RLG00000014417

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017240
Physical Location & Seq
Reverse (-)
52941486 .. 52946853
5368 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014417

Sequence Viewer

Length: 885 bp
ATGACGGAACTGAGTAAGTTCGTAGAGTCAGCTCGTGAAAATCATAAATTTATTCAATCTGTTGTTCAACAGTACTTTGACAACGGGCTGCCAACTGGAGTCGGAGGAAGTCGCTGCAGGATGATTTTGGAGAAACTGAAGAAGCTCAAGACCTCTGATGACCATTGTGCTGAAAGGCTGCTCGAACAGATCGAATCTTTGCGCAATGAGTACGAAAATACGCTCGAAAATTCTGAACTTGCAAAACACGAGATTGAGATGGAGCATGAACGTGCATGTGCTCTGGAAAAACGAAAAACAAAGAAAAATAAACGCACTGCGAGAACTTTGCTCATTTGCGGCACTGTGGCAGCTACTATGGTTGGTCATCCACACATAGGTGCCGCTGCCGCTATGGTTGTTCGTCGACAAGTTTCTAAAGCCTGTAGCTCAAGAACTAAAGAGGAAGAACAACGACATGAAATTGCTCTCCAGGCTCACTACTCAATGGAAGGAAAAGTGATTGTTAGCATTAAGGAGCTGGGCAGCATTAAGTTTCTCATTGATAAGGTTGTAAATGAGATCGATTCACTCCTGAGAAGTGTAGAGGGTGCAATTAACACTGGAGAAGTAGATTTCGCAATGAAGACTATTAAGAATAAGATGGAAACGTTTCGGAAGCAAATTGACGAATTGAATACAGAAGCAGATTATATTGCGTTGAGGGGAGCGGTCACATTCAAGGAGACGAGCGGTCATAGAGGAAGCAGTCACTGTCATCGAGACCAGCGTCATGGTCATGGATACCGGTGTCCTGTTTATGGAGACCGACGGTCACGGAGGCTAGTGTCATCGTCTTCGAGACTAGCGATCACGGTCAATGAGACTGATGTCACCATCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

295

Amino Acids

33.28

Weight (kDa)

