Rorug02G0005700

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006901
Physical Location & Seq
Reverse (-)
553780 .. 562861
9082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0005700.1

Sequence Viewer

Length: 4917 bp
ATGGAATCACCTGTTGGAGGTGTGAGGCAAACATCTGTGATAGTTGTTACATTGGAAACTGGTGAAGTATATGTGATTGCGAGTTTGTCTTCTAGGCTCGACACTCAGGTCATATATGTTGATCCTACTACTGGAGCACTTCGCTACAATGCGAAGCCAGGATTTGATGTTTTTAAATCCGAAAAGGAAGCTTTGAATTACATTACAAACGGTTCGGAGTGGTTGTGTAGGAGCACGACCTATGCCCGTGCAATATTGGGATATGCTGCTTTGGGCAGCTTTGGTTTGCTTCTTGTGGCTACCAAGTTGACTGCTACTGTTCCGAATCTGCCTGGCGGAGGGTCTGTGTATACAGTGACTGAGAGTCAATGGATCAAGATATTGCTTCAGAATCCACAGCCCCAGGGGAAAGGAGAAGTGAAGAATGTTAATGAATTGACTGATATGGACATTGATGGGAAGCATTACTTTTGTGAGGCAAGGGACATCACTCGGCCATTTCCTAGCCGTATGTGCTTGCGTGAACCTGACGATGAATTTGTTTGGAATGCTTGGTTCTCGATGCCTTTCAAAAACATTGGGCTTCCGCACCATTGTGTCACTCTTCTTCAGGGTTTTGCAGAATGTCGAGGTTTTGGATCCTCAGGGAATCTGGAAGGAGTTGTTGCTCTCATAGCTCGTCGTAGCAGGCTGCATCCTGGTACTCGATACTTGGCTAGGGGATTAAATTCATGTTATAGCACAGGAAACGAAGTGGAGTGTGAGCAACTTGTATGGGTCCCTAGAAGGGCTGGCCAAACTGTTCCTTTCAACACGTATGTCTGGCGGCGGGGGACAATTCCAATCTGGTGGGGTGCAGAGTTAAAGATCACTGCTGCCGAAGCAGAAATATATGTTTCAGACCGCGATCCTTATAAAGGGAGTGCAGATTACTACCAGAGGTTGAGTAAGAGGTATGATGCACGGAATTTAGATGTAGCTGTTGGGGGGACTCAGAATCGAAAAGCATCAGTTCCAATTGTATGTATCAACTTGCTTAGAAATGGAGAAGGAAAGTCGGAATCCATTTTAGTACAGCATTTTGAGGAATCTTTAAACTACATCAGATCCACAGGAAAACTTCCTTATACGCGAATTCCTTTAATAAATTATGACTGGCATGCAAGTATAAAGTTAAAAGGCGAACAGCAAACCATTGAAGGATTATGGAGACATCTAAAAGCACCCACTGTATCTATAGCCATTTCTGAAGGAGATTATTTGCCTTCACGAGACAGAATTAAGGACTGCAGAGGAGAAATTATCTACAATGATGACTTTGAAGGTGCTTTCTGCTTGAGATCCCATCAAAATGGTGTAATACGTTTCAACTGTGCTGATTCTTTGGATAGAACAAATGCTGCAAGTTATTTTGGCTCTCTCCAGGTTTTTGTGGAGCAGTGTAGGCGGCTTGGTATATCGCTTGACAGTGATTTGGCATTTGGTTATCCGTCAATGACTAATTATGCCGGATATACAGCTCCTTTGCCACCAGGCTGGGAGAAGAGGTCGGATGCAGTAACAGGGAAAACATACTACATTGATCACAATACTAGGACCACAACATGGATGCATCCATGTCCCGATAAACCTTGGAAAAGATTTGATATGTCATTTGAGGAATTCAAGAGGTCAACAATTTTATCTCCAGTATCCACGCTTGCAGATCTTTTTCTGCTCGCTGGTGATATTCATGCAACACTTTATACGGGCTCTAAAGCCATGCATAGTCAAATCCTCAGCATTTTCAATGAAGATGCTGGAAAGTTTAAACAATTTTCTGCAGCACAGAATATGAAAATAACTTTGCAGAGACGGTATAAAAATGCAGTTGTAGACAGCTCTCGTCAAAAGCAACTGGAAATGTTCCTTGGAATGAGATTATTCAAGCATCTTCCATCAGTTTCTTTTCACCCTCTAAATGTAGTCTCTCGACCATCTGGTTTCTTCCTTAAGCCAGTTGCTAACATGTTTCCAAGTTCCAATGGTGAAGCCAGTCTTCTGAGTTTCAAAAGAAAAGACCTAATATGGGTTTGCCCACAAGCTGCAGATGTGGTTGAACTTTTTATCTATCTAGGTGAGCCTTGCCATGTATGTCAGCTTCTTCTCACAGTATCCCATGGCGCAGATGACTCGACCTATCCATCAACAGTTGATGTAAGGACGGGCCGCTGTTTAGATGGGCTAAAACTGGTCTTGGAGGGTGCTTCGATCCCTCACTGTGTAAATGGAACAAACCTTGTGATACCCATACCGGGGCCAATTAGTGCAGAGGATATGGCTATAACAGGAGCTGGTGCACGCCTTCATGCTCAAGATATATCTACCCTTCCATTATTGTATGATTTTGAAGAATTGGAAGGAGAACTTGACTTCCTTACTCGTGTAGTTGCCCTTACATTTTATCCCGCTGTCTCTGGAAGAACCCCTATCACTCTTGGTGAGATAGAAGTCCTTGGAGTTTCTCTTCCTTGGAAGGGTGTATTTAATAAAGAAGGCCCTGGTGCAAGATTACCTGAACAGGCTAAAAACTTTCAGAATGAAACCAAACCATCTTTTTCTCGTTCAGATACCAATCCATTTTCTGGTGCTTCATCTAATGATGTGCCACCACCAGTGCAACCAAGTGTGTCCGAAGACAATTTGGTTGACCTGTTGACTGGAGAGGTCATCCTTTCAGAACAGATTGCTCAGCCAGTGATAGGAAATGCTGTGGATAAGGGAGGTGACTTGCTTGATTTTTTAGACCAAGCTGTTGTTGAATATCATGGTGCTCAAAATGATCATAAATTGTCTTCATCACACGATGGTAGATCTTCAGATAGCAGTTCCCAGCAGTACGTTGATCGATTGAAATCACTTACTGGGCCACGTATGGAAAGAAAACTAGACTTCATGGAAGCTATGAAACTTGAAATTGAGCGACTCCGGCTGAATATTTCTGCTGCTGAAAGGGATAGAGCTTTATTATCAATTGGAACTGATCCTGCTACCATAAATCCTAATGTGTTACTTGATGAACGTTACATGGGAAGGTTGTGCAGAGTTGCAAACTCCCTTGCTCTTCTTGGACAAGTGTCCTTGGAAGACAGAATTACATCTGCTATTGGTCTTGAGACTACTGATGACAATGTGATAGATTTTTGGAATATAAGTAAAATTGGGGAGTGTTGTTATGGGGGCATGTGTGAGGTGCGTGCTGAAACTGATCCACGTACAAGTAAATCCTTTTCAGAACCATCAGGTGGAGGTTCTCCTTCGATCTTGTTATGTTCTCAATGTCAAAGGAAAGTTTGTAAAGTTTGTTGTGCTGGGAGAGGAGCCCTTCTGGTTTCAGGCTATGGCTCAAGGGAGGCCACAAATTACAATGGTGTAGTAAGGCAGGGGGGATCAAGCCATGGTAGCCAGGTTGATATCTCTACAAATCGTTCAGTCGTGCTTGATGGTGTTGTTTGCAAACGATGCTGCCATGAGATTGTGCTTTTTGCTTTGATATTGGACTACGTTAGGGTTTTGTTAAGCATGAGGAGAAGTTCCCGTGCAGATGCTTCTGCGCATGAAGCCTTGAACCAGGTGACTGGATTTTCCTTAAAAGATGGTCTTTCTGAGAGTAATCAGTCTTCTGGAAAGAGATCCATTAAATCTCTGCGACAAGTACTTGACGGAGAGGAATCCCTTGCTGAGTTTCCATTCGCCAGCTTCTTACACTCGGTTGAAACGGCTACTGATTCAGCACCACTCTTGTCATTGCTTGCTCCTCTTGATTGTGGATCACGGCATTCTTACTGGAAAGCTCCTCCTAGTACAACCTCTGTTGAATTCATTATTGTTCTTGGTACCCTTTCTGATGTCAGTGGGGTTTCTTTGCTTATTAGTCCATGTGGTTACTCCGAGGCTGAAGCTCCCACCGTGCAAATCTGGGCCAGCAATAAAATACACAAGGAAGAAAGGTCATGCATGGGAAAATGGGATGTGCAGTCCCTGATCGCGTCTTCCTCAGAATATTTTGGACCAGAAAAATTGGTTAGAGAAGATCAGGTGCCTAGGCATGTGAAGTTTGCATTCAAGAATCCAGTTCGGTGTCGCATTATTTGGGTAACCTTACGTCTTCAACGACCTGGTTCAAGTTCTCTTAATTTTGAGAACTTGAATCTGTTGTCTCTTGATGAAAATCCATTTGCAGAAGTAACTCGGCGTGCCTCTTTCGGAGGAGCAGTTGAAAGAGAGCCCTGTCTTCATGCAAAAAGGATACTGGTAGTAGGAAGCCCAGTGAAAAAAGATCTGGCACGCACATCTTCACAAGGCTCTGATCAGATGAATATGAAAAACTGGGTGGAGAGAGATCCACAATTAAATAGATTTAGGGTTCCAATTGAGGCTGAGAGGTTGTTGGATAATGATATAGTCTTGGAACAGTTTCTATCTCCTGCTTCACCTTTGCTTGCTGGATTTCGTCTTGATGCTTTTGGTGCAATAAAACCTTTGGTTACCCATTCGCCGCCTTCAAATGCACGCATTTGGGATGTGTCAGCCACACTTTTAGATGACAGGCACATCTCTCCTGCTGTGCTGTATATACAAGTATCTATTTTCCAGGAACCCAACAACACGGTGACAGTAGCAGAATATAGGTTGCCAGAGGCTAAGCCTGGAACTGCTATGTACTTTGATTTCCCTAGAAAGATACAAACCCGCAGAATTACATTTAAACTTCTTGGAGATGTTACAGCATTTGCAGATGATCCAACAGAACAGGATGATCCAGGTTCCAGAGGTCTACCAGTAGCAGCCGGATTGTCATTAGCCAATAGAATTAAGTTGTATTACTATGATGATCCCTACGAACTTGGAAAATGGGCGAGCCTTTCAGCAGTTTGA

Protein Analysis

1638

Amino Acids

181.17

Weight (kDa)

6.23

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syja_N PF02383 84 - 390 5.1e-39 SacI homology domain
WW PF00397 509 - 539 1.1e-11 WW domain
SAC9_GBDL_1st PF24790 653 - 838 4.1e-98 SAC9 first GBDL domain
SAC9_C8D PF24791 1066 - 1175 2.7e-20 SAC9 C8D domain
SAC9_GBDL_2nd PF24789 1236 - 1435 5.1e-101 SAC9 second GBDL domain
SAC9_C PF24765 1463 - 1638 1.1e-89 SAC9 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
AasI GACNNNNNNGTC 1 cut(s) 107
Acc16I TGCGCA 1 cut(s) 3594
Acc65I GGTACC 1 cut(s) 3875
AccB1I GGYRCC 2 cut(s) 3875, 4078
AccB7I CCANNNNNTGG 3 cut(s) 1605, 2624, 3284
AccI GTMKAC 3 cut(s) 350, 1875, 4816
AccII CGCG 3 cut(s) 906, 1132, 4028
AclI AACGTT 1 cut(s) 3061
AcoI YGGCCR 2 cut(s) 494, 793
AcsI RAATTY 6 cut(s) 536, 727, 967, 1134, 1661, 3857
AcuI CTGAAG 5 cut(s) 371, 593, 1269, 2841, 3957
AfaI GTAC 8 cut(s) 703, 1074, 2879, 3256, 3696, 3844, 3877, 4703
AflII CTTAAG 1 cut(s) 1991
AflIII ACRYGT 2 cut(s) 813, 2007
AhdI GACNNNNNGTC 1 cut(s) 363
AjuI GAANNNNNNNTTGG 3 cut(s) 29, 1893, 1925
AleI CACNNNNGTG 1 cut(s) 594
AloI GAACNNNNNNTCC 2 cut(s) 2397, 2429
Alw21I GWGCWC 4 cut(s) 139, 236, 2341, 2815
Alw26I GTCTC 7 cut(s) 1204, 1266, 1846, 1972, 2458, 3149, 4203
Alw44I GTGCAC 1 cut(s) 2337
AlwNI CAGNNNCTG 3 cut(s) 359, 2333, 4021
AoxI GGCC 8 cut(s) 494, 793, 2206, 2297, 2536, 2906, 3393, 3960
ApaLI GTGCAC 1 cut(s) 2337
ApoI RAATTY 6 cut(s) 536, 727, 967, 1134, 1661, 3857
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
Asp700I GAANNNNTTC 1 cut(s) 4455
