Rorug02G0245600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006596
Physical Location & Seq
Reverse (-)
25518114 .. 25518736
623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0245600.1

Sequence Viewer

Length: 348 bp
ATGCTCCATTTCCAACTCTATATAAGCTCTATTAACATTACATTTCCTCTTCATCAACAACCGGCCTGTATCTCTTTTCTTCGTCTACATTCTACACATACAGAGATCAACATGGACCGTTCAATACGTCTAGCTGCCTTCCTTCTCGTCCTGGTTCTCATGGCAACTGAGATGGGGCGGACAATTGTTGCCAGGGAAAATCTGAGGACATGTGATTCTCCGAGTGGCAAGTTCAAGGGACCTTGCATTATAAGTAGCAACTGCGCACTTACCTGCCAAAAAGAAGGCTTCGCCGGCGGCCATTGCCGCGATTTCCTACGAAAATGCTACTGCGCTAAACCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

115

Amino Acids

12.91

Weight (kDa)

8.98

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gamma-thionin PF00304 69 - 115 2.9e-17 Gamma-thionin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
Acc16I TGCGCA 1 cut(s) 265
Acc36I ACCTGC 1 cut(s) 281
AccI GTMKAC 1 cut(s) 85
AccII CGCG 1 cut(s) 309
AciI CCGC 3 cut(s) 178, 297, 307
AcoI YGGCCR 1 cut(s) 298
AflIII ACRYGT 1 cut(s) 209
AgsI TTSAA 2 cut(s) 123, 235
AjnI CCWGG 2 cut(s) 150, 191
AluBI AGCT 2 cut(s) 27, 134
AluI AGCT 2 cut(s) 27, 134
AoxI GGCC 2 cut(s) 63, 298
ApeKI GCWGC 1 cut(s) 134
AspLEI GCGC 2 cut(s) 266, 335
AspS9I GGNCC 2 cut(s) 115, 239
AvaII GGWCC 2 cut(s) 115, 239
BbvI GCAGC 1 cut(s) 121
BccI CCATC 1 cut(s) 166
BciT130I CCWGG 2 cut(s) 152, 193
BfaI CTAG 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 281
BisI GCNGC 3 cut(s) 135, 298, 307
BlsI GCNGC 3 cut(s) 136, 299, 308
Bme1390I CCNGG 2 cut(s) 152, 193
Bme18I GGWCC 2 cut(s) 115, 239
BmgT120I GGNCC 2 cut(s) 115, 239
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 2 cut(s) 152, 193
BsaJI CCNNGG 1 cut(s) 192
Bse118I RCCGGY 2 cut(s) 61, 293
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 2 cut(s) 152, 193
BseDI CCNNGG 1 cut(s) 192
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 2 cut(s) 159, 194
BseXI GCAGC 1 cut(s) 121
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 2 cut(s) 65, 300
BsiSI CCGG 2 cut(s) 62, 294
BslFI GGGAC 1 cut(s) 252
BsmFI GGGAC 1 cut(s) 252
BsnI GGCC 2 cut(s) 65, 300
Bsp143I GATC 1 cut(s) 105
BspACI CCGC 3 cut(s) 178, 297, 307
BspANI GGCC 2 cut(s) 65, 300
BspCNI CTCAG 2 cut(s) 160, 195
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 240
BspMI ACCTGC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 301
BsrFI RCCGGY 2 cut(s) 61, 293
BssAI RCCGGY 2 cut(s) 61, 293
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 1 cut(s) 105
Bst2UI CCWGG 2 cut(s) 152, 193
Bst4CI ACNGT 1 cut(s) 119
Bst6I CTCTTC 1 cut(s) 54
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 2 cut(s) 168, 203
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 2 cut(s) 266, 335
BstKTI GATC 1 cut(s) 108
BstMBI GATC 1 cut(s) 105
BstMWI GCNNNNNNNGC 3 cut(s) 294, 303, 306
BstNI CCWGG 2 cut(s) 152, 193
BstNSI RCATGY 1 cut(s) 213
BstSCI CCNGG 2 cut(s) 150, 191
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 121
BsuRI GGCC 2 cut(s) 65, 300
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 295
CfoI GCGC 2 cut(s) 266, 335
Cfr10I RCCGGY 2 cut(s) 61, 293
Cfr13I GGNCC 2 cut(s) 115, 239
CviAII CATG 3 cut(s) 112, 160, 210
CviJI RGCY 5 cut(s) 27, 65, 134, 288, 300
CviKI_1 RGCY 5 cut(s) 27, 65, 134, 288, 300
