Rroxscaffold_6G00399300

DTW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013032
Physical Location & Seq
Reverse (-)
21247137 .. 21248196
1060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00399300.1

Sequence Viewer

Length: 786 bp
ATGGAAGCTGAAGACGACGAATTCGAAAACGCCTACAACGCCACCTCTTCGCCGCCGCGGAGGCCAAGGTCCATCTGCGGCAACTGTAACCGGCCCAGCCCAGTCTGCCTCTGCAAGTCTCTCCCGGCCCGGCCCATCCCAACCCAAACGCAAATCATAATCCTCCACCATCCTCACGAGGCGCAGCACAAGCTCTCCACCACGCCGGTCCTGACAAAATGCCTGGCCAACTCCACCGCCATCGTCAGCCGCCGCCTCAAGCCGGGCCTGTCCCCGCTCCTCGACCAATCACCTTCCGCCATCTACCTCTTCCCGCCCTCGAAATCCTCACCGTCCGTCAGTTTATCCCGTCTCCGGATCCCGGACCAGTCGGCGCCGGTGGTGGTCGTAGCTTTCGACGCGACGTGGAAGCACGCGAAGGAGATGGTGAGGGCGAGCGAGGGGTTCCTGTCGAAATTCGCGAGGAGGGTGAGTTTGGATGTGGATGAGGATGTGAGGGGAGGGAGCATCTACGACTCGGAGTTGATTCTGAGGAAGGAGCCGTTTGGTGGGTGCGTGAGTACCATGGAGGCCGTGGCTAGGGCTCTAAGAGTGCTCGAGCCCAATGGGTTGGAGATTGAGAGCAAGCTTATTCGGGTTTTGAGGGACATGGTGGCTTTGCAGGCGGGTTATCTTAAGCCTGTGAAGCCCAGGCCCAAGTTGTTGAAGAAAGGGAAGGAAACACAGAGGCCCAAGAATGAGACTCATGAGAGTAGTGAGGGTTCCCCGATAGTTGGTGAAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

261

Amino Acids

28.68

Weight (kDa)

9.25

Isoelectric Point (pI)

