RLG00000018748

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006596
Physical Location & Seq
Forward (+)
35265829 .. 35270665
4837 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018748

Sequence Viewer

Length: 1287 bp
ATGTGCAGCTCTCTCATTGATATTGCTCAAAAAGAAGAAGACGCAGCCCCCGAGTTTCGATTCAATCATTGGAAGAAGAACGGGATTTTGCAACTCCCCAGAGAAGCTACACCTAAACTTCCGTGTAGTAAATCTCAAGTAGATTCTTCAGTCGATCAACGTTTACATATTGACGCTTTCAGCCGACGTCTGAATACTTTTGCTTCTTCCGATCAGATTGACAGTACTAAACCTAGCATTAGTGTTGTTCAACTCGAGAAAGTTGATGTCGTTAGTTGCACTCCACCTGAAAATCCTACACTATCAGAAGTTTCTGCTGAAGTTAAGGTTGCTTACTCTGATACTTGTCAAGCTCCTGGTTCTCTTGTGCAGATTGTTATGGTGAAAAATGAGATCTCCGGGCACTGTACTTATGATCATCTGGACCACATTGCCTTGAAAGAGCAGCAACGGATGCTACAATCAAGTACTACTGGTCAATGTTCTGTTGAATTAGCTAAATCAAACTACTACAGGAAGAGTTCTGAAAGTGACGGCATATGCTCATCTGATCCATGTGGGAGACAAGATGATGGGCCTACAGGCACAAGTAGAGCGGATTCCTCGGTCAAAATAAAGGATGAACCTTTGGACAACATGACTTCCCCAGACCTAGAGCTCCCAAAAAAATTAGTGAAGATCGATGAGGTACACAATATGCTGCTGCAAGATTGGATGAATCTATTGGTGTCATGGGAGGACTCTGAAGTGAGTATGTCTAAGAACTATGCATGCTTGGTTAAAATTGTGCCTTCTGGCCTTAATTGTAGCCCTATTTCCATGGAATCTGCTAAACCTGGATTCATTAAAACGGCGTTGGAGGAGGATGCTCTGGGACTCTTGCAAGTACTCCATGTTTTTCATTTAATGGGGGGCGGGGGGGGTGTATTGGTTGATGAAATCAAGCTTTATGGTGAGGCGGAGAGTGATGAAGCTTTAGATGAATCTTTATGGTCTAATGTTAGCTTCCTTATCGTTGGTCAGATTTTCTCGCAGCACCAGTCATTTTTTAAGTTTGCTCCAATACGATGCACAAAGGACTCAAGGGCTAGTTATTGCTTGGTTTCTCTAATTTCACTTGCTGAGAAGGTTGGAGAAGACTTGAGTGAAGGAGAGGCCCAGGTTTTATTGGAATATATGGTTGGGAAGCCAACACTGGTCTGGGAACCATGCAAAAGTACCGTGACTGCAGAGTTGTGCATGACCGGAAGAATGAAGGTGAGAGCTCAGAGTAGAGATCAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

429

Amino Acids

47.2

Weight (kDa)