DdeI CTNAG 2 cut(s) 168, 203
DpnI GATC 1 cut(s) 107
DpnII GATC 1 cut(s) 105
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
EciI GGCGGA 1 cut(s) 193
Eco47I GGWCC 2 cut(s) 115, 239
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 2 cut(s) 150, 191
FaeI CATG 3 cut(s) 115, 163, 213
FaiI YATR 7 cut(s) 21, 23, 99, 113, 161, 211, 251
FaqI GGGAC 1 cut(s) 252
FatI CATG 3 cut(s) 111, 159, 209
FblI GTMKAC 1 cut(s) 85
Fnu4HI GCNGC 3 cut(s) 135, 298, 307
Fsp4HI GCNGC 3 cut(s) 135, 298, 307
FspBI CTAG 1 cut(s) 131
FspI TGCGCA 1 cut(s) 265
GlaI GCGC 2 cut(s) 265, 334
GluI GCNGC 3 cut(s) 135, 298, 307
HaeIII GGCC 2 cut(s) 65, 300
HapII CCGG 2 cut(s) 62, 294
HhaI GCGC 2 cut(s) 266, 335
Hin1II CATG 3 cut(s) 115, 163, 213
Hin6I GCGC 2 cut(s) 264, 333
HinP1I GCGC 2 cut(s) 264, 333
HinfI GANTC 1 cut(s) 215
HpaII CCGG 2 cut(s) 62, 294
Hpy166II GTNNAC 1 cut(s) 86
Hpy188I TCNGA 2 cut(s) 204, 222
Hpy8I GTNNAC 1 cut(s) 86
HpyAV CCTTC 3 cut(s) 148, 152, 278
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 1 cut(s) 246
HpyF10VI GCNNNNNNNGC 3 cut(s) 294, 303, 306
HpyF3I CTNAG 2 cut(s) 168, 203
HpySE526I ACGT 1 cut(s) 127
Hsp92II CATG 3 cut(s) 115, 163, 213
HspAI GCGC 2 cut(s) 264, 333
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Kzo9I GATC 1 cut(s) 105
LmnI GCTCC 1 cut(s) 9
LpnPI CCDG 8 cut(s) 75, 79, 137, 164, 178, 205, 286, 307
Lsp1109I GCAGC 1 cut(s) 121
MaeI CTAG 1 cut(s) 131
MaeII ACGT 1 cut(s) 127
MalI GATC 1 cut(s) 107
MboI GATC 1 cut(s) 105
MboII GAAGA 2 cut(s) 41, 71
MfeI CAATTG 1 cut(s) 183
MluCI AATT 1 cut(s) 183
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 2 cut(s) 57, 198
MreI CGCCGGCG 1 cut(s) 293
MroNI GCCGGC 1 cut(s) 293
MseI TTAA 1 cut(s) 33
MspI CCGG 2 cut(s) 62, 294
MspR9I CCNGG 2 cut(s) 152, 193
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 2 cut(s) 152, 193
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 3 cut(s) 294, 303, 306
NaeI GCCGGC 1 cut(s) 295
NdeII GATC 1 cut(s) 105
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 3 cut(s) 115, 163, 213
NlaIV GGNNCC 1 cut(s) 240
NsbI TGCGCA 1 cut(s) 265
NspI RCATGY 1 cut(s) 213
PciI ACATGT 1 cut(s) 209
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 215
PkrI GCNGC 3 cut(s) 136, 299, 308
PpuMI RGGWCCY 1 cut(s) 239
PscI ACATGT 1 cut(s) 209
PsiI TTATAA 1 cut(s) 251
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 150, 191
PspGI CCWGG 2 cut(s) 150, 191
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 2 cut(s) 115, 239
PspPPI RGGWCCY 1 cut(s) 239
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 3 cut(s) 135, 298, 307
Sau3AI GATC 1 cut(s) 105
Sau96I GGNCC 2 cut(s) 115, 239
ScrFI CCNGG 2 cut(s) 152, 193
SetI ASST 6 cut(s) 29, 130, 136, 244, 275, 343
SgrAI CRCCGGYG 1 cut(s) 293
SinI GGWCC 2 cut(s) 115, 239
Sse9I AATT 1 cut(s) 183
SsiI CCGC 3 cut(s) 178, 297, 307
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 150, 191
TaaI ACNGT 1 cut(s) 119
TaiI ACGT 1 cut(s) 130
TasI AATT 1 cut(s) 183
TauI GCSGC 2 cut(s) 300, 309
TfiI GAWTC 1 cut(s) 215
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TseI GCWGC 1 cut(s) 134
TspDTI ATGAA 1 cut(s) 41
VpaK11BI GGWCC 2 cut(s) 115, 239
XceI RCATGY 1 cut(s) 213
XmiI GTMKAC 1 cut(s) 85
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.