60.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 23 - 221 7e-40 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013032)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41750
fragaria_vesca FvH4_6g23860
prunus_persica Prupe.7G059600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0482541
rosa_laevigata RLG00000023346
rosa_multiflora Rmu_sc0002969.1_g000012 Rmu_sc0005613.1_g000004
rosa_roxburghii Rroxscaffold_6G00399180 Rroxscaffold_6G00399300
rosa_rugosa Rorug03G0196800
rosa_samantha Rh3AG248300 Rh3BG283300 Rh3CG279900 Rh3DG276200 Rh3DG276300 Rh4BG263400
rosa_wichuraiana Rw0G013130 Rw3G022360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 373
AccBSI CCGCTC 1 cut(s) 277
AccII CGCG 4 cut(s) 58, 401, 416, 461
AccIII TCCGGA 1 cut(s) 354
AclWI GGATC 2 cut(s) 352, 365
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 2 cut(s) 20, 455
AcuI CTGAAG 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 374
AfaI GTAC 1 cut(s) 562
AfiI CCNNNNNNNGG 6 cut(s) 262, 354, 361, 548, 579, 773
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 1 cut(s) 706
AjiI CACGTC 1 cut(s) 405
AjnI CCWGG 2 cut(s) 222, 689
AluBI AGCT 4 cut(s) 8, 193, 392, 628
AluI AGCT 4 cut(s) 8, 193, 392, 628
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 3 cut(s) 123, 356, 734
AlwI GGATC 2 cut(s) 352, 365
Ama87I CYCGRG 1 cut(s) 596
Aor13HI TCCGGA 1 cut(s) 354
AoxI GGCC 9 cut(s) 62, 92, 126, 131, 225, 265, 570, 692, 728
ApeKI GCWGC 1 cut(s) 184
ApoI RAATTY 2 cut(s) 20, 455
ArsI GACNNNNNNTTYG 1 cut(s) 37
AspLEI GCGC 2 cut(s) 184, 376
AspS9I GGNCC 9 cut(s) 69, 93, 127, 132, 208, 265, 364, 693, 729
AsuC2I CCSGG 4 cut(s) 125, 130, 264, 362
AsuHPI GGTGA 4 cut(s) 282, 321, 439, 481
AsuII TTCGAA 1 cut(s) 24
AvaI CYCGRG 1 cut(s) 596
AvaII GGWCC 3 cut(s) 69, 208, 364
BalI TGGCCA 1 cut(s) 227
BamHI GGATCC 1 cut(s) 357
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 2 cut(s) 586, 603
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 597
BbvI GCAGC 1 cut(s) 196
BccI CCATC 6 cut(s) 80, 143, 177, 248, 308, 418
BceAI ACGGC 2 cut(s) 526, 557
BciT130I CCWGG 2 cut(s) 224, 691
BcnI CCSGG 4 cut(s) 125, 130, 264, 362
BcoDI GTCTC 3 cut(s) 123, 356, 734
BfaI CTAG 1 cut(s) 579
BfoI RGCGCY 1 cut(s) 377
BfrI CTTAAG 1 cut(s) 674
BglI GCCNNNNNGGC 1 cut(s) 61
BisI GCNGC 6 cut(s) 53, 56, 79, 185, 250, 253
BlsI GCNGC 6 cut(s) 54, 57, 80, 186, 251, 254
Bme1390I CCNGG 6 cut(s) 125, 130, 224, 264, 362, 691
Bme18I GGWCC 3 cut(s) 69, 208, 364
BmeT110I CYCGRG 1 cut(s) 596
BmgBI CACGTC 1 cut(s) 405
BmgT120I GGNCC 9 cut(s) 69, 93, 127, 132, 208, 265, 364, 693, 729
BmiI GGNNCC 5 cut(s) 359, 375, 446, 540, 763
BmrFI CCNGG 6 cut(s) 125, 130, 224, 264, 362, 691
BmrI ACTGGG 1 cut(s) 95
BmsI GCATC 1 cut(s) 516
BmuI ACTGGG 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 18
Bpu14I TTCGAA 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 4 cut(s) 125, 130, 264, 362
BsaHI GRCGYC 1 cut(s) 374
