RLG00000019823

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014145
Physical Location & Seq
Reverse (-)
57124434 .. 57127244
2811 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019823

Sequence Viewer

Length: 1014 bp
ATGGAGAGCGCCGGAGAGTATCATATCGGCCCCGACGAGTCGTACCGGTCTCTGCCGTCGGTTTACTTGGCCTTCTTGACAATCTGGTTCATCTCCGCCTGCTCCTGGACCTTCAACACCTACAAAAACCGACACCTCCAGTCGAATAATTTGCAGTGGACGCTCACATCTGTACCATTAATTAAAGCCTTGCAACTCACATTGTCTTTCCTCTTCTGGTATTCATGCCTTCATCTACAGATATGCTCTCTGTGGATGTCATTTGGGGTGTATGTAACTGGAGTGATATTTCAAACGGCTTCTTTTGTGTCCTTTTTACTCATATCTCATGGTTACTGCATCATGTGCGAGCGTCTTTCAGTGACCGAGCGGCGTACTACTGCTTCCCTTGGCTGTGTGTTTTACTTGACTCTAGTTGGATACAGAGCTTCTGTACCTTATTTTTCTGTCCTCCTGCTGCTTAACTATTTTATCTCATTCTACATGATATTCCACCATATATCTCAAAATCTGTTAGTATTGCGGGAACAGTTAAATTTCATAGATAATGAGGACGTGCATGCAATGCATGATGCAGTCTATACAAAGTACGTCATGTTCAAAAAATTCCAAGGTGCAATGCAGATAGTAGCCATGGCAGAGACTGTGATATACATTAACATGGATGACTCATCAGAAAATTACTGGATTCGATTATTAGTTCGAGAGTGGGCACAGTTCTGCATCTTTGTTTACATAGGGTGGACTTTTAGGTCAAGGGACTTGGCACCACGTTTTTCAGTTATGCCAACTTTGAAATCTAAAGGGGAACTAGCAGTCGCTCCCATTTATAGCATTGAAATGGATGCAGAAACTTTCAGAGATTTTACTCGGCATGAATGGCACATTGGTGTGCCAACTGTAAAATCTAGTGATGAGAGCTCAAAGGATTCTGTCTTGGTAATAATTCAGCATCCTCGTGCATTTAGGCTGACCGAAGCTGGAGCTGGAAAATCTTCTCAAAGCCAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lung_7-TM_R

Protein Analysis

338

Amino Acids

38.98

Weight (kDa)

