RLG00000026127

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005513
Physical Location & Seq
Reverse (-)
685488 .. 689662
4175 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026127

Sequence Viewer

Length: 1239 bp
ATGGCATTCGCAACAGATAGTAGGGTTCCCATGATCGTGCTACTAGTACTAGTGCTAGCAGCAGCAGCTCTTGCTCATAGTGAAGGTGATGATCATGCAACGCCTCCGATTCGGTCTCCAAATCCATTGTCGCCATCATCAGATGGTTCGGGTTCTGCTGAACCGGAGGGTTTAGTCGACGTAGGTCTATGTGCCATTCAATGTGGTGCCAAGTGTTTTGGTCGCAAGGCATCCCTGATTTCACCTGATAAGTTTCGCTTTCCCAAAATTCATTTTGGTGGAGCTTTTTGTTTTGGTACATGCATGCTAGAGTGCACCATGACCCAGGGGAATGTGAAGCCAGAGCTCTATCATTGTACACTTGGATGTGCTTATAAGTTTGCTGCTAAAACAAAATTAACTGGTTCAGATGCTCATAAAGGGAATAGCCATTTGGGTTCTTGCCACAACAATTGGACTTATAAGATCGGGCTGGCCAACGGGGAGGCTGAGATGAACTTTACTGTGAGTCAGGTGGGGTCGGATTTTAGCCTATTGGGCCGCGGGCCAAATGATAATCCATTAGTGCTAGATACAAACTACCCAATAGCCAAAACTGGTATTGTGGCCCAAATTGGGTCCGGTTCTGGCCCAGTTGTTGCTCTTCGGTCTGACATGGACGCCCTCCCTCTGCAAGAGCACAAGAGTATGATTGATGGGATAATGCACAGTTGTGGTCACGATGCTCACACGACTATGCTTCTAGGCTATGCTAATTTGCTTAGTCAACGGAAAGAGAAACTTCAAGTCAACATATTTCAACCTGCTGAGGAGGGAGGTGCTGGAGCAGCCAAGATGATAAAACAAGGTGCTCTTGGTGAAGCCGAAGCAATATTTGGGATGCACGTTGATTATATGATACCCACAAGAAGCACAGCATCCATGGTAGGCTTCTTTGAAGCAAATATAGTCATCACTATTGACTCAATACTCGCTGCCTCTTTTGCAATTTCAGCATTGCAGCAGCTCATCTCAAGTGAAATTGATCCCCTCCAAAGTCAAGTTCTGTCTGTCACTTATGTTCGAGGAGGGACTGCATCCAATGTAATTCCAGCCTATGTTGAATTTCGGGGCATATTAAGGCTTGTACCAACTCATGTTATTGAAGGGCAGGCAGCTGTGCATAGATGTTTTGCATACATTGACATGAAAAGTGAAAAGTTTCCACCATTGCCTGCTGTTTTCAATGACAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009850 GO:0009987 GO:0010178 GO:0010817 GO:0016787 GO:0016810 GO:0042445 GO:0044237 GO:0065007 GO:0065008
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

413

Amino Acids

43.77

Weight (kDa)

