RLG00000019649

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010951
Physical Location & Seq
Reverse (-)
54839191 .. 54840191
1001 bp
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UTR
Exon/CDS
Intron
RLM00000019649

Sequence Viewer

Length: 588 bp
ATGTCACCGGAAAATGTGCAACAGTCGCCGGCCGAGTTAAACTACGACGAGACGAAGCTGACCCTGGGATTGCCTGGCTCTGGCTCTAAACGTGGCTTCTCCGAGACCACCGTCGTCGGTCTCAATCTTGGGAGCTCATGCTCGTCGTCAACAACTGTTGATGATGATGTGAAAACTCAGAGACCTCCAGCCAAGGCACAAGTGGTGGGGTGGCCGCCTGTGAGAGGGTCTAGGAGGAACTTGATGACGAGCTGCAAGTATGTGAAGGTTGCAGTAGATGGAGCTCCGTACTTGCGCAAAATGGATCTTGAGACGTGCAAGAGCTATCAGCAGCTTTTGGGGGCTCTAGAGGATTTGTTCTCCTTCTTGACCATCCGTAATGATACGAATGAGAGAAAGCTTATGGACCTTGCTAATGGAGTGGAATATGTACCTACTTATGAGGACAGAGATGGGGATTGGATGCTTGTTGGAGATGTACCATGGAATATGTTTGTGGAGTCATGCAAGCGCCTAAGGTTGATGAAAAGCTCAGAGGCGATTGGATTAGCTCCGAGAACACCTTCAAAATGCACAAGCAGCAGCTGA

Protein Analysis

196

Amino Acids

21.51

Weight (kDa)

6.36

Isoelectric Point (pI)

