RLG00000015817

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012554
Physical Location & Seq
Forward (+)
1620708 .. 1624713
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015817

Sequence Viewer

Length: 1902 bp
ATGTTCTTCGGCATTAGATCGCCAATCCCGTCGAAACATTTGGTTCTTTTTGTGATCATAACCATCTCCTTCGTCTTTGTTGTTTCAGCTTCTGCAGAAAGCCACCACCTTTCTCACACTATCCGATTGACAGGGGGAGAGCAAATAAATGATGGTGGGTTGATCACATGGGGAAGAAGGAGGTCTCTTCAAGAAAACGAGACTTTGGTACTGGCTAAGGAAAGGACACGGAGGAAGGACCCTCTAAATAGATTTAAGAAGTACACTGGGGGCTGGAATATAACAGAACGGCATTACTGGGCTTCAGTAGGTTTTACCGCGGCTCCTTTCTTTGTCGCTGCAATTTTGTGGTTCGTGATTTGCGGGTTAAGCTTGTGCCTTATCTGTATCTGTTATTGCTGTCGTAAAAAAGAGCCTTATGGCTATTCTAGAACAGCATATTGCCTCTCTCTCGTCACCCTCATTATCTTCACCCTCATGGCAATTGCTGGATGCGTTGTGCTGTACATGGGTCAGGGATTGTTCTACGACAGTACAAGAAAGACATTGGATTACCTTGTGAAGGAGGCAGATGAAACTGCTCAAAACCTCAGGAATGTGTCAGATTCTCTTAGCGCAGCTAAGAAGATCGGACTTGACGCCGTAGTTCCTCCGACAGATATTCAGAATCAAATTGACGAGAGTATGGGAACAATAAAGTATGTTTCCAATTTTCTTTCTGAAACAACTGATAAGACTGCACGGAAGTCAAATCAAGTCTTAACAGACTTGAAGCTGGCGCTCATGGTTTTTTCTGCTGTTATGCTCCTTCTGGTCTTTATTGGATTTGCACTTTCCATGTGCGGGATGCGATTTCTTGTATACTGCTTGGTGATTGTTGGGTGGATTCTTGTTACCTGTACATTCATCTTGTGCGGTACATTTCTCCTCATCCATAATGCGTTTGGAGATGCATGTGTCTCCATGGATGAGTGGATTCAAAAGCACCCCACAGCCCACACTGCTTTGGATGATCTACTTCCATGTGTGGACAAAAATGTAAGCCAGAGCATGTTGGAACGGACCAAGATGGCAACCTACTTTGCTGTTCAAGCGGTTAATCGTGTCATCACCGATGGTGCAAATCTTGATCCTTCTGACAGTCCATTAAATTACAATCAGTCTGGCCCTTTATTACCACCAGTCTGCAACCCCTTCAATGAGGATAAAACTGATCGGAAATGTGTCCCTGGTGAAGTGGAATTGCAACAAGCACAAGAGGTATGGCAGAAATACGTATGTAAGGTTTCTAGTCCAACTGGCCAGTGTGTATCACCAGGGCGGCTGACTCCTACCTACTTCGATCAAATGTCAGCTTTTGTAAACGTGAGCAACGCACTGTATCGGTACAGTCCATTCTTTGCTGATCTTGCAGACTGCACATTTGCTAAGGATACATTCTCGGACATAAGTAAACAGCATTGTCCCGGTCTGCGACTACATAGTAAATGGATCTACATTGGCTTGTTAATGGTAGCCTTAGCTGTAATGTTGTCTATGATCTTCTGGGTGATTTACTGTCGGGAACGAAGACATCGTTTGTATACTAAAAAATGTGACAGTTTACACTATGACAGTTTAAACTATGGCAGTGCAAACTATGGCAGTGCAAATCATGGCAGTCGAAACTTTGGCAGTGCAAATCATGGCAGCAGAAACTTTGGCAGTGGAAATCATGGCAGCAGAAACTTTGGCAGTGCAAATCATGGCAGTAGAAACTTTGGCAGTGGAAATCATGGCAGTAGAAACTTCGGCAGCGCAAATCATGGCAGTAGAAACTTCGGCAGCGCAAATCATGGCAGTAGAAACTTTGGCAGTGGAAATCATGGCAGTAGAAACTTTGGCAGTGCAAATACTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

634

Amino Acids

70.24

Weight (kDa)

