Rw2G001860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012554
Physical Location & Seq
Forward (+)
1530812 .. 1534341
3530 bp
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UTR
Exon/CDS
Intron
Rw2G001860.1

Sequence Viewer

Length: 1428 bp
ATGTTGCTGTTGGCTCCCTTCAATGTTCGCGTGGTCTGCTGTAGTGCTGGATGCGTTGTGCTGTACATGGGTCAGGGATTGTTCTACGACAGTACAAGAAAGACATTGGATTACCTTGTGAAGGAGGCAGATGAAACTGCTCAAAACCTCAGGAATGTGTCAGATTCTCTTAGCGCAGCTAAGAAGATCGGACTTGACGCCATAGTTCCTCCGACAGATATTCAGAATCAAATTGACGAGGGTATGGGAACAATACAGTATGTTTCCAATTTTCTTTCTGAAACAACTGATAAGACTGCACGGAAGTCAAATCAAGTCTTAACAGACTTGAAGCTGGCGCTCATGGTTTTTTCTGCTGTTATGCTCCTTCTGGTCTTTATTGGATTTGCACTTTCCATGTGCGGGATGCGATTTCTTGTATACTGCTTGGTGATTGTTGGGTGGATTCTTGTTACCTGTGCATTCATCTTGTGCGGTACATTTCTCCTCATCCATAATGCGTTTGGAGATGCATGTGTCTCCATGGATGAGTGGATTCAAAAGCACCCCACAGCCCACACTGCTTTGGATGATCTACTTCCATGCGTGGACAAAAATGTAAGCCAGAGCATGTTGGAACGGACCAAGATGGCAACCTACTTTGCTGTTGAAGCGGTTAATCGTGTCATCACCGATGGTGCAAATCTTGATCCTTCTGACAGTCCATTAAATTACAATCAGTCTGGCCCTTTATTACCACCAGTCTGCAACCCCTTTAATGAGGATAAAACTGATCGGAAATGTGTTCCTGGTGAAGTGGAATTGCAACAAGCCCAAGAGGTATGGCAGAAATACGTATGTAAGGTTTCTAGTCCAACCGGCCAGTGTGTATCACCAGGGCGGCTGACTCCTACCTACTTCGATCAAATGTCAGCTTTTGTAAATGTGAGCAACGCACTGTATCGGTACAGTCCATTCTTTGCTGATCTTGCAGACTGCACATTTGCTAAGGATACATTCTCGGACATAAGTAAACAGCATTGTCCCGGTCTGCGACTACATAGTAAATGGATCTACATTGGCTTGTTAATGGTAGCCTTAGCTGTAATGTTGTCTATGATCTTCTGGGTGATTTACTGCCGGGAACGAAGACATCGCGTGTATACTAAAAAATGTGACAGTTTACACTATGACAGTTTAAACTATGGCAGTGCAAATCATGGCAGTAGAAACTTTGGCAGTGCAAATCATGGCAGCAGAAACTTTGGCAGTGGAAATCATGGCAGCAGAAACTTTGGCAGCCCAAATCATGGCAGTAGAAACTTTGGCAGCCCAAATCATGGCAGTAGAAACTTTGGCAGTGGAAATCATGGCAGTGGAAACTTTGGCCCAAATCATGGCAGTAGAAACTTTGGCAGTGGAAACTTTGGCAGTGCAAATACTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

475

Amino Acids

52.24

Weight (kDa)

7.84

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pentapeptide_2 PF01469 393 - 427 1.7e-08 Pentapeptide repeats (8 copies)
Pentapeptide_2 PF01469 418 - 456 3e-08 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 1289, 1319, 1376
AccI GTMKAC 2 cut(s) 420, 1142
AccII CGCG 2 cut(s) 30, 1137
AciI CCGC 4 cut(s) 402, 474, 653, 880
AclWI GGATC 2 cut(s) 683, 1058
AcoI YGGCCR 1 cut(s) 859
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 4 cut(s) 65, 94, 478, 947
AfiI CCNNNNNNNGG 5 cut(s) 121, 402, 1289, 1319, 1376
