Rh2CG039200

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0014774
Physical Location & Seq
Forward (+)
3119549 .. 3120330
782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG039200.1

Sequence Viewer

Length: 483 bp
ATGGGGTTGTATCGTGACACTTTTCTAAACGTGTATGGAGGAATTGACAACTTACTTGCAATTAACAATGAGGAAGGTGATAGAGGGTACTGGGATGTGGTTTGGAGTAACCCGGGAGAGACGAAAAACACACAATATAGACTACATGGAACACAATTTAAAGTTATCACGGCGAGGCCAGACCAAGTAGAAGTTTCTTTCATCACCACATACAATGCCAGCCTCAACAGGGGCGTGACAGTTCCGGTGAATGTTGACAAAAGGTACATAAGGCAGCGAGGACGTTCCGGGTTCTATACATATGCCATCCTTGAGCGTCTTGAAGGCTGGCCGGGAGGGGACATGCAGACACTTAGAGTGGCTTACAAGCTTCATCAAGCCACGTTTCGATATATGGCGCTATCGGACACTAGGCAAAGGTTCATGCCAACAGCCGATGACCGAGCACGCGGTAGGCCACATTCAAAGAAGTTGTTCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

160

Amino Acids

18.6

Weight (kDa)

9.73

Isoelectric Point (pI)