8.93

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pentapeptide_2 PF01469 540 - 574 7.1e-09 Pentapeptide repeats (8 copies)
Pentapeptide_2 PF01469 570 - 609 1.8e-09 Pentapeptide repeats (8 copies)
Pentapeptide_2 PF01469 600 - 633 2e-08 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 861, 1583
AccII CGCG 1 cut(s) 320
AciI CCGC 7 cut(s) 318, 320, 363, 843, 915, 1094, 1321
AclWI GGATC 2 cut(s) 1124, 1499
AcoI YGGCCR 1 cut(s) 1300
AcuI CTGAAG 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 639
AfaI GTAC 7 cut(s) 210, 263, 506, 535, 901, 919, 1388
AfiI CCNNNNNNNGG 2 cut(s) 562, 843
AgsI TTSAA 5 cut(s) 191, 772, 980, 1091, 1198
AjnI CCWGG 2 cut(s) 1228, 1315
AluBI AGCT 6 cut(s) 89, 372, 620, 775, 1355, 1523
AluI AGCT 6 cut(s) 89, 372, 620, 775, 1355, 1523
Alw26I GTCTC 3 cut(s) 189, 194, 964
AlwI GGATC 2 cut(s) 1124, 1499
AlwNI CAGNNNCTG 1 cut(s) 92
AoxI GGCC 2 cut(s) 1165, 1300
ApeKI GCWGC 6 cut(s) 338, 617, 1689, 1719, 1794, 1824
ArsI GACNNNNNNTTYG 2 cut(s) 1406, 1438
AspLEI GCGC 4 cut(s) 617, 781, 1799, 1829
AspS9I GGNCC 3 cut(s) 238, 1062, 1166
AsuC2I CCSGG 1 cut(s) 1467
AsuHPI GGTGA 7 cut(s) 448, 463, 883, 1102, 1244, 1305, 1561
AvaII GGWCC 2 cut(s) 238, 1062
AxyI CCTNAGG 1 cut(s) 590
BalI TGGCCA 1 cut(s) 1302
BbsI GAAGAC 1 cut(s) 1576
BbvI GCAGC 6 cut(s) 325, 629, 1701, 1731, 1806, 1836
BccI CCATC 4 cut(s) 71, 146, 1063, 1109
BceAI ACGGC 2 cut(s) 305, 626
BciT130I CCWGG 2 cut(s) 1230, 1317
BciVI GTATCC 1 cut(s) 1426
BclI TGATCA 2 cut(s) 54, 162
BcnI CCSGG 1 cut(s) 1467
BcoDI GTCTC 3 cut(s) 189, 194, 964
BfaI CTAG 2 cut(s) 429, 1290
BfmI CTRYAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 782
BfuI GTATCC 1 cut(s) 1426
BisI GCNGC 8 cut(s) 321, 339, 618, 1322, 1690, 1720, 1795, 1825
BlsI GCNGC 8 cut(s) 322, 340, 619, 1323, 1691, 1721, 1796, 1826
Bme1390I CCNGG 3 cut(s) 1230, 1317, 1467
Bme18I GGWCC 2 cut(s) 238, 1062
BmgT120I GGNCC 3 cut(s) 238, 1062, 1166
BmiI GGNNCC 2 cut(s) 240, 324
BmrFI CCNGG 3 cut(s) 1230, 1317, 1467
BmrI ACTGGG 2 cut(s) 276, 307
BmsI GCATC 3 cut(s) 482, 837, 940
BmuI ACTGGG 2 cut(s) 276, 307
BpiI GAAGAC 1 cut(s) 1576
Bpu10I CCTNAGC 3 cut(s) 216, 1428, 1519
BpuMI CCSGG 1 cut(s) 1467
BsaAI YACGTR 1 cut(s) 1276
BsaHI GRCGYC 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 189
BsaJI CCNNGG 4 cut(s) 318, 963, 1228, 1316
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 562, 843
Bse1I ACTGG 7 cut(s) 216, 271, 302, 1181, 1303, 1303, 1900
Bse21I CCTNAGG 1 cut(s) 590
BseBI CCWGG 2 cut(s) 1230, 1317
BseDI CCNNGG 4 cut(s) 318, 963, 1228, 1316
BseGI GGATG 5 cut(s) 497, 852, 930, 973, 1015
BseLI CCNNNNNNNGG 2 cut(s) 562, 843
BseMII CTCAG 1 cut(s) 604