6.15

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 214 - 315 2e-19 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 375, 462
Acc36I ACCTGC 1 cut(s) 811
AccB1I GGYRCC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 177
AccII CGCG 1 cut(s) 543
AciI CCGC 2 cut(s) 541, 543
AclWI GGATC 1 cut(s) 1019
AcoI YGGCCR 1 cut(s) 474
AcsI RAATTY 2 cut(s) 267, 1103
AcyI GRCGYC 1 cut(s) 660
AfaI GTAC 4 cut(s) 48, 298, 358, 1128
AfiI CCNNNNNNNGG 1 cut(s) 615
AgsI TTSAA 7 cut(s) 200, 785, 800, 938, 1103, 1145, 1225
AhlI ACTAGT 2 cut(s) 43, 49
AjnI CCWGG 1 cut(s) 324
AjuI GAANNNNNNNTTGG 6 cut(s) 416, 448, 858, 890, 1026, 1058
AleI CACNNNNGTG 1 cut(s) 711
AluBI AGCT 6 cut(s) 68, 284, 346, 1006, 1157, 1235
AluI AGCT 6 cut(s) 68, 284, 346, 1006, 1157, 1235
Alw21I GWGCWC 4 cut(s) 317, 348, 681, 853
Alw26I GTCTC 1 cut(s) 120
Alw44I GTGCAC 1 cut(s) 313
AlwI GGATC 1 cut(s) 1019
AoxI GGCC 5 cut(s) 474, 538, 545, 606, 628
ApaLI GTGCAC 1 cut(s) 313
ApeKI GCWGC 9 cut(s) 59, 62, 65, 383, 827, 974, 1000, 1003, 1154
ApoI RAATTY 2 cut(s) 267, 1103
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
Asp700I GAANNNNTTC 1 cut(s) 1200
AspS9I GGNCC 5 cut(s) 538, 545, 607, 618, 629
AsuHPI GGTGA 3 cut(s) 98, 234, 869
AsuNHI GCTAGC 1 cut(s) 55
AvaII GGWCC 1 cut(s) 618
BaeGI GKGCMC 1 cut(s) 317
BalI TGGCCA 1 cut(s) 476
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 348
Bbv12I GWGCWC 4 cut(s) 317, 348, 681, 853
BbvCI CCTCAGC 1 cut(s) 807
BbvI GCAGC 9 cut(s) 71, 74, 77, 370, 839, 961, 1012, 1015, 1166
BccI CCATC 3 cut(s) 137, 142, 689
BciT130I CCWGG 1 cut(s) 326
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 120
BcuI ACTAGT 2 cut(s) 43, 49
BfaI CTAG 6 cut(s) 44, 50, 56, 308, 569, 743
BfuAI ACCTGC 1 cut(s) 811
BglI GCCNNNNNGGC 1 cut(s) 537
BmcAI AGTACT 1 cut(s) 48
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 618
BmgT120I GGNCC 5 cut(s) 538, 545, 607, 618, 629
BmiI GGNNCC 3 cut(s) 27, 208, 619
BmrFI CCNGG 1 cut(s) 326
BmrI ACTGGG 1 cut(s) 626
BmsI GCATC 6 cut(s) 239, 400, 712, 870, 926, 1085
BmtI GCTAGC 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 626
BoxI GACNNNNGTC 1 cut(s) 183
BpmI CTGGAG 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 807
BpuEI CTTGAG 1 cut(s) 997
BsaHI GRCGYC 1 cut(s) 660
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 4 cut(s) 324, 325, 541, 921
BsaWI WCCGGW 2 cut(s) 163, 620
Bsc4I CCNNNNNNNGG 1 cut(s) 615
Bse1I ACTGG 3 cut(s) 406, 601, 632
Bse3DI GCAATG 2 cut(s) 995, 1208
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 4 cut(s) 324, 325, 541, 921
BseGI GGATG 5 cut(s) 230, 371, 885, 917, 1076
BseLI CCNNNNNNNGG 1 cut(s) 615
BseMI GCAATG 2 cut(s) 995, 1208
BseMII CTCAG 2 cut(s) 480, 798