6.82

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52155
fragaria_vesca FvH4_6g32020
malus_domestica MD09G1206800.v1.1 MD17G1187500.v1.1
prunus_persica Prupe.3G062900_v2.0.a1 Prupe.3G062900_v2.0.a1
pyrus_communis pycom09g12320 pycom17g19650
rosa_chinensis RchiOBHm_Chr2g0139631
rosa_laevigata RLG00000019823
rosa_multiflora Rmu_sc0009449.1_g000005
rosa_roxburghii Rroxscaffold_2G00105330
rosa_rugosa Rorug02G0358800
rosa_samantha Rh2AG408800 Rh2CG394700 Rh2DG428400
rosa_wichuraiana Rw2G033490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 751
AccB1I GGYRCC 1 cut(s) 766
AccBSI CCGCTC 1 cut(s) 370
AciI CCGC 3 cut(s) 96, 370, 523
AcsI RAATTY 2 cut(s) 535, 605
AfaI GTAC 5 cut(s) 44, 174, 376, 435, 590
AfiI CCNNNNNNNGG 1 cut(s) 105
AgeI ACCGGT 1 cut(s) 45
AgsI TTSAA 5 cut(s) 115, 293, 601, 796, 839
AjiI CACGTC 1 cut(s) 556
AjnI CCWGG 1 cut(s) 104
AjuI GAANNNNNNNTTGG 2 cut(s) 870, 902
AleI CACNNNNGTG 1 cut(s) 888
AluBI AGCT 5 cut(s) 428, 921, 980, 986, 1010
AluI AGCT 5 cut(s) 428, 921, 980, 986, 1010
Alw21I GWGCWC 1 cut(s) 923
Alw26I GTCTC 2 cut(s) 54, 635
AlwNI CAGNNNCTG 1 cut(s) 644
AoxI GGCC 2 cut(s) 28, 69
ApeKI GCWGC 1 cut(s) 457
ApoI RAATTY 2 cut(s) 535, 605
AseI ATTAAT 1 cut(s) 179
AsiGI ACCGGT 1 cut(s) 45
Asp700I GAANNNNTTC 1 cut(s) 994
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 29, 108
AvaII GGWCC 1 cut(s) 108
BaeGI GKGCMC 1 cut(s) 715
BaeI ACNNNNGTAYC 2 cut(s) 156, 189
BanI GGYRCC 1 cut(s) 766
BanII GRGCYC 1 cut(s) 923
BarI GAAGNNNNNNTAC 2 cut(s) 367, 399
BauI CACGAG 1 cut(s) 957
Bbv12I GWGCWC 1 cut(s) 923
BbvI GCAGC 1 cut(s) 444
BceAI ACGGC 2 cut(s) 40, 312
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 1 cut(s) 106
BciVI GTATCC 1 cut(s) 413
BcoDI GTCTC 2 cut(s) 54, 635
BfaI CTAG 3 cut(s) 413, 812, 909
BfmI CTRYAG 1 cut(s) 236
BfoI RGCGCY 1 cut(s) 12
BfuI GTATCC 1 cut(s) 413
BisI GCNGC 2 cut(s) 371, 458
BlsI GCNGC 2 cut(s) 372, 459
Bme1390I CCNGG 1 cut(s) 106
Bme18I GGWCC 1 cut(s) 108
BmgBI CACGTC 1 cut(s) 556
BmgT120I GGNCC 2 cut(s) 29, 108
BmiI GGNNCC 2 cut(s) 31, 768
BmrFI CCNGG 1 cut(s) 106
BmsI GCATC 5 cut(s) 348, 562, 732, 835, 961
BpmI CTGGAG 3 cut(s) 122, 300, 1002
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 388, 610, 633
BsaWI WCCGGW 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 3 cut(s) 139, 283, 689
Bse3DI GCAATG 2 cut(s) 570, 624
BseBI CCWGG 1 cut(s) 106
BseDI CCNNGG 3 cut(s) 388, 610, 633
BseGI GGATG 4 cut(s) 261, 670, 850, 952
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMI GCAATG 2 cut(s) 570, 624
BseNI ACTGG 3 cut(s) 139, 283, 689
BseSI GKGCMC 1 cut(s) 715
BseXI GCAGC 1 cut(s) 444
BshFI GGCC 2 cut(s) 30, 71
BshNI GGYRCC 1 cut(s) 766
BshTI ACCGGT 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 923
BsiSI CCGG 2 cut(s) 12, 46
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 2 cut(s) 54, 635
BsmFI GGGAC 1 cut(s) 773
BsnI GGCC 2 cut(s) 30, 71
Bso31I GGTCTC 1 cut(s) 54
Bsp1286I GDGCHC 2 cut(s) 715, 923
Bsp19I CCATGG 1 cut(s) 633
BspACI CCGC 3 cut(s) 96, 370, 523
