Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0012828.1

Orthogroup ID

OG0006901

Physical Location & Seq

Genomic Coordinates Forward (+)

1 .. 2038

Gene Structure 2038 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0012828.1_g000001.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1776 bp

gaaccaaacctgggcaaggacaaagtgaaatcaagctcaactccggtatctcgctgccttgtttgtttggataagaacagttgtcgagtagttcgttcgagaacaaagctgatgaataatttgatttcaagagggaagcctcatgaagctcattccatcttcaataatttagtagaagaaggacataggccaactcttataacatacacaactctagttgctgccttgacccgccagaggcgtttcaagtcaattcccttgctcctctctgaagtggaggagaatggtatgaagcctgattcaatacttttcaatgccatgatcaatgctttctccgaatctggaaatgtaaatgatgccatgaaaatctttcgcaaaatggaggagagtggatgtaagcccacaaccagcacttttaacacccttatcaaaggatatggaattgctggtaaacctgaagaatctttaaaattgctagagcttatgttgcaggatgggaatgcgaaaccgaatgacaggacatataatattctagttagagcctggtgtaacaagaagagattagatcaagcctggaatgtggtgtataagatggttgcttctgggatacaacctgatgcagtcactttcaacacattggcaagggcttatgctgagaatggagagaccagtaaagctgaaaggttgatgttggagatgcaacacaacaaagttaatcccaacgagcgtacgtgtggcattgttgtaaatggatattgtaaagaaggtaacatgacagatgctctgaggtttgtatatagaatgaaggatcttggggtgaagccaaatctcgtcattttcaactctctcatcaaggggtttctgaacattacagacactgatggagttgatgaggtactgacattgatggaagaatttgggataaaaccagatgtgataacatttagcaccataatgaatgcatggagctcagcagggctaatggaaaaatgccaggagatctttgatgacatgataaaagcggacatagaaccagacatccattcatttggcattcttgcaaaaggctatgtgcgtgccggtgatccagcgaaggccgagtcacttctgagtttcatgggcaaatctggtgtgcatccaaatgttgtaatcttcacaaccatcatgagtgggtggtgcacagcaggaaaaatggagcatgcttggggtatctttgagaaaatgtgtgaaatcggaatcaccccaaatgtgaaaacttttgagactctaatatggggatatggggaagctagacagccatggaaagcagaagatttgcttcatataatggaagagaagggtattgttcctgaaatgagaacaatcgagcttgttgctgaggcttggcgtgctataggtttaatggatgaagcgatgagactcctcaatgagtcaaaagaggatccggaagtcatactaaacaaggcacctgaacagagtttagaggcaattagtcgaaaacaaagcttgaatacttcttatcctaatgctttgcagatacctggagtagttgtttctgataatggcagctctgctgctaacataagaggccagatgatttcaaaaagattcgagttttcatctgaaagtatgcagaatgttactagaaccatgtgtgttactcgtacatctgcactaagagtgcagccactggttatgtgcaggaggcagtttcaaagtcaagttgggatatgcaggcagtttgaaaatacatgtagagtggtgtatatatgttag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 Pkinase

Protein Analysis

591

Amino Acids

66.25

Weight (kDa)

8.11

Isoelectric Point (pI)

