RLG00000016091

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006901
Physical Location & Seq
Reverse (-)
3807834 .. 3809702
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016091

Sequence Viewer

Length: 1752 bp
ATGAAATCAAGCTCAACTCCGGTATCTCGCTGCCTTGTTTGTTTGGACAAGAACAGTTGTCGAGTAGTTCGTTCGAGAACAAAGCTGATGAATAATTTAATTGCAAGAGGGAAGCCTCATGAAGCTCATTCCATCTTCAATAATTTAGTAGAAGAAGGACATAGGCCAACTCTTATAACATACACAACTCTAGTAGCTGCCTTGACCCGCCAGAGGCGTTTCAAGTCAATTCCCTTGCTCCTCTCTGAAGTGGAGGAGAATGGTATGAAGCCTGATTCAATACTTTTCAATGCCATGATCAATGCTTTCTCCGAATCTGGAAATGTAAATGATGCCATGAAAATCTTTCGCAAAATGGAGGAGAGTGGATGTAAGCCCACAACCAGCACTTTTAACACCCTTATCAAAGGATATGGAATTGCTGGTAAACCTGAAGAATCTTTAAAATTGCTAGAGCTTATGTTGCAGGATGGGAATGCGAAACCGAATGACAGGACATATAATATTCTAGTTAGAGCCTGGTGTAACAAGAAGAGATTAGATCAAGCCTGGAATGTGGTGTATAAGATGGTTGCTTCTGGGATACAACCTGATGCAGTCACTTTCAACACATTGGCAAGGGCTTATGCTGAGAATAGAGAGACCAGTAAAGCTGAAAGGTTGATGTTGGAGATGCAGCACAACAAAGTTAACCCCAATGAGCGTACGTGTGGCATTGTTGTAAATGGATATTGTAAAGAAGGTAACATGACAGATGCTCTGAGGTTTGTATACAGAATGAAGGATCTTGGGGTGAATCCAAATCTTGTCATTTTCAACTCTCTCATCAAGGGGTTTCTGAACATTACAGACACTGATGGAGTTGATGAGGTACTGACATTGATGGAAGAATTTGGGATAAAACCAGATGTGATAACTTTTAGCACCATAATGAATGCATGGAGCTCAGCAGGGCTAATGGAAAAATGCCAGGAGATCTTTGATGACATGATAAAAGCAGACATAGAACCAGATATCCATTCGTTTGGCATTCTTGCAAAAGGCTATGTGCGTGCCGGTGATCCTGCGAAGGCAGAGTCACTTCTGAGTTTCATGGGCAAATCTGGTGTGCATCCCAATGTTGTAATCTTCACAACCATCATGAGTGGGTGGTGCACAGCAGGAAAAATGGAGCATGCTTGGGTTATCTTTGAGAAAATGTGTGAAATGGGAATCACCCCAAATATGAAAACTTTTGAGACTCTAATATGGGGATATGGGGAAGCTAGGCAGCCATGGAAAGCGGAAGATTTGCTTCATAAAATGGAAGAGAAGGGTATTGTTCCTGAAATGAGAACAATTGAACTTGTTGCTGAGGCTTGGCGTGCTATCGGTTTAATGGATGAAGCGATGAGACTCCTCAATGAGTCAGAAGAGGATTCAGAAGTCATACTAAACAAGGCACATGGACAGAGTTTAGAGACAATTAGTCGGAAACAAAGCTTGAGTATTTCTTATCCTAATGCTTTGCAGATACCTGGAGTAGTTGTTTCTGATAATGGCAGCTCTGCTGCTAACATAAGAGGCCAGATGATTTCAAAACGATTCGAGTTTTCATCTGAAAGTATGCAGAATGTTACTAGAACCATGTGTGTTACTCATACATCTGCACTAAGAGTGCAGCCACTGGTTAAATGCAGGAGGCAGTTTCAAAGTCAAGTTGGGATATGCAGGCAGTTTGAAAATACATGTAGAGTGGTGTATATATGTTAG

Protein Analysis

584

Amino Acids

65.53

Weight (kDa)