20.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 12 - 153 3.4e-55 Rhamnogalacturonate lyase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 450
AciI CCGC 1 cut(s) 450
AcoI YGGCCR 1 cut(s) 329
AfaI GTAC 2 cut(s) 89, 266
AfiI CCNNNNNNNGG 1 cut(s) 229
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 2 cut(s) 323, 465
AluBI AGCT 1 cut(s) 370
AluI AGCT 1 cut(s) 370
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 1 cut(s) 113
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 3 cut(s) 176, 329, 455
ApeKI GCWGC 1 cut(s) 274
Asp700I GAANNNNTTC 1 cut(s) 473
AspLEI GCGC 1 cut(s) 400
AsuC2I CCSGG 4 cut(s) 113, 114, 289, 333
AsuHPI GGTGA 3 cut(s) 89, 196, 259
AvaI CYCGRG 1 cut(s) 112
BarI GAAGNNNNNNTAC 1 cut(s) 461
Bbv12I GWGCWC 1 cut(s) 448
BbvI GCAGC 1 cut(s) 286
BccI CCATC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 186
BcnI CCSGG 4 cut(s) 113, 114, 289, 333
BcoDI GTCTC 1 cut(s) 113
BfaI CTAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 401
BisI GCNGC 1 cut(s) 275
BlsI GCNGC 1 cut(s) 276
Bme1390I CCNGG 4 cut(s) 113, 114, 289, 333
BmeT110I CYCGRG 1 cut(s) 112
BmrFI CCNGG 4 cut(s) 113, 114, 289, 333
BmrI ACTGGG 1 cut(s) 100
BmuI ACTGGG 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 4 cut(s) 113, 114, 289, 333
BsaJI CCNNGG 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse1I ACTGG 1 cut(s) 95
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 2 cut(s) 100, 306
BseLI CCNNNNNNNGG 1 cut(s) 229
BseNI ACTGG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 286
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 3 cut(s) 178, 331, 457
BsiHKAI GWGCWC 1 cut(s) 448
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 4 cut(s) 113, 245, 288, 332
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 1 cut(s) 113
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 353
BsnI GGCC 3 cut(s) 178, 331, 457
BsoBI CYCGRG 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 448
BspACI CCGC 1 cut(s) 450
BspANI GGCC 3 cut(s) 178, 331, 457
BspFNI CGCG 1 cut(s) 450
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 112
Bst4CI ACNGT 1 cut(s) 241
BstC8I GCNNGC 3 cut(s) 220, 329, 448
BstDEI CTNAG 1 cut(s) 353
BstF5I GGATG 2 cut(s) 100, 306
BstFNI CGCG 1 cut(s) 450
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 1 cut(s) 400
BstMAI GTCTC 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 4 cut(s) 111, 112, 287, 331
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 1 cut(s) 286
BsuRI GGCC 3 cut(s) 178, 331, 457
BtsCI GGATG 2 cut(s) 100, 306
Cac8I GCNNGC 3 cut(s) 220, 329, 448
CfoI GCGC 1 cut(s) 400
Cfr9I CCCGGG 1 cut(s) 112
CseI GACGC 1 cut(s) 305
Csp6I GTAC 2 cut(s) 88, 265
CviAII CATG 3 cut(s) 146, 343, 424
CviJI RGCY 9 cut(s) 178, 222, 327, 331, 362, 370, 380, 434, 457
CviKI_1 RGCY 9 cut(s) 178, 222, 327, 331, 362, 370, 380, 434, 457
CviQI GTAC 2 cut(s) 88, 265
DdeI CTNAG 1 cut(s) 353
DraI TTTAAA 1 cut(s) 160
EaeI YGGCCR 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 112
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 3 cut(s) 149, 346, 427
FaqI GGGAC 1 cut(s) 353
FatI CATG 3 cut(s) 145, 342, 423
FauNDI CATATG 1 cut(s) 301
Fnu4HI GCNGC 1 cut(s) 275
FokI GGATG 2 cut(s) 107, 293
Fsp4HI GCNGC 1 cut(s) 275
FspBI CTAG 1 cut(s) 411
GlaI GCGC 1 cut(s) 399
GluI GCNGC 1 cut(s) 275
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 3 cut(s) 178, 331, 457
HapII CCGG 4 cut(s) 113, 245, 288, 332
HgaI GACGC 1 cut(s) 305
HhaI GCGC 1 cut(s) 400
Hin1II CATG 3 cut(s) 149, 346, 427
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 368
HpaII CCGG 4 cut(s) 113, 245, 288, 332
HphI GGTGA 3 cut(s) 89, 196, 259
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 1 cut(s) 406
Hpy188III TCNNGA 2 cut(s) 14, 320
Hpy8I GTNNAC 1 cut(s) 256
HpyAV CCTTC 2 cut(s) 68, 317
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4IV ACGT 3 cut(s) 30, 283, 383
HpyCH4V TGCA 2 cut(s) 59, 346
HpyF3I CTNAG 1 cut(s) 353
HpySE526I ACGT 3 cut(s) 30, 283, 383
Hsp92II CATG 3 cut(s) 149, 346, 427
HspAI GCGC 1 cut(s) 398
LpnPI CCDG 9 cut(s) 76, 126, 192, 214, 232, 258, 301, 313, 345
Lsp1109I GCAGC 1 cut(s) 286
MaeI CTAG 1 cut(s) 411
MaeII ACGT 3 cut(s) 30, 283, 383
MaeIII GTNAC 3 cut(s) 14, 107, 235
MhlI GDGCHC 1 cut(s) 448
MluCI AATT 3 cut(s) 42, 60, 155
MnlI CCTC 7 cut(s) 32, 64, 77, 168, 233, 272, 329
MroXI GAANNNNTTC 1 cut(s) 473
MseI TTAA 2 cut(s) 63, 159
MspI CCGG 4 cut(s) 113, 245, 288, 332
MspR9I CCNGG 4 cut(s) 113, 114, 289, 333
MvnI CGCG 1 cut(s) 450
NciI CCSGG 4 cut(s) 113, 114, 289, 333
NdeI CATATG 1 cut(s) 301
NlaIII CATG 3 cut(s) 149, 346, 427
NmuCI GTSAC 2 cut(s) 14, 235
NspI RCATGY 1 cut(s) 346
PdmI GAANNNNTTC 1 cut(s) 473
PkrI GCNGC 1 cut(s) 276
RsaI GTAC 2 cut(s) 89, 266
RsaNI GTAC 2 cut(s) 88, 265
SaqAI TTAA 2 cut(s) 63, 159
SatI GCNGC 1 cut(s) 275
ScrFI CCNGG 4 cut(s) 113, 114, 289, 333
SduI GDGCHC 1 cut(s) 448
SetI ASST 7 cut(s) 33, 79, 266, 286, 372, 386, 422
SmaI CCCGGG 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 3 cut(s) 42, 60, 155
SsiI CCGC 1 cut(s) 450
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 4 cut(s) 111, 112, 287, 331
TaaI ACNGT 1 cut(s) 241
TaiI ACGT 3 cut(s) 33, 286, 386
TaqI TCGA 1 cut(s) 388
TaqII GACCGA 1 cut(s) 456
TasI AATT 3 cut(s) 42, 60, 155
Tru1I TTAA 2 cut(s) 63, 159
Tru9I TTAA 2 cut(s) 63, 159
TseFI GTSAC 2 cut(s) 14, 235
TseI GCWGC 1 cut(s) 274
Tsp45I GTSAC 2 cut(s) 14, 235
TspDTI ATGAA 3 cut(s) 190, 362, 412
TspMI CCCGGG 1 cut(s) 112
XceI RCATGY 1 cut(s) 346
XmaI CCCGGG 1 cut(s) 112
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.