36.3

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006901)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04540 FvH4_1g04540 FvH4_1g04540 FvH4_1g04540 FvH4_1g04540
malus_domestica	MD15G1183500.v1.1
prunus_persica	Prupe.7G232600_v2.0.a1 Prupe.7G232600_v2.0.a1 Prupe.7G232600_v2.0.a1 Prupe.7G232600_v2.0.a1
pyrus_communis	pycom15g16530
rosa_chinensis	RchiOBHm_Chr2g0090251
rosa_laevigata	RLG00000016091
rosa_multiflora	Rmu_co8459707.1_g000001 Rmu_sc0012828.1_g000001
rosa_roxburghii	Rroxscaffold_2G00151350
rosa_rugosa	Rorug02G0005700 Rorug02G0005800 Rorug02G0005900 Rorug02G0006000 Rorug02G0006000
rosa_samantha	Rh2AG051800 Rh2BG050400 Rh2CG052600 Rh2DG051700
rosa_wichuraiana	Rw2G004510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	200
AccB1I	GGYRCC	1 cut(s)	1465
AccIII	TCCGGA	1 cut(s)	1444
AciI	CCGC	2 cut(s)	232, 1022
AclWI	GGATC	4 cut(s)	816, 1079, 1436, 1449
AcsI	RAATTY	1 cut(s)	914
AcuI	CTGAAG	2 cut(s)	291, 477
AfaI	GTAC	3 cut(s)	730, 897, 1666
AfiI	CCNNNNNNNGG	3 cut(s)	11, 16, 237
AflIII	ACRYGT	2 cut(s)	731, 1751
AjnI	CCWGG	5 cut(s)	9, 542, 572, 993, 1540
AloI	GAACNNNNNNTCC	2 cut(s)	1023, 1055
Alw21I	GWGCWC	2 cut(s)	971, 1181
Alw26I	GTCTC	3 cut(s)	659, 1256, 1411
Alw44I	GTGCAC	1 cut(s)	1177
AlwI	GGATC	4 cut(s)	816, 1079, 1436, 1449
AlwNI	CAGNNNCTG	2 cut(s)	878, 1690
Aor13HI	TCCGGA	1 cut(s)	1444
AoxI	GGCC	3 cut(s)	188, 1095, 1588
ApaLI	GTGCAC	1 cut(s)	1177
ApeKI	GCWGC	5 cut(s)	54, 221, 1566, 1574, 1684
ApoI	RAATTY	1 cut(s)	914
ArsI	GACNNNNNNTTYG	2 cut(s)	1085, 1117
AsuHPI	GGTGA	3 cut(s)	829, 1094, 1231
BaeGI	GKGCMC	1 cut(s)	1181
BaeI	ACNNNNGTAYC	2 cut(s)	30, 63
BamHI	GGATCC	1 cut(s)	1441
BanI	GGYRCC	1 cut(s)	1465
BanII	GRGCYC	1 cut(s)	971
Bbv12I	GWGCWC	2 cut(s)	971, 1181
BbvCI	CCTCAGC	1 cut(s)	1377
BbvI	GCAGC	5 cut(s)	41, 208, 1561, 1578, 1696
BccI	CCATC	6 cut(s)	164, 488, 586, 875, 901, 1169
BcgI	CGANNNNNNTGC	2 cut(s)	1355, 1389
BciT130I	CCWGG	5 cut(s)	11, 544, 574, 995, 1542
BciVI	GTATCC	1 cut(s)	600
BclI	TGATCA	1 cut(s)	321
BcoDI	GTCTC	3 cut(s)	659, 1256, 1411
BfaI	CTAG	5 cut(s)	215, 476, 533, 1290, 1644
BfmI	CTRYAG	1 cut(s)	1392
BfuI	GTATCC	1 cut(s)	600