5.01

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 190
AccBSI CCGCTC 1 cut(s) 596
AciI CCGC 3 cut(s) 596, 915, 959
AclI AACGTT 1 cut(s) 160
AclWI GGATC 1 cut(s) 545
AcuI CTGAAG 3 cut(s) 132, 339, 765
AcyI GRCGYC 1 cut(s) 187
AfaI GTAC 6 cut(s) 226, 409, 469, 690, 888, 1219
AgsI TTSAA 4 cut(s) 64, 251, 439, 491
AjnI CCWGG 3 cut(s) 355, 835, 1158
AjuI GAANNNNNNNTTGG 2 cut(s) 1164, 1196
AluBI AGCT 9 cut(s) 9, 107, 353, 497, 658, 946, 974, 1005, 1265
AluI AGCT 9 cut(s) 9, 107, 353, 497, 658, 946, 974, 1005, 1265
Alw21I GWGCWC 2 cut(s) 660, 1267
Alw26I GTCTC 1 cut(s) 556
AlwI GGATC 1 cut(s) 545
Ama87I CYCGRG 2 cut(s) 50, 254
AoxI GGCC 3 cut(s) 575, 796, 1155
ApeKI GCWGC 6 cut(s) 6, 44, 445, 700, 703, 1033
AspS9I GGNCC 3 cut(s) 424, 575, 1156
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 3 cut(s) 394, 965, 1270
AvaI CYCGRG 2 cut(s) 50, 254
AvaII GGWCC 1 cut(s) 424
BaeGI GKGCMC 1 cut(s) 405
BanII GRGCYC 2 cut(s) 660, 1267
BbsI GAAGAC 2 cut(s) 45, 1143
Bbv12I GWGCWC 2 cut(s) 660, 1267
BbvI GCAGC 6 cut(s) 18, 56, 457, 687, 690, 1045
BccI CCATC 1 cut(s) 566
BceAI ACGGC 2 cut(s) 550, 867
BciT130I CCWGG 3 cut(s) 357, 837, 1160
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 556
BfaI CTAG 3 cut(s) 234, 653, 1089
BfmI CTRYAG 3 cut(s) 511, 579, 1227
BglII AGATCT 1 cut(s) 393
BisI GCNGC 6 cut(s) 7, 45, 446, 701, 704, 1034
BlsI GCNGC 6 cut(s) 8, 46, 447, 702, 705, 1035
BmcAI AGTACT 3 cut(s) 226, 469, 888
Bme1390I CCNGG 4 cut(s) 357, 400, 837, 1160
Bme18I GGWCC 1 cut(s) 424
BmeT110I CYCGRG 2 cut(s) 50, 254
BmgT120I GGNCC 3 cut(s) 424, 575, 1156
BmiI GGNNCC 1 cut(s) 1206
BmrFI CCNGG 4 cut(s) 357, 400, 837, 1160
BmsI GCATC 3 cut(s) 444, 856, 1058
BpiI GAAGAC 2 cut(s) 45, 1143
BpuEI CTTGAG 3 cut(s) 120, 1066, 1162
BpuMI CCSGG 1 cut(s) 400
Bsa29I ATCGAT 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 187
BsaJI CCNNGG 3 cut(s) 603, 819, 1158
BsaWI WCCGGW 1 cut(s) 1244
Bse1I ACTGG 3 cut(s) 478, 1039, 1200
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 3 cut(s) 357, 837, 1160
BseCI ATCGAT 1 cut(s) 681
BseDI CCNNGG 3 cut(s) 603, 819, 1158
BseGI GGATG 4 cut(s) 459, 625, 720, 871
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 1113, 1280
BseNI ACTGG 3 cut(s) 478, 1039, 1200
BseRI GAGGAG 1 cut(s) 875
BseSI GKGCMC 1 cut(s) 405
BseXI GCAGC 6 cut(s) 18, 56, 457, 687, 690, 1045
BsgI GTGCAG 2 cut(s) 25, 389
BshFI GGCC 3 cut(s) 577, 798, 1157
BshVI ATCGAT 1 cut(s) 681
BsiHKAI GWGCWC 2 cut(s) 660, 1267
BsiHKCI CYCGRG 2 cut(s) 50, 254