Asp718I GGTACC 1 cut(s) 3875
AspA2I CCTAGG 1 cut(s) 4082
AspLEI GCGC 2 cut(s) 2165, 3595
AspS9I GGNCC 8 cut(s) 778, 1596, 2206, 2297, 2537, 2906, 3960, 4049
AsuC2I CCSGG 1 cut(s) 2295
AvaII GGWCC 3 cut(s) 778, 1596, 4049
AvrII CCTAGG 1 cut(s) 4082
AxyI CCTNAGG 1 cut(s) 643
BaeGI GKGCMC 1 cut(s) 2341
BalI TGGCCA 1 cut(s) 795
BamHI GGATCC 1 cut(s) 638
BanI GGYRCC 2 cut(s) 3875, 4078
BanII GRGCYC 3 cut(s) 1754, 3364, 4269
BarI GAAGNNNNNNTAC 4 cut(s) 2124, 2156, 3643, 3675
BauI CACGAG 2 cut(s) 1269, 2421
BbsI GAAGAC 9 cut(s) 81, 2030, 2682, 2826, 3132, 3651, 4023, 4139, 4265
Bbv12I GWGCWC 4 cut(s) 139, 236, 2341, 2815
BbvCI CCTCAGC 1 cut(s) 1778
BceAI ACGGC 3 cut(s) 492, 3774, 3830
BcgI CGANNNNNNTGC 2 cut(s) 2154, 2188
BciVI GTATCC 3 cut(s) 1702, 2164, 4281
BclI TGATCA 3 cut(s) 1582, 2821, 4348
BcnI CCSGG 1 cut(s) 2295
BcoDI GTCTC 7 cut(s) 1204, 1266, 1846, 1972, 2458, 3149, 4203
BfmI CTRYAG 4 cut(s) 1236, 1288, 1821, 2085
BfrI CTTAAG 1 cut(s) 1991
BfuI GTATCC 3 cut(s) 1702, 2164, 4281
BglII AGATCT 3 cut(s) 1705, 2852, 4318
BlnI CCTAGG 1 cut(s) 4082
BlpI GCTNAGC 2 cut(s) 2730, 4683
BmcAI AGTACT 1 cut(s) 3696
Bme18I GGWCC 3 cut(s) 778, 1596, 4049
BmeRI GACNNNNNGTC 1 cut(s) 363
BmgT120I GGNCC 8 cut(s) 778, 1596, 2206, 2297, 2537, 2906, 3960, 4049
BmrI ACTGGG 3 cut(s) 2913, 4301, 4378
BmuI ACTGGG 3 cut(s) 2913, 4301, 4378
BoxI GACNNNNGTC 1 cut(s) 3115
BpiI GAAGAC 9 cut(s) 81, 2030, 2682, 2826, 3132, 3651, 4023, 4139, 4265
BpmI CTGGAG 4 cut(s) 153, 1406, 1671, 2721
Bpu10I CCTNAGC 1 cut(s) 1778
Bpu1102I GCTNAGC 2 cut(s) 2730, 4683
BpuEI CTTGAG 4 cut(s) 1357, 2337, 3173, 3370
BpuMI CCSGG 1 cut(s) 2295
Bsa29I ATCGAT 1 cut(s) 2887
BsaAI YACGTR 3 cut(s) 816, 2912, 3254
BsaBI GATNNNNATC 3 cut(s) 2613, 2893, 4209
BsaXI ACNNNNNCTCC 8 cut(s) 209, 239, 330, 360, 1532, 1562, 4239, 4269
Bse21I CCTNAGG 1 cut(s) 643
Bse3DI GCAATG 1 cut(s) 3785
Bse8I GATNNNNATC 3 cut(s) 2613, 2893, 4209
BseCI ATCGAT 1 cut(s) 2887
BseGI GGATG 8 cut(s) 694, 1558, 1612, 1614, 2709, 4015, 4567, 4801
BseJI GATNNNNATC 3 cut(s) 2613, 2893, 4209
BseMI GCAATG 1 cut(s) 3785
BseRI GAGGAG 6 cut(s) 1308, 3372, 3580, 3786, 3825, 4263
BseSI GKGCMC 1 cut(s) 2341
BseYI CCCAGC 3 cut(s) 1536, 2871, 3350
BsgI GTGCAG 6 cut(s) 876, 945, 2328, 3100, 3600, 4034
