RLG00000023346

DTW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013032
Physical Location & Seq
Reverse (-)
22322885 .. 22323691
807 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023346

Sequence Viewer

Length: 807 bp
ATGGAAGCTGAAGACGACGGCTTCGAAACCGTCTACAACGCCACCTCTTCGCCGCCGCGGAGGCCGAGGTCCATCTGCAGCAACTGTAACCGGCCCAGCCCAGTCTGCCTCTGCAAGTCTCTCCCGGCCCAGCCCATCCCAACCCAAACGCAAATCATAATCCTTCACCATCCTCACGAGGCGCAGCACAAGCTCTCCACCACGCCGGTCCTGACAAAATGCCTGGCCAACTCCACCGCCATCGTCAGCCGCCGCCTCAAGCCGGGCCTGTCCCCGCTCCTCGACCAATCACCTTCCGCCATCTACCTCTTCCCGCCCTCGAAATCCTCACCGTCGGTCAGTTTATCCCGTCTCCGGATCCCCAGCCAGTCGCCGGTGGTGGTCGTGGCTTTCGACGCGACGTGGAAGCACGCGAAGGAGATGGTGAGGGCGAGCGAGGGGTTCCTGTCGAAATTCGCGAGGAGGGTGAGTTTGGATGTGGATGAGGATGTGAGGGGAGGGAGCATCTACGACTCGGAGTTGATTCTGAGGAAGGAGCCGTTTGGTGGGTGCGTGAGTACCATGGAGGCCGTGGCTAGGGCTCTAAGAGTGCTCGAGCCCAATGGGTTGGAGATTGAGACCAAGCTTATTGGGGTTTTGAGGGACATGGTGGCCCTGCAGGCGGGCTATCTTAAGCCCGTGAAGCACAGGCCCAAGTTGTTGAAGAAAGGGAAGGAGGAACAGAGTCTCAAGGATGAGACTCATGAGAGTAGTGAGAGTGAGAGTGGTGAGAGTTCCTTGATATTTGGTGAAGGTCATTTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

269

Amino Acids

29.42

Weight (kDa)

8.19

Isoelectric Point (pI)