BsiSI CCGG 2 cut(s) 399, 1245
BslFI GGGAC 1 cut(s) 888
BsmAI GTCTC 1 cut(s) 556
BsmFI GGGAC 1 cut(s) 888
BsnI GGCC 3 cut(s) 577, 798, 1157
BsoBI CYCGRG 2 cut(s) 50, 254
Bsp1286I GDGCHC 3 cut(s) 405, 660, 1267
Bsp143I GATC 7 cut(s) 154, 211, 393, 415, 550, 678, 1276
Bsp19I CCATGG 1 cut(s) 819
BspACI CCGC 3 cut(s) 596, 915, 959
BspANI GGCC 3 cut(s) 577, 798, 1157
BspCNI CTCAG 2 cut(s) 1114, 1279
BspDI ATCGAT 1 cut(s) 681
BspLI GGNNCC 1 cut(s) 1206
BspMAI CTGCAG 1 cut(s) 1231
BspPI GGATC 1 cut(s) 545
BsrBI CCGCTC 1 cut(s) 596
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 3 cut(s) 478, 1039, 1200
BssECI CCNNGG 3 cut(s) 603, 819, 1158
BssMI GATC 7 cut(s) 154, 211, 393, 415, 550, 678, 1276
BssNI GRCGYC 1 cut(s) 187
BssT1I CCWWGG 1 cut(s) 819
Bst2UI CCWGG 3 cut(s) 357, 837, 1160
Bst4CI ACNGT 3 cut(s) 224, 407, 1222
Bst6I CTCTTC 1 cut(s) 512
BstACI GRCGYC 1 cut(s) 187
BstAPI GCANNNNNTGC 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 772
BstDEI CTNAG 3 cut(s) 759, 1122, 1266
BstDSI CCRYGG 1 cut(s) 819
BstF5I GGATG 4 cut(s) 459, 625, 720, 871
BstKTI GATC 7 cut(s) 157, 214, 396, 418, 553, 681, 1279
BstMAI GTCTC 1 cut(s) 556
BstMBI GATC 7 cut(s) 154, 211, 393, 415, 550, 678, 1276
BstMWI GCNNNNNNNGC 1 cut(s) 454
BstNI CCWGG 3 cut(s) 357, 837, 1160
BstNSI RCATGY 1 cut(s) 774
BstSCI CCNGG 4 cut(s) 355, 398, 835, 1158
BstSFI CTRYAG 3 cut(s) 511, 579, 1227
BstSLI GKGCMC 1 cut(s) 405
BstV1I GCAGC 6 cut(s) 18, 56, 457, 687, 690, 1045
BstV2I GAAGAC 2 cut(s) 45, 1143
BstX2I RGATCY 1 cut(s) 393
BstYI RGATCY 1 cut(s) 393
Bsu15I ATCGAT 1 cut(s) 681
BsuRI GGCC 3 cut(s) 577, 798, 1157
BsuTUI ATCGAT 1 cut(s) 681
BtgI CCRYGG 1 cut(s) 819
BtsCI GGATG 4 cut(s) 459, 625, 720, 871
BtsIMutI CAGTG 2 cut(s) 403, 1193
Cac8I GCNNGC 1 cut(s) 772
Cfr13I GGNCC 3 cut(s) 424, 575, 1156
ClaI ATCGAT 1 cut(s) 681
CseI GACGC 2 cut(s) 50, 182
Csp6I GTAC 6 cut(s) 225, 408, 468, 689, 887, 1218
CviAII CATG 8 cut(s) 555, 637, 732, 771, 820, 893, 1209, 1240
CviQI GTAC 6 cut(s) 225, 408, 468, 689, 887, 1218
DdeI CTNAG 3 cut(s) 759, 1122, 1266
DpnI GATC 7 cut(s) 156, 213, 395, 417, 552, 680, 1278
DpnII GATC 7 cut(s) 154, 211, 393, 415, 550, 678, 1276
Eam1104I CTCTTC 1 cut(s) 512
EarI CTCTTC 1 cut(s) 512
EciI GGCGGA 1 cut(s) 974
Ecl136II GAGCTC 2 cut(s) 658, 1265
Eco130I CCWWGG 1 cut(s) 819
Eco24I GRGCYC 2 cut(s) 660, 1267
Eco47I GGWCC 1 cut(s) 424
Eco53kI GAGCTC 2 cut(s) 658, 1265
Eco57I CTGAAG 3 cut(s) 132, 339, 765
Eco88I CYCGRG 2 cut(s) 50, 254