BglII	AGATCT	1 cut(s)	999
BisI	GCNGC	5 cut(s)	55, 222, 1567, 1575, 1685
BlpI	GCTNAGC	1 cut(s)	970
BlsI	GCNGC	5 cut(s)	56, 223, 1568, 1576, 1686
Bme1390I	CCNGG	5 cut(s)	11, 544, 574, 995, 1542
BmiI	GGNNCC	2 cut(s)	1443, 1467
BmrFI	CCNGG	5 cut(s)	11, 544, 574, 995, 1542
BmsI	GCATC	5 cut(s)	346, 607, 687, 769, 1144
BpmI	CTGGAG	1 cut(s)	1563
Bpu10I	CCTNAGC	1 cut(s)	1377
Bpu1102I	GCTNAGC	1 cut(s)	970
BsaAI	YACGTR	1 cut(s)	732
BsaI	GGTCTC	1 cut(s)	659
BsaJI	CCNNGG	2 cut(s)	10, 1298
BsaWI	WCCGGW	2 cut(s)	43, 1444
BsaXI	ACNNNNNCTCC	2 cut(s)	1536, 1566
Bsc4I	CCNNNNNNNGG	3 cut(s)	11, 16, 237
Bse118I	RCCGGY	1 cut(s)	1079
Bse1I	ACTGG	2 cut(s)	669, 1695
BseAI	TCCGGA	1 cut(s)	1444
BseBI	CCWGG	5 cut(s)	11, 544, 574, 995, 1542
BseDI	CCNNGG	2 cut(s)	10, 1298
BseGI	GGATG	5 cut(s)	398, 499, 1038, 1135, 1411
BseLI	CCNNNNNNNGG	3 cut(s)	11, 16, 237
BseMII	CTCAG	5 cut(s)	645, 776, 984, 1100, 1368
BseNI	ACTGG	2 cut(s)	669, 1695
BseRI	GAGGAG	4 cut(s)	254, 293, 398, 1412
BseSI	GKGCMC	1 cut(s)	1181
BseXI	GCAGC	5 cut(s)	41, 208, 1561, 1578, 1696
BsgI	GTGCAG	3 cut(s)	1656, 1703, 1720
BshFI	GGCC	3 cut(s)	190, 1097, 1590
BshNI	GGYRCC	1 cut(s)	1465
BsiHKAI	GWGCWC	2 cut(s)	971, 1181
BsiSI	CCGG	3 cut(s)	44, 1080, 1445
BsiWI	CGTACG	1 cut(s)	728
BslI	CCNNNNNNNGG	3 cut(s)	11, 16, 237
BsmAI	GTCTC	3 cut(s)	659, 1256, 1411
BsmI	GAATGC	3 cut(s)	505, 964, 1053
BsnI	GGCC	3 cut(s)	190, 1097, 1590
Bso31I	GGTCTC	1 cut(s)	659
Bsp1286I	GDGCHC	2 cut(s)	971, 1181
Bsp13I	TCCGGA	1 cut(s)	1444
Bsp143I	GATC	6 cut(s)	321, 565, 808, 999, 1084, 1441
Bsp1720I	GCTNAGC	1 cut(s)	970
Bsp19I	CCATGG	1 cut(s)	1298
BspACI	CCGC	2 cut(s)	232, 1022
BspANI	GGCC	3 cut(s)	190, 1097, 1590
BspCNI	CTCAG	5 cut(s)	646, 777, 983, 1101, 1369
BspEI	TCCGGA	1 cut(s)	1444
BspHI	TCATGA	2 cut(s)	142, 1164
BspLI	GGNNCC	2 cut(s)	1443, 1467
BspPI	GGATC	4 cut(s)	816, 1079, 1436, 1449
BspT107I	GGYRCC	1 cut(s)	1465
BspTNI	GGTCTC	1 cut(s)	659
BsrFI	RCCGGY	1 cut(s)	1079
BsrI	ACTGG	2 cut(s)	669, 1695
BssAI	RCCGGY	1 cut(s)	1079
BssECI	CCNNGG	2 cut(s)	10, 1298
BssMI	GATC	6 cut(s)	321, 565, 808, 999, 1084, 1441
BssT1I	CCWWGG	1 cut(s)	1298