AgsI TTSAA 4 cut(s) 22, 331, 539, 650
AjnI CCWGG 2 cut(s) 787, 874
AluBI AGCT 4 cut(s) 179, 334, 914, 1082
AluI AGCT 4 cut(s) 179, 334, 914, 1082
Alw26I GTCTC 1 cut(s) 523
AlwI GGATC 2 cut(s) 683, 1058
AoxI GGCC 3 cut(s) 724, 859, 1366
ApeKI GCWGC 5 cut(s) 176, 1233, 1263, 1278, 1308
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
AspLEI GCGC 2 cut(s) 176, 340
AspS9I GGNCC 3 cut(s) 621, 725, 1367
AsuC2I CCSGG 2 cut(s) 1026, 1121
AsuHPI GGTGA 5 cut(s) 442, 661, 803, 864, 1120
AvaII GGWCC 1 cut(s) 621
AxyI CCTNAGG 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 1135
BbvI GCAGC 5 cut(s) 188, 1245, 1275, 1290, 1320
BccI CCATC 2 cut(s) 622, 668
BciT130I CCWGG 2 cut(s) 789, 876
BciVI GTATCC 1 cut(s) 985
BcnI CCSGG 2 cut(s) 1026, 1121
BcoDI GTCTC 1 cut(s) 523
BfaI CTAG 1 cut(s) 849
BfmI CTRYAG 1 cut(s) 40
BfoI RGCGCY 1 cut(s) 341
BfuI GTATCC 1 cut(s) 985
BisI GCNGC 6 cut(s) 177, 881, 1234, 1264, 1279, 1309
BlsI GCNGC 6 cut(s) 178, 882, 1235, 1265, 1280, 1310
Bme1390I CCNGG 4 cut(s) 789, 876, 1026, 1121
Bme18I GGWCC 1 cut(s) 621
BmgT120I GGNCC 3 cut(s) 621, 725, 1367
BmiI GGNNCC 1 cut(s) 15
BmrFI CCNGG 4 cut(s) 789, 876, 1026, 1121
BmsI GCATC 3 cut(s) 41, 396, 499
BpiI GAAGAC 1 cut(s) 1135
Bpu10I CCTNAGC 2 cut(s) 987, 1078
BpuMI CCSGG 2 cut(s) 1026, 1121
BsaAI YACGTR 1 cut(s) 835
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 522, 875
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 402, 1289, 1319, 1376
Bse118I RCCGGY 1 cut(s) 857
Bse1I ACTGG 3 cut(s) 740, 862, 1426
Bse21I CCTNAGG 1 cut(s) 149
BseBI CCWGG 2 cut(s) 789, 876
BseDI CCNNGG 2 cut(s) 522, 875
BseGI GGATG 5 cut(s) 56, 411, 489, 532, 574
BseLI CCNNNNNNNGG 5 cut(s) 121, 402, 1289, 1319, 1376
BseMII CTCAG 1 cut(s) 163
BseNI ACTGG 3 cut(s) 740, 862, 1426
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 5 cut(s) 188, 1245, 1275, 1290, 1320
BsgI GTGCAG 2 cut(s) 282, 961
Bsh1236I CGCG 2 cut(s) 30, 1137
BshFI GGCC 3 cut(s) 726, 861, 1368
BsiSI CCGG 3 cut(s) 858, 1026, 1120
BslFI GGGAC 1 cut(s) 1008
BslI CCNNNNNNNGG 5 cut(s) 121, 402, 1289, 1319, 1376
BsmAI GTCTC 1 cut(s) 523
BsmFI GGGAC 1 cut(s) 1008
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 3 cut(s) 726, 861, 1368
Bsp1407I TGTACA 1 cut(s) 63
Bsp143I GATC 8 cut(s) 186, 571, 688, 772, 901, 964, 1050, 1098