Bsh1236I CGCG 3 cut(s) 906, 1132, 4028
BshFI GGCC 8 cut(s) 496, 795, 2208, 2299, 2538, 2908, 3395, 3962
BshNI GGYRCC 2 cut(s) 3875, 4078
BshVI ATCGAT 1 cut(s) 2887
BsiHKAI GWGCWC 4 cut(s) 139, 236, 2341, 2815
BsiSI CCGG 4 cut(s) 1509, 2294, 2968, 4830
BslFI GGGAC 6 cut(s) 497, 764, 847, 1003, 1605, 4003
BsmAI GTCTC 7 cut(s) 1204, 1266, 1846, 1972, 2458, 3149, 4203
BsmBI CGTCTC 1 cut(s) 1846
BsmFI GGGAC 6 cut(s) 497, 764, 847, 1003, 1605, 4003
BsmI GAATGC 3 cut(s) 553, 3817, 4100
BsnI GGCC 8 cut(s) 496, 795, 2208, 2299, 2538, 2908, 3395, 3962
Bsp1286I GDGCHC 7 cut(s) 139, 236, 1754, 2341, 2815, 3364, 4269
Bsp1720I GCTNAGC 2 cut(s) 2730, 4683
Bsp19I CCATGG 2 cut(s) 2158, 3436
BspANI GGCC 8 cut(s) 496, 795, 2208, 2299, 2538, 2908, 3395, 3962
BspDI ATCGAT 1 cut(s) 2887
BspFNI CGCG 3 cut(s) 906, 1132, 4028
BspMAI CTGCAG 3 cut(s) 1292, 1825, 2089
BspQI GCTCTTC 1 cut(s) 3108
BspT107I GGYRCC 2 cut(s) 3875, 4078
BspTI CTTAAG 1 cut(s) 1991
BsrDI GCAATG 1 cut(s) 3785
BssNAI GTATAC 1 cut(s) 351
BssSI CACGAG 2 cut(s) 1269, 2421
BssT1I CCWWGG 8 cut(s) 1631, 1909, 2158, 2494, 2510, 3120, 3436, 4082
Bst1107I GTATAC 1 cut(s) 351
Bst2BI CACGAG 2 cut(s) 1269, 2421
Bst6I CTCTTC 4 cut(s) 609, 1538, 2511, 3108
BstAFI CTTAAG 1 cut(s) 1991
BstAPI GCANNNNNTGC 1 cut(s) 3501
BstBAI YACGTR 3 cut(s) 816, 2912, 3254
BstDSI CCRYGG 2 cut(s) 2158, 3436
BstEII GGTNACC 2 cut(s) 4135, 4525
BstENI CCTNNNNNAGG 3 cut(s) 15, 336, 915
BstF5I GGATG 8 cut(s) 694, 1558, 1612, 1614, 2709, 4015, 4567, 4801
BstFNI CGCG 3 cut(s) 906, 1132, 4028
BstHHI GCGC 2 cut(s) 2165, 3595
BstMAI GTCTC 7 cut(s) 1204, 1266, 1846, 1972, 2458, 3149, 4203
BstMWI GCNNNNNNNGC 9 cut(s) 513, 674, 881, 1444, 2968, 3441, 3501, 3599, 4508
BstNSI RCATGY 4 cut(s) 1163, 2011, 3226, 4091
BstPAI GACNNNNGTC 1 cut(s) 3115
BstPI GGTNACC 2 cut(s) 4135, 4525
BstSFI CTRYAG 4 cut(s) 1236, 1288, 1821, 2085
BstSLI GKGCMC 1 cut(s) 2341
BstUI CGCG 3 cut(s) 906, 1132, 4028
BstV2I GAAGAC 9 cut(s) 81, 2030, 2682, 2826, 3132, 3651, 4023, 4139, 4265
BstX2I RGATCY 8 cut(s) 638, 1106, 1340, 1705, 2852, 3671, 4318, 4381
BstXI CCANNNNNNTGG 4 cut(s) 849, 1352, 1536, 3617
BstYI RGATCY 8 cut(s) 638, 1106, 1340, 1705, 2852, 3671, 4318, 4381
BstZ17I GTATAC 1 cut(s) 351
Bsu15I ATCGAT 1 cut(s) 2887
Bsu36I CCTNAGG 1 cut(s) 643