Bst2UI	CCWGG	5 cut(s)	11, 544, 574, 995, 1542
Bst4CI	ACNGT	1 cut(s)	80
Bst6I	CTCTTC	2 cut(s)	551, 1326
BstBAI	YACGTR	1 cut(s)	732
BstC8I	GCNNGC	4 cut(s)	1077, 1200, 1389, 1736
BstDEI	CTNAG	6 cut(s)	654, 785, 970, 1109, 1377, 1676
BstDSI	CCRYGG	1 cut(s)	1298
BstENI	CCTNNNNNAGG	1 cut(s)	14
BstF5I	GGATG	5 cut(s)	398, 499, 1038, 1135, 1411
BstKTI	GATC	6 cut(s)	324, 568, 811, 1002, 1087, 1444
BstMAI	GTCTC	3 cut(s)	659, 1256, 1411
BstMBI	GATC	6 cut(s)	321, 565, 808, 999, 1084, 1441
BstMWI	GCNNNNNNNGC	2 cut(s)	487, 1388
BstNI	CCWGG	5 cut(s)	11, 544, 574, 995, 1542
BstNSI	RCATGY	2 cut(s)	1202, 1755
BstSCI	CCNGG	5 cut(s)	9, 542, 572, 993, 1540
BstSFI	CTRYAG	1 cut(s)	1392
BstSLI	GKGCMC	1 cut(s)	1181
BstV1I	GCAGC	5 cut(s)	41, 208, 1561, 1578, 1696
BstX2I	RGATCY	3 cut(s)	808, 999, 1441
BstXI	CCANNNNNNTGG	1 cut(s)	1049
BstYI	RGATCY	3 cut(s)	808, 999, 1441
BsuI	GTATCC	1 cut(s)	600
BsuRI	GGCC	3 cut(s)	190, 1097, 1590
BtgI	CCRYGG	1 cut(s)	1298
BtgZI	GCGATG	1 cut(s)	1427
BtsCI	GGATG	5 cut(s)	398, 499, 1038, 1135, 1411
BtsIMutI	CAGTG	2 cut(s)	876, 1688
Cac8I	GCNNGC	4 cut(s)	1077, 1200, 1389, 1736
CaiI	CAGNNNCTG	2 cut(s)	878, 1690
CciI	TCATGA	2 cut(s)	142, 1164
Cfr10I	RCCGGY	1 cut(s)	1079
Csp6I	GTAC	3 cut(s)	729, 896, 1665
CviQI	GTAC	3 cut(s)	729, 896, 1665
DdeI	CTNAG	6 cut(s)	654, 785, 970, 1109, 1377, 1676
DpnI	GATC	6 cut(s)	323, 567, 810, 1001, 1086, 1443
DpnII	GATC	6 cut(s)	321, 565, 808, 999, 1084, 1441
DraI	TTTAAA	1 cut(s)	468
Eam1104I	CTCTTC	2 cut(s)	551, 1326
EarI	CTCTTC	2 cut(s)	551, 1326
Ecl136II	GAGCTC	1 cut(s)	969
Eco130I	CCWWGG	1 cut(s)	1298
Eco24I	GRGCYC	1 cut(s)	971
Eco31I	GGTCTC	1 cut(s)	659
Eco53kI	GAGCTC	1 cut(s)	969
Eco57I	CTGAAG	2 cut(s)	291, 477
EcoICRI	GAGCTC	1 cut(s)	969
EcoNI	CCTNNNNNAGG	1 cut(s)	14
EcoRII	CCWGG	5 cut(s)	9, 542, 572, 993, 1540
EcoT14I	CCWWGG	1 cut(s)	1298
EcoT22I	ATGCAT	1 cut(s)	964
EcoT38I	GRGCYC	1 cut(s)	971
ErhI	CCWWGG	1 cut(s)	1298
FalI	AAGNNNNNCTT	2 cut(s)	1302, 1334
FauI	CCCGC	1 cut(s)	239
FbaI	TGATCA	1 cut(s)	321
Fnu4HI	GCNGC	5 cut(s)	55, 222, 1567, 1575, 1685
FokI	GGATG	5 cut(s)	405, 506, 1025, 1122, 1418
FriOI	GRGCYC	1 cut(s)	971