BseNI ACTGG 3 cut(s) 406, 601, 632
BseRI GAGGAG 2 cut(s) 824, 1080
BseSI GKGCMC 1 cut(s) 317
BseXI GCAGC 9 cut(s) 71, 74, 77, 370, 839, 961, 1012, 1015, 1166
Bsh1236I CGCG 1 cut(s) 543
BshFI GGCC 5 cut(s) 476, 540, 547, 608, 630
BshNI GGYRCC 1 cut(s) 206
BsiHKAI GWGCWC 4 cut(s) 317, 348, 681, 853
BsiSI CCGG 2 cut(s) 164, 621
BslFI GGGAC 1 cut(s) 1084
BslI CCNNNNNNNGG 1 cut(s) 615
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 1 cut(s) 1084
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 5 cut(s) 476, 540, 547, 608, 630
Bso31I GGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 4 cut(s) 317, 348, 681, 853
Bsp1407I TGTACA 1 cut(s) 356
Bsp143I GATC 4 cut(s) 33, 91, 465, 1024
Bsp19I CCATGG 1 cut(s) 921
BspACI CCGC 2 cut(s) 541, 543
BspANI GGCC 5 cut(s) 476, 540, 547, 608, 630
BspCNI CTCAG 2 cut(s) 481, 799
BspFNI CGCG 1 cut(s) 543
BspLI GGNNCC 3 cut(s) 27, 208, 619
BspMI ACCTGC 1 cut(s) 811
BspOI GCTAGC 1 cut(s) 59
BspPI GGATC 1 cut(s) 1019
BspQI GCTCTTC 1 cut(s) 648
BspT107I GGYRCC 1 cut(s) 206
BspTNI GGTCTC 1 cut(s) 120
BsrDI GCAATG 2 cut(s) 995, 1208
BsrGI TGTACA 1 cut(s) 356
BsrI ACTGG 3 cut(s) 406, 601, 632
BssECI CCNNGG 4 cut(s) 324, 325, 541, 921
BssMI GATC 4 cut(s) 33, 91, 465, 1024
BssNI GRCGYC 1 cut(s) 660
BssT1I CCWWGG 1 cut(s) 921
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 2 cut(s) 505, 710
Bst6I CTCTTC 1 cut(s) 648
BstACI GRCGYC 1 cut(s) 660
BstAPI GCANNNNNTGC 1 cut(s) 71
BstAUI TGTACA 1 cut(s) 356
BstC8I GCNNGC 6 cut(s) 57, 305, 474, 545, 1152, 1215
BstDEI CTNAG 3 cut(s) 489, 761, 807
BstDSI CCRYGG 2 cut(s) 541, 921
BstF5I GGATG 5 cut(s) 230, 371, 885, 917, 1076
BstFNI CGCG 1 cut(s) 543
BstKTI GATC 4 cut(s) 36, 94, 468, 1027
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 4 cut(s) 33, 91, 465, 1024
BstMWI GCNNNNNNNGC 6 cut(s) 65, 71, 537, 827, 983, 992
BstNI CCWGG 1 cut(s) 326
BstNSI RCATGY 2 cut(s) 303, 307
BstPAI GACNNNNGTC 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 317
BstUI CGCG 1 cut(s) 543
BstV1I GCAGC 9 cut(s) 71, 74, 77, 370, 839, 961, 1012, 1015, 1166
BsuRI GGCC 5 cut(s) 476, 540, 547, 608, 630
BtgI CCRYGG 2 cut(s) 541, 921
BtsCI GGATG 5 cut(s) 230, 371, 885, 917, 1076
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 6 cut(s) 57, 305, 474, 545, 1152, 1215
Cfr13I GGNCC 5 cut(s) 538, 545, 607, 618, 629
Cfr42I CCGCGG 1 cut(s) 544
CseI GACGC 1 cut(s) 668
Csp6I GTAC 4 cut(s) 47, 297, 357, 1127
CspCI CAANNNNNGTGG 2 cut(s) 434, 469
CviAII CATG 9 cut(s) 31, 95, 300, 304, 319, 655, 922, 1136, 1186
CviQI GTAC 4 cut(s) 47, 297, 357, 1127
DdeI CTNAG 3 cut(s) 489, 761, 807
DpnI GATC 4 cut(s) 35, 93, 467, 1026
DpnII GATC 4 cut(s) 33, 91, 465, 1024