8.99

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 3 - 230 4.8e-21 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 380
AccBSI CCGCTC 2 cut(s) 710, 732
AccI GTMKAC 1 cut(s) 406
AciI CCGC 5 cut(s) 339, 384, 390, 710, 732
AclI AACGTT 1 cut(s) 650
AcsI RAATTY 2 cut(s) 47, 229
AcuI CTGAAG 1 cut(s) 158
AfaI GTAC 2 cut(s) 74, 212
AfiI CCNNNNNNNGG 2 cut(s) 377, 800
AgeI ACCGGT 1 cut(s) 786
AgsI TTSAA 4 cut(s) 56, 68, 676, 721
AhdI GACNNNNNGTC 2 cut(s) 732, 811
AjnI CCWGG 1 cut(s) 471
AleI CACNNNNGTG 1 cut(s) 378
AluBI AGCT 5 cut(s) 32, 145, 353, 429, 520
AluI AGCT 5 cut(s) 32, 145, 353, 429, 520
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 5 cut(s) 719, 756, 798, 835, 857
AlwNI CAGNNNCTG 1 cut(s) 753
ApeKI GCWGC 6 cut(s) 88, 114, 178, 350, 386, 525
ApoI RAATTY 2 cut(s) 47, 229
AsiGI ACCGGT 1 cut(s) 786
Asp700I GAANNNNTTC 1 cut(s) 651
AspLEI GCGC 1 cut(s) 204
AsuHPI GGTGA 1 cut(s) 865
BaeI ACNNNNGTAYC 2 cut(s) 775, 808
BanI GGYRCC 1 cut(s) 380
BauI CACGAG 2 cut(s) 33, 248
BbsI GAAGAC 2 cut(s) 632, 828
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 6 cut(s) 75, 101, 165, 362, 373, 537
BccI CCATC 3 cut(s) 253, 637, 884
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 1 cut(s) 473
BciVI GTATCC 1 cut(s) 776
BcoDI GTCTC 5 cut(s) 719, 756, 798, 835, 857
BfaI CTAG 3 cut(s) 824, 845, 883
BfmI CTRYAG 2 cut(s) 115, 424
BfuI GTATCC 1 cut(s) 776
BisI GCNGC 9 cut(s) 89, 115, 179, 340, 351, 384, 387, 390, 526
BlsI GCNGC 9 cut(s) 90, 116, 180, 341, 352, 385, 388, 391, 527
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 1 cut(s) 473
BmeRI GACNNNNNGTC 2 cut(s) 732, 811
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 473
BoxI GACNNNNGTC 2 cut(s) 768, 869
BpiI GAAGAC 2 cut(s) 632, 828
BpmI CTGGAG 3 cut(s) 117, 455, 624
BpuEI CTTGAG 2 cut(s) 131, 415
Bsa29I ATCGAT 1 cut(s) 564
BsaI GGTCTC 2 cut(s) 756, 798
BsaWI WCCGGW 1 cut(s) 786
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 800
Bse118I RCCGGY 1 cut(s) 786
Bse1I ACTGG 2 cut(s) 100, 607
Bse3DI GCAATG 2 cut(s) 211, 627
BseBI CCWGG 1 cut(s) 473
BseCI ATCGAT 1 cut(s) 564
BseGI GGATG 2 cut(s) 126, 367
BseLI CCNNNNNNNGG 2 cut(s) 377, 800
BseMI GCAATG 2 cut(s) 211, 627
BseMII CTCAG 1 cut(s) 566
BseNI ACTGG 2 cut(s) 100, 607
BseXI GCAGC 6 cut(s) 75, 101, 165, 362, 373, 537
BseYI CCCAGC 1 cut(s) 520
BshNI GGYRCC 1 cut(s) 380
BshTI ACCGGT 1 cut(s) 786
BshVI ATCGAT 1 cut(s) 564
BsiHKAI GWGCWC 1 cut(s) 283
BsiSI CCGG 1 cut(s) 787
BslI CCNNNNNNNGG 2 cut(s) 377, 800
BsmAI GTCTC 5 cut(s) 719, 756, 798, 835, 857
BsmBI CGTCTC 1 cut(s) 719
Bso31I GGTCTC 2 cut(s) 756, 798
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 3 cut(s) 189, 561, 849
BspACI CCGC 5 cut(s) 339, 384, 390, 710, 732
BspCNI CTCAG 1 cut(s) 567
BspDI ATCGAT 1 cut(s) 564
BspLI GGNNCC 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 380
BspTNI GGTCTC 2 cut(s) 756, 798
BsrBI CCGCTC 2 cut(s) 710, 732
BsrDI GCAATG 2 cut(s) 211, 627
BsrFI RCCGGY 1 cut(s) 786
