26, 99, 194, 566
Hpy166II GTNNAC 2 cut(s) 407, 740
Hpy188I TCNGA 5 cut(s) 12, 104, 157, 235, 657
Hpy188III TCNNGA 7 cut(s) 35, 148, 284, 432, 574, 717, 845
Hpy8I GTNNAC 2 cut(s) 407, 740
Hpy99I CGWCG 1 cut(s) 408
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 3 cut(s) 72, 167, 346
HpyCH4IV ACGT 3 cut(s) 271, 650, 802
HpyCH4V TGCA 3 cut(s) 117, 242, 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 692
HpyF3I CTNAG 1 cut(s) 11
HpySE526I ACGT 3 cut(s) 271, 650, 802
Hsp92II CATG 5 cut(s) 269, 279, 379, 461, 746
HspAI GCGC 1 cut(s) 202
Kzo9I GATC 7 cut(s) 7, 189, 475, 561, 713, 748, 765
LmnI GCTCC 3 cut(s) 262, 517, 590
LpnPI CCDG 8 cut(s) 81, 103, 269, 436, 506, 588, 672, 738
Lsp1109I GCAGC 6 cut(s) 75, 101, 165, 362, 373, 537
MaeI CTAG 1 cut(s) 734
MaeII ACGT 3 cut(s) 271, 650, 802
MaeIII GTNAC 1 cut(s) 803
MalI GATC 7 cut(s) 9, 191, 477, 563, 715, 750, 767
MboI GATC 7 cut(s) 7, 189, 475, 561, 713, 748, 765
MboII GAAGA 3 cut(s) 151, 458, 637
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 283, 724
MluCI AATT 7 cut(s) 47, 229, 462, 594, 663, 671, 769
MlyI GAGTC 2 cut(s) 35, 108
MmeI TCCRAC 2 cut(s) 82, 771
MnlI CCTC 4 cut(s) 98, 163, 436, 580
MroXI GAANNNNTTC 1 cut(s) 651
MseI TTAA 5 cut(s) 513, 531, 597, 633, 850
MslI CAYNNNNRTG 2 cut(s) 270, 801
MspA1I CMGCKG 1 cut(s) 386
MvnI CGCG 1 cut(s) 846
MwoI GCNNNNNNNGC 2 cut(s) 389, 692
NdeII GATC 7 cut(s) 7, 189, 475, 561, 713, 748, 765
NlaIII CATG 5 cut(s) 269, 279, 379, 461, 746
NlaIV GGNNCC 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 803
NruI TCGCGA 1 cut(s) 846
NsbI TGCGCA 1 cut(s) 203
NspI RCATGY 1 cut(s) 279
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 651
PfeI GAWTC 2 cut(s) 194, 566
PflFI GACNNNGTC 1 cut(s) 735
PkrI GCNGC 9 cut(s) 90, 116, 180, 341, 352, 385, 388, 391, 527
PleI GAGTC 2 cut(s) 34, 107
PpsI GAGTC 2 cut(s) 34, 107
Ppu21I YACGTR 1 cut(s) 803
PsiI TTATAA 1 cut(s) 836
Psp1406I AACGTT 1 cut(s) 650
PspFI CCCAGC 1 cut(s) 520
PspN4I GGNNCC 1 cut(s) 382
PstI CTGCAG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 7
PsyI GACNNNGTC 1 cut(s) 735
RruI TCGCGA 1 cut(s) 846
RsaI GTAC 2 cut(s) 74, 212
RsaNI GTAC 2 cut(s) 73, 211
RseI CAYNNNNRTG 2 cut(s) 270, 801
SalI GTCGAC 1 cut(s) 405
SaqAI TTAA 5 cut(s) 513, 531, 597, 633, 850
SatI GCNGC 9 cut(s) 89, 115, 179, 340, 351, 384, 387, 390, 526
Sau3AI GATC 7 cut(s) 7, 189, 475, 561, 713, 748, 765
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 2 cut(s) 35, 108
SduI GDGCHC 2 cut(s) 283, 724
SfcI CTRYAG 2 cut(s) 115, 424
SmiMI CAYNNNNRTG 2 cut(s) 270, 801
SmlI CTYRAG 4 cut(s) 146, 430, 574, 785
SmoI CTYRAG 4 cut(s) 146, 430, 574, 785
Sse9I AATT 7 cut(s) 47, 229, 462, 594, 663, 671, 769
SsiI CCGC 3 cut(s) 339, 384, 390
SspMI CTAG 1 cut(s) 734
TaaI ACNGT 3 cut(s) 72, 167, 346
TaiI ACGT 3 cut(s) 274, 653, 805
TaqI TCGA 6 cut(s) 183, 192, 225, 406, 564, 712
TasI AATT 7 cut(s) 47, 229, 462, 594, 663, 671, 769
TatI WGTACW 1 cut(s) 72
TauI GCSGC 3 cut(s) 342, 386, 392
TfiI GAWTC 2 cut(s) 194, 566
Tru1I TTAA 5 cut(s) 513, 531, 597, 633, 850
Tru9I TTAA 5 cut(s) 513, 531, 597, 633, 850
TscAI CASTG 4 cut(s) 170, 322, 349, 607
TseFI GTSAC 1 cut(s) 803
TseI GCWGC 6 cut(s) 88, 114, 178, 350, 386, 525
Tsp45I GTSAC 1 cut(s) 803
TspDTI ATGAA 4 cut(s) 282, 364, 474, 638
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 4 cut(s) 170, 322, 349, 607
Tth111I GACNNNGTC 1 cut(s) 735
XapI RAATTY 1 cut(s) 229
XceI RCATGY 1 cut(s) 279
XmiI GTMKAC 2 cut(s) 406, 739
XmnI GAANNNNTTC 1 cut(s) 651
XspI CTAG 1 cut(s) 734
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.