RLG00000020538

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014530
Physical Location & Seq
Forward (+)
66077599 .. 66079224
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020538

Sequence Viewer

Length: 1452 bp
ATGGCTACTGAAATGAGGTACACTCCACTCTGTTTGTTCTGTATATTAGCTGCAACATTACTCCTCATCATCATTTTCTTGAAGCACAAGAAACTCTTTGGCACAAGCAAGAGAAAACTACCACCTGGGGAAATGGGGCTTCCCTGGATAGGCCAAACAATGGAGTTCTACAGAGCTCAACAGAAAAACAGTTTGTTCGATGACTTCGTTCAGCCCCGGATTGCAAAATATGGCAAAATCTTCACAACACATCTAATGGGATCACCAACTGTGATTGTGAATGGAGCCAATGCTAACAGATTCCTGTTGTCCAATGAGTTCAAGCTGGTGATAAGTTCGTGGCCTTCAACATCGGTTCAGCTTATGGGGAAGGACTCCATCATGGAAAAGCAAGGGGAGAGGCACCGCTGCCTTCGCGGGCTTATAGGCTCAAGCCTTGGCCAGGCAGGGCTTGAGACTTTAGTGCCTAAAGTATGTAAATTAATGAAGTTGCACTTGGACACAAAATGGAAAGGCCAGGATGAGATCAGCCTGTATCGTGCAACGAAAGTCTTGACTTTTTCGATAGTGTTTGAGTGCTTGTTGGGGATCAATGTGAAGGCAGATATGTTGGGAACGTTTGAGAGGGTTTTGGAAGGGGTTTTTGCAGCACCAATTAATTTTCCGGGGTCCAGGTTTTGGCGAGCAAAGAAAGCGAGGAAGGAGAATGAGAAAATACTGGTTAAAGTAGTAAGAGAGAAGAAGAAGCACATGGAAGAAAGCAAGAAAGAAGAAGAAGAGCAGGGAGGGATGTTGCTGTCTCGATTAGTGGCTGGGATGATTCGAGGAGAAATTACCGAAGAGGAGGTTGTTGATAATGTGGTGTTGCTGGTGTTTGCAGCTCATGATACTACTTCTTTTGCCATTGCCATGACATTCAAAATGTTGGCTCAGCATCCATATTGCCACAGTGTCCTGCTTAAAGAACATGCGGAGATAATGAGAAGCAAAAAAACTGGTGATCAAAATCTGACATTGGATGATACGAAGAACATGAAGTACACCTGGCAGGTTGCTCGAGAAAGCATGCGTCTCTTCCCTCCTATCTTCGGCTCTTTCAGGAAAGCCATTGTTGATATTGAATATGAAGGATTCACTATTCCACAAGGATGGAAGGTGTTGTGGACAACATATGGAACACATTACAATTCAGAATATTTTGAAGATCCTTCGAGTTTCAATCCAAGCCGGTTTGAGGAGCATGTTCCACCATATGCCTTTCTGGCATTCGGAGGAGGGCCAAGAGTTTGTGCAGGGTACCAACTTGCTAAGCTGAACATCCTCATTTTCGTGCATTTCATTGTGACTCGTTATGACTGGTCTTTGCTATATCCTGATGAGCCTATATCCATGGATCCTCTTCCATTTCCTTCATATGGAATGCCTATTAAAATTACTCCAAAGTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

484

Amino Acids

55.17

Weight (kDa)

9.16

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 41 - 467 7.6e-60 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014530)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42850 AT2G42850
fragaria_vesca FvH4_6g38110
malus_domestica MD17G1140500.v1.1
prunus_persica Prupe.3G171100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0151171
rosa_laevigata RLG00000020538
rosa_multiflora Rmu_sc0002298.1_g000017 Rmu_sc0002516.1_g000001
rosa_roxburghii Rroxscaffold_2G00096680