EcoICRI GAGCTC 2 cut(s) 658, 1265
EcoRII CCWGG 3 cut(s) 355, 835, 1158
EcoT14I CCWWGG 1 cut(s) 819
EcoT22I ATGCAT 1 cut(s) 772
EcoT38I GRGCYC 2 cut(s) 660, 1267
ErhI CCWWGG 1 cut(s) 819
FaeI CATG 8 cut(s) 558, 640, 735, 774, 823, 896, 1212, 1243
FaqI GGGAC 1 cut(s) 888
FatI CATG 8 cut(s) 554, 636, 731, 770, 819, 892, 1208, 1239
FauI CCCGC 1 cut(s) 908
FauNDI CATATG 1 cut(s) 539
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 6 cut(s) 7, 45, 446, 701, 704, 1034
FokI GGATG 4 cut(s) 466, 632, 727, 878
FriOI GRGCYC 2 cut(s) 660, 1267
Fsp4HI GCNGC 6 cut(s) 7, 45, 446, 701, 704, 1034
FspBI CTAG 3 cut(s) 234, 653, 1089
GluI GCNGC 6 cut(s) 7, 45, 446, 701, 704, 1034
HaeIII GGCC 3 cut(s) 577, 798, 1157
HapII CCGG 2 cut(s) 399, 1245
HgaI GACGC 2 cut(s) 50, 182
Hin1I GRCGYC 1 cut(s) 187
Hin1II CATG 8 cut(s) 558, 640, 735, 774, 823, 896, 1212, 1243
HindIII AAGCTT 2 cut(s) 944, 972
HpaII CCGG 2 cut(s) 399, 1245
HphI GGTGA 3 cut(s) 394, 965, 1270
Hpy166II GTNNAC 2 cut(s) 164, 691
Hpy188III TCNNGA 2 cut(s) 256, 422
Hpy8I GTNNAC 2 cut(s) 164, 691
Hpy99I CGWCG 1 cut(s) 189
HpyAV CCTTC 4 cut(s) 801, 1120, 1142, 1249
HpyCH4III ACNGT 3 cut(s) 224, 407, 1222
HpyCH4IV ACGT 2 cut(s) 160, 187
HpyF10VI GCNNNNNNNGC 1 cut(s) 454
HpyF3I CTNAG 3 cut(s) 759, 1122, 1266
HpySE526I ACGT 2 cut(s) 160, 187
Hsp92I GRCGYC 1 cut(s) 187
Hsp92II CATG 8 cut(s) 558, 640, 735, 774, 823, 896, 1212, 1243
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 7 cut(s) 154, 211, 393, 415, 550, 678, 1276
LmnI GCTCC 3 cut(s) 358, 663, 1063
Lsp1109I GCAGC 6 cut(s) 18, 56, 457, 687, 690, 1045
LweI GCATC 3 cut(s) 444, 856, 1058
MaeI CTAG 3 cut(s) 234, 653, 1089
MaeII ACGT 2 cut(s) 160, 187
MaeIII GTNAC 2 cut(s) 530, 1222
MalI GATC 7 cut(s) 156, 213, 395, 417, 552, 680, 1278
MbiI CCGCTC 1 cut(s) 596
MboI GATC 7 cut(s) 154, 211, 393, 415, 550, 678, 1276
MflI RGATCY 1 cut(s) 393
MhlI GDGCHC 3 cut(s) 405, 660, 1267
MluCI AATT 5 cut(s) 491, 668, 783, 802, 1110
MlyI GAGTC 3 cut(s) 734, 870, 1073
MmeI TCCRAC 2 cut(s) 837, 1111
MnlI CCTC 7 cut(s) 613, 679, 730, 853, 856, 949, 1147
Mph1103I ATGCAT 1 cut(s) 772
MseI TTAA 6 cut(s) 324, 780, 801, 846, 905, 1050
MspI CCGG 2 cut(s) 399, 1245
MspR9I CCNGG 4 cut(s) 357, 400, 837, 1160
MvaI CCWGG 3 cut(s) 357, 837, 1160
MwoI GCNNNNNNNGC 1 cut(s) 454
NciI CCSGG 1 cut(s) 400
NcoI CCATGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 539
NdeII GATC 7 cut(s) 154, 211, 393, 415, 550, 678, 1276
NlaIII CATG 8 cut(s) 558, 640, 735, 774, 823, 896, 1212, 1243