BspANI GGCC 2 cut(s) 30, 71
BspLI GGNNCC 2 cut(s) 31, 768
BspT107I GGYRCC 1 cut(s) 766
BspTNI GGTCTC 1 cut(s) 54
BsrBI CCGCTC 1 cut(s) 370
BsrDI GCAATG 2 cut(s) 570, 624
BsrFI RCCGGY 1 cut(s) 45
BsrI ACTGG 3 cut(s) 139, 283, 689
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 3 cut(s) 388, 610, 633
BssSI CACGAG 1 cut(s) 957
BssT1I CCWWGG 3 cut(s) 388, 610, 633
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 4 cut(s) 531, 646, 717, 901
Bst6I CTCTTC 1 cut(s) 218
BstAPI GCANNNNNTGC 2 cut(s) 345, 565
BstC8I GCNNGC 3 cut(s) 100, 350, 561
BstDEI CTNAG 1 cut(s) 1011
BstDSI CCRYGG 1 cut(s) 633
BstF5I GGATG 4 cut(s) 261, 670, 850, 952
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstMAI GTCTC 2 cut(s) 54, 635
BstMWI GCNNNNNNNGC 4 cut(s) 160, 345, 565, 880
BstNI CCWGG 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 236
BstSLI GKGCMC 1 cut(s) 715
BstV1I GCAGC 1 cut(s) 444
BsuI GTATCC 1 cut(s) 413
BsuRI GGCC 2 cut(s) 30, 71
BtgI CCRYGG 1 cut(s) 633
BtrI CACGTC 1 cut(s) 556
BtsCI GGATG 4 cut(s) 261, 670, 850, 952
BtsI GCAGTG 1 cut(s) 161
BtsIMutI CAGTG 2 cut(s) 161, 366
Cac8I GCNNGC 3 cut(s) 100, 350, 561
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 1 cut(s) 11
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 2 cut(s) 29, 108
CseI GACGC 2 cut(s) 169, 341
Csp6I GTAC 5 cut(s) 43, 173, 375, 434, 589
CspAI ACCGGT 1 cut(s) 45
CviQI GTAC 5 cut(s) 43, 173, 375, 434, 589
DdeI CTNAG 1 cut(s) 1011
DrdI GACNNNNNNGTC 1 cut(s) 751
DseDI GACNNNNNNGTC 1 cut(s) 751
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 921
Eco130I CCWWGG 3 cut(s) 388, 610, 633
Eco24I GRGCYC 1 cut(s) 923
Eco31I GGTCTC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 921
EcoICRI GAGCTC 1 cut(s) 921
EcoRII CCWGG 1 cut(s) 104
EcoT14I CCWWGG 3 cut(s) 388, 610, 633
EcoT22I ATGCAT 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 923
ErhI CCWWGG 3 cut(s) 388, 610, 633
FalI AAGNNNNNCTT 1 cut(s) 994
FaqI GGGAC 1 cut(s) 773
FauI CCCGC 1 cut(s) 516
Fnu4HI GCNGC 2 cut(s) 371, 458
FokI GGATG 4 cut(s) 268, 677, 857, 939
FriOI GRGCYC 1 cut(s) 923
Fsp4HI GCNGC 2 cut(s) 371, 458
FspBI CTAG 3 cut(s) 413, 812, 909
GlaI GCGC 1 cut(s) 10
GluI GCNGC 2 cut(s) 371, 458
GsuI CTGGAG 3 cut(s) 122, 300, 1002
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 30, 71
HapII CCGG 2 cut(s) 12, 46
HgaI GACGC 2 cut(s) 169, 341
HhaI GCGC 1 cut(s) 11
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HindIII AAGCTT 1 cut(s) 1008
HinfI GANTC 5 cut(s) 38, 409, 668, 688, 929
HpaII CCGG 2 cut(s) 12, 46
Hpy166II GTNNAC 4 cut(s) 64, 159, 733, 744
Hpy188I TCNGA 2 cut(s) 676, 860
Hpy188III TCNNGA 2 cut(s) 76, 704
Hpy8I GTNNAC 4 cut(s) 64, 159, 733, 744
Hpy99I CGWCG 2 cut(s) 38, 61
HpyAV CCTTC 3 cut(s) 82, 121, 239
HpyCH4III ACNGT 4 cut(s) 531, 646, 717, 901
HpyCH4IV ACGT 3 cut(s) 555, 591, 772
HpyF10VI GCNNNNNNNGC 4 cut(s) 160, 345, 565, 880
HpyF3I CTNAG 1 cut(s) 1011
HpySE526I ACGT 3 cut(s) 555, 591, 772
HspAI GCGC 1 cut(s) 9
LmnI GCTCC 3 cut(s) 107, 826, 983