rosa_rugosa Rorug02G0425400
rosa_samantha Rh2BG497900 Rh2CG471800 Rh2CG472300 Rh2DG508800
rosa_wichuraiana Rw2G039800 Rw2G040210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1039
Acc65I GGTACC 1 cut(s) 1296
AccB1I GGYRCC 2 cut(s) 402, 1296
AccB7I CCANNNNNTGG 2 cut(s) 160, 678
AccII CGCG 1 cut(s) 417
AciI CCGC 3 cut(s) 406, 417, 971
AclI AACGTT 1 cut(s) 617
AclWI GGATC 5 cut(s) 268, 596, 1199, 1388, 1401
AcoI YGGCCR 1 cut(s) 439
AfaI GTAC 3 cut(s) 20, 1040, 1298
AfiI CCNNNNNNNGG 7 cut(s) 149, 160, 442, 678, 1088, 1234, 1415
AgsI TTSAA 7 cut(s) 82, 322, 348, 919, 1121, 1202, 1219
AjnI CCWGG 6 cut(s) 124, 143, 441, 516, 671, 1043
AjuI GAANNNNNNNTTGG 2 cut(s) 479, 511
AluBI AGCT 6 cut(s) 50, 176, 325, 361, 881, 1312
AluI AGCT 6 cut(s) 50, 176, 325, 361, 881, 1312
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 3 cut(s) 449, 804, 1076
AlwI GGATC 5 cut(s) 268, 596, 1199, 1388, 1401
Ama87I CYCGRG 1 cut(s) 1056
AoxI GGCC 5 cut(s) 151, 341, 439, 514, 1277
ApeKI GCWGC 4 cut(s) 50, 408, 647, 878
AseI ATTAAT 2 cut(s) 482, 657
Asp718I GGTACC 1 cut(s) 1296
AspS9I GGNCC 2 cut(s) 669, 1277
AsuC2I CCSGG 2 cut(s) 217, 666
AsuHPI GGTGA 3 cut(s) 255, 340, 1010
AvaI CYCGRG 1 cut(s) 1056
AvaII GGWCC 1 cut(s) 669
BalI TGGCCA 1 cut(s) 441
BamHI GGATCC 1 cut(s) 1393
BanI GGYRCC 2 cut(s) 402, 1296
BanII GRGCYC 1 cut(s) 178
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 4 cut(s) 37, 395, 659, 890
BccI CCATC 2 cut(s) 386, 1143
BcgI CGANNNNNNTGC 2 cut(s) 1037, 1071
BciT130I CCWGG 6 cut(s) 126, 145, 443, 518, 673, 1045
BclI TGATCA 1 cut(s) 1000
BcnI CCSGG 2 cut(s) 217, 666
BcoDI GTCTC 3 cut(s) 449, 804, 1076
BfmI CTRYAG 1 cut(s) 169
BfuAI ACCTGC 1 cut(s) 1039
BglI GCCNNNNNGGC 1 cut(s) 1262
BisI GCNGC 4 cut(s) 51, 409, 648, 879
BlpI GCTNAGC 2 cut(s) 930, 1308
BlsI GCNGC 4 cut(s) 52, 410, 649, 880
Bme1390I CCNGG 8 cut(s) 126, 145, 217, 443, 518, 666, 673, 1045
Bme18I GGWCC 1 cut(s) 669
BmeT110I CYCGRG 1 cut(s) 1056
BmgT120I GGNCC 2 cut(s) 669, 1277
BmiI GGNNCC 5 cut(s) 286, 404, 670, 1298, 1395
BmrFI CCNGG 8 cut(s) 126, 145, 217, 443, 518, 666, 673, 1045
BmsI GCATC 1 cut(s) 943
BplI GAGNNNNNCTC 2 cut(s) 7, 39
Bpu1102I GCTNAGC 2 cut(s) 930, 1308
BpuEI CTTGAG 2 cut(s) 415, 473
BpuMI CCSGG 2 cut(s) 217, 666
BsaBI GATNNNNATC 1 cut(s) 1005