66.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 23 - 220 8.9e-40 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013032)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41750
fragaria_vesca FvH4_6g23860
prunus_persica Prupe.7G059600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0482541
rosa_laevigata RLG00000023346
rosa_multiflora Rmu_sc0002969.1_g000012 Rmu_sc0005613.1_g000004
rosa_roxburghii Rroxscaffold_6G00399180 Rroxscaffold_6G00399300
rosa_rugosa Rorug03G0196800
rosa_samantha Rh3AG248300 Rh3BG283300 Rh3CG279900 Rh3DG276200 Rh3DG276300 Rh4BG263400
rosa_wichuraiana Rw0G013130 Rw3G022360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 277
AccI GTMKAC 1 cut(s) 33
AccII CGCG 4 cut(s) 58, 398, 413, 458
AccIII TCCGGA 1 cut(s) 354
AclWI GGATC 2 cut(s) 352, 365
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 1 cut(s) 559
AfiI CCNNNNNNNGG 6 cut(s) 262, 354, 373, 545, 576, 661
AflII CTTAAG 1 cut(s) 671
AgsI TTSAA 1 cut(s) 703
AjiI CACGTC 1 cut(s) 402
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 3 cut(s) 8, 193, 625
AluI AGCT 3 cut(s) 8, 193, 625
Alw21I GWGCWC 1 cut(s) 594
Alw26I GTCTC 5 cut(s) 123, 356, 611, 731, 731
AlwI GGATC 2 cut(s) 352, 365
AlwNI CAGNNNCTG 1 cut(s) 84
Ama87I CYCGRG 1 cut(s) 593
Aor13HI TCCGGA 1 cut(s) 354
AoxI GGCC 8 cut(s) 62, 92, 126, 225, 265, 567, 651, 689
ApeKI GCWGC 2 cut(s) 78, 184
ApoI RAATTY 1 cut(s) 452
ArsI GACNNNNNNTTYG 1 cut(s) 37
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 7 cut(s) 69, 93, 127, 208, 265, 652, 690
AsuC2I CCSGG 2 cut(s) 125, 264
AsuHPI GGTGA 7 cut(s) 158, 282, 321, 436, 478, 779, 800
AsuII TTCGAA 1 cut(s) 24
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 2 cut(s) 69, 208
BalI TGGCCA 1 cut(s) 227
BamHI GGATCC 1 cut(s) 357
BanII GRGCYC 2 cut(s) 583, 600
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 594
BbvI GCAGC 2 cut(s) 90, 196
BccI CCATC 6 cut(s) 80, 143, 177, 248, 308, 415
BceAI ACGGC 3 cut(s) 34, 523, 554
BciT130I CCWGG 1 cut(s) 224
BcnI CCSGG 2 cut(s) 125, 264
BcoDI GTCTC 5 cut(s) 123, 356, 611, 731, 731
BfaI CTAG 1 cut(s) 576
BfmI CTRYAG 2 cut(s) 76, 656
BfrI CTTAAG 1 cut(s) 671
BglI GCCNNNNNGGC 2 cut(s) 61, 659
BisI GCNGC 6 cut(s) 53, 56, 79, 185, 250, 253
BlsI GCNGC 6 cut(s) 54, 57, 80, 186, 251, 254
Bme1390I CCNGG 3 cut(s) 125, 224, 264
Bme18I GGWCC 2 cut(s) 69, 208
BmeT110I CYCGRG 1 cut(s) 593
BmgBI CACGTC 1 cut(s) 402
BmgT120I GGNCC 7 cut(s) 69, 93, 127, 208, 265, 652, 690
BmiI GGNNCC 3 cut(s) 359, 443, 537
BmrFI CCNGG 3 cut(s) 125, 224, 264
BmrI ACTGGG 1 cut(s) 95
BmsI GCATC 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 18
Bpu14I TTCGAA 1 cut(s) 24
BpuEI CTTGAG 2 cut(s) 242, 713
BpuMI CCSGG 2 cut(s) 125, 264
BsaI GGTCTC 1 cut(s) 611
BsaJI CCNNGG 4 cut(s) 56, 65, 561, 570
BsaWI WCCGGW 1 cut(s) 354
