RLG00000020479

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014774
Physical Location & Seq
Forward (+)
65479662 .. 65480465
804 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020479

Sequence Viewer

Length: 804 bp
ATGAGGCAATGGGGTTGGCTTCTTGGGACACTAACAATGCTGCAGGTTCTGGTTTTTCTCTTGGTTGGTTATGGATTTGACAACAGCATCCCAGCAAAAAGATCATTGCTGAGTGATTCTGGACGACATTATGGGGTGAAGATGGTAAATACATCTGACAGCATTGTGTTGGATAATGGCCTTGTCCAACTAACGTTCTCTAATCCCGCTGGAGACGTTATTGGAATCAGATATGGGGGAATTGATAACTTGCTTGAAATCCACAATAAGCCAACTAATAGAGGTTACTGGGATTTGAACTGGAACAATCTGGGAGAGAAGAGCAGTATCTTTGAAGGAATATTGGGAACAGAATTTCGAGTGATCACAGCTATGGAAGACCAAATAGAAATCTCTTTCACCAGAACATACAATGTTTCCCTTCCCCAGGGTGTGACAGTTCCATTTAATGTGGGCAAAATGTACATATTGCAGCGTGATCATTCTGGGTTCTATGCATATGCCATTTTTGAGCGCTTAGAAGGGTGGCCCGAAGTGGTGATTTATCAAATAAGAATGGTTTTCAAACTTCAACAAGCCAAGTTCCGGTACATGGCGCTATCGGACACTAGACAAAGGTTCATGCCAACAGCCTATGAACGTGGATATTATGGTATGAAACTCGCCTATAAAGAAGCTGTTCTCGTAAACAATACAGGAGAGGTAGATGACAAGTATCAGTACTCAATTGAGGACAAGGATAACAAGGTGCATGGTTGGATTTGTACTGATGACGAATCGGTTTTGGATGATCACACCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

268

Amino Acids

30.69

Weight (kDa)