BseNI ACTGG 7 cut(s) 216, 271, 302, 1181, 1303, 1303, 1900
BseRI GAGGAG 1 cut(s) 917
BseXI GCAGC 6 cut(s) 325, 629, 1701, 1731, 1806, 1836
BsgI GTGCAG 2 cut(s) 723, 1402
Bsh1236I CGCG 1 cut(s) 320
BshFI GGCC 2 cut(s) 1167, 1302
BsiSI CCGG 1 cut(s) 1467
BslFI GGGAC 2 cut(s) 1211, 1449
BslI CCNNNNNNNGG 2 cut(s) 562, 843
BsmAI GTCTC 3 cut(s) 189, 194, 964
BsmFI GGGAC 2 cut(s) 1211, 1449
BsnI GGCC 2 cut(s) 1167, 1302
Bso31I GGTCTC 1 cut(s) 189
Bsp1407I TGTACA 2 cut(s) 504, 899
Bsp19I CCATGG 1 cut(s) 963
BspACI CCGC 7 cut(s) 318, 320, 363, 843, 915, 1094, 1321
BspANI GGCC 2 cut(s) 1167, 1302
BspCNI CTCAG 1 cut(s) 603
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 2 cut(s) 240, 324
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 2 cut(s) 1124, 1499
BspTNI GGTCTC 1 cut(s) 189
BsrGI TGTACA 2 cut(s) 504, 899
BsrI ACTGG 7 cut(s) 216, 271, 302, 1181, 1303, 1303, 1900
BssECI CCNNGG 4 cut(s) 318, 963, 1228, 1316
BssNAI GTATAC 2 cut(s) 862, 1584
BssNI GRCGYC 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 963
Bst1107I GTATAC 2 cut(s) 862, 1584
Bst2UI CCWGG 2 cut(s) 1230, 1317
Bst4CI ACNGT 7 cut(s) 533, 1142, 1380, 1391, 1559, 1601, 1616
Bst6I CTCTTC 1 cut(s) 192
BstACI GRCGYC 1 cut(s) 639
BstAUI TGTACA 2 cut(s) 504, 899
BstBAI YACGTR 1 cut(s) 1276
BstC8I GCNNGC 1 cut(s) 777
BstDEI CTNAG 6 cut(s) 216, 590, 611, 621, 1428, 1519
BstDSI CCRYGG 2 cut(s) 318, 963
BstENI CCTNNNNNAGG 1 cut(s) 560
BstF5I GGATG 5 cut(s) 497, 852, 930, 973, 1015
BstFNI CGCG 1 cut(s) 320
BstH2I RGCGCY 1 cut(s) 782
BstHHI GCGC 4 cut(s) 617, 781, 1799, 1829
BstMAI GTCTC 3 cut(s) 189, 194, 964
BstMWI GCNNNNNNNGC 4 cut(s) 369, 1001, 1091, 1409
BstNI CCWGG 2 cut(s) 1230, 1317
BstNSI RCATGY 2 cut(s) 957, 1054
BstSCI CCNGG 3 cut(s) 1228, 1315, 1465
BstSFI CTRYAG 1 cut(s) 93
BstSNI TACGTA 1 cut(s) 1276
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 6 cut(s) 325, 629, 1701, 1731, 1806, 1836
BstV2I GAAGAC 1 cut(s) 1576
BstX2I RGATCY 1 cut(s) 1491
BstYI RGATCY 1 cut(s) 1491
BstZ17I GTATAC 2 cut(s) 862, 1584
Bsu36I CCTNAGG 1 cut(s) 590
BsuI GTATCC 1 cut(s) 1426
BsuRI GGCC 2 cut(s) 1167, 1302
BtgI CCRYGG 2 cut(s) 318, 963
BtsCI GGATG 5 cut(s) 497, 852, 930, 973, 1015
BtsI GCAGTG 9 cut(s) 999, 1636, 1651, 1681, 1711, 1741, 1771, 1861, 1891
Cac8I GCNNGC 1 cut(s) 777
CaiI CAGNNNCTG 1 cut(s) 92
CfoI GCGC 4 cut(s) 617, 781, 1799, 1829
Cfr13I GGNCC 3 cut(s) 238, 1062, 1166
Cfr42I CCGCGG 1 cut(s) 321
CseI GACGC 1 cut(s) 647
Csp6I GTAC 7 cut(s) 209, 262, 505, 534, 900, 918, 1387
CviQI GTAC 7 cut(s) 209, 262, 505, 534, 900, 918, 1387
DdeI CTNAG 6 cut(s) 216, 590, 611, 621, 1428, 1519
DraI TTTAAA 1 cut(s) 1620
EaeI YGGCCR 1 cut(s) 1300