BsaXI ACNNNNNCTCC 4 cut(s) 179, 209, 454, 484
Bsc4I CCNNNNNNNGG 6 cut(s) 262, 354, 373, 545, 576, 661
Bse118I RCCGGY 3 cut(s) 90, 205, 373
Bse1I ACTGG 2 cut(s) 101, 367
BseAI TCCGGA 1 cut(s) 354
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 4 cut(s) 56, 65, 561, 570
BseGI GGATG 6 cut(s) 135, 169, 481, 487, 493, 739
BseLI CCNNNNNNNGG 6 cut(s) 262, 354, 373, 545, 576, 661
BseMII CTCAG 1 cut(s) 518
BseNI ACTGG 2 cut(s) 101, 367
BseRI GAGGAG 2 cut(s) 269, 475
BseXI GCAGC 2 cut(s) 90, 196
BseYI CCCAGC 3 cut(s) 95, 129, 362
Bsh1236I CGCG 4 cut(s) 58, 398, 413, 458
BshFI GGCC 8 cut(s) 64, 94, 128, 227, 267, 569, 653, 691
BsiHKAI GWGCWC 1 cut(s) 594
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 6 cut(s) 91, 125, 206, 263, 355, 374
BslFI GGGAC 2 cut(s) 256, 656
BslI CCNNNNNNNGG 6 cut(s) 262, 354, 373, 545, 576, 661
BsmAI GTCTC 5 cut(s) 123, 356, 611, 731, 731
BsmBI CGTCTC 1 cut(s) 356
BsmFI GGGAC 2 cut(s) 256, 656
BsnI GGCC 8 cut(s) 64, 94, 128, 227, 267, 569, 653, 691
Bso31I GGTCTC 1 cut(s) 611
BsoBI CYCGRG 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 24
Bsp1286I GDGCHC 3 cut(s) 583, 594, 600
Bsp13I TCCGGA 1 cut(s) 354
Bsp143I GATC 1 cut(s) 357
Bsp19I CCATGG 1 cut(s) 561
Bsp68I TCGCGA 1 cut(s) 458
BspANI GGCC 8 cut(s) 64, 94, 128, 227, 267, 569, 653, 691
BspCNI CTCAG 1 cut(s) 519
BspEI TCCGGA 1 cut(s) 354
BspFNI CGCG 4 cut(s) 58, 398, 413, 458
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 3 cut(s) 359, 443, 537
BspMAI CTGCAG 2 cut(s) 80, 660
BspPI GGATC 2 cut(s) 352, 365
BspT104I TTCGAA 1 cut(s) 24
BspTI CTTAAG 1 cut(s) 671
BspTNI GGTCTC 1 cut(s) 611
BsrBI CCGCTC 1 cut(s) 277
BsrFI RCCGGY 3 cut(s) 90, 205, 373
BsrI ACTGG 2 cut(s) 101, 367
BssAI RCCGGY 3 cut(s) 90, 205, 373
BssECI CCNNGG 4 cut(s) 56, 65, 561, 570
BssMI GATC 1 cut(s) 357
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 3 cut(s) 31, 86, 333
Bst6I CTCTTC 2 cut(s) 52, 314
BstAFI CTTAAG 1 cut(s) 671
BstBI TTCGAA 1 cut(s) 24
BstC8I GCNNGC 4 cut(s) 411, 433, 660, 664
BstDEI CTNAG 2 cut(s) 527, 584
BstDSI CCRYGG 3 cut(s) 56, 561, 570
BstF5I GGATG 6 cut(s) 135, 169, 481, 487, 493, 739
BstFNI CGCG 4 cut(s) 58, 398, 413, 458
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 1 cut(s) 360
BstMAI GTCTC 5 cut(s) 123, 356, 611, 731, 731
BstMBI GATC 1 cut(s) 357
BstMWI GCNNNNNNNGC 6 cut(s) 61, 105, 190, 395, 659, 682
BstNI CCWGG 1 cut(s) 224
BstSCI CCNGG 3 cut(s) 123, 222, 262
BstSFI CTRYAG 2 cut(s) 76, 656
BstUI CGCG 4 cut(s) 58, 398, 413, 458
BstV1I GCAGC 2 cut(s) 90, 196
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 357
BstXI CCANNNNNNTGG 1 cut(s) 607
BstYI RGATCY 1 cut(s) 357
BsuRI GGCC 8 cut(s) 64, 94, 128, 227, 267, 569, 653, 691
BtgI CCRYGG 3 cut(s) 56, 561, 570
BtrI CACGTC 1 cut(s) 402
BtsCI GGATG 6 cut(s) 135, 169, 481, 487, 493, 739