BsaJI CCNNGG 5 cut(s) 56, 65, 564, 573, 689
BsaWI WCCGGW 1 cut(s) 354
BsaXI ACNNNNNCTCC 4 cut(s) 179, 209, 457, 487
Bsc4I CCNNNNNNNGG 6 cut(s) 262, 354, 361, 548, 579, 773
Bse118I RCCGGY 3 cut(s) 90, 205, 376
Bse1I ACTGG 2 cut(s) 101, 367
BseAI TCCGGA 1 cut(s) 354
BseBI CCWGG 2 cut(s) 224, 691
BseDI CCNNGG 5 cut(s) 56, 65, 564, 573, 689
BseGI GGATG 5 cut(s) 135, 169, 484, 490, 496
BseLI CCNNNNNNNGG 6 cut(s) 262, 354, 361, 548, 579, 773
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 2 cut(s) 101, 367
BseRI GAGGAG 2 cut(s) 269, 478
BseXI GCAGC 1 cut(s) 196
BseYI CCCAGC 1 cut(s) 95
Bsh1236I CGCG 4 cut(s) 58, 401, 416, 461
BshFI GGCC 9 cut(s) 64, 94, 128, 133, 227, 267, 572, 694, 730
BshNI GGYRCC 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 597
BsiHKCI CYCGRG 1 cut(s) 596
BsiSI CCGG 8 cut(s) 91, 125, 130, 206, 263, 355, 362, 377
BslFI GGGAC 2 cut(s) 256, 659
BslI CCNNNNNNNGG 6 cut(s) 262, 354, 361, 548, 579, 773
BsmAI GTCTC 3 cut(s) 123, 356, 734
BsmBI CGTCTC 1 cut(s) 356
BsmFI GGGAC 2 cut(s) 256, 659
BsnI GGCC 9 cut(s) 64, 94, 128, 133, 227, 267, 572, 694, 730
BsoBI CYCGRG 1 cut(s) 596
Bsp119I TTCGAA 1 cut(s) 24
Bsp1286I GDGCHC 3 cut(s) 586, 597, 603
Bsp13I TCCGGA 1 cut(s) 354
Bsp143I GATC 1 cut(s) 357
Bsp19I CCATGG 1 cut(s) 564
Bsp68I TCGCGA 1 cut(s) 461
BspANI GGCC 9 cut(s) 64, 94, 128, 133, 227, 267, 572, 694, 730
BspCNI CTCAG 1 cut(s) 522
BspEI TCCGGA 1 cut(s) 354
BspFNI CGCG 4 cut(s) 58, 401, 416, 461
BspHI TCATGA 1 cut(s) 745
BspLI GGNNCC 5 cut(s) 359, 375, 446, 540, 763
BspPI GGATC 2 cut(s) 352, 365
BspT104I TTCGAA 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 373
BspTI CTTAAG 1 cut(s) 674
BsrBI CCGCTC 1 cut(s) 277
BsrFI RCCGGY 3 cut(s) 90, 205, 376
BsrI ACTGG 2 cut(s) 101, 367
BssAI RCCGGY 3 cut(s) 90, 205, 376
BssECI CCNNGG 5 cut(s) 56, 65, 564, 573, 689
BssMI GATC 1 cut(s) 357
BssNI GRCGYC 1 cut(s) 374
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 2 cut(s) 65, 564
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 2 cut(s) 224, 691
Bst4CI ACNGT 2 cut(s) 86, 333
Bst6I CTCTTC 2 cut(s) 52, 314
BstACI GRCGYC 1 cut(s) 374
BstAFI CTTAAG 1 cut(s) 674
BstBI TTCGAA 1 cut(s) 24
BstC8I GCNNGC 4 cut(s) 414, 436, 626, 663
BstDEI CTNAG 2 cut(s) 530, 587
BstDSI CCRYGG 3 cut(s) 56, 564, 573
BstF5I GGATG 5 cut(s) 135, 169, 484, 490, 496
BstFNI CGCG 4 cut(s) 58, 401, 416, 461
BstH2I RGCGCY 1 cut(s) 377
BstHHI GCGC 2 cut(s) 184, 376
BstKTI GATC 1 cut(s) 360
BstMAI GTCTC 3 cut(s) 123, 356, 734
BstMBI GATC 1 cut(s) 357
BstMWI GCNNNNNNNGC 7 cut(s) 38, 61, 105, 190, 398, 662, 685
BstNI CCWGG 2 cut(s) 224, 691
BstSCI CCNGG 6 cut(s) 123, 128, 222, 262, 360, 689
BstUI CGCG 4 cut(s) 58, 401, 416, 461
BstV1I GCAGC 1 cut(s) 196
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 357
BstXI CCANNNNNNTGG 1 cut(s) 610
BstYI RGATCY 1 cut(s) 357
BsuRI GGCC 9 cut(s) 64, 94, 128, 133, 227, 267, 572, 694, 730
BtgI CCRYGG 3 cut(s) 56, 564, 573
BtrI CACGTC 1 cut(s) 405
