Rmu_sc0037686.1_g000001

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0037686.1
OG0014774
Physical Location & Seq
Forward (+)
1 .. 1043
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0037686.1_g000001.1.cds

Sequence Viewer

Length: 484 bp
gtacatactgcagcggggtggttctggtttctatgcatatggaatattcgagcgccttgatgggtggccagcggtacaggatatggatcaaattagaatggtttacaagcttgaacaagccaaatttcattatatggcgttatcagacaccaggcaaagaagcatgccaacagcagcagaccgtggtcgtggtcagaaacttgcgtataaagaagcttgtcttttgactaatccaagcaatcctgactttagaggagaggtagatgacaagtaccagtactcaatcgaggacaaagataacaaagttcatggttggattagtactgatgaatctgtgggattctggatgatcacacccagcgacgaatttcgtacaggaggcccctttaagcaggacctcacctcccatgttggtccaaccacactctccgtaagtaccgagtacaccggtctagctagtttctttcatcatgttcctttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

160

Amino Acids

18.23

Weight (kDa)

5.7

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 14, 72
AclWI GGATC 1 cut(s) 94
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 2 cut(s) 123, 366
AfaI GTAC 8 cut(s) 3, 76, 273, 279, 323, 374, 437, 444
AgeI ACCGGT 1 cut(s) 447
AgsI TTSAA 1 cut(s) 114
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 3 cut(s) 110, 216, 456
AluI AGCT 3 cut(s) 110, 216, 456
AlwI GGATC 1 cut(s) 94
AoxI GGCC 2 cut(s) 66, 380
ApeKI GCWGC 2 cut(s) 11, 174
ApoI RAATTY 2 cut(s) 123, 366
AsiGI ACCGGT 1 cut(s) 447
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 3 cut(s) 381, 395, 414
AsuHPI GGTGA 1 cut(s) 392
AvaII GGWCC 2 cut(s) 395, 414
BalI TGGCCA 1 cut(s) 68
BbvI GCAGC 2 cut(s) 23, 186
BccI CCATC 1 cut(s) 54
BciT130I CCWGG 1 cut(s) 152
BclI TGATCA 1 cut(s) 349
BfaI CTAG 2 cut(s) 453, 457
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 56
BisI GCNGC 2 cut(s) 12, 175
BlsI GCNGC 2 cut(s) 13, 176
BmcAI AGTACT 2 cut(s) 279, 323
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 2 cut(s) 395, 414
BmgT120I GGNCC 3 cut(s) 381, 395, 414
BmiI GGNNCC 1 cut(s) 383
BmrFI CCNGG 1 cut(s) 152
BoxI GACNNNNGTC 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 182
BsaWI WCCGGW 1 cut(s) 447
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bse118I RCCGGY 1 cut(s) 447
Bse1I ACTGG 1 cut(s) 275
Bse8I GATNNNNATC 1 cut(s) 85
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 1 cut(s) 182
BseGI GGATG 1 cut(s) 352
BseJI GATNNNNATC 1 cut(s) 85
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 1 cut(s) 268
BseXI GCAGC 2 cut(s) 23, 186
BseYI CCCAGC 1 cut(s) 357
BshFI GGCC 2 cut(s) 68, 382
BshTI ACCGGT 1 cut(s) 447
BsiSI CCGG 1 cut(s) 448
BsnI GGCC 2 cut(s) 68, 382
Bsp143I GATC 2 cut(s) 86, 349
BspACI CCGC 2 cut(s) 14, 72
BspANI GGCC 2 cut(s) 68, 382
BspLI GGNNCC 1 cut(s) 383
BspMAI CTGCAG 1 cut(s) 13
BspPI GGATC 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 447
BsrI ACTGG 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 447
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 2 cut(s) 86, 349
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 70, 165
BstDSI CCRYGG 1 cut(s) 182
BstF5I GGATG 1 cut(s) 352
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 2 cut(s) 89, 352
BstMBI GATC 2 cut(s) 86, 349
BstNI CCWGG 1 cut(s) 152
BstNSI RCATGY 1 cut(s) 167
BstPAI GACNNNNGTC 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 150
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 23, 186
BsuRI GGCC 2 cut(s) 68, 382
BtgI CCRYGG 1 cut(s) 182
BtsCI GGATG 1 cut(s) 352
Cac8I GCNNGC 2 cut(s) 70, 165
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 447
Cfr13I GGNCC 3 cut(s) 381, 395, 414
Csp6I GTAC 8 cut(s) 2, 75, 272, 278, 322, 373, 436, 443
CspAI ACCGGT 1 cut(s) 447
CviAII CATG 4 cut(s) 164, 309, 408, 471