56.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 17 - 186 2e-58 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010951)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80390
fragaria_vesca FvH4_6g30850 FvH4_6g30850 FvH4_6g30850 FvH4_6g30850
malus_domestica MD09G1216100.v1.1 MD17G1198300.v1.1
prunus_persica Prupe.3G074900_v2.0.a1 Prupe.3G074900_v2.0.a1
pyrus_communis pycom09g13300 pycom17g20310
rosa_chinensis RchiOBHm_Chr2g0137301
rosa_laevigata RLG00000019649
rosa_multiflora Rmu_sc0019961.1_g000004
rosa_roxburghii Rroxscaffold_2G00107220
rosa_rugosa Rorug02G0340900
rosa_samantha Rh2AG389200 Rh2BG397000 Rh2DG412000
rosa_wichuraiana Rw2G031910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 296
AciI CCGC 1 cut(s) 215
AclWI GGATC 1 cut(s) 312
AcoI YGGCCR 2 cut(s) 30, 212
AfaI GTAC 3 cut(s) 290, 432, 480
AfiI CCNNNNNNNGG 2 cut(s) 80, 224
AgsI TTSAA 1 cut(s) 567
AjiI CACGTC 1 cut(s) 315
AjnI CCWGG 2 cut(s) 63, 73
Alw21I GWGCWC 2 cut(s) 137, 286
Alw26I GTCTC 5 cut(s) 44, 98, 125, 175, 305
AlwI GGATC 1 cut(s) 312
AlwNI CAGNNNCTG 1 cut(s) 585
AoxI GGCC 2 cut(s) 30, 212
ApeKI GCWGC 4 cut(s) 252, 331, 579, 582
Asp700I GAANNNNTTC 1 cut(s) 562
AspLEI GCGC 2 cut(s) 297, 513
AspS9I GGNCC 1 cut(s) 406
AvaII GGWCC 1 cut(s) 406
AxyI CCTNAGG 1 cut(s) 515
BanII GRGCYC 3 cut(s) 137, 286, 346
Bbv12I GWGCWC 2 cut(s) 137, 286
BbvI GCAGC 2 cut(s) 239, 343
BccI CCATC 3 cut(s) 272, 380, 446
BciT130I CCWGG 2 cut(s) 65, 75
BcoDI GTCTC 5 cut(s) 44, 98, 125, 175, 305
BfaI CTAG 2 cut(s) 231, 347
BfoI RGCGCY 1 cut(s) 514
BisI GCNGC 5 cut(s) 215, 253, 332, 580, 583
BlsI GCNGC 5 cut(s) 216, 254, 333, 581, 584
Bme1390I CCNGG 2 cut(s) 65, 75
Bme18I GGWCC 1 cut(s) 406
BmgBI CACGTC 1 cut(s) 315
BmgT120I GGNCC 1 cut(s) 406
BmrFI CCNGG 2 cut(s) 65, 75
BmsI GCATC 1 cut(s) 453
BoxI GACNNNNGTC 1 cut(s) 110
BpmI CTGGAG 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 329
BsaI GGTCTC 3 cut(s) 98, 125, 175
BsaJI CCNNGG 4 cut(s) 63, 64, 192, 482
BsaWI WCCGGW 1 cut(s) 7
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 224
Bse118I RCCGGY 1 cut(s) 28
Bse21I CCTNAGG 1 cut(s) 515
BseBI CCWGG 2 cut(s) 65, 75
BseDI CCNNGG 4 cut(s) 63, 64, 192, 482
BseGI GGATG 2 cut(s) 372, 468
BseLI CCNNNNNNNGG 2 cut(s) 80, 224
BseMII CTCAG 2 cut(s) 191, 546
BseX3I CGGCCG 1 cut(s) 30
BseXI GCAGC 2 cut(s) 239, 343
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 2 cut(s) 32, 214
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 2 cut(s) 137, 286
BsiSI CCGG 2 cut(s) 8, 29
BslI CCNNNNNNNGG 2 cut(s) 80, 224
BsmAI GTCTC 5 cut(s) 44, 98, 125, 175, 305
BsmBI CGTCTC 2 cut(s) 44, 305
BsnI GGCC 2 cut(s) 32, 214
Bso31I GGTCTC 3 cut(s) 98, 125, 175
Bsp1286I GDGCHC 3 cut(s) 137, 286, 346
Bsp143I GATC 1 cut(s) 304
Bsp19I CCATGG 1 cut(s) 482
BspACI CCGC 1 cut(s) 215
BspANI GGCC 2 cut(s) 32, 214
BspCNI CTCAG 2 cut(s) 190, 545
BspPI GGATC 1 cut(s) 312