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 4 cut(s) 402, 474, 653, 880
BspANI GGCC 3 cut(s) 726, 861, 1368
BspCNI CTCAG 1 cut(s) 162
BspFNI CGCG 2 cut(s) 30, 1137
BspLI GGNNCC 1 cut(s) 15
BspPI GGATC 2 cut(s) 683, 1058
BsrFI RCCGGY 1 cut(s) 857
BsrGI TGTACA 1 cut(s) 63
BsrI ACTGG 3 cut(s) 740, 862, 1426
BssAI RCCGGY 1 cut(s) 857
BssECI CCNNGG 2 cut(s) 522, 875
BssMI GATC 8 cut(s) 186, 571, 688, 772, 901, 964, 1050, 1098
BssNAI GTATAC 2 cut(s) 421, 1143
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 522
Bst1107I GTATAC 2 cut(s) 421, 1143
Bst2UI CCWGG 2 cut(s) 789, 876
Bst4CI ACNGT 7 cut(s) 92, 258, 701, 939, 950, 1160, 1175
BstACI GRCGYC 1 cut(s) 198
BstAUI TGTACA 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 835
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 5 cut(s) 149, 170, 180, 987, 1078
BstDSI CCRYGG 1 cut(s) 522
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 5 cut(s) 56, 411, 489, 532, 574
BstFNI CGCG 2 cut(s) 30, 1137
BstH2I RGCGCY 1 cut(s) 341
BstHHI GCGC 2 cut(s) 176, 340
BstKTI GATC 8 cut(s) 189, 574, 691, 775, 904, 967, 1053, 1101
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 8 cut(s) 186, 571, 688, 772, 901, 964, 1050, 1098
BstMWI GCNNNNNNNGC 4 cut(s) 36, 560, 650, 968
BstNI CCWGG 2 cut(s) 789, 876
BstNSI RCATGY 2 cut(s) 516, 613
BstSCI CCNGG 4 cut(s) 787, 874, 1024, 1119
BstSFI CTRYAG 1 cut(s) 40
BstSNI TACGTA 1 cut(s) 835
BstUI CGCG 2 cut(s) 30, 1137
BstV1I GCAGC 5 cut(s) 188, 1245, 1275, 1290, 1320
BstV2I GAAGAC 1 cut(s) 1135
BstX2I RGATCY 1 cut(s) 1050
BstYI RGATCY 1 cut(s) 1050
BstZ17I GTATAC 2 cut(s) 421, 1143
Bsu36I CCTNAGG 1 cut(s) 149
BsuI GTATCC 1 cut(s) 985
BsuRI GGCC 3 cut(s) 726, 861, 1368
BtgI CCRYGG 1 cut(s) 522
BtgZI GCGATG 1 cut(s) 1118
BtsCI GGATG 5 cut(s) 56, 411, 489, 532, 574
BtsI GCAGTG 8 cut(s) 558, 1195, 1225, 1255, 1345, 1360, 1402, 1417
Cac8I GCNNGC 1 cut(s) 336
CfoI GCGC 2 cut(s) 176, 340
Cfr10I RCCGGY 1 cut(s) 857
Cfr13I GGNCC 3 cut(s) 621, 725, 1367
CseI GACGC 1 cut(s) 206
Csp6I GTAC 4 cut(s) 64, 93, 477, 946
CviQI GTAC 4 cut(s) 64, 93, 477, 946
DdeI CTNAG 5 cut(s) 149, 170, 180, 987, 1078
DpnI GATC 8 cut(s) 188, 573, 690, 774, 903, 966, 1052, 1100
DpnII GATC 8 cut(s) 186, 571, 688, 772, 901, 964, 1050, 1098
DraI TTTAAA 1 cut(s) 1179
EaeI YGGCCR 1 cut(s) 859