Fsp4HI	GCNGC	5 cut(s)	55, 222, 1567, 1575, 1685
FspBI	CTAG	5 cut(s)	215, 476, 533, 1290, 1644
GluI	GCNGC	5 cut(s)	55, 222, 1567, 1575, 1685
GsuI	CTGGAG	1 cut(s)	1563
HaeIII	GGCC	3 cut(s)	190, 1097, 1590
HapII	CCGG	3 cut(s)	44, 1080, 1445
HindIII	AAGCTT	1 cut(s)	1504
HinfI	GANTC	9 cut(s)	299, 338, 461, 1100, 1236, 1264, 1419, 1430, 1608
HpaII	CCGG	3 cut(s)	44, 1080, 1445
HphI	GGTGA	3 cut(s)	829, 1094, 1231
Hpy166II	GTNNAC	2 cut(s)	452, 1179
Hpy188I	TCNGA	8 cut(s)	271, 337, 786, 864, 1110, 1235, 1558, 1624
Hpy188III	TCNNGA	7 cut(s)	99, 129, 143, 342, 1165, 1349, 1445
Hpy8I	GTNNAC	2 cut(s)	452, 1179
HpyAV	CCTTC	5 cut(s)	173, 758, 799, 1087, 1330
HpyCH4III	ACNGT	1 cut(s)	80
HpyCH4IV	ACGT	1 cut(s)	731
HpyF10VI	GCNNNNNNNGC	2 cut(s)	487, 1388
HpyF3I	CTNAG	6 cut(s)	654, 785, 970, 1109, 1377, 1676
HpySE526I	ACGT	1 cut(s)	731
Kpn2I	TCCGGA	1 cut(s)	1444
Ksp22I	TGATCA	1 cut(s)	321
Kzo9I	GATC	6 cut(s)	321, 565, 808, 999, 1084, 1441
LmnI	GCTCC	3 cut(s)	267, 966, 1195
Lsp1109I	GCAGC	5 cut(s)	41, 208, 1561, 1578, 1696
LweI	GCATC	5 cut(s)	346, 607, 687, 769, 1144
MaeI	CTAG	5 cut(s)	215, 476, 533, 1290, 1644
MaeII	ACGT	1 cut(s)	731
MaeIII	GTNAC	6 cut(s)	548, 622, 767, 1101, 1639, 1657
MalI	GATC	6 cut(s)	323, 567, 810, 1001, 1086, 1443
MboI	GATC	6 cut(s)	321, 565, 808, 999, 1084, 1441
MboII	GAAGA	8 cut(s)	151, 188, 470, 568, 923, 1144, 1322, 1343
MflI	RGATCY	3 cut(s)	808, 999, 1441
MhlI	GDGCHC	2 cut(s)	971, 1181
MluCI	AATT	7 cut(s)	118, 166, 252, 441, 470, 914, 1488
MlyI	GAGTC	4 cut(s)	1109, 1258, 1413, 1439
MmeI	TCCRAC	1 cut(s)	672
Mph1103I	ATGCAT	1 cut(s)	964
MroI	TCCGGA	1 cut(s)	1444
MseI	TTAA	4 cut(s)	417, 467, 714, 1400
MslI	CAYNNNNRTG	2 cut(s)	953, 1140
MspI	CCGG	3 cut(s)	44, 1080, 1445
MspR9I	CCNGG	5 cut(s)	11, 544, 574, 995, 1542
Mva1269I	GAATGC	3 cut(s)	505, 964, 1053
MvaI	CCWGG	5 cut(s)	11, 544, 574, 995, 1542
MwoI	GCNNNNNNNGC	2 cut(s)	487, 1388
NcoI	CCATGG	1 cut(s)	1298
NdeII	GATC	6 cut(s)	321, 565, 808, 999, 1084, 1441
NlaIV	GGNNCC	2 cut(s)	1443, 1467
NmeAIII	GCCGAG	1 cut(s)	1123
NmuCI	GTSAC	2 cut(s)	622, 1101
NsiI	ATGCAT	1 cut(s)	964
NspI	RCATGY	2 cut(s)	1202, 1755
PaeI	GCATGC	1 cut(s)	1202