BspTNI GGTCTC 3 cut(s) 98, 125, 175
BsrFI RCCGGY 1 cut(s) 28
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 4 cut(s) 63, 64, 192, 482
BssMI GATC 1 cut(s) 304
BssT1I CCWWGG 2 cut(s) 192, 482
Bst2UI CCWGG 2 cut(s) 65, 75
Bst4CI ACNGT 3 cut(s) 24, 112, 157
BstC8I GCNNGC 2 cut(s) 30, 509
BstDEI CTNAG 3 cut(s) 177, 515, 532
BstDSI CCRYGG 1 cut(s) 482
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 2 cut(s) 372, 468
BstH2I RGCGCY 1 cut(s) 514
BstHHI GCGC 2 cut(s) 297, 513
BstKTI GATC 1 cut(s) 307
BstMAI GTCTC 5 cut(s) 44, 98, 125, 175, 305
BstMBI GATC 1 cut(s) 304
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 2 cut(s) 25, 579
BstNI CCWGG 2 cut(s) 65, 75
BstPAI GACNNNNGTC 1 cut(s) 110
BstSCI CCNGG 2 cut(s) 63, 73
BstV1I GCAGC 2 cut(s) 239, 343
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
BstZI CGGCCG 1 cut(s) 30
Bsu36I CCTNAGG 1 cut(s) 515
BsuRI GGCC 2 cut(s) 32, 214
BtgI CCRYGG 1 cut(s) 482
BtrI CACGTC 1 cut(s) 315
BtsCI GGATG 2 cut(s) 372, 468
Cac8I GCNNGC 2 cut(s) 30, 509
CaiI CAGNNNCTG 1 cut(s) 585
CfoI GCGC 2 cut(s) 297, 513
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 1 cut(s) 406
Csp6I GTAC 3 cut(s) 289, 431, 479
CviAII CATG 3 cut(s) 138, 483, 504
CviQI GTAC 3 cut(s) 289, 431, 479
DdeI CTNAG 3 cut(s) 177, 515, 532
DpnI GATC 1 cut(s) 306
DpnII GATC 1 cut(s) 304
EaeI YGGCCR 2 cut(s) 30, 212
EagI CGGCCG 1 cut(s) 30
Ecl136II GAGCTC 2 cut(s) 135, 284
EclXI CGGCCG 1 cut(s) 30
Eco130I CCWWGG 2 cut(s) 192, 482
Eco24I GRGCYC 3 cut(s) 137, 286, 346
Eco31I GGTCTC 3 cut(s) 98, 125, 175
Eco47I GGWCC 1 cut(s) 406
Eco52I CGGCCG 1 cut(s) 30
Eco53kI GAGCTC 2 cut(s) 135, 284
Eco81I CCTNAGG 1 cut(s) 515
EcoICRI GAGCTC 2 cut(s) 135, 284
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoRII CCWGG 2 cut(s) 63, 73
EcoT14I CCWWGG 2 cut(s) 192, 482
EcoT38I GRGCYC 3 cut(s) 137, 286, 346
ErhI CCWWGG 2 cut(s) 192, 482
Esp3I CGTCTC 2 cut(s) 44, 305
FaeI CATG 3 cut(s) 141, 486, 507
FaiI YATR 8 cut(s) 139, 261, 404, 429, 441, 484, 491, 505
FatI CATG 3 cut(s) 137, 482, 503
Fnu4HI GCNGC 5 cut(s) 215, 253, 332, 580, 583
FokI GGATG 2 cut(s) 359, 475
FriOI GRGCYC 3 cut(s) 137, 286, 346
Fsp4HI GCNGC 5 cut(s) 215, 253, 332, 580, 583
FspBI CTAG 2 cut(s) 231, 347
FspI TGCGCA 1 cut(s) 296
GlaI GCGC 2 cut(s) 296, 512
GluI GCNGC 5 cut(s) 215, 253, 332, 580, 583
GsuI CTGGAG 1 cut(s) 171
HaeII RGCGCY 1 cut(s) 514
HaeIII GGCC 2 cut(s) 32, 214
HapII CCGG 2 cut(s) 8, 29
HhaI GCGC 2 cut(s) 297, 513
Hin1II CATG 3 cut(s) 141, 486, 507
Hin6I GCGC 2 cut(s) 295, 511
HinP1I GCGC 2 cut(s) 295, 511
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HindIII AAGCTT 1 cut(s) 398
HinfI GANTC 1 cut(s) 500
HpaII CCGG 2 cut(s) 8, 29
Hpy166II GTNNAC 1 cut(s) 150
Hpy188I TCNGA 4 cut(s) 103, 180, 535, 555
Hpy188III TCNNGA 3 cut(s) 308, 347, 367
Hpy8I GTNNAC 1 cut(s) 150
Hpy99I CGWCG 4 cut(s) 50, 116, 119, 148