BsaJI CCNNGG 6 cut(s) 125, 143, 215, 436, 665, 1389
Bsc4I CCNNNNNNNGG 7 cut(s) 149, 160, 442, 678, 1088, 1234, 1415
Bse118I RCCGGY 1 cut(s) 1227
Bse1I ACTGG 3 cut(s) 723, 1000, 1361
Bse3DI GCAATG 1 cut(s) 903
Bse8I GATNNNNATC 1 cut(s) 1005
BseBI CCWGG 6 cut(s) 126, 145, 443, 518, 673, 1045
BseDI CCNNGG 6 cut(s) 125, 143, 215, 436, 665, 1389
BseGI GGATG 7 cut(s) 526, 795, 822, 934, 1024, 1154, 1317
BseJI GATNNNNATC 1 cut(s) 1005
BseLI CCNNNNNNNGG 7 cut(s) 149, 160, 442, 678, 1088, 1234, 1415
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 1 cut(s) 944
BseNI ACTGG 3 cut(s) 723, 1000, 1361
BseRI GAGGAG 5 cut(s) 53, 840, 857, 1250, 1287
BseXI GCAGC 4 cut(s) 37, 395, 659, 890
BseYI CCCAGC 1 cut(s) 812
BsgI GTGCAG 1 cut(s) 1311
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 5 cut(s) 153, 343, 441, 516, 1279
BshNI GGYRCC 2 cut(s) 402, 1296
BsiHKAI GWGCWC 1 cut(s) 178
BsiHKCI CYCGRG 1 cut(s) 1056
BsiSI CCGG 3 cut(s) 217, 665, 1228
BslI CCNNNNNNNGG 7 cut(s) 149, 160, 442, 678, 1088, 1234, 1415
BsmAI GTCTC 3 cut(s) 449, 804, 1076
BsmBI CGTCTC 1 cut(s) 1076
BsmI GAATGC 2 cut(s) 1265, 1425
BsnI GGCC 5 cut(s) 153, 343, 441, 516, 1279
BsoBI CYCGRG 1 cut(s) 1056
Bsp1286I GDGCHC 1 cut(s) 178
Bsp143I GATC 6 cut(s) 260, 525, 588, 1000, 1204, 1393
Bsp1720I GCTNAGC 2 cut(s) 930, 1308
Bsp19I CCATGG 1 cut(s) 1389
BspACI CCGC 3 cut(s) 406, 417, 971
BspANI GGCC 5 cut(s) 153, 343, 441, 516, 1279
BspCNI CTCAG 1 cut(s) 943
BspFNI CGCG 1 cut(s) 417
BspHI TCATGA 1 cut(s) 883
BspLI GGNNCC 5 cut(s) 286, 404, 670, 1298, 1395
BspMI ACCTGC 1 cut(s) 1039
BspPI GGATC 5 cut(s) 268, 596, 1199, 1388, 1401
BspQI GCTCTTC 1 cut(s) 771
BspT107I GGYRCC 2 cut(s) 402, 1296
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 1227
BsrI ACTGG 3 cut(s) 723, 1000, 1361
BssAI RCCGGY 1 cut(s) 1227
BssECI CCNNGG 6 cut(s) 125, 143, 215, 436, 665, 1389
BssMI GATC 6 cut(s) 260, 525, 588, 1000, 1204, 1393
BssT1I CCWWGG 2 cut(s) 436, 1389
Bst2UI CCWGG 6 cut(s) 126, 145, 443, 518, 673, 1045
Bst4CI ACNGT 3 cut(s) 191, 271, 950
Bst6I CTCTTC 4 cut(s) 771, 834, 1079, 1404
BstC8I GCNNGC 3 cut(s) 419, 684, 1067
BstDEI CTNAG 2 cut(s) 930, 1308
BstDSI CCRYGG 1 cut(s) 1389
BstENI CCTNNNNNAGG 1 cut(s) 440
BstF5I GGATG 7 cut(s) 526, 795, 822, 934, 1024, 1154, 1317
BstFNI CGCG 1 cut(s) 417
