RLG00000020037

Haloacid dehalogenase-like hydrolase domain-containing protein At3g48420

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015751
Physical Location & Seq
Forward (+)
60362605 .. 60363822
1218 bp
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UTR
Exon/CDS
Intron
RLM00000020037

Sequence Viewer

Length: 375 bp
ATGGTGGTTGTTCCAGAGTTCATGGCCCCCGTTTCGGGGACGAATAGGAGCAACTCCGAAAGAAGTGAGCTTCGAGTTTTTGAGGCCTCAGTCCTTTTCAACCATGTTCCACGATACCAACTCTCACCTCTATGCTTTACCCAAGAACCATCTCCACTTGCCCTCTCAATTTTCCCCAGCTTTCCCAAAAAACTCAACCTTTCCCGGAAAATGTCTCCACTTTCATGCTTCACTGCTTCCAGTTTATTTTCCGGCGCCGACGACCAGAACCCATCTCAGGAACTTGCTTTTCTTCTTGAAGTTGACAGGGTTCTTATGGATGCATATCATTTGGGTAACCTCCCAACCTTCAATTTAGGTGCACAGATGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.8

Weight (kDa)

5.58

Isoelectric Point (pI)

60.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 255
AfiI CCNNNNNNNGG 4 cut(s) 34, 35, 36, 277
AgsI TTSAA 3 cut(s) 100, 299, 352
AluBI AGCT 2 cut(s) 70, 180
AluI AGCT 2 cut(s) 70, 180
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 1 cut(s) 219
Alw44I GTGCAC 1 cut(s) 360
AoxI GGCC 2 cut(s) 24, 84
ApaLI GTGCAC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 25
AsuC2I CCSGG 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 364
BanI GGYRCC 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 364
BccI CCATC 2 cut(s) 157, 280
BcnI CCSGG 1 cut(s) 205
BcoDI GTCTC 1 cut(s) 219
BfoI RGCGCY 1 cut(s) 258
Bme1390I CCNGG 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 2 cut(s) 27, 256
BmrFI CCNGG 1 cut(s) 205
BmsI GCATC 2 cut(s) 310, 357
BpuMI CCSGG 1 cut(s) 205
BsaBI GATNNNNATC 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 255
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 35, 36, 277
Bse1I ACTGG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 324
BseGI GGATG 1 cut(s) 325
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 4 cut(s) 34, 35, 36, 277
BseMII CTCAG 2 cut(s) 102, 290
BseNI ACTGG 1 cut(s) 240
BseSI GKGCMC 1 cut(s) 364
BseYI CCCAGC 1 cut(s) 176
BshFI GGCC 2 cut(s) 26, 86
BshNI GGYRCC 1 cut(s) 254
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 2 cut(s) 205, 252
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 4 cut(s) 34, 35, 36, 277
BsmAI GTCTC 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 52
BsnI GGCC 2 cut(s) 26, 86
Bsp1286I GDGCHC 1 cut(s) 364
BspANI GGCC 2 cut(s) 26, 86
BspCNI CTCAG 2 cut(s) 101, 289
BspLI GGNNCC 2 cut(s) 27, 256
BspT107I GGYRCC 1 cut(s) 254
BsrI ACTGG 1 cut(s) 240
BssNI GRCGYC 1 cut(s) 255
BstACI GRCGYC 1 cut(s) 255
BstDEI CTNAG 2 cut(s) 88, 276
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 1 cut(s) 325
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 1 cut(s) 257
BstMAI GTCTC 1 cut(s) 219
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 203