HpyAV CCTTC 3 cut(s) 259, 373, 573
HpyCH4III ACNGT 3 cut(s) 24, 112, 157
HpyCH4IV ACGT 2 cut(s) 91, 314
HpyCH4V TGCA 6 cut(s) 19, 255, 272, 318, 507, 573
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 579
HpyF3I CTNAG 3 cut(s) 177, 515, 532
HpySE526I ACGT 2 cut(s) 91, 314
Hsp92II CATG 3 cut(s) 141, 486, 507
HspAI GCGC 2 cut(s) 295, 511
KroI GCCGGC 1 cut(s) 28
KroNI GCCGGC 1 cut(s) 30
Kzo9I GATC 1 cut(s) 304
LmnI GCTCC 4 cut(s) 132, 281, 289, 556
LpnPI CCDG 9 cut(s) 21, 42, 50, 60, 66, 77, 87, 201, 231
Lsp1109I GCAGC 2 cut(s) 239, 343
LweI GCATC 1 cut(s) 453
MaeI CTAG 2 cut(s) 231, 347
MaeII ACGT 2 cut(s) 91, 314
MaeIII GTNAC 1 cut(s) 3
MalI GATC 1 cut(s) 306
MboI GATC 1 cut(s) 304
MflI RGATCY 1 cut(s) 304
MhlI GDGCHC 3 cut(s) 137, 286, 346
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 1 cut(s) 451
MnlI CCTC 6 cut(s) 195, 218, 228, 343, 436, 529
MroNI GCCGGC 1 cut(s) 28
MroXI GAANNNNTTC 1 cut(s) 562
MseI TTAA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 585
MspI CCGG 2 cut(s) 8, 29
MspR9I CCNGG 2 cut(s) 65, 75
MvaI CCWGG 2 cut(s) 65, 75
MwoI GCNNNNNNNGC 2 cut(s) 25, 579
NaeI GCCGGC 1 cut(s) 30
NcoI CCATGG 1 cut(s) 482
NdeII GATC 1 cut(s) 304
NgoMIV GCCGGC 1 cut(s) 28
NlaIII CATG 3 cut(s) 141, 486, 507
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 1 cut(s) 3
NsbI TGCGCA 1 cut(s) 296
PasI CCCWGGG 1 cut(s) 64
PdiI GCCGGC 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 562
PkrI GCNGC 5 cut(s) 216, 254, 333, 581, 584
PleI GAGTC 1 cut(s) 508
PpsI GAGTC 1 cut(s) 508
PshAI GACNNNNGTC 1 cut(s) 110
Psp124BI GAGCTC 2 cut(s) 137, 286
Psp6I CCWGG 2 cut(s) 63, 73
PspGI CCWGG 2 cut(s) 63, 73
PspPI GGNCC 1 cut(s) 406
PstNI CAGNNNCTG 1 cut(s) 585
PsuI RGATCY 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 585
RsaI GTAC 3 cut(s) 290, 432, 480
RsaNI GTAC 3 cut(s) 289, 431, 479
SacI GAGCTC 2 cut(s) 137, 286
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 5 cut(s) 215, 253, 332, 580, 583
Sau3AI GATC 1 cut(s) 304
Sau96I GGNCC 1 cut(s) 406
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 2 cut(s) 65, 75
SduI GDGCHC 3 cut(s) 137, 286, 346
SfaNI GCATC 1 cut(s) 453
SinI GGWCC 1 cut(s) 406
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SsiI CCGC 1 cut(s) 215
SspMI CTAG 2 cut(s) 231, 347
SstI GAGCTC 2 cut(s) 137, 286
StyD4I CCNGG 2 cut(s) 63, 73
StyI CCWWGG 2 cut(s) 192, 482
TaaI ACNGT 3 cut(s) 24, 112, 157
TaiI ACGT 2 cut(s) 94, 317
TaqII GACCGA 1 cut(s) 107
TauI GCSGC 1 cut(s) 217
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 4 cut(s) 252, 331, 579, 582
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 539
TspGWI ACGGA 2 cut(s) 276, 365
VpaK11BI GGWCC 1 cut(s) 406
XagI CCTNNNNNAGG 1 cut(s) 222
XbaI TCTAGA 1 cut(s) 346
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 562
XspI CTAG 2 cut(s) 231, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.