BstKTI GATC 6 cut(s) 263, 528, 591, 1003, 1207, 1396
BstMAI GTCTC 3 cut(s) 449, 804, 1076
BstMBI GATC 6 cut(s) 260, 525, 588, 1000, 1204, 1393
BstMWI GCNNNNNNNGC 3 cut(s) 414, 692, 1262
BstNI CCWGG 6 cut(s) 126, 145, 443, 518, 673, 1045
BstNSI RCATGY 3 cut(s) 971, 1069, 1244
BstSCI CCNGG 8 cut(s) 124, 143, 215, 441, 516, 664, 671, 1043
BstSFI CTRYAG 1 cut(s) 169
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 4 cut(s) 37, 395, 659, 890
BstX2I RGATCY 2 cut(s) 1204, 1393
BstXI CCANNNNNNTGG 1 cut(s) 1149
BstYI RGATCY 2 cut(s) 1204, 1393
BsuRI GGCC 5 cut(s) 153, 343, 441, 516, 1279
BtgI CCRYGG 1 cut(s) 1389
BtsCI GGATG 7 cut(s) 526, 795, 822, 934, 1024, 1154, 1317
BtsIMutI CAGTG 1 cut(s) 955
BveI ACCTGC 1 cut(s) 1039
Cac8I GCNNGC 3 cut(s) 419, 684, 1067
CciI TCATGA 1 cut(s) 883
Cfr10I RCCGGY 1 cut(s) 1227
Cfr13I GGNCC 2 cut(s) 669, 1277
CseI GACGC 1 cut(s) 1058
Csp6I GTAC 3 cut(s) 19, 1039, 1297
CspCI CAANNNNNGTGG 2 cut(s) 15, 50
CviAII CATG 9 cut(s) 382, 751, 884, 910, 968, 1033, 1066, 1241, 1390
CviQI GTAC 3 cut(s) 19, 1039, 1297
DdeI CTNAG 2 cut(s) 930, 1308
DpnI GATC 6 cut(s) 262, 527, 590, 1002, 1206, 1395
DpnII GATC 6 cut(s) 260, 525, 588, 1000, 1204, 1393
EaeI YGGCCR 1 cut(s) 439
Eam1104I CTCTTC 4 cut(s) 771, 834, 1079, 1404
EarI CTCTTC 4 cut(s) 771, 834, 1079, 1404
Ecl136II GAGCTC 1 cut(s) 176
Eco130I CCWWGG 2 cut(s) 436, 1389
Eco24I GRGCYC 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 669
Eco53kI GAGCTC 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 1056
EcoICRI GAGCTC 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 440
EcoRII CCWGG 6 cut(s) 124, 143, 441, 516, 671, 1043
EcoT14I CCWWGG 2 cut(s) 436, 1389
EcoT38I GRGCYC 1 cut(s) 178
ErhI CCWWGG 2 cut(s) 436, 1389
Esp3I CGTCTC 1 cut(s) 1076
FaeI CATG 9 cut(s) 385, 754, 887, 913, 971, 1036, 1069, 1244, 1393
FalI AAGNNNNNCTT 4 cut(s) 80, 112, 479, 511
FatI CATG 9 cut(s) 381, 750, 883, 909, 967, 1032, 1065, 1240, 1389
FauI CCCGC 1 cut(s) 410
FauNDI CATATG 3 cut(s) 1171, 1252, 1414
FbaI TGATCA 1 cut(s) 1000
Fnu4HI GCNGC 4 cut(s) 51, 409, 648, 879
FokI GGATG 7 cut(s) 533, 802, 829, 921, 1031, 1161, 1304
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 4 cut(s) 51, 409, 648, 879
GluI GCNGC 4 cut(s) 51, 409, 648, 879
GsaI CCCAGC 1 cut(s) 816