8.08

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 27 - 68 2.5e-06 PPR repeat family
PPR_3 PF13812 80 - 139 2.3e-14 Pentatricopeptide repeat domain
PPR_1 PF12854 87 - 120 1.9e-08 PPR repeat
PPR_2 PF13041 91 - 138 5.9e-15 PPR repeat family
PPR PF01535 95 - 124 1.6e-07 PPR repeat
PPR_long PF17177 100 - 198 5.7e-09 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 112 - 219 8e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 123 - 154 3.4e-07 PPR repeat
PPR_2 PF13041 126 - 176 7.4e-11 PPR repeat family
PPR PF01535 129 - 158 8.5e-07 PPR repeat
PPR_1 PF12854 161 - 191 3.6e-06 PPR repeat
PPR_2 PF13041 162 - 210 6.3e-12 PPR repeat family
PPR_long PF17177 186 - 267 1.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 186 - 233 2.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 197 - 246 3.9e-11 PPR repeat family
PPR_2 PF13041 236 - 279 1.3e-07 PPR repeat family
PPR_long PF17177 252 - 357 6.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 260 - 314 6.8e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 267 - 313 3.4e-08 PPR repeat family
PPR_2 PF13041 302 - 350 3.1e-10 PPR repeat family
PPR_3 PF13812 326 - 382 4.1e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 366 - 420 2.9e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 369 - 400 1.2e-08 PPR repeat
PPR_2 PF13041 372 - 419 1.4e-13 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 176
AccI GTMKAC 1 cut(s) 771
AciI CCGC 2 cut(s) 208, 1283
AclWI GGATC 2 cut(s) 792, 1055
AcsI RAATTY 1 cut(s) 890
AcuI CTGAAG 2 cut(s) 267, 453
AfaI GTAC 2 cut(s) 706, 873
AfiI CCNNNNNNNGG 1 cut(s) 213
AflIII ACRYGT 2 cut(s) 707, 1727
AhdI GACNNNNNGTC 1 cut(s) 1467
AjnI CCWGG 4 cut(s) 518, 548, 969, 1516
AloI GAACNNNNNNTCC 2 cut(s) 999, 1031
Alw21I GWGCWC 2 cut(s) 947, 1157
Alw26I GTCTC 4 cut(s) 635, 1232, 1387, 1454
Alw44I GTGCAC 1 cut(s) 1153
AlwI GGATC 2 cut(s) 792, 1055
AlwNI CAGNNNCTG 2 cut(s) 854, 1666
AoxI GGCC 2 cut(s) 164, 1564
ApaLI GTGCAC 1 cut(s) 1153
ApeKI GCWGC 7 cut(s) 30, 197, 676, 1270, 1542, 1550, 1660
ApoI RAATTY 1 cut(s) 890
ArsI GACNNNNNNTTYG 2 cut(s) 1061, 1093
AsuHPI GGTGA 3 cut(s) 805, 1070, 1207
BaeGI GKGCMC 1 cut(s) 1157
BaeI ACNNNNGTAYC 1 cut(s) 39
BanII GRGCYC 1 cut(s) 947
Bbv12I GWGCWC 2 cut(s) 947, 1157
BbvCI CCTCAGC 1 cut(s) 1353
BbvI GCAGC 7 cut(s) 17, 184, 688, 1282, 1537, 1554, 1672
BccI CCATC 6 cut(s) 140, 464, 562, 851, 877, 1145
BciT130I CCWGG 4 cut(s) 520, 550, 971, 1518
BciVI GTATCC 1 cut(s) 576
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 4 cut(s) 635, 1232, 1387, 1454
BfaI CTAG 5 cut(s) 191, 452, 509, 1266, 1620
BfuI GTATCC 1 cut(s) 576
BglII AGATCT 1 cut(s) 975
BisI GCNGC 7 cut(s) 31, 198, 677, 1271, 1543, 1551, 1661
BlpI GCTNAGC 1 cut(s) 946