PagI	TCATGA	2 cut(s)	142, 1164
PciI	ACATGT	1 cut(s)	1751
PcsI	WCGNNNNNNNCGW	1 cut(s)	91
PctI	GAATGC	3 cut(s)	505, 964, 1053
PfeI	GAWTC	5 cut(s)	299, 338, 461, 1236, 1608
Pfl23II	CGTACG	1 cut(s)	728
PkrI	GCNGC	5 cut(s)	56, 223, 1568, 1576, 1686
PleI	GAGTC	4 cut(s)	1108, 1258, 1413, 1438
PpsI	GAGTC	4 cut(s)	1108, 1258, 1413, 1438
Ppu21I	YACGTR	1 cut(s)	732
PscI	ACATGT	1 cut(s)	1751
PsiI	TTATAA	1 cut(s)	200
Psp124BI	GAGCTC	1 cut(s)	971
Psp6I	CCWGG	5 cut(s)	9, 542, 572, 993, 1540
PspGI	CCWGG	5 cut(s)	9, 542, 572, 993, 1540
PspLI	CGTACG	1 cut(s)	728
PspN4I	GGNNCC	2 cut(s)	1443, 1467
PstNI	CAGNNNCTG	2 cut(s)	878, 1690
PsuI	RGATCY	3 cut(s)	808, 999, 1441
RsaI	GTAC	3 cut(s)	730, 897, 1666
RsaNI	GTAC	3 cut(s)	729, 896, 1665
RseI	CAYNNNNRTG	2 cut(s)	953, 1140
SacI	GAGCTC	1 cut(s)	971
SaqAI	TTAA	4 cut(s)	417, 467, 714, 1400
SatI	GCNGC	5 cut(s)	55, 222, 1567, 1575, 1685
Sau3AI	GATC	6 cut(s)	321, 565, 808, 999, 1084, 1441
SchI	GAGTC	4 cut(s)	1109, 1258, 1413, 1439
ScrFI	CCNGG	5 cut(s)	11, 544, 574, 995, 1542
SduI	GDGCHC	2 cut(s)	971, 1181
SfaNI	GCATC	5 cut(s)	346, 607, 687, 769, 1144
SfcI	CTRYAG	1 cut(s)	1392
SmiMI	CAYNNNNRTG	2 cut(s)	953, 1140
SphI	GCATGC	1 cut(s)	1202
Sse9I	AATT	7 cut(s)	118, 166, 252, 441, 470, 914, 1488
SsiI	CCGC	2 cut(s)	232, 1022
SspI	AATATT	1 cut(s)	529
SspMI	CTAG	5 cut(s)	215, 476, 533, 1290, 1644
SstI	GAGCTC	1 cut(s)	971
StyD4I	CCNGG	5 cut(s)	9, 542, 572, 993, 1540
StyI	CCWWGG	1 cut(s)	1298
TaaI	ACNGT	1 cut(s)	80
TaiI	ACGT	1 cut(s)	734
TaqI	TCGA	5 cut(s)	85, 98, 1365, 1495, 1611
TasI	AATT	7 cut(s)	118, 166, 252, 441, 470, 914, 1488
TfiI	GAWTC	5 cut(s)	299, 338, 461, 1236, 1608
Tru1I	TTAA	4 cut(s)	417, 467, 714, 1400
Tru9I	TTAA	4 cut(s)	417, 467, 714, 1400
TscAI	CASTG	2 cut(s)	883, 1695
TseFI	GTSAC	2 cut(s)	622, 1101
TseI	GCWGC	5 cut(s)	54, 221, 1566, 1574, 1684
Tsp45I	GTSAC	2 cut(s)	622, 1101
TspRI	CASTG	2 cut(s)	883, 1695
VneI	GTGCAC	1 cut(s)	1177
XagI	CCTNNNNNAGG	1 cut(s)	14
XapI	RAATTY	1 cut(s)	914
XceI	RCATGY	2 cut(s)	1202, 1755
XspI	CTAG	5 cut(s)	215, 476, 533, 1290, 1644
Zsp2I	ATGCAT	1 cut(s)	964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.