HaeIII GGCC 5 cut(s) 153, 343, 441, 516, 1279
HapII CCGG 3 cut(s) 217, 665, 1228
HgaI GACGC 1 cut(s) 1058
Hin1II CATG 9 cut(s) 385, 754, 887, 913, 971, 1036, 1069, 1244, 1393
HinfI GANTC 5 cut(s) 300, 374, 820, 1131, 1345
HpaII CCGG 3 cut(s) 217, 665, 1228
HphI GGTGA 3 cut(s) 255, 340, 1010
Hpy166II GTNNAC 3 cut(s) 21, 1041, 1164
Hpy188I TCNGA 3 cut(s) 1011, 1192, 1271
Hpy188III TCNNGA 7 cut(s) 79, 553, 801, 884, 1058, 1099, 1373
Hpy8I GTNNAC 3 cut(s) 21, 1041, 1164
HpyCH4III ACNGT 3 cut(s) 191, 271, 950
HpyCH4IV ACGT 1 cut(s) 617
HpyCH4V TGCA 8 cut(s) 53, 224, 493, 542, 647, 878, 1292, 1333
HpyF10VI GCNNNNNNNGC 3 cut(s) 414, 692, 1262
HpyF3I CTNAG 2 cut(s) 930, 1308
HpySE526I ACGT 1 cut(s) 617
Hsp92II CATG 9 cut(s) 385, 754, 887, 913, 971, 1036, 1069, 1244, 1393
KpnI GGTACC 1 cut(s) 1300
Ksp22I TGATCA 1 cut(s) 1000
Kzo9I GATC 6 cut(s) 260, 525, 588, 1000, 1204, 1393
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 2 cut(s) 284, 1237
Lsp1109I GCAGC 4 cut(s) 37, 395, 659, 890
LweI GCATC 1 cut(s) 943
MaeII ACGT 1 cut(s) 617
MaeIII GTNAC 1 cut(s) 1342
MalI GATC 6 cut(s) 262, 527, 590, 1002, 1206, 1395
MboI GATC 6 cut(s) 260, 525, 588, 1000, 1204, 1393
MflI RGATCY 2 cut(s) 1204, 1393
MhlI GDGCHC 1 cut(s) 178
MlsI TGGCCA 1 cut(s) 441
MluCI AATT 6 cut(s) 479, 654, 658, 831, 1186, 1431
MluNI TGGCCA 1 cut(s) 441
MlyI GAGTC 2 cut(s) 368, 1339
Mox20I TGGCCA 1 cut(s) 441
MscI TGGCCA 1 cut(s) 441
MseI TTAA 5 cut(s) 482, 657, 723, 960, 1428
MslI CAYNNNNRTG 3 cut(s) 908, 1147, 1328
Msp20I TGGCCA 1 cut(s) 441
MspA1I CMGCKG 1 cut(s) 408
MspI CCGG 3 cut(s) 217, 665, 1228
MspR9I CCNGG 8 cut(s) 126, 145, 217, 443, 518, 666, 673, 1045
Mva1269I GAATGC 2 cut(s) 1265, 1425
MvaI CCWGG 6 cut(s) 126, 145, 443, 518, 673, 1045
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 3 cut(s) 414, 692, 1262
NciI CCSGG 2 cut(s) 217, 666
NcoI CCATGG 1 cut(s) 1389
NdeI CATATG 3 cut(s) 1171, 1252, 1414
NdeII GATC 6 cut(s) 260, 525, 588, 1000, 1204, 1393
NlaIII CATG 9 cut(s) 385, 754, 887, 913, 971, 1036, 1069, 1244, 1393
NlaIV GGNNCC 5 cut(s) 286, 404, 670, 1298, 1395
NmuCI GTSAC 1 cut(s) 1342
NspI RCATGY 3 cut(s) 971, 1069, 1244
PaeI GCATGC 1 cut(s) 1069
PaeR7I CTCGAG 1 cut(s) 1056
PagI TCATGA 1 cut(s) 883
PciSI GCTCTTC 1 cut(s) 771
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 2 cut(s) 1265, 1425