BlsI GCNGC 7 cut(s) 32, 199, 678, 1272, 1544, 1552, 1662
Bme1390I CCNGG 4 cut(s) 520, 550, 971, 1518
BmeRI GACNNNNNGTC 1 cut(s) 1467
BmrFI CCNGG 4 cut(s) 520, 550, 971, 1518
BmsI GCATC 5 cut(s) 322, 583, 663, 745, 1120
BpmI CTGGAG 1 cut(s) 1539
Bpu10I CCTNAGC 1 cut(s) 1353
Bpu1102I GCTNAGC 1 cut(s) 946
BpuEI CTTGAG 1 cut(s) 1504
BsaAI YACGTR 1 cut(s) 708
BsaI GGTCTC 1 cut(s) 635
BsaJI CCNNGG 1 cut(s) 1274
BsaWI WCCGGW 1 cut(s) 19
BsaXI ACNNNNNCTCC 2 cut(s) 1512, 1542
Bsc4I CCNNNNNNNGG 1 cut(s) 213
Bse118I RCCGGY 1 cut(s) 1055
Bse1I ACTGG 2 cut(s) 645, 1671
BseBI CCWGG 4 cut(s) 520, 550, 971, 1518
BseDI CCNNGG 1 cut(s) 1274
BseGI GGATG 4 cut(s) 374, 475, 1111, 1387
BseLI CCNNNNNNNGG 1 cut(s) 213
BseMII CTCAG 5 cut(s) 621, 752, 960, 1076, 1344
BseNI ACTGG 2 cut(s) 645, 1671
BseRI GAGGAG 4 cut(s) 230, 269, 374, 1388
BseSI GKGCMC 1 cut(s) 1157
BseXI GCAGC 7 cut(s) 17, 184, 688, 1282, 1537, 1554, 1672
BsgI GTGCAG 2 cut(s) 1632, 1679
BshFI GGCC 2 cut(s) 166, 1566
BsiHKAI GWGCWC 2 cut(s) 947, 1157
BsiSI CCGG 2 cut(s) 20, 1056
BsiWI CGTACG 1 cut(s) 704
BslI CCNNNNNNNGG 1 cut(s) 213
BsmAI GTCTC 4 cut(s) 635, 1232, 1387, 1454
BsmI GAATGC 3 cut(s) 481, 940, 1029
BsnI GGCC 2 cut(s) 166, 1566
Bso31I GGTCTC 1 cut(s) 635
Bsp1286I GDGCHC 2 cut(s) 947, 1157
Bsp143I GATC 5 cut(s) 297, 541, 784, 975, 1060
Bsp1720I GCTNAGC 1 cut(s) 946
Bsp19I CCATGG 1 cut(s) 1274
BspACI CCGC 2 cut(s) 208, 1283
BspANI GGCC 2 cut(s) 166, 1566
BspCNI CTCAG 5 cut(s) 622, 753, 959, 1077, 1345
BspHI TCATGA 2 cut(s) 118, 1140
BspPI GGATC 2 cut(s) 792, 1055
BspTNI GGTCTC 1 cut(s) 635
BsrFI RCCGGY 1 cut(s) 1055
BsrI ACTGG 2 cut(s) 645, 1671
BssAI RCCGGY 1 cut(s) 1055
BssECI CCNNGG 1 cut(s) 1274
BssMI GATC 5 cut(s) 297, 541, 784, 975, 1060
BssNAI GTATAC 1 cut(s) 772
BssT1I CCWWGG 1 cut(s) 1274
Bst1107I GTATAC 1 cut(s) 772
Bst2UI CCWGG 4 cut(s) 520, 550, 971, 1518
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 3 cut(s) 527, 1302, 1407
BstBAI YACGTR 1 cut(s) 708
BstC8I GCNNGC 4 cut(s) 1053, 1176, 1365, 1712
BstDEI CTNAG 6 cut(s) 630, 761, 946, 1085, 1353, 1652
BstDSI CCRYGG 1 cut(s) 1274
BstF5I GGATG 4 cut(s) 374, 475, 1111, 1387
BstKTI GATC 5 cut(s) 300, 544, 787, 978, 1063
BstMAI GTCTC 4 cut(s) 635, 1232, 1387, 1454
BstMBI GATC 5 cut(s) 297, 541, 784, 975, 1060
BstMWI GCNNNNNNNGC 2 cut(s) 463, 1364
BstNI CCWGG 4 cut(s) 520, 550, 971, 1518
BstNSI RCATGY 2 cut(s) 1178, 1731
BstSCI CCNGG 4 cut(s) 518, 548, 969, 1516
BstSLI GKGCMC 1 cut(s) 1157
BstV1I GCAGC 7 cut(s) 17, 184, 688, 1282, 1537, 1554, 1672
BstX2I RGATCY 2 cut(s) 784, 975
BstXI CCANNNNNNTGG 1 cut(s) 1025
BstYI RGATCY 2 cut(s) 784, 975
BstZ17I GTATAC 1 cut(s) 772
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 2 cut(s) 166, 1566
BtgI CCRYGG 1 cut(s) 1274