Lsp1109I GCAGC 1 cut(s) 444
LweI GCATC 5 cut(s) 348, 562, 732, 835, 961
MaeI CTAG 3 cut(s) 413, 812, 909
MaeII ACGT 3 cut(s) 555, 591, 772
MaeIII GTNAC 3 cut(s) 274, 332, 361
MbiI CCGCTC 1 cut(s) 370
MboII GAAGA 2 cut(s) 205, 987
MhlI GDGCHC 2 cut(s) 715, 923
MluCI AATT 6 cut(s) 148, 180, 535, 605, 679, 945
MlyI GAGTC 3 cut(s) 47, 403, 662
MmeI TCCRAC 1 cut(s) 397
MnlI CCTC 5 cut(s) 146, 221, 461, 544, 966
Mph1103I ATGCAT 1 cut(s) 570
MroXI GAANNNNTTC 1 cut(s) 994
MseI TTAA 5 cut(s) 179, 183, 462, 533, 657
MslI CAYNNNNRTG 5 cut(s) 659, 839, 888, 890, 957
MspI CCGG 2 cut(s) 12, 46
MspR9I CCNGG 1 cut(s) 106
MvaI CCWGG 1 cut(s) 106
MwoI GCNNNNNNNGC 4 cut(s) 160, 345, 565, 880
NcoI CCATGG 1 cut(s) 633
NlaIV GGNNCC 2 cut(s) 31, 768
NmeAIII GCCGAG 1 cut(s) 850
NmuCI GTSAC 1 cut(s) 361
NsiI ATGCAT 1 cut(s) 570
NspI RCATGY 1 cut(s) 563
OliI CACNNNNGTG 1 cut(s) 888
PacI TTAATTAA 1 cut(s) 183
PaeI GCATGC 1 cut(s) 563
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 994
PfeI GAWTC 2 cut(s) 688, 929
PfoI TCCNGGA 1 cut(s) 104
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 2 cut(s) 372, 459
PleI GAGTC 3 cut(s) 46, 403, 662
PpsI GAGTC 3 cut(s) 46, 403, 662
PshBI ATTAAT 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 923
Psp6I CCWGG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 2 cut(s) 31, 768
PspPI GGNCC 2 cut(s) 29, 108
PsrI GAACNNNNNNTAC 2 cut(s) 581, 613
PstNI CAGNNNCTG 1 cut(s) 644
RsaI GTAC 5 cut(s) 44, 174, 376, 435, 590
RsaNI GTAC 5 cut(s) 43, 173, 375, 434, 589
RseI CAYNNNNRTG 5 cut(s) 659, 839, 888, 890, 957
SacI GAGCTC 1 cut(s) 923
SaqAI TTAA 5 cut(s) 179, 183, 462, 533, 657
SatI GCNGC 2 cut(s) 371, 458
Sau96I GGNCC 2 cut(s) 29, 108
SchI GAGTC 3 cut(s) 47, 403, 662
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 2 cut(s) 715, 923
SfaNI GCATC 5 cut(s) 348, 562, 732, 835, 961
SfcI CTRYAG 1 cut(s) 236
SinI GGWCC 1 cut(s) 108
SmiMI CAYNNNNRTG 5 cut(s) 659, 839, 888, 890, 957
SphI GCATGC 1 cut(s) 563
Sse9I AATT 6 cut(s) 148, 180, 535, 605, 679, 945
SsiI CCGC 3 cut(s) 96, 370, 523
SspMI CTAG 3 cut(s) 413, 812, 909
SstI GAGCTC 1 cut(s) 923
StyD4I CCNGG 1 cut(s) 104
StyI CCWWGG 3 cut(s) 388, 610, 633
TaaI ACNGT 4 cut(s) 531, 646, 717, 901
TaiI ACGT 3 cut(s) 558, 594, 775
TaqI TCGA 3 cut(s) 143, 691, 703
TaqII GACCGA 2 cut(s) 380, 989
TasI AATT 6 cut(s) 148, 180, 535, 605, 679, 945
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 2 cut(s) 688, 929
Tru1I TTAA 5 cut(s) 179, 183, 462, 533, 657
Tru9I TTAA 5 cut(s) 179, 183, 462, 533, 657
TscAI CASTG 2 cut(s) 161, 366
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 1 cut(s) 457
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 5 cut(s) 79, 213, 221, 529, 891
TspRI CASTG 2 cut(s) 161, 366
VpaK11BI GGWCC 1 cut(s) 108
VspI ATTAAT 1 cut(s) 179
XapI RAATTY 2 cut(s) 535, 605
XceI RCATGY 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 994
XspI CTAG 3 cut(s) 413, 812, 909
Zsp2I ATGCAT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.