PfeI GAWTC 3 cut(s) 300, 820, 1131
PflMI CCANNNNNTGG 2 cut(s) 160, 678
PkrI GCNGC 4 cut(s) 52, 410, 649, 880
PleI GAGTC 2 cut(s) 368, 1339
PpsI GAGTC 2 cut(s) 368, 1339
PshBI ATTAAT 2 cut(s) 482, 657
Psp124BI GAGCTC 1 cut(s) 178
Psp1406I AACGTT 1 cut(s) 617
Psp6I CCWGG 6 cut(s) 124, 143, 441, 516, 671, 1043
PspFI CCCAGC 1 cut(s) 812
PspGI CCWGG 6 cut(s) 124, 143, 441, 516, 671, 1043
PspN4I GGNNCC 5 cut(s) 286, 404, 670, 1298, 1395
PspPI GGNCC 2 cut(s) 669, 1277
PsrI GAACNNNNNNTAC 2 cut(s) 1022, 1054
PsuI RGATCY 2 cut(s) 1204, 1393
RsaI GTAC 3 cut(s) 20, 1040, 1298
RsaNI GTAC 3 cut(s) 19, 1039, 1297
RseI CAYNNNNRTG 3 cut(s) 908, 1147, 1328
SacI GAGCTC 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 771
SaqAI TTAA 5 cut(s) 482, 657, 723, 960, 1428
SatI GCNGC 4 cut(s) 51, 409, 648, 879
Sau3AI GATC 6 cut(s) 260, 525, 588, 1000, 1204, 1393
Sau96I GGNCC 2 cut(s) 669, 1277
SchI GAGTC 2 cut(s) 368, 1339
ScrFI CCNGG 8 cut(s) 126, 145, 217, 443, 518, 666, 673, 1045
SduI GDGCHC 1 cut(s) 178
SfaNI GCATC 1 cut(s) 943
SfcI CTRYAG 1 cut(s) 169
Sfr274I CTCGAG 1 cut(s) 1056
SinI GGWCC 1 cut(s) 669
SlaI CTCGAG 1 cut(s) 1056
SmiMI CAYNNNNRTG 3 cut(s) 908, 1147, 1328
SmlI CTYRAG 3 cut(s) 430, 452, 1056
SmoI CTYRAG 3 cut(s) 430, 452, 1056
SphI GCATGC 1 cut(s) 1069
Sse9I AATT 6 cut(s) 479, 654, 658, 831, 1186, 1431
SsiI CCGC 3 cut(s) 406, 417, 971
SspI AATATT 1 cut(s) 1196
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 8 cut(s) 124, 143, 215, 441, 516, 664, 671, 1043
StyI CCWWGG 2 cut(s) 436, 1389
TaaI ACNGT 3 cut(s) 191, 271, 950
TaiI ACGT 1 cut(s) 620
TaqI TCGA 6 cut(s) 198, 563, 802, 823, 1057, 1211
TasI AATT 6 cut(s) 479, 654, 658, 831, 1186, 1431
TatI WGTACW 1 cut(s) 1038
TfiI GAWTC 3 cut(s) 300, 820, 1131
Tru1I TTAA 5 cut(s) 482, 657, 723, 960, 1428
Tru9I TTAA 5 cut(s) 482, 657, 723, 960, 1428
TscAI CASTG 1 cut(s) 955
TseFI GTSAC 1 cut(s) 1342
TseI GCWGC 4 cut(s) 50, 408, 647, 878
Tsp45I GTSAC 1 cut(s) 1342
TspDTI ATGAA 5 cut(s) 500, 1049, 1140, 1327, 1401
TspRI CASTG 1 cut(s) 955
Van91I CCANNNNNTGG 2 cut(s) 160, 678
VpaK11BI GGWCC 1 cut(s) 669
VspI ATTAAT 2 cut(s) 482, 657
XagI CCTNNNNNAGG 1 cut(s) 440
XceI RCATGY 3 cut(s) 971, 1069, 1244
XhoI CTCGAG 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.