Eco105I TACGTA 1 cut(s) 835
Eco130I CCWWGG 1 cut(s) 522
Eco47I GGWCC 1 cut(s) 621
Eco81I CCTNAGG 1 cut(s) 149
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoRII CCWGG 2 cut(s) 787, 874
EcoT14I CCWWGG 1 cut(s) 522
EcoT22I ATGCAT 1 cut(s) 514
ErhI CCWWGG 1 cut(s) 522
FaqI GGGAC 1 cut(s) 1008
FauI CCCGC 1 cut(s) 395
FblI GTMKAC 2 cut(s) 420, 1142
Fnu4HI GCNGC 6 cut(s) 177, 881, 1234, 1264, 1279, 1309
FokI GGATG 5 cut(s) 63, 418, 476, 539, 581
Fsp4HI GCNGC 6 cut(s) 177, 881, 1234, 1264, 1279, 1309
FspBI CTAG 1 cut(s) 849
GlaI GCGC 2 cut(s) 175, 339
GluI GCNGC 6 cut(s) 177, 881, 1234, 1264, 1279, 1309
HaeII RGCGCY 1 cut(s) 341
HaeIII GGCC 3 cut(s) 726, 861, 1368
HapII CCGG 3 cut(s) 858, 1026, 1120
HgaI GACGC 1 cut(s) 206
HhaI GCGC 2 cut(s) 176, 340
Hin1I GRCGYC 1 cut(s) 198
Hin6I GCGC 2 cut(s) 174, 338
HinP1I GCGC 2 cut(s) 174, 338
HinfI GANTC 5 cut(s) 164, 226, 445, 535, 886
HpaII CCGG 3 cut(s) 858, 1026, 1120
HphI GGTGA 5 cut(s) 442, 661, 803, 864, 1120
Hpy166II GTNNAC 5 cut(s) 421, 589, 1013, 1143, 1163
Hpy188I TCNGA 8 cut(s) 163, 191, 213, 225, 280, 697, 777, 1003
Hpy188III TCNNGA 2 cut(s) 151, 686
Hpy8I GTNNAC 5 cut(s) 421, 589, 1013, 1143, 1163
HpyAV CCTTC 4 cut(s) 28, 115, 377, 702
HpyCH4III ACNGT 7 cut(s) 92, 258, 701, 939, 950, 1160, 1175
HpyCH4IV ACGT 1 cut(s) 834
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 560, 650, 968
HpyF3I CTNAG 5 cut(s) 149, 170, 180, 987, 1078
HpySE526I ACGT 1 cut(s) 834
Hsp92I GRCGYC 1 cut(s) 198
HspAI GCGC 2 cut(s) 174, 338
Kzo9I GATC 8 cut(s) 186, 571, 688, 772, 901, 964, 1050, 1098
LmnI GCTCC 2 cut(s) 19, 369
Lsp1109I GCAGC 5 cut(s) 188, 1245, 1275, 1290, 1320
LweI GCATC 3 cut(s) 41, 396, 499
MaeI CTAG 1 cut(s) 849
MaeII ACGT 1 cut(s) 834
MaeIII GTNAC 2 cut(s) 451, 1154
MalI GATC 8 cut(s) 188, 573, 690, 774, 903, 966, 1052, 1100
MboI GATC 8 cut(s) 186, 571, 688, 772, 901, 964, 1050, 1098
MboII GAAGA 3 cut(s) 196, 1093, 1140
MflI RGATCY 1 cut(s) 1050
MluCI AATT 4 cut(s) 231, 268, 709, 800
MlyI GAGTC 1 cut(s) 880
MmeI TCCRAC 3 cut(s) 236, 594, 878
MnlI CCTC 7 cut(s) 118, 158, 219, 232, 497, 754, 811
Mph1103I ATGCAT 1 cut(s) 514
MseI TTAA 6 cut(s) 320, 657, 707, 756, 1067, 1178
MslI CAYNNNNRTG 1 cut(s) 1353
MspI CCGG 3 cut(s) 858, 1026, 1120
MspR9I CCNGG 4 cut(s) 789, 876, 1026, 1121
MssI GTTTAAAC 1 cut(s) 1179