CviJI RGCY 6 cut(s) 68, 110, 120, 216, 382, 456
CviKI_1 RGCY 6 cut(s) 68, 110, 120, 216, 382, 456
CviQI GTAC 8 cut(s) 2, 75, 272, 278, 322, 373, 436, 443
DpnI GATC 2 cut(s) 88, 351
DpnII GATC 2 cut(s) 86, 349
EaeI YGGCCR 1 cut(s) 66
Eco47I GGWCC 2 cut(s) 395, 414
EcoO109I RGGNCCY 2 cut(s) 381, 395
EcoRII CCWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 38
FaeI CATG 4 cut(s) 167, 312, 411, 474
FalI AAGNNNNNCTT 2 cut(s) 205, 237
FatI CATG 4 cut(s) 163, 308, 407, 470
FauI CCCGC 1 cut(s) 7
FauNDI CATATG 1 cut(s) 38
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 2 cut(s) 12, 175
FokI GGATG 1 cut(s) 359
Fsp4HI GCNGC 2 cut(s) 12, 175
FspBI CTAG 2 cut(s) 453, 457
GlaI GCGC 1 cut(s) 54
GluI GCNGC 2 cut(s) 12, 175
GsaI CCCAGC 1 cut(s) 361
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 68, 382
HapII CCGG 1 cut(s) 448
HhaI GCGC 1 cut(s) 55
Hin1II CATG 4 cut(s) 167, 312, 411, 474
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HindIII AAGCTT 2 cut(s) 108, 214
HinfI GANTC 2 cut(s) 330, 340
HpaII CCGG 1 cut(s) 448
HphI GGTGA 1 cut(s) 392
Hpy166II GTNNAC 2 cut(s) 104, 445
Hpy188I TCNGA 2 cut(s) 146, 196
Hpy188III TCNNGA 2 cut(s) 243, 344
Hpy8I GTNNAC 2 cut(s) 104, 445
Hpy99I CGWCG 1 cut(s) 366
HpyCH4III ACNGT 1 cut(s) 183
HpyCH4V TGCA 2 cut(s) 11, 36
Hsp92II CATG 4 cut(s) 167, 312, 411, 474
HspAI GCGC 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 2 cut(s) 86, 349
Lsp1109I GCAGC 2 cut(s) 23, 186
MaeI CTAG 2 cut(s) 453, 457
MalI GATC 2 cut(s) 88, 351
MboI GATC 2 cut(s) 86, 349
MlsI TGGCCA 1 cut(s) 68
MluCI AATT 3 cut(s) 91, 123, 366
MluNI TGGCCA 1 cut(s) 68
MmeI TCCRAC 2 cut(s) 294, 441
MnlI CCTC 6 cut(s) 246, 251, 281, 372, 408, 413
Mox20I TGGCCA 1 cut(s) 68
Mph1103I ATGCAT 1 cut(s) 38
MscI TGGCCA 1 cut(s) 68
MseI TTAA 1 cut(s) 388
Msp20I TGGCCA 1 cut(s) 68
MspA1I CMGCKG 2 cut(s) 14, 72
MspI CCGG 1 cut(s) 448
MspR9I CCNGG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 152
NdeI CATATG 1 cut(s) 38
NdeII GATC 2 cut(s) 86, 349
NlaIII CATG 4 cut(s) 167, 312, 411, 474
NlaIV GGNNCC 1 cut(s) 383
NsiI ATGCAT 1 cut(s) 38
NspI RCATGY 1 cut(s) 167
PaeI GCATGC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 330, 340
PinAI ACCGGT 1 cut(s) 447
PkrI GCNGC 2 cut(s) 13, 176
PpuMI RGGWCCY 1 cut(s) 395
PshAI GACNNNNGTC 1 cut(s) 184
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 1 cut(s) 150
PspFI CCCAGC 1 cut(s) 357
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 383
PspPI GGNCC 3 cut(s) 381, 395, 414
PspPPI RGGWCCY 1 cut(s) 395
PstI CTGCAG 1 cut(s) 13
RsaI GTAC 8 cut(s) 3, 76, 273, 279, 323, 374, 437, 444
RsaNI GTAC 8 cut(s) 2, 75, 272, 278, 322, 373, 436, 443
SaqAI TTAA 1 cut(s) 388
SatI GCNGC 2 cut(s) 12, 175
Sau3AI GATC 2 cut(s) 86, 349
Sau96I GGNCC 3 cut(s) 381, 395, 414
ScaI AGTACT 2 cut(s) 279, 323
ScrFI CCNGG 1 cut(s) 152
SetI ASST 6 cut(s) 112, 218, 262, 400, 405, 458
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 2 cut(s) 395, 414
SphI GCATGC 1 cut(s) 167
Sse9I AATT 3 cut(s) 91, 123, 366
SsiI CCGC 2 cut(s) 14, 72
SspI AATATT 1 cut(s) 46
SspMI CTAG 2 cut(s) 453, 457
StyD4I CCNGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 183
TaqI TCGA 2 cut(s) 49, 286
TasI AATT 3 cut(s) 91, 123, 366
TatI WGTACW 3 cut(s) 277, 321, 442
TfiI GAWTC 2 cut(s) 330, 340
Tru1I TTAA 1 cut(s) 388
Tru9I TTAA 1 cut(s) 388
TseI GCWGC 2 cut(s) 11, 174
TspDTI ATGAA 4 cut(s) 117, 297, 343, 456
TspGWI ACGGA 1 cut(s) 419
VpaK11BI GGWCC 2 cut(s) 395, 414
XapI RAATTY 2 cut(s) 123, 366
XceI RCATGY 1 cut(s) 167
XspI CTAG 2 cut(s) 453, 457
ZrmI AGTACT 2 cut(s) 279, 323
Zsp2I ATGCAT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.