BtuMI TCGCGA 1 cut(s) 458
Cac8I GCNNGC 4 cut(s) 411, 433, 660, 664
CaiI CAGNNNCTG 1 cut(s) 84
CciI TCATGA 1 cut(s) 742
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 3 cut(s) 90, 205, 373
Cfr13I GGNCC 7 cut(s) 69, 93, 127, 208, 265, 652, 690
Cfr42I CCGCGG 1 cut(s) 59
CseI GACGC 1 cut(s) 404
Csp6I GTAC 1 cut(s) 558
CviAII CATG 3 cut(s) 562, 646, 743
CviQI GTAC 1 cut(s) 558
DdeI CTNAG 2 cut(s) 527, 584
DpnI GATC 1 cut(s) 359
DpnII GATC 1 cut(s) 357
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 52, 314
EarI CTCTTC 2 cut(s) 52, 314
EciI GGCGGA 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 561
Eco24I GRGCYC 2 cut(s) 583, 600
Eco31I GGTCTC 1 cut(s) 611
Eco47I GGWCC 2 cut(s) 69, 208
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 593
EcoRII CCWGG 1 cut(s) 222
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 2 cut(s) 583, 600
ErhI CCWWGG 1 cut(s) 561
Esp3I CGTCTC 1 cut(s) 356
FaeI CATG 3 cut(s) 565, 649, 746
FaiI YATR 4 cut(s) 158, 563, 647, 744
FaqI GGGAC 2 cut(s) 256, 656
FatI CATG 3 cut(s) 561, 645, 742
FauI CCCGC 3 cut(s) 282, 321, 655
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 6 cut(s) 53, 56, 79, 185, 250, 253
FokI GGATG 6 cut(s) 122, 156, 488, 494, 500, 746
FriOI GRGCYC 2 cut(s) 583, 600
Fsp4HI GCNGC 6 cut(s) 53, 56, 79, 185, 250, 253
FspBI CTAG 1 cut(s) 576
GlaI GCGC 1 cut(s) 183
GluI GCNGC 6 cut(s) 53, 56, 79, 185, 250, 253
GsaI CCCAGC 3 cut(s) 99, 133, 366
HaeIII GGCC 8 cut(s) 64, 94, 128, 227, 267, 569, 653, 691
HapII CCGG 6 cut(s) 91, 125, 206, 263, 355, 374
HgaI GACGC 1 cut(s) 404
HhaI GCGC 1 cut(s) 184
Hin1II CATG 3 cut(s) 565, 649, 746
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 4 cut(s) 512, 523, 724, 739
HpaII CCGG 6 cut(s) 91, 125, 206, 263, 355, 374
HphI GGTGA 7 cut(s) 158, 282, 321, 436, 478, 779, 800
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 2 cut(s) 517, 528
Hpy188III TCNNGA 5 cut(s) 176, 211, 355, 457, 743
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 4 cut(s) 20, 337, 398, 403
HpyAV CCTTC 6 cut(s) 173, 303, 409, 526, 706, 785
HpyCH4III ACNGT 3 cut(s) 31, 86, 333
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 3 cut(s) 78, 114, 658
HpyF10VI GCNNNNNNNGC 6 cut(s) 61, 105, 190, 395, 659, 682
HpyF3I CTNAG 2 cut(s) 527, 584
HpySE526I ACGT 1 cut(s) 401
Hsp92II CATG 3 cut(s) 565, 649, 746
HspAI GCGC 1 cut(s) 182
Kpn2I TCCGGA 1 cut(s) 354
KspI CCGCGG 1 cut(s) 59
Kzo9I GATC 1 cut(s) 357
LmnI GCTCC 3 cut(s) 282, 501, 535
Lsp1109I GCAGC 2 cut(s) 90, 196
LweI GCATC 1 cut(s) 513
MaeI CTAG 1 cut(s) 576
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 1 cut(s) 86
MalI GATC 1 cut(s) 359
MbiI CCGCTC 1 cut(s) 277
MboI GATC 1 cut(s) 357
MboII GAAGA 4 cut(s) 23, 39, 301, 715
MflI RGATCY 1 cut(s) 357
MhlI GDGCHC 3 cut(s) 583, 594, 600