BtgZI GCGATG 1 cut(s) 1403
BtsCI GGATG 4 cut(s) 374, 475, 1111, 1387
BtsIMutI CAGTG 2 cut(s) 852, 1664
Cac8I GCNNGC 4 cut(s) 1053, 1176, 1365, 1712
CaiI CAGNNNCTG 2 cut(s) 854, 1666
CciI TCATGA 2 cut(s) 118, 1140
Cfr10I RCCGGY 1 cut(s) 1055
Csp6I GTAC 2 cut(s) 705, 872
CviQI GTAC 2 cut(s) 705, 872
DdeI CTNAG 6 cut(s) 630, 761, 946, 1085, 1353, 1652
DpnI GATC 5 cut(s) 299, 543, 786, 977, 1062
DpnII GATC 5 cut(s) 297, 541, 784, 975, 1060
DraI TTTAAA 1 cut(s) 444
DriI GACNNNNNGTC 1 cut(s) 1467
Eam1104I CTCTTC 3 cut(s) 527, 1302, 1407
Eam1105I GACNNNNNGTC 1 cut(s) 1467
EarI CTCTTC 3 cut(s) 527, 1302, 1407
Ecl136II GAGCTC 1 cut(s) 945
Eco130I CCWWGG 1 cut(s) 1274
Eco24I GRGCYC 1 cut(s) 947
Eco31I GGTCTC 1 cut(s) 635
Eco32I GATATC 1 cut(s) 1015
Eco53kI GAGCTC 1 cut(s) 945
Eco57I CTGAAG 2 cut(s) 267, 453
EcoICRI GAGCTC 1 cut(s) 945
EcoRII CCWGG 4 cut(s) 518, 548, 969, 1516
EcoRV GATATC 1 cut(s) 1015
EcoT14I CCWWGG 1 cut(s) 1274
EcoT22I ATGCAT 1 cut(s) 940
EcoT38I GRGCYC 1 cut(s) 947
ErhI CCWWGG 1 cut(s) 1274
FalI AAGNNNNNCTT 2 cut(s) 1278, 1310
FauI CCCGC 1 cut(s) 215
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 7 cut(s) 31, 198, 677, 1271, 1543, 1551, 1661
FokI GGATG 4 cut(s) 381, 482, 1098, 1394
FriOI GRGCYC 1 cut(s) 947
Fsp4HI GCNGC 7 cut(s) 31, 198, 677, 1271, 1543, 1551, 1661
FspBI CTAG 5 cut(s) 191, 452, 509, 1266, 1620
GluI GCNGC 7 cut(s) 31, 198, 677, 1271, 1543, 1551, 1661
GsuI CTGGAG 1 cut(s) 1539
HaeIII GGCC 2 cut(s) 166, 1566
HapII CCGG 2 cut(s) 20, 1056
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 1 cut(s) 1480
HpaI GTTAAC 1 cut(s) 691
HpaII CCGG 2 cut(s) 20, 1056
HphI GGTGA 3 cut(s) 805, 1070, 1207
Hpy166II GTNNAC 4 cut(s) 428, 691, 772, 1155
Hpy188III TCNNGA 5 cut(s) 75, 119, 318, 1141, 1325
Hpy8I GTNNAC 4 cut(s) 428, 691, 772, 1155
HpyAV CCTTC 5 cut(s) 149, 734, 775, 1063, 1306
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 1 cut(s) 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 463, 1364
HpyF3I CTNAG 6 cut(s) 630, 761, 946, 1085, 1353, 1652
HpySE526I ACGT 1 cut(s) 707
Ksp22I TGATCA 1 cut(s) 297
KspAI GTTAAC 1 cut(s) 691
Kzo9I GATC 5 cut(s) 297, 541, 784, 975, 1060
LmnI GCTCC 3 cut(s) 243, 942, 1171
Lsp1109I GCAGC 7 cut(s) 17, 184, 688, 1282, 1537, 1554, 1672
LweI GCATC 5 cut(s) 322, 583, 663, 745, 1120
MaeI CTAG 5 cut(s) 191, 452, 509, 1266, 1620
MaeII ACGT 1 cut(s) 707
MaeIII GTNAC 6 cut(s) 524, 598, 743, 1077, 1615, 1633
MalI GATC 5 cut(s) 299, 543, 786, 977, 1062
MboI GATC 5 cut(s) 297, 541, 784, 975, 1060
MboII GAAGA 9 cut(s) 127, 164, 446, 544, 899, 1120, 1298, 1319, 1424
MfeI CAATTG 1 cut(s) 1338
MflI RGATCY 2 cut(s) 784, 975
MhlI GDGCHC 2 cut(s) 947, 1157
MluCI AATT 9 cut(s) 94, 99, 142, 228, 417, 446, 890, 1338, 1464