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 2 cut(s) 789, 876
MvnI CGCG 2 cut(s) 30, 1137
MwoI GCNNNNNNNGC 4 cut(s) 36, 560, 650, 968
NciI CCSGG 2 cut(s) 1026, 1121
NcoI CCATGG 1 cut(s) 522
NdeII GATC 8 cut(s) 186, 571, 688, 772, 901, 964, 1050, 1098
NlaIV GGNNCC 1 cut(s) 15
NmuCI GTSAC 1 cut(s) 1154
NsiI ATGCAT 1 cut(s) 514
NspI RCATGY 2 cut(s) 516, 613
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 4 cut(s) 164, 226, 445, 535
PflMI CCANNNNNTGG 3 cut(s) 1289, 1319, 1376
PkrI GCNGC 6 cut(s) 178, 882, 1235, 1265, 1280, 1310
PleI GAGTC 1 cut(s) 880
PmeI GTTTAAAC 1 cut(s) 1179
PpsI GAGTC 1 cut(s) 880
Ppu21I YACGTR 1 cut(s) 835
Psp6I CCWGG 2 cut(s) 787, 874
PspGI CCWGG 2 cut(s) 787, 874
PspN4I GGNNCC 1 cut(s) 15
PspPI GGNCC 3 cut(s) 621, 725, 1367
PsuI RGATCY 1 cut(s) 1050
RsaI GTAC 4 cut(s) 65, 94, 478, 947
RsaNI GTAC 4 cut(s) 64, 93, 477, 946
RseI CAYNNNNRTG 1 cut(s) 1353
SaqAI TTAA 6 cut(s) 320, 657, 707, 756, 1067, 1178
SatI GCNGC 6 cut(s) 177, 881, 1234, 1264, 1279, 1309
Sau3AI GATC 8 cut(s) 186, 571, 688, 772, 901, 964, 1050, 1098
Sau96I GGNCC 3 cut(s) 621, 725, 1367
SchI GAGTC 1 cut(s) 880
ScrFI CCNGG 4 cut(s) 789, 876, 1026, 1121
SfaNI GCATC 3 cut(s) 41, 396, 499
SfcI CTRYAG 1 cut(s) 40
SinI GGWCC 1 cut(s) 621
SmiMI CAYNNNNRTG 1 cut(s) 1353
SnaBI TACGTA 1 cut(s) 835
Sse9I AATT 4 cut(s) 231, 268, 709, 800
SsiI CCGC 4 cut(s) 402, 474, 653, 880
SspMI CTAG 1 cut(s) 849
StyD4I CCNGG 4 cut(s) 787, 874, 1024, 1119
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 7 cut(s) 92, 258, 701, 939, 950, 1160, 1175
TaiI ACGT 1 cut(s) 837
TaqI TCGA 1 cut(s) 900
TasI AATT 4 cut(s) 231, 268, 709, 800
TatI WGTACW 2 cut(s) 63, 92
TauI GCSGC 1 cut(s) 883
TfiI GAWTC 4 cut(s) 164, 226, 445, 535
Tru1I TTAA 6 cut(s) 320, 657, 707, 756, 1067, 1178
Tru9I TTAA 6 cut(s) 320, 657, 707, 756, 1067, 1178
TseFI GTSAC 1 cut(s) 1154
TseI GCWGC 5 cut(s) 176, 1233, 1263, 1278, 1308
Tsp45I GTSAC 1 cut(s) 1154
TspDTI ATGAA 2 cut(s) 147, 454
TspGWI ACGGA 2 cut(s) 316, 634
Van91I CCANNNNNTGG 3 cut(s) 1289, 1319, 1376
VpaK11BI GGWCC 1 cut(s) 621
XagI CCTNNNNNAGG 1 cut(s) 119
XceI RCATGY 2 cut(s) 516, 613
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmiI GTMKAC 2 cut(s) 420, 1142
XspI CTAG 1 cut(s) 849
Zsp2I ATGCAT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.