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 1 cut(s) 452
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 3 cut(s) 506, 733, 733
MmeI TCCRAC 1 cut(s) 588
Mox20I TGGCCA 1 cut(s) 227
MroI TCCGGA 1 cut(s) 354
MscI TGGCCA 1 cut(s) 227
MseI TTAA 1 cut(s) 672
Msp20I TGGCCA 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 58
MspCI CTTAAG 1 cut(s) 671
MspI CCGG 6 cut(s) 91, 125, 206, 263, 355, 374
MspR9I CCNGG 3 cut(s) 125, 224, 264
MvaI CCWGG 1 cut(s) 224
MvnI CGCG 4 cut(s) 58, 398, 413, 458
MwoI GCNNNNNNNGC 6 cut(s) 61, 105, 190, 395, 659, 682
NciI CCSGG 2 cut(s) 125, 264
NcoI CCATGG 1 cut(s) 561
NdeII GATC 1 cut(s) 357
NlaIII CATG 3 cut(s) 565, 649, 746
NlaIV GGNNCC 3 cut(s) 359, 443, 537
NmeAIII GCCGAG 1 cut(s) 90
NruI TCGCGA 1 cut(s) 458
NspV TTCGAA 1 cut(s) 24
PaeR7I CTCGAG 1 cut(s) 593
PagI TCATGA 1 cut(s) 742
PcsI WCGNNNNNNNCGW 3 cut(s) 21, 390, 455
PfeI GAWTC 1 cut(s) 523
PkrI GCNGC 6 cut(s) 54, 57, 80, 186, 251, 254
PleI GAGTC 3 cut(s) 506, 732, 733
PpsI GAGTC 3 cut(s) 506, 732, 733
Psp6I CCWGG 1 cut(s) 222
PspFI CCCAGC 3 cut(s) 95, 129, 362
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 3 cut(s) 359, 443, 537
PspPI GGNCC 7 cut(s) 69, 93, 127, 208, 265, 652, 690
PspXI VCTCGAGB 1 cut(s) 593
PstI CTGCAG 2 cut(s) 80, 660
PstNI CAGNNNCTG 1 cut(s) 84
PsuI RGATCY 1 cut(s) 357
RruI TCGCGA 1 cut(s) 458
RsaI GTAC 1 cut(s) 559
RsaNI GTAC 1 cut(s) 558
SacII CCGCGG 1 cut(s) 59
SaqAI TTAA 1 cut(s) 672
SatI GCNGC 6 cut(s) 53, 56, 79, 185, 250, 253
Sau3AI GATC 1 cut(s) 357
Sau96I GGNCC 7 cut(s) 69, 93, 127, 208, 265, 652, 690
SbfI CCTGCAGG 1 cut(s) 660
SchI GAGTC 3 cut(s) 506, 733, 733
ScrFI CCNGG 3 cut(s) 125, 224, 264
SdaI CCTGCAGG 1 cut(s) 660
SduI GDGCHC 3 cut(s) 583, 594, 600
SetI ASST 9 cut(s) 10, 47, 71, 195, 295, 309, 404, 627, 796
SfaNI GCATC 1 cut(s) 513
SfcI CTRYAG 2 cut(s) 76, 656
Sfr274I CTCGAG 1 cut(s) 593
Sfr303I CCGCGG 1 cut(s) 59
SfuI TTCGAA 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 373
SgrBI CCGCGG 1 cut(s) 59
SinI GGWCC 2 cut(s) 69, 208
SlaI CTCGAG 1 cut(s) 593
SmlI CTYRAG 4 cut(s) 257, 593, 671, 728
SmoI CTYRAG 4 cut(s) 257, 593, 671, 728
Sse8387I CCTGCAGG 1 cut(s) 660
Sse9I AATT 1 cut(s) 452
SspMI CTAG 1 cut(s) 576
StyD4I CCNGG 3 cut(s) 123, 222, 262
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 3 cut(s) 31, 86, 333
TaiI ACGT 1 cut(s) 404
TaqI TCGA 6 cut(s) 24, 282, 320, 393, 449, 594
TaqII GACCGA 1 cut(s) 325
TasI AATT 1 cut(s) 452
TauI GCSGC 4 cut(s) 55, 58, 252, 255
TfiI GAWTC 1 cut(s) 523
Tru1I TTAA 1 cut(s) 672
Tru9I TTAA 1 cut(s) 672
TseI GCWGC 2 cut(s) 78, 184
Vha464I CTTAAG 1 cut(s) 671
VpaK11BI GGWCC 2 cut(s) 69, 208
XapI RAATTY 1 cut(s) 452
XcmI CCANNNNNNNNNTGG 1 cut(s) 568
XhoI CTCGAG 1 cut(s) 593
XmiI GTMKAC 1 cut(s) 33
XspI CTAG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.