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Eco105I TACGTA 1 cut(s) 1276
Eco130I CCWWGG 1 cut(s) 963
Eco31I GGTCTC 1 cut(s) 189
Eco47I GGWCC 2 cut(s) 238, 1062
Eco57I CTGAAG 1 cut(s) 288
Eco81I CCTNAGG 1 cut(s) 590
EcoNI CCTNNNNNAGG 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 238
EcoRII CCWGG 2 cut(s) 1228, 1315
EcoT14I CCWWGG 1 cut(s) 963
EcoT22I ATGCAT 1 cut(s) 955
ErhI CCWWGG 1 cut(s) 963
FaqI GGGAC 2 cut(s) 1211, 1449
FauI CCCGC 2 cut(s) 356, 836
FbaI TGATCA 2 cut(s) 54, 162
FblI GTMKAC 2 cut(s) 861, 1583
Fnu4HI GCNGC 8 cut(s) 321, 339, 618, 1322, 1690, 1720, 1795, 1825
FokI GGATG 5 cut(s) 504, 859, 917, 980, 1022
Fsp4HI GCNGC 8 cut(s) 321, 339, 618, 1322, 1690, 1720, 1795, 1825
FspBI CTAG 2 cut(s) 429, 1290
GlaI GCGC 4 cut(s) 616, 780, 1798, 1828
GluI GCNGC 8 cut(s) 321, 339, 618, 1322, 1690, 1720, 1795, 1825
HaeII RGCGCY 1 cut(s) 782
HaeIII GGCC 2 cut(s) 1167, 1302
HapII CCGG 1 cut(s) 1467
HgaI GACGC 1 cut(s) 647
HhaI GCGC 4 cut(s) 617, 781, 1799, 1829
Hin1I GRCGYC 1 cut(s) 639
Hin6I GCGC 4 cut(s) 615, 779, 1797, 1827
HinP1I GCGC 4 cut(s) 615, 779, 1797, 1827
HindIII AAGCTT 1 cut(s) 370
HinfI GANTC 5 cut(s) 605, 667, 886, 976, 1327
HpaII CCGG 1 cut(s) 1467
HphI GGTGA 7 cut(s) 448, 463, 883, 1102, 1244, 1305, 1561
Hpy166II GTNNAC 7 cut(s) 264, 862, 1030, 1363, 1454, 1584, 1604
Hpy188I TCNGA 9 cut(s) 125, 604, 632, 654, 666, 721, 1138, 1218, 1444
Hpy188III TCNNGA 6 cut(s) 191, 355, 429, 592, 1127, 1562
Hpy8I GTNNAC 7 cut(s) 264, 862, 1030, 1363, 1454, 1584, 1604
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 7 cut(s) 79, 171, 229, 556, 818, 1143, 1204
HpyCH4III ACNGT 7 cut(s) 533, 1142, 1380, 1391, 1559, 1601, 1616
HpyCH4IV ACGT 2 cut(s) 1275, 1365
HpyF10VI GCNNNNNNNGC 4 cut(s) 369, 1001, 1091, 1409
HpyF3I CTNAG 6 cut(s) 216, 590, 611, 621, 1428, 1519
HpySE526I ACGT 2 cut(s) 1275, 1365
Hsp92I GRCGYC 1 cut(s) 639
HspAI GCGC 4 cut(s) 615, 779, 1797, 1827
Ksp22I TGATCA 2 cut(s) 54, 162
KspI CCGCGG 1 cut(s) 321
LmnI GCTCC 2 cut(s) 328, 810
Lsp1109I GCAGC 6 cut(s) 325, 629, 1701, 1731, 1806, 1836
LweI GCATC 3 cut(s) 482, 837, 940
MaeI CTAG 2 cut(s) 429, 1290
MaeII ACGT 2 cut(s) 1275, 1365
MaeIII GTNAC 3 cut(s) 454, 892, 1595
MboII GAAGA 6 cut(s) 179, 186, 460, 637, 1534, 1581
MfeI CAATTG 1 cut(s) 483
MflI RGATCY 1 cut(s) 1491
MlsI TGGCCA 1 cut(s) 1302
MluCI AATT 6 cut(s) 342, 483, 672, 709, 1150, 1241
MluNI TGGCCA 1 cut(s) 1302
MlyI GAGTC 1 cut(s) 1321
MmeI TCCRAC 3 cut(s) 677, 1035, 1319
Mox20I TGGCCA 1 cut(s) 1302
Mph1103I ATGCAT 1 cut(s) 955
MscI TGGCCA 1 cut(s) 1302
MseI TTAA 7 cut(s) 255, 368, 761, 1098, 1148, 1508, 1619
MslI CAYNNNNRTG 1 cut(s) 476
Msp20I TGGCCA 1 cut(s) 1302