MlyI GAGTC 4 cut(s) 1085, 1234, 1389, 1415
MmeI TCCRAC 2 cut(s) 648, 1451
Mph1103I ATGCAT 1 cut(s) 940
MseI TTAA 6 cut(s) 98, 393, 443, 690, 1376, 1671
MslI CAYNNNNRTG 2 cut(s) 929, 1116
MspI CCGG 2 cut(s) 20, 1056
MspR9I CCNGG 4 cut(s) 520, 550, 971, 1518
MunI CAATTG 1 cut(s) 1338
Mva1269I GAATGC 3 cut(s) 481, 940, 1029
MvaI CCWGG 4 cut(s) 520, 550, 971, 1518
MwoI GCNNNNNNNGC 2 cut(s) 463, 1364
NcoI CCATGG 1 cut(s) 1274
NdeII GATC 5 cut(s) 297, 541, 784, 975, 1060
NmuCI GTSAC 2 cut(s) 598, 1077
NsiI ATGCAT 1 cut(s) 940
NspI RCATGY 2 cut(s) 1178, 1731
PaeI GCATGC 1 cut(s) 1178
PagI TCATGA 2 cut(s) 118, 1140
PciI ACATGT 1 cut(s) 1727
PcsI WCGNNNNNNNCGW 1 cut(s) 67
PctI GAATGC 3 cut(s) 481, 940, 1029
PfeI GAWTC 7 cut(s) 275, 314, 437, 796, 1212, 1418, 1584
Pfl23II CGTACG 1 cut(s) 704
PkrI GCNGC 7 cut(s) 32, 199, 678, 1272, 1544, 1552, 1662
PleI GAGTC 4 cut(s) 1084, 1234, 1389, 1414
PpsI GAGTC 4 cut(s) 1084, 1234, 1389, 1414
Ppu21I YACGTR 1 cut(s) 708
PscI ACATGT 1 cut(s) 1727
PsiI TTATAA 1 cut(s) 176
Psp124BI GAGCTC 1 cut(s) 947
Psp6I CCWGG 4 cut(s) 518, 548, 969, 1516
PspGI CCWGG 4 cut(s) 518, 548, 969, 1516
PspLI CGTACG 1 cut(s) 704
PstNI CAGNNNCTG 2 cut(s) 854, 1666
PsuI RGATCY 2 cut(s) 784, 975
RsaI GTAC 2 cut(s) 706, 873
RsaNI GTAC 2 cut(s) 705, 872
RseI CAYNNNNRTG 2 cut(s) 929, 1116
SacI GAGCTC 1 cut(s) 947
SaqAI TTAA 6 cut(s) 98, 393, 443, 690, 1376, 1671
SatI GCNGC 7 cut(s) 31, 198, 677, 1271, 1543, 1551, 1661
Sau3AI GATC 5 cut(s) 297, 541, 784, 975, 1060
SchI GAGTC 4 cut(s) 1085, 1234, 1389, 1415
ScrFI CCNGG 4 cut(s) 520, 550, 971, 1518
SduI GDGCHC 2 cut(s) 947, 1157
SfaNI GCATC 5 cut(s) 322, 583, 663, 745, 1120
SmiMI CAYNNNNRTG 2 cut(s) 929, 1116
SmlI CTYRAG 1 cut(s) 1483
SmoI CTYRAG 1 cut(s) 1483
SphI GCATGC 1 cut(s) 1178
Sse9I AATT 9 cut(s) 94, 99, 142, 228, 417, 446, 890, 1338, 1464
SsiI CCGC 2 cut(s) 208, 1283
SspI AATATT 1 cut(s) 505
SspMI CTAG 5 cut(s) 191, 452, 509, 1266, 1620
SstI GAGCTC 1 cut(s) 947
StyD4I CCNGG 4 cut(s) 518, 548, 969, 1516
StyI CCWWGG 1 cut(s) 1274
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 1 cut(s) 710
TaqI TCGA 3 cut(s) 61, 74, 1587
TasI AATT 9 cut(s) 94, 99, 142, 228, 417, 446, 890, 1338, 1464
TfiI GAWTC 7 cut(s) 275, 314, 437, 796, 1212, 1418, 1584
Tru1I TTAA 6 cut(s) 98, 393, 443, 690, 1376, 1671
Tru9I TTAA 6 cut(s) 98, 393, 443, 690, 1376, 1671
TscAI CASTG 2 cut(s) 859, 1671
TseFI GTSAC 2 cut(s) 598, 1077
TseI GCWGC 7 cut(s) 30, 197, 676, 1270, 1542, 1550, 1660
Tsp45I GTSAC 2 cut(s) 598, 1077
TspRI CASTG 2 cut(s) 859, 1671
VneI GTGCAC 1 cut(s) 1153
XapI RAATTY 1 cut(s) 890
XceI RCATGY 2 cut(s) 1178, 1731
XmiI GTMKAC 1 cut(s) 771
XspI CTAG 5 cut(s) 191, 452, 509, 1266, 1620
Zsp2I ATGCAT 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.