NlaIV GGNNCC 1 cut(s) 1206
NmuCI GTSAC 2 cut(s) 530, 1222
NsiI ATGCAT 1 cut(s) 772
NspI RCATGY 1 cut(s) 774
PaeI GCATGC 1 cut(s) 774
PaeR7I CTCGAG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PfeI GAWTC 7 cut(s) 60, 143, 599, 718, 824, 840, 983
PkrI GCNGC 6 cut(s) 8, 46, 447, 702, 705, 1035
PleI GAGTC 3 cut(s) 734, 870, 1073
PpsI GAGTC 3 cut(s) 734, 870, 1073
Psp124BI GAGCTC 2 cut(s) 660, 1267
Psp1406I AACGTT 1 cut(s) 160
Psp6I CCWGG 3 cut(s) 355, 835, 1158
PspGI CCWGG 3 cut(s) 355, 835, 1158
PspN4I GGNNCC 1 cut(s) 1206
PspPI GGNCC 3 cut(s) 424, 575, 1156
PstI CTGCAG 1 cut(s) 1231
PsuI RGATCY 1 cut(s) 393
RsaI GTAC 6 cut(s) 226, 409, 469, 690, 888, 1219
RsaNI GTAC 6 cut(s) 225, 408, 468, 689, 887, 1218
SacI GAGCTC 2 cut(s) 660, 1267
SaqAI TTAA 6 cut(s) 324, 780, 801, 846, 905, 1050
SatI GCNGC 6 cut(s) 7, 45, 446, 701, 704, 1034
Sau3AI GATC 7 cut(s) 154, 211, 393, 415, 550, 678, 1276
Sau96I GGNCC 3 cut(s) 424, 575, 1156
ScaI AGTACT 3 cut(s) 226, 469, 888
SchI GAGTC 3 cut(s) 734, 870, 1073
ScrFI CCNGG 4 cut(s) 357, 400, 837, 1160
SduI GDGCHC 3 cut(s) 405, 660, 1267
SfaNI GCATC 3 cut(s) 444, 856, 1058
SfcI CTRYAG 3 cut(s) 511, 579, 1227
Sfr274I CTCGAG 1 cut(s) 254
SinI GGWCC 1 cut(s) 424
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 4 cut(s) 135, 254, 1081, 1141
SmoI CTYRAG 4 cut(s) 135, 254, 1081, 1141
SphI GCATGC 1 cut(s) 774
Sse9I AATT 5 cut(s) 491, 668, 783, 802, 1110
SsiI CCGC 3 cut(s) 596, 915, 959
SspMI CTAG 3 cut(s) 234, 653, 1089
SstI GAGCTC 2 cut(s) 660, 1267
StyD4I CCNGG 4 cut(s) 355, 398, 835, 1158
StyI CCWWGG 1 cut(s) 819
TaaI ACNGT 3 cut(s) 224, 407, 1222
TaiI ACGT 2 cut(s) 163, 190
TaqI TCGA 4 cut(s) 58, 153, 255, 681
TaqII GACCGA 1 cut(s) 595
TasI AATT 5 cut(s) 491, 668, 783, 802, 1110
TatI WGTACW 4 cut(s) 224, 407, 467, 886
TfiI GAWTC 7 cut(s) 60, 143, 599, 718, 824, 840, 983
Tru1I TTAA 6 cut(s) 324, 780, 801, 846, 905, 1050
Tru9I TTAA 6 cut(s) 324, 780, 801, 846, 905, 1050
TscAI CASTG 2 cut(s) 410, 1200
TseFI GTSAC 2 cut(s) 530, 1222
TseI GCWGC 6 cut(s) 6, 44, 445, 700, 703, 1033
Tsp45I GTSAC 2 cut(s) 530, 1222
TspDTI ATGAA 8 cut(s) 636, 731, 832, 890, 951, 984, 996, 1268
TspGWI ACGGA 2 cut(s) 111, 466
TspRI CASTG 2 cut(s) 410, 1200
VpaK11BI GGWCC 1 cut(s) 424
XceI RCATGY 1 cut(s) 774
XcmI CCANNNNNNNNNTGG 1 cut(s) 1197
XhoI CTCGAG 1 cut(s) 254
XspI CTAG 3 cut(s) 234, 653, 1089
ZraI GACGTC 1 cut(s) 188
ZrmI AGTACT 3 cut(s) 226, 469, 888
Zsp2I ATGCAT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.