BsuI GTATCC 3 cut(s) 1702, 2164, 4281
BsuRI GGCC 8 cut(s) 496, 795, 2208, 2299, 2538, 2908, 3395, 3962
BsuTUI ATCGAT 1 cut(s) 2887
BtgI CCRYGG 2 cut(s) 2158, 3436
BtsCI GGATG 8 cut(s) 694, 1558, 1612, 1614, 2709, 4015, 4567, 4801
BtsI GCAGTG 2 cut(s) 870, 1445
CaiI CAGNNNCTG 3 cut(s) 359, 2333, 4021
CfoI GCGC 2 cut(s) 2165, 3595
Cfr13I GGNCC 8 cut(s) 778, 1596, 2206, 2297, 2537, 2906, 3960, 4049
ClaI ATCGAT 1 cut(s) 2887
CseI GACGC 1 cut(s) 4017
CsiI ACCWGGT 2 cut(s) 3609, 4156
Csp6I GTAC 8 cut(s) 702, 1073, 2878, 3255, 3695, 3843, 3876, 4702
CviQI GTAC 8 cut(s) 702, 1073, 2878, 3255, 3695, 3843, 3876, 4702
DraI TTTAAA 4 cut(s) 175, 1095, 1810, 4747
DrdI GACNNNNNNGTC 1 cut(s) 107
DriI GACNNNNNGTC 1 cut(s) 363
DseDI GACNNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 2 cut(s) 494, 793
Eam1104I CTCTTC 4 cut(s) 609, 1538, 2511, 3108
Eam1105I GACNNNNNGTC 1 cut(s) 363
EarI CTCTTC 4 cut(s) 609, 1538, 2511, 3108
EciI GGCGGA 1 cut(s) 351
Eco130I CCWWGG 8 cut(s) 1631, 1909, 2158, 2494, 2510, 3120, 3436, 4082
Eco24I GRGCYC 3 cut(s) 1754, 3364, 4269
Eco32I GATATC 1 cut(s) 3454
Eco47I GGWCC 3 cut(s) 778, 1596, 4049
Eco57I CTGAAG 5 cut(s) 371, 593, 1269, 2841, 3957
Eco81I CCTNAGG 1 cut(s) 643
Eco91I GGTNACC 2 cut(s) 4135, 4525
EcoNI CCTNNNNNAGG 3 cut(s) 15, 336, 915
EcoO109I RGGNCCY 2 cut(s) 778, 2537
EcoO65I GGTNACC 2 cut(s) 4135, 4525
EcoRI GAATTC 3 cut(s) 1134, 1661, 3857
EcoRV GATATC 1 cut(s) 3454
EcoT14I CCWWGG 8 cut(s) 1631, 1909, 2158, 2494, 2510, 3120, 3436, 4082
EcoT22I ATGCAT 3 cut(s) 1614, 1767, 3998
EcoT38I GRGCYC 3 cut(s) 1754, 3364, 4269
ErhI CCWWGG 8 cut(s) 1631, 1909, 2158, 2494, 2510, 3120, 3436, 4082
Esp3I CGTCTC 1 cut(s) 1846
FalI AAGNNNNNCTT 8 cut(s) 452, 484, 2022, 2054, 2391, 2423, 3624, 3656
FaqI GGGAC 6 cut(s) 497, 764, 847, 1003, 1605, 4003
FauI CCCGC 3 cut(s) 822, 2455, 4739
FbaI TGATCA 3 cut(s) 1582, 2821, 4348
FblI GTMKAC 3 cut(s) 350, 1875, 4816
FokI GGATG 8 cut(s) 681, 1565, 1599, 1621, 2696, 4022, 4574, 4808
FriOI GRGCYC 3 cut(s) 1754, 3364, 4269
FspI TGCGCA 1 cut(s) 3594
GlaI GCGC 2 cut(s) 2164, 3594
GsaI CCCAGC 3 cut(s) 1540, 2875, 3354
GsuI CTGGAG 4 cut(s) 153, 1406, 1671, 2721
HaeIII GGCC 8 cut(s) 496, 795, 2208, 2299, 2538, 2908, 3395, 3962