BsrI ACTGG 2 cut(s) 100, 607
BssAI RCCGGY 1 cut(s) 786
BssMI GATC 3 cut(s) 189, 561, 849
BssSI CACGAG 2 cut(s) 33, 248
Bst2BI CACGAG 2 cut(s) 33, 248
Bst2UI CCWGG 1 cut(s) 473
Bst4CI ACNGT 5 cut(s) 72, 346, 755, 813, 856
BstDEI CTNAG 2 cut(s) 11, 575
BstF5I GGATG 2 cut(s) 126, 367
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 3 cut(s) 192, 564, 852
BstMAI GTCTC 5 cut(s) 719, 756, 798, 835, 857
BstMBI GATC 3 cut(s) 189, 561, 849
BstMWI GCNNNNNNNGC 2 cut(s) 389, 473
BstNI CCWGG 1 cut(s) 473
BstNSI RCATGY 1 cut(s) 279
BstPAI GACNNNNGTC 2 cut(s) 768, 869
BstSCI CCNGG 1 cut(s) 471
BstSFI CTRYAG 2 cut(s) 115, 424
BstV1I GCAGC 6 cut(s) 75, 101, 165, 362, 373, 537
BstV2I GAAGAC 2 cut(s) 632, 828
BstXI CCANNNNNNTGG 1 cut(s) 773
Bsu15I ATCGAT 1 cut(s) 564
BsuI GTATCC 1 cut(s) 776
BsuTUI ATCGAT 1 cut(s) 564
BtsCI GGATG 2 cut(s) 126, 367
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 4 cut(s) 315, 342, 600, 751
CaiI CAGNNNCTG 1 cut(s) 753
CfoI GCGC 1 cut(s) 204
Cfr10I RCCGGY 1 cut(s) 786
ClaI ATCGAT 1 cut(s) 564
CseI GACGC 1 cut(s) 758
Csp6I GTAC 2 cut(s) 73, 211
CspAI ACCGGT 1 cut(s) 786
CviAII CATG 5 cut(s) 266, 276, 458, 773, 779
CviQI GTAC 2 cut(s) 73, 211
DdeI CTNAG 2 cut(s) 11, 575
DpnI GATC 3 cut(s) 191, 563, 851
DpnII GATC 3 cut(s) 189, 561, 849
DriI GACNNNNNGTC 2 cut(s) 732, 811
Eam1105I GACNNNNNGTC 2 cut(s) 732, 811
Eco31I GGTCTC 2 cut(s) 756, 798
Eco57I CTGAAG 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 471
Esp3I CGTCTC 1 cut(s) 719
FaeI CATG 5 cut(s) 269, 279, 461, 776, 782
FatI CATG 5 cut(s) 265, 275, 457, 772, 778
FblI GTMKAC 1 cut(s) 406
Fnu4HI GCNGC 9 cut(s) 89, 115, 179, 340, 351, 384, 387, 390, 526
FokI GGATG 2 cut(s) 133, 354
Fsp4HI GCNGC 9 cut(s) 89, 115, 179, 340, 351, 384, 387, 390, 526
FspBI CTAG 3 cut(s) 824, 845, 883
FspI TGCGCA 1 cut(s) 203
GlaI GCGC 1 cut(s) 203
GluI GCNGC 9 cut(s) 89, 115, 179, 340, 351, 384, 387, 390, 526
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 3 cut(s) 117, 455, 624
HapII CCGG 1 cut(s) 787
HgaI GACGC 1 cut(s) 758
HhaI GCGC 1 cut(s) 204
Hin1II CATG 5 cut(s) 269, 279, 461, 776, 782
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HincII GTYRAC 1 cut(s) 407
HindII GTYRAC 1 cut(s) 407
HinfI GANTC 4 cut(s) 26, 99, 194, 566
HpaII CCGG 1 cut(s) 787
HphI GGTGA 1 cut(s) 865
Hpy166II GTNNAC 1 cut(s) 407
Hpy188I TCNGA 4 cut(s) 104, 157, 235, 657
Hpy188III TCNNGA 7 cut(s) 35, 148, 284, 432, 574, 761, 840
Hpy8I GTNNAC 1 cut(s) 407
Hpy99I CGWCG 2 cut(s) 408, 813
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 5 cut(s) 72, 346, 755, 813, 856
HpyCH4IV ACGT 2 cut(s) 271, 650
HpyCH4V TGCA 4 cut(s) 117, 242, 275, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 473
HpyF3I CTNAG 2 cut(s) 11, 575
HpySE526I ACGT 2 cut(s) 271, 650
Hsp92II CATG 5 cut(s) 269, 279, 461, 776, 782
HspAI GCGC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 189, 561, 849
LmnI GCTCC 3 cut(s) 262, 517, 707
Lsp1109I GCAGC 6 cut(s) 75, 101, 165, 362, 373, 537