BtsCI GGATG 5 cut(s) 135, 169, 484, 490, 496
BtuMI TCGCGA 1 cut(s) 461
Cac8I GCNNGC 4 cut(s) 414, 436, 626, 663
CciI TCATGA 1 cut(s) 745
CfoI GCGC 2 cut(s) 184, 376
Cfr10I RCCGGY 3 cut(s) 90, 205, 376
Cfr13I GGNCC 9 cut(s) 69, 93, 127, 132, 208, 265, 364, 693, 729
Cfr42I CCGCGG 1 cut(s) 59
CseI GACGC 1 cut(s) 407
Csp6I GTAC 1 cut(s) 561
CviAII CATG 3 cut(s) 565, 649, 746
CviQI GTAC 1 cut(s) 561
DdeI CTNAG 2 cut(s) 530, 587
DinI GGCGCC 1 cut(s) 375
DpnI GATC 1 cut(s) 359
DpnII GATC 1 cut(s) 357
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 52, 314
EarI CTCTTC 2 cut(s) 52, 314
EciI GGCGGA 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 65, 564
Eco24I GRGCYC 2 cut(s) 586, 603
Eco47I GGWCC 3 cut(s) 69, 208, 364
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 596
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 222, 689
EcoT14I CCWWGG 2 cut(s) 65, 564
EcoT38I GRGCYC 2 cut(s) 586, 603
EgeI GGCGCC 1 cut(s) 375
EheI GGCGCC 1 cut(s) 375
ErhI CCWWGG 2 cut(s) 65, 564
Esp3I CGTCTC 1 cut(s) 356
FaeI CATG 3 cut(s) 568, 652, 749
FaiI YATR 4 cut(s) 158, 566, 650, 747
FaqI GGGAC 2 cut(s) 256, 659
FatI CATG 3 cut(s) 564, 648, 745
FauI CCCGC 3 cut(s) 282, 321, 658
Fnu4HI GCNGC 6 cut(s) 53, 56, 79, 185, 250, 253
FokI GGATG 5 cut(s) 122, 156, 491, 497, 503
FriOI GRGCYC 2 cut(s) 586, 603
Fsp4HI GCNGC 6 cut(s) 53, 56, 79, 185, 250, 253
FspBI CTAG 1 cut(s) 579
GlaI GCGC 2 cut(s) 183, 375
GluI GCNGC 6 cut(s) 53, 56, 79, 185, 250, 253
GsaI CCCAGC 1 cut(s) 99
HaeII RGCGCY 1 cut(s) 377
HaeIII GGCC 9 cut(s) 64, 94, 128, 133, 227, 267, 572, 694, 730
HapII CCGG 8 cut(s) 91, 125, 130, 206, 263, 355, 362, 377
HgaI GACGC 1 cut(s) 407
HhaI GCGC 2 cut(s) 184, 376
Hin1I GRCGYC 1 cut(s) 374
Hin1II CATG 3 cut(s) 568, 652, 749
Hin6I GCGC 2 cut(s) 182, 374
HinP1I GCGC 2 cut(s) 182, 374
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 3 cut(s) 515, 526, 742
HpaII CCGG 8 cut(s) 91, 125, 130, 206, 263, 355, 362, 377
HphI GGTGA 4 cut(s) 282, 321, 439, 481
Hpy188I TCNGA 2 cut(s) 520, 531
Hpy188III TCNNGA 5 cut(s) 176, 211, 355, 460, 746
Hpy99I CGWCG 3 cut(s) 20, 401, 406
HpyAV CCTTC 5 cut(s) 303, 412, 529, 709, 773
HpyCH4III ACNGT 2 cut(s) 86, 333
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 2 cut(s) 114, 661
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 61, 105, 190, 398, 662, 685
HpyF3I CTNAG 2 cut(s) 530, 587
HpySE526I ACGT 1 cut(s) 404
Hsp92I GRCGYC 1 cut(s) 374
Hsp92II CATG 3 cut(s) 568, 652, 749
HspAI GCGC 2 cut(s) 182, 374
KasI GGCGCC 1 cut(s) 373
Kpn2I TCCGGA 1 cut(s) 354
KspI CCGCGG 1 cut(s) 59
Kzo9I GATC 1 cut(s) 357
LmnI GCTCC 3 cut(s) 282, 504, 538
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 1 cut(s) 516
MaeI CTAG 1 cut(s) 579
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 86
MalI GATC 1 cut(s) 359
MbiI CCGCTC 1 cut(s) 277
MboI GATC 1 cut(s) 357
MboII GAAGA 4 cut(s) 23, 39, 301, 718
MflI RGATCY 1 cut(s) 357
MhlI GDGCHC 3 cut(s) 586, 597, 603