HapII CCGG 4 cut(s) 1509, 2294, 2968, 4830
HgaI GACGC 1 cut(s) 4017
HhaI GCGC 2 cut(s) 2165, 3595
Hin6I GCGC 2 cut(s) 2163, 3593
HinP1I GCGC 2 cut(s) 2163, 3593
HincII GTYRAC 4 cut(s) 309, 1674, 2689, 2697
HindII GTYRAC 4 cut(s) 309, 1674, 2689, 2697
HindIII AAGCTT 1 cut(s) 189
HpaII CCGG 4 cut(s) 1509, 2294, 2968, 4830
Hpy166II GTNNAC 9 cut(s) 309, 351, 524, 1674, 1876, 2339, 2689, 2697, 4817
Hpy8I GTNNAC 9 cut(s) 309, 351, 524, 1674, 1876, 2339, 2689, 2697, 4817
Hpy99I CGWCG 1 cut(s) 684
HpyCH4IV ACGT 8 cut(s) 815, 1363, 2880, 2911, 3061, 3253, 3543, 4144
HpyF10VI GCNNNNNNNGC 9 cut(s) 513, 674, 881, 1444, 2968, 3441, 3501, 3599, 4508
HpySE526I ACGT 8 cut(s) 815, 1363, 2880, 2911, 3061, 3253, 3543, 4144
HspAI GCGC 2 cut(s) 2163, 3593
KflI GGGWCCC 1 cut(s) 778
KpnI GGTACC 1 cut(s) 3879
Ksp22I TGATCA 3 cut(s) 1582, 2821, 4348
LguI GCTCTTC 1 cut(s) 3108
MabI ACCWGGT 2 cut(s) 3609, 4156
MaeII ACGT 8 cut(s) 815, 1363, 2880, 2911, 3061, 3253, 3543, 4144
MfeI CAATTG 3 cut(s) 1017, 3012, 4410
MflI RGATCY 8 cut(s) 638, 1106, 1340, 1705, 2852, 3671, 4318, 4381
MhlI GDGCHC 7 cut(s) 139, 236, 1754, 2341, 2815, 3364, 4269
MlsI TGGCCA 1 cut(s) 795
MluNI TGGCCA 1 cut(s) 795
MlyI GAGTC 4 cut(s) 373, 985, 2165, 2958
MmeI TCCRAC 4 cut(s) 1038, 1530, 4410, 4808
Mox20I TGGCCA 1 cut(s) 795
Mph1103I ATGCAT 3 cut(s) 1614, 1767, 3998
MroXI GAANNNNTTC 1 cut(s) 4455
MscI TGGCCA 1 cut(s) 795
MslI CAYNNNNRTG 2 cut(s) 594, 1350
Msp20I TGGCCA 1 cut(s) 795
MspA1I CMGCKG 2 cut(s) 2211, 2450
MspCI CTTAAG 1 cut(s) 1991
MspI CCGG 4 cut(s) 1509, 2294, 2968, 4830
MssI GTTTAAAC 1 cut(s) 1810
MunI CAATTG 3 cut(s) 1017, 3012, 4410
Mva1269I GAATGC 3 cut(s) 553, 3817, 4100
MvnI CGCG 3 cut(s) 906, 1132, 4028
MwoI GCNNNNNNNGC 9 cut(s) 513, 674, 881, 1444, 2968, 3441, 3501, 3599, 4508
NciI CCSGG 1 cut(s) 2295
NcoI CCATGG 2 cut(s) 2158, 3436
NmeAIII GCCGAG 2 cut(s) 472, 4210
NmuCI GTSAC 5 cut(s) 355, 598, 2765, 3613, 4651
NsbI TGCGCA 1 cut(s) 3594
NsiI ATGCAT 3 cut(s) 1614, 1767, 3998
NspI RCATGY 4 cut(s) 1163, 2011, 3226, 4091
OliI CACNNNNGTG 1 cut(s) 594
PaeI GCATGC 1 cut(s) 1163
PasI CCCWGGG 1 cut(s) 403
PciI ACATGT 1 cut(s) 2007
PciSI GCTCTTC 1 cut(s) 3108
PcsI WCGNNNNNNNCGW 1 cut(s) 4150
PctI GAATGC 3 cut(s) 553, 3817, 4100
PdmI GAANNNNTTC 1 cut(s) 4455