BstSLI GKGCMC 1 cut(s) 364
BsuRI GGCC 2 cut(s) 26, 86
BtsCI GGATG 1 cut(s) 325
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 231
CfoI GCGC 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 25
CviAII CATG 3 cut(s) 22, 104, 225
CviJI RGCY 4 cut(s) 26, 70, 86, 180
CviKI_1 RGCY 4 cut(s) 26, 70, 86, 180
DdeI CTNAG 2 cut(s) 88, 276
DinI GGCGCC 1 cut(s) 256
Eco147I AGGCCT 1 cut(s) 86
Eco91I GGTNACC 1 cut(s) 335
EcoO65I GGTNACC 1 cut(s) 335
EcoT22I ATGCAT 1 cut(s) 325
EgeI GGCGCC 1 cut(s) 256
EheI GGCGCC 1 cut(s) 256
FaeI CATG 3 cut(s) 25, 107, 228
FaiI YATR 6 cut(s) 23, 105, 133, 226, 317, 325
FaqI GGGAC 1 cut(s) 52
FatI CATG 3 cut(s) 21, 103, 224
FokI GGATG 1 cut(s) 332
GlaI GCGC 1 cut(s) 256
GsaI CCCAGC 1 cut(s) 180
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 2 cut(s) 26, 86
HapII CCGG 2 cut(s) 205, 252
HhaI GCGC 1 cut(s) 257
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 3 cut(s) 25, 107, 228
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HpaII CCGG 2 cut(s) 205, 252
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 2 cut(s) 304, 362
Hpy188I TCNGA 1 cut(s) 58
Hpy188III TCNNGA 3 cut(s) 14, 278, 296
Hpy8I GTNNAC 2 cut(s) 304, 362
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 1 cut(s) 358
HpyCH4V TGCA 3 cut(s) 323, 362, 370
HpyF3I CTNAG 2 cut(s) 88, 276
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 3 cut(s) 25, 107, 228
HspAI GCGC 1 cut(s) 255
KasI GGCGCC 1 cut(s) 254
LmnI GCTCC 1 cut(s) 48
LpnPI CCDG 8 cut(s) 27, 190, 218, 253, 263, 265, 278, 292
LweI GCATC 2 cut(s) 310, 357
MaeIII GTNAC 1 cut(s) 335
MboII GAAGA 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 2 cut(s) 168, 352
Mly113I GGCGCC 1 cut(s) 255
MnlI CCTC 5 cut(s) 76, 97, 138, 173, 350
Mph1103I ATGCAT 1 cut(s) 325
MslI CAYNNNNRTG 2 cut(s) 130, 223
MspI CCGG 2 cut(s) 205, 252
MspR9I CCNGG 1 cut(s) 205
NarI GGCGCC 1 cut(s) 255
NciI CCSGG 1 cut(s) 205
NlaIII CATG 3 cut(s) 25, 107, 228
NlaIV GGNNCC 2 cut(s) 27, 256
NsiI ATGCAT 1 cut(s) 325
PceI AGGCCT 1 cut(s) 86
PfoI TCCNGGA 1 cut(s) 203
PluTI GGCGCC 1 cut(s) 258
PspEI GGTNACC 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 176
PspN4I GGNNCC 2 cut(s) 27, 256
PspPI GGNCC 1 cut(s) 25
RseI CAYNNNNRTG 2 cut(s) 130, 223
Sau96I GGNCC 1 cut(s) 25
ScrFI CCNGG 1 cut(s) 205
SduI GDGCHC 1 cut(s) 364
SetI ASST 7 cut(s) 72, 130, 182, 201, 342, 350, 361
SfaNI GCATC 2 cut(s) 310, 357
SfoI GGCGCC 1 cut(s) 256
SmiMI CAYNNNNRTG 2 cut(s) 130, 223
Sse9I AATT 2 cut(s) 168, 352
SseBI AGGCCT 1 cut(s) 86
SspDI GGCGCC 1 cut(s) 254
StuI AGGCCT 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 203
TaqI TCGA 1 cut(s) 73
TasI AATT 2 cut(s) 168, 352
TscAI CASTG 1 cut(s) 238
TspDTI ATGAA 2 cut(s) 10, 213
TspRI CASTG 1 cut(s) 238
VneI GTGCAC 1 cut(s) 360
Zsp2I ATGCAT 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.