5.59

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 42 - 232 4.2e-71 Rhamnogalacturonate lyase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 34
AciI CCGC 1 cut(s) 207
AclI AACGTT 1 cut(s) 194
AcsI RAATTY 1 cut(s) 353
AdeI CACNNNGTG 1 cut(s) 801
AfaI GTAC 4 cut(s) 464, 590, 722, 766
AfeI AGCGCT 1 cut(s) 515
AfiI CCNNNNNNNGG 3 cut(s) 427, 585, 592
AgsI TTSAA 5 cut(s) 257, 298, 335, 565, 572
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 2 cut(s) 371, 677
AluI AGCT 2 cut(s) 371, 677
Alw26I GTCTC 1 cut(s) 207
AlwNI CAGNNNCTG 1 cut(s) 49
Aor51HI AGCGCT 1 cut(s) 515
AoxI GGCC 2 cut(s) 178, 527
ApeKI GCWGC 2 cut(s) 40, 472
ApoI RAATTY 1 cut(s) 353
Asp700I GAANNNNTTC 1 cut(s) 678
AspLEI GCGC 2 cut(s) 516, 598
AspS9I GGNCC 1 cut(s) 528
AsuHPI GGTGA 3 cut(s) 148, 391, 550
BbsI GAAGAC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 27, 484
BccI CCATC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 428
BclI TGATCA 3 cut(s) 363, 478, 790
BcoDI GTCTC 1 cut(s) 207
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 1 cut(s) 41
BfoI RGCGCY 2 cut(s) 517, 599
BfuAI ACCTGC 1 cut(s) 34
BisI GCNGC 2 cut(s) 41, 473
BlsI GCNGC 2 cut(s) 42, 474
BmcAI AGTACT 1 cut(s) 722
Bme1390I CCNGG 1 cut(s) 428
BmgT120I GGNCC 1 cut(s) 528
BmrFI CCNGG 1 cut(s) 428
BmrI ACTGGG 1 cut(s) 298
BmsI GCATC 1 cut(s) 96
BmuI ACTGGG 1 cut(s) 298
BpiI GAAGAC 1 cut(s) 384
BpmI CTGGAG 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 426, 427
BsaWI WCCGGW 1 cut(s) 585
Bsc4I CCNNNNNNNGG 3 cut(s) 427, 585, 592
Bse1I ACTGG 2 cut(s) 293, 305
Bse3DI GCAATG 2 cut(s) 14, 104
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 2 cut(s) 426, 427
BseGI GGATG 2 cut(s) 87, 793
BseLI CCNNNNNNNGG 3 cut(s) 427, 585, 592
BseMI GCAATG 2 cut(s) 14, 104
BseMII CTCAG 1 cut(s) 101
BseNI ACTGG 2 cut(s) 293, 305
BseXI GCAGC 2 cut(s) 27, 484
BseYI CCCAGC 1 cut(s) 91
BshFI GGCC 2 cut(s) 180, 529
BsiSI CCGG 1 cut(s) 586
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 3 cut(s) 427, 585, 592
BsmAI GTCTC 1 cut(s) 207
BsmBI CGTCTC 1 cut(s) 207
BsmFI GGGAC 1 cut(s) 40
BsnI GGCC 2 cut(s) 180, 529
Bsp1407I TGTACA 1 cut(s) 462
Bsp143I GATC 4 cut(s) 101, 363, 478, 790
BspACI CCGC 1 cut(s) 207
BspANI GGCC 2 cut(s) 180, 529
BspCNI CTCAG 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 45
BspMI ACCTGC 1 cut(s) 34
BspQI GCTCTTC 1 cut(s) 314
BsrDI GCAATG 2 cut(s) 14, 104
BsrGI TGTACA 1 cut(s) 462
BsrI ACTGG 2 cut(s) 293, 305
BssECI CCNNGG 2 cut(s) 426, 427
BssMI GATC 4 cut(s) 101, 363, 478, 790
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 439
Bst6I CTCTTC 1 cut(s) 314
BstAUI TGTACA 1 cut(s) 462
BstDEI CTNAG 2 cut(s) 110, 517
BstENI CCTNNNNNAGG 1 cut(s) 425
BstF5I GGATG 2 cut(s) 87, 793
BstH2I RGCGCY 2 cut(s) 517, 599
BstHHI GCGC 2 cut(s) 516, 598
BstKTI GATC 4 cut(s) 104, 366, 481, 793
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 4 cut(s) 101, 363, 478, 790
BstNI CCWGG 1 cut(s) 428
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 1 cut(s) 41
BstV1I GCAGC 2 cut(s) 27, 484
BstV2I GAAGAC 1 cut(s) 384
BsuRI GGCC 2 cut(s) 180, 529
BtsCI GGATG 2 cut(s) 87, 793
BveI ACCTGC 1 cut(s) 34
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 2 cut(s) 516, 598
Cfr13I GGNCC 1 cut(s) 528
Csp6I GTAC 4 cut(s) 463, 589, 721, 765
CviAII CATG 3 cut(s) 592, 622, 752
CviJI RGCY 8 cut(s) 19, 180, 271, 371, 529, 578, 632, 677
CviKI_1 RGCY 8 cut(s) 19, 180, 271, 371, 529, 578, 632, 677
CviQI GTAC 4 cut(s) 463, 589, 721, 765
DdeI CTNAG 2 cut(s) 110, 517
DpnI GATC 4 cut(s) 103, 365, 480, 792
DpnII GATC 4 cut(s) 101, 363, 478, 790
DraIII CACNNNGTG 1 cut(s) 801