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 2 cut(s) 20, 455
MluNI TGGCCA 1 cut(s) 227
Mly113I GGCGCC 1 cut(s) 374
MlyI GAGTC 2 cut(s) 509, 736
MmeI TCCRAC 1 cut(s) 591
Mox20I TGGCCA 1 cut(s) 227
MroI TCCGGA 1 cut(s) 354
MscI TGGCCA 1 cut(s) 227
MseI TTAA 1 cut(s) 675
Msp20I TGGCCA 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 58
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 8 cut(s) 91, 125, 130, 206, 263, 355, 362, 377
MspR9I CCNGG 6 cut(s) 125, 130, 224, 264, 362, 691
MvaI CCWGG 2 cut(s) 224, 691
MvnI CGCG 4 cut(s) 58, 401, 416, 461
MwoI GCNNNNNNNGC 7 cut(s) 38, 61, 105, 190, 398, 662, 685
NarI GGCGCC 1 cut(s) 374
NciI CCSGG 4 cut(s) 125, 130, 264, 362
NcoI CCATGG 1 cut(s) 564
NdeII GATC 1 cut(s) 357
NlaIII CATG 3 cut(s) 568, 652, 749
NlaIV GGNNCC 5 cut(s) 359, 375, 446, 540, 763
NruI TCGCGA 1 cut(s) 461
NspV TTCGAA 1 cut(s) 24
PaeR7I CTCGAG 1 cut(s) 596
PagI TCATGA 1 cut(s) 745
PcsI WCGNNNNNNNCGW 3 cut(s) 21, 393, 458
PfeI GAWTC 1 cut(s) 526
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 6 cut(s) 54, 57, 80, 186, 251, 254
PleI GAGTC 2 cut(s) 509, 736
PluTI GGCGCC 1 cut(s) 377
PpsI GAGTC 2 cut(s) 509, 736
Psp6I CCWGG 2 cut(s) 222, 689
PspFI CCCAGC 1 cut(s) 95
PspGI CCWGG 2 cut(s) 222, 689
PspN4I GGNNCC 5 cut(s) 359, 375, 446, 540, 763
PspPI GGNCC 9 cut(s) 69, 93, 127, 132, 208, 265, 364, 693, 729
PspXI VCTCGAGB 1 cut(s) 596
PsrI GAACNNNNNNTAC 2 cut(s) 745, 777
PsuI RGATCY 1 cut(s) 357
RruI TCGCGA 1 cut(s) 461
RsaI GTAC 1 cut(s) 562
RsaNI GTAC 1 cut(s) 561
SacII CCGCGG 1 cut(s) 59
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 6 cut(s) 53, 56, 79, 185, 250, 253
Sau3AI GATC 1 cut(s) 357
Sau96I GGNCC 9 cut(s) 69, 93, 127, 132, 208, 265, 364, 693, 729
SchI GAGTC 2 cut(s) 509, 736
ScrFI CCNGG 6 cut(s) 125, 130, 224, 264, 362, 691
SduI GDGCHC 3 cut(s) 586, 597, 603
SetI ASST 9 cut(s) 10, 47, 71, 195, 295, 309, 394, 407, 630
SfaNI GCATC 1 cut(s) 516
SfoI GGCGCC 1 cut(s) 375
Sfr274I CTCGAG 1 cut(s) 596
Sfr303I CCGCGG 1 cut(s) 59
SfuI TTCGAA 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 376
SgrBI CCGCGG 1 cut(s) 59
SinI GGWCC 3 cut(s) 69, 208, 364
SlaI CTCGAG 1 cut(s) 596
SmlI CTYRAG 3 cut(s) 257, 596, 674
SmoI CTYRAG 3 cut(s) 257, 596, 674
Sse9I AATT 2 cut(s) 20, 455
SspDI GGCGCC 1 cut(s) 373
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 6 cut(s) 123, 128, 222, 262, 360, 689
StyI CCWWGG 2 cut(s) 65, 564
TaaI ACNGT 2 cut(s) 86, 333
TaiI ACGT 1 cut(s) 407
TaqI TCGA 6 cut(s) 24, 282, 320, 396, 452, 597
TasI AATT 2 cut(s) 20, 455
TauI GCSGC 5 cut(s) 55, 58, 81, 252, 255
TfiI GAWTC 1 cut(s) 526
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TseI GCWGC 1 cut(s) 184
TspGWI ACGGA 1 cut(s) 325
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 3 cut(s) 69, 208, 364
XapI RAATTY 2 cut(s) 20, 455
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XhoI CTCGAG 1 cut(s) 596
XspI CTAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.