PflMI CCANNNNNTGG 3 cut(s) 1605, 2624, 3284
PfoI TCCNGGA 1 cut(s) 4632
PleI GAGTC 4 cut(s) 372, 985, 2165, 2958
PmeI GTTTAAAC 1 cut(s) 1810
PpsI GAGTC 4 cut(s) 372, 985, 2165, 2958
Ppu21I YACGTR 3 cut(s) 816, 2912, 3254
PpuMI RGGWCCY 1 cut(s) 778
PscI ACATGT 1 cut(s) 2007
PshAI GACNNNNGTC 1 cut(s) 3115
PsiI TTATAA 1 cut(s) 915
Psp1406I AACGTT 1 cut(s) 3061
Psp5II RGGWCCY 1 cut(s) 778
PspEI GGTNACC 2 cut(s) 4135, 4525
PspFI CCCAGC 3 cut(s) 1536, 2871, 3350
PspPI GGNCC 8 cut(s) 778, 1596, 2206, 2297, 2537, 2906, 3960, 4049
PspPPI RGGWCCY 1 cut(s) 778
PstI CTGCAG 3 cut(s) 1292, 1825, 2089
PstNI CAGNNNCTG 3 cut(s) 359, 2333, 4021
PsuI RGATCY 8 cut(s) 638, 1106, 1340, 1705, 2852, 3671, 4318, 4381
RsaI GTAC 8 cut(s) 703, 1074, 2879, 3256, 3696, 3844, 3877, 4703
RsaNI GTAC 8 cut(s) 702, 1073, 2878, 3255, 3695, 3843, 3876, 4702
RseI CAYNNNNRTG 2 cut(s) 594, 1350
SapI GCTCTTC 1 cut(s) 3108
Sau96I GGNCC 8 cut(s) 778, 1596, 2206, 2297, 2537, 2906, 3960, 4049
ScaI AGTACT 1 cut(s) 3696
SchI GAGTC 4 cut(s) 373, 985, 2165, 2958
SduI GDGCHC 7 cut(s) 139, 236, 1754, 2341, 2815, 3364, 4269
SexAI ACCWGGT 2 cut(s) 3609, 4156
SfcI CTRYAG 4 cut(s) 1236, 1288, 1821, 2085
SinI GGWCC 3 cut(s) 778, 1596, 4049
SmiMI CAYNNNNRTG 2 cut(s) 594, 1350
SmlI CTYRAG 5 cut(s) 1336, 1991, 2352, 3152, 3385
SmoI CTYRAG 5 cut(s) 1336, 1991, 2352, 3152, 3385
SphI GCATGC 1 cut(s) 1163
SspI AATATT 3 cut(s) 255, 2977, 4043
StyI CCWWGG 8 cut(s) 1631, 1909, 2158, 2494, 2510, 3120, 3436, 4082
TaiI ACGT 8 cut(s) 818, 1366, 2883, 2914, 3064, 3256, 3546, 4147
TatI WGTACW 4 cut(s) 1072, 3694, 3842, 4701
TauI GCSGC 4 cut(s) 829, 1450, 2211, 4540
TseFI GTSAC 5 cut(s) 355, 598, 2765, 3613, 4651
Tsp45I GTSAC 5 cut(s) 355, 598, 2765, 3613, 4651
TspGWI ACGGA 3 cut(s) 979, 1479, 3717
Van91I CCANNNNNTGG 3 cut(s) 1605, 2624, 3284
Vha464I CTTAAG 1 cut(s) 1991
VneI GTGCAC 1 cut(s) 2337
VpaK11BI GGWCC 3 cut(s) 778, 1596, 4049
XagI CCTNNNNNAGG 3 cut(s) 15, 336, 915
XapI RAATTY 6 cut(s) 536, 727, 967, 1134, 1661, 3857
XceI RCATGY 4 cut(s) 1163, 2011, 3226, 4091
XmaJI CCTAGG 1 cut(s) 4082
XmiI GTMKAC 3 cut(s) 350, 1875, 4816
XmnI GAANNNNTTC 1 cut(s) 4455
ZrmI AGTACT 1 cut(s) 3696
Zsp2I ATGCAT 3 cut(s) 1614, 1767, 3998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.