MaeI CTAG 3 cut(s) 824, 845, 883
MaeII ACGT 2 cut(s) 271, 650
MaeIII GTNAC 4 cut(s) 712, 749, 813, 871
MalI GATC 3 cut(s) 191, 563, 851
MbiI CCGCTC 2 cut(s) 710, 732
MboI GATC 3 cut(s) 189, 561, 849
MboII GAAGA 4 cut(s) 151, 458, 637, 828
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 6 cut(s) 47, 229, 462, 594, 663, 671
MlyI GAGTC 2 cut(s) 35, 108
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 7 cut(s) 98, 163, 436, 580, 696, 734, 813
MroXI GAANNNNTTC 1 cut(s) 651
MseI TTAA 4 cut(s) 513, 531, 597, 633
MslI CAYNNNNRTG 3 cut(s) 270, 378, 777
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 1 cut(s) 787
MspR9I CCNGG 1 cut(s) 473
MvaI CCWGG 1 cut(s) 473
MwoI GCNNNNNNNGC 2 cut(s) 389, 473
NdeII GATC 3 cut(s) 189, 561, 849
NlaIII CATG 5 cut(s) 269, 279, 461, 776, 782
NlaIV GGNNCC 1 cut(s) 382
NmuCI GTSAC 4 cut(s) 712, 749, 813, 871
NsbI TGCGCA 1 cut(s) 203
NspI RCATGY 1 cut(s) 279
OliI CACNNNNGTG 1 cut(s) 378
PcsI WCGNNNNNNNCGW 3 cut(s) 189, 766, 845
PdmI GAANNNNTTC 1 cut(s) 651
PfeI GAWTC 2 cut(s) 194, 566
PinAI ACCGGT 1 cut(s) 786
PkrI GCNGC 9 cut(s) 90, 116, 180, 341, 352, 385, 388, 391, 527
PleI GAGTC 2 cut(s) 34, 107
PpsI GAGTC 2 cut(s) 34, 107
PshAI GACNNNNGTC 2 cut(s) 768, 869
Psp1406I AACGTT 1 cut(s) 650
Psp6I CCWGG 1 cut(s) 471
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 1 cut(s) 471
PspN4I GGNNCC 1 cut(s) 382
PstI CTGCAG 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 753
RsaI GTAC 2 cut(s) 74, 212
RsaNI GTAC 2 cut(s) 73, 211
RseI CAYNNNNRTG 3 cut(s) 270, 378, 777
SalI GTCGAC 1 cut(s) 405
SaqAI TTAA 4 cut(s) 513, 531, 597, 633
SatI GCNGC 9 cut(s) 89, 115, 179, 340, 351, 384, 387, 390, 526
Sau3AI GATC 3 cut(s) 189, 561, 849
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 2 cut(s) 35, 108
ScrFI CCNGG 1 cut(s) 473
SduI GDGCHC 1 cut(s) 283
SfcI CTRYAG 2 cut(s) 115, 424
SmiMI CAYNNNNRTG 3 cut(s) 270, 378, 777
SmlI CTYRAG 2 cut(s) 146, 430
SmoI CTYRAG 2 cut(s) 146, 430
Sse9I AATT 6 cut(s) 47, 229, 462, 594, 663, 671
SsiI CCGC 5 cut(s) 339, 384, 390, 710, 732
SspMI CTAG 3 cut(s) 824, 845, 883
StyD4I CCNGG 1 cut(s) 471
TaaI ACNGT 5 cut(s) 72, 346, 755, 813, 856
TaiI ACGT 2 cut(s) 274, 653
TaqI TCGA 7 cut(s) 183, 192, 225, 406, 564, 760, 839
TaqII GACCGA 1 cut(s) 822
TasI AATT 6 cut(s) 47, 229, 462, 594, 663, 671
TatI WGTACW 1 cut(s) 72
TauI GCSGC 3 cut(s) 342, 386, 392
TfiI GAWTC 2 cut(s) 194, 566
Tru1I TTAA 4 cut(s) 513, 531, 597, 633
Tru9I TTAA 4 cut(s) 513, 531, 597, 633
TscAI CASTG 4 cut(s) 322, 349, 607, 758
TseFI GTSAC 4 cut(s) 712, 749, 813, 871
TseI GCWGC 6 cut(s) 88, 114, 178, 350, 386, 525
Tsp45I GTSAC 4 cut(s) 712, 749, 813, 871
TspDTI ATGAA 3 cut(s) 282, 474, 638
TspGWI ACGGA 2 cut(s) 20, 832
TspRI CASTG 4 cut(s) 322, 349, 607, 758
XapI RAATTY 2 cut(s) 47, 229
XceI RCATGY 1 cut(s) 279
XmiI GTMKAC 1 cut(s) 406
XmnI GAANNNNTTC 1 cut(s) 651
XspI CTAG 3 cut(s) 824, 845, 883
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.