Eam1104I CTCTTC 1 cut(s) 314
EarI CTCTTC 1 cut(s) 314
Eco47III AGCGCT 1 cut(s) 515
EcoNI CCTNNNNNAGG 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 499
Esp3I CGTCTC 1 cut(s) 207
FaeI CATG 3 cut(s) 595, 625, 755
FaqI GGGAC 1 cut(s) 40
FatI CATG 3 cut(s) 591, 621, 751
FauI CCCGC 1 cut(s) 214
FauNDI CATATG 1 cut(s) 499
FbaI TGATCA 3 cut(s) 363, 478, 790
Fnu4HI GCNGC 2 cut(s) 41, 473
FokI GGATG 2 cut(s) 74, 800
Fsp4HI GCNGC 2 cut(s) 41, 473
FspBI CTAG 1 cut(s) 609
GlaI GCGC 2 cut(s) 515, 597
GluI GCNGC 2 cut(s) 41, 473
GsaI CCCAGC 1 cut(s) 95
GsuI CTGGAG 1 cut(s) 231
HaeII RGCGCY 2 cut(s) 517, 599
HaeIII GGCC 2 cut(s) 180, 529
HapII CCGG 1 cut(s) 586
HhaI GCGC 2 cut(s) 516, 598
Hin1II CATG 3 cut(s) 595, 625, 755
Hin6I GCGC 2 cut(s) 514, 596
HinP1I GCGC 2 cut(s) 514, 596
HinfI GANTC 3 cut(s) 116, 225, 776
HpaII CCGG 1 cut(s) 586
HphI GGTGA 3 cut(s) 148, 391, 550
Hpy166II GTNNAC 1 cut(s) 688
Hpy188I TCNGA 3 cut(s) 157, 230, 604
Hpy188III TCNNGA 1 cut(s) 120
Hpy8I GTNNAC 1 cut(s) 688
HpyAV CCTTC 3 cut(s) 329, 431, 515
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4IV ACGT 3 cut(s) 194, 216, 640
HpyCH4V TGCA 4 cut(s) 43, 472, 497, 751
HpyF3I CTNAG 2 cut(s) 110, 517
HpySE526I ACGT 3 cut(s) 194, 216, 640
Hsp92II CATG 3 cut(s) 595, 625, 755
HspAI GCGC 2 cut(s) 514, 596
Ksp22I TGATCA 3 cut(s) 363, 478, 790
Kzo9I GATC 4 cut(s) 101, 363, 478, 790
LguI GCTCTTC 1 cut(s) 314
Lsp1109I GCAGC 2 cut(s) 27, 484
LweI GCATC 1 cut(s) 96
MaeI CTAG 1 cut(s) 609
MaeII ACGT 3 cut(s) 194, 216, 640
MaeIII GTNAC 2 cut(s) 284, 433
MalI GATC 4 cut(s) 103, 365, 480, 792
MboI GATC 4 cut(s) 101, 363, 478, 790
MboII GAAGA 3 cut(s) 151, 331, 389
MfeI CAATTG 1 cut(s) 726
MluCI AATT 3 cut(s) 240, 353, 726
MmeI TCCRAC 3 cut(s) 150, 211, 737
MnlI CCTC 3 cut(s) 275, 694, 724
Mph1103I ATGCAT 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 678
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 1 cut(s) 586
MspR9I CCNGG 1 cut(s) 428
MunI CAATTG 1 cut(s) 726
MvaI CCWGG 1 cut(s) 428
NdeI CATATG 1 cut(s) 499
NdeII GATC 4 cut(s) 101, 363, 478, 790
NlaIII CATG 3 cut(s) 595, 625, 755
NmuCI GTSAC 1 cut(s) 433
NsiI ATGCAT 1 cut(s) 499
PasI CCCWGGG 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 314
PdmI GAANNNNTTC 1 cut(s) 678
PfeI GAWTC 3 cut(s) 116, 225, 776
PkrI GCNGC 2 cut(s) 42, 474
Psp1406I AACGTT 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 426
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 1 cut(s) 426
PspPI GGNCC 1 cut(s) 528
PstI CTGCAG 1 cut(s) 45
PstNI CAGNNNCTG 1 cut(s) 49
RsaI GTAC 4 cut(s) 464, 590, 722, 766
RsaNI GTAC 4 cut(s) 463, 589, 721, 765
RseI CAYNNNNRTG 1 cut(s) 371
SapI GCTCTTC 1 cut(s) 314
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 2 cut(s) 41, 473
Sau3AI GATC 4 cut(s) 101, 363, 478, 790
Sau96I GGNCC 1 cut(s) 528
ScaI AGTACT 1 cut(s) 722
ScrFI CCNGG 1 cut(s) 428
SfaNI GCATC 1 cut(s) 96
SfcI CTRYAG 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 371
Sse9I AATT 3 cut(s) 240, 353, 726
SsiI CCGC 1 cut(s) 207
SspI AATATT 1 cut(s) 342
SspMI CTAG 1 cut(s) 609
StyD4I CCNGG 1 cut(s) 426
TaaI ACNGT 1 cut(s) 439
TaiI ACGT 3 cut(s) 197, 219, 643
TaqI TCGA 1 cut(s) 358
TasI AATT 3 cut(s) 240, 353, 726
TatI WGTACW 3 cut(s) 462, 720, 764
TfiI GAWTC 3 cut(s) 116, 225, 776
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 2 cut(s) 40, 472
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 3 cut(s) 610, 651, 671
XagI CCTNNNNNAGG 1 cut(s) 425
XapI RAATTY 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 678
XspI CTAG 1 cut(s) 609
ZrmI AGTACT 1 cut(s) 722
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.