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 1 cut(s) 1467
MspR9I CCNGG 3 cut(s) 1230, 1317, 1467
MssI GTTTAAAC 1 cut(s) 1620
MunI CAATTG 1 cut(s) 483
MvaI CCWGG 2 cut(s) 1230, 1317
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 4 cut(s) 369, 1001, 1091, 1409
NciI CCSGG 1 cut(s) 1467
NcoI CCATGG 1 cut(s) 963
NlaIV GGNNCC 2 cut(s) 240, 324
NmuCI GTSAC 2 cut(s) 454, 1595
NsiI ATGCAT 1 cut(s) 955
NspI RCATGY 2 cut(s) 957, 1054
PcsI WCGNNNNNNNCGW 2 cut(s) 26, 1573
PfeI GAWTC 4 cut(s) 605, 667, 886, 976
PkrI GCNGC 8 cut(s) 322, 340, 619, 1323, 1691, 1721, 1796, 1826
PleI GAGTC 1 cut(s) 1321
PmeI GTTTAAAC 1 cut(s) 1620
PpsI GAGTC 1 cut(s) 1321
Ppu21I YACGTR 1 cut(s) 1276
PpuMI RGGWCCY 1 cut(s) 238
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 2 cut(s) 1228, 1315
PspGI CCWGG 2 cut(s) 1228, 1315
PspN4I GGNNCC 2 cut(s) 240, 324
PspPI GGNCC 3 cut(s) 238, 1062, 1166
PspPPI RGGWCCY 1 cut(s) 238
PstI CTGCAG 1 cut(s) 97
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 1 cut(s) 1491
RsaI GTAC 7 cut(s) 210, 263, 506, 535, 901, 919, 1388
RsaNI GTAC 7 cut(s) 209, 262, 505, 534, 900, 918, 1387
RseI CAYNNNNRTG 1 cut(s) 476
SacII CCGCGG 1 cut(s) 321
SaqAI TTAA 7 cut(s) 255, 368, 761, 1098, 1148, 1508, 1619
SatI GCNGC 8 cut(s) 321, 339, 618, 1322, 1690, 1720, 1795, 1825
Sau96I GGNCC 3 cut(s) 238, 1062, 1166
SchI GAGTC 1 cut(s) 1321
ScrFI CCNGG 3 cut(s) 1230, 1317, 1467
SfaNI GCATC 3 cut(s) 482, 837, 940
SfcI CTRYAG 1 cut(s) 93
Sfr303I CCGCGG 1 cut(s) 321
SgrBI CCGCGG 1 cut(s) 321
SinI GGWCC 2 cut(s) 238, 1062
SmiMI CAYNNNNRTG 1 cut(s) 476
SnaBI TACGTA 1 cut(s) 1276
Sse9I AATT 6 cut(s) 342, 483, 672, 709, 1150, 1241
SsiI CCGC 7 cut(s) 318, 320, 363, 843, 915, 1094, 1321
SspMI CTAG 2 cut(s) 429, 1290
StyD4I CCNGG 3 cut(s) 1228, 1315, 1465
StyI CCWWGG 1 cut(s) 963
TaaI ACNGT 7 cut(s) 533, 1142, 1380, 1391, 1559, 1601, 1616
TaiI ACGT 2 cut(s) 1278, 1368
TaqI TCGA 3 cut(s) 32, 1341, 1663
TasI AATT 6 cut(s) 342, 483, 672, 709, 1150, 1241
TatI WGTACW 4 cut(s) 261, 504, 533, 899
TauI GCSGC 2 cut(s) 323, 1324
TfiI GAWTC 4 cut(s) 605, 667, 886, 976
Tru1I TTAA 7 cut(s) 255, 368, 761, 1098, 1148, 1508, 1619
Tru9I TTAA 7 cut(s) 255, 368, 761, 1098, 1148, 1508, 1619
TseFI GTSAC 2 cut(s) 454, 1595
TseI GCWGC 6 cut(s) 338, 617, 1689, 1719, 1794, 1824
Tsp45I GTSAC 2 cut(s) 454, 1595
TspDTI ATGAA 2 cut(s) 588, 895
TspGWI ACGGA 3 cut(s) 244, 757, 1075
VpaK11BI GGWCC 2 cut(s) 238, 1062
XagI CCTNNNNNAGG 1 cut(s) 560
XbaI TCTAGA 1 cut(s) 428
XceI RCATGY 2 cut(s) 957, 1054
XcmI CCANNNNNNNNNTGG 1 cut(s) 941
XmiI GTMKAC 2 cut(s) 861, 1583
XspI CTAG 2 cut(s) 429, 1290
Zsp2I ATGCAT 1 cut(s) 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.