EaeI YGGCCR 1 cut(s) 474
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
Ecl136II GAGCTC 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 921
Eco24I GRGCYC 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 618
Eco53kI GAGCTC 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 324
EcoT14I CCWWGG 1 cut(s) 921
EcoT22I ATGCAT 1 cut(s) 305
EcoT38I GRGCYC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 921
FaeI CATG 9 cut(s) 34, 98, 303, 307, 322, 658, 925, 1139, 1189
FalI AAGNNNNNCTT 6 cut(s) 242, 274, 765, 797, 837, 869
FaqI GGGAC 1 cut(s) 1084
FatI CATG 9 cut(s) 30, 94, 299, 303, 318, 654, 921, 1135, 1185
FauI CCCGC 1 cut(s) 536
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 177
FokI GGATG 5 cut(s) 217, 378, 892, 904, 1063
FriOI GRGCYC 1 cut(s) 348
FspBI CTAG 6 cut(s) 44, 50, 56, 308, 569, 743
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 5 cut(s) 476, 540, 547, 608, 630
HapII CCGG 2 cut(s) 164, 621
HgaI GACGC 1 cut(s) 668
Hin1I GRCGYC 1 cut(s) 660
Hin1II CATG 9 cut(s) 34, 98, 303, 307, 322, 658, 925, 1139, 1189
HincII GTYRAC 3 cut(s) 178, 767, 790
HindII GTYRAC 3 cut(s) 178, 767, 790
HindIII AAGCTT 1 cut(s) 1233
HinfI GANTC 3 cut(s) 109, 508, 962
HpaII CCGG 2 cut(s) 164, 621
HphI GGTGA 3 cut(s) 98, 234, 869
Hpy166II GTNNAC 5 cut(s) 178, 315, 359, 767, 790
Hpy188I TCNGA 5 cut(s) 108, 142, 409, 523, 652
Hpy188III TCNNGA 1 cut(s) 719
Hpy8I GTNNAC 5 cut(s) 178, 315, 359, 767, 790
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 2 cut(s) 77, 1139
HpyCH4III ACNGT 2 cut(s) 505, 710
HpyCH4IV ACGT 2 cut(s) 180, 885
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 71, 537, 827, 983, 992
HpyF3I CTNAG 3 cut(s) 489, 761, 807
HpySE526I ACGT 2 cut(s) 180, 885
Hsp92I GRCGYC 1 cut(s) 660
Hsp92II CATG 9 cut(s) 34, 98, 303, 307, 322, 658, 925, 1139, 1189
Ksp22I TGATCA 1 cut(s) 91
KspI CCGCGG 1 cut(s) 544
Kzo9I GATC 4 cut(s) 33, 91, 465, 1024
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 2 cut(s) 281, 824
Lsp1109I GCAGC 9 cut(s) 71, 74, 77, 370, 839, 961, 1012, 1015, 1166
LweI GCATC 6 cut(s) 239, 400, 712, 870, 926, 1085
MaeI CTAG 6 cut(s) 44, 50, 56, 308, 569, 743
MaeII ACGT 2 cut(s) 180, 885
MaeIII GTNAC 2 cut(s) 716, 1051
MalI GATC 4 cut(s) 35, 93, 467, 1026
MboI GATC 4 cut(s) 33, 91, 465, 1024
MboII GAAGA 1 cut(s) 635
MfeI CAATTG 1 cut(s) 451
MhlI GDGCHC 4 cut(s) 317, 348, 681, 853
MlsI TGGCCA 1 cut(s) 476
MluCI AATT 9 cut(s) 267, 395, 451, 612, 754, 987, 1020, 1086, 1103
MluNI TGGCCA 1 cut(s) 476
MlyI GAGTC 2 cut(s) 517, 956
MmeI TCCRAC 1 cut(s) 501
Mox20I TGGCCA 1 cut(s) 476
Mph1103I ATGCAT 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 1200
MscI TGGCCA 1 cut(s) 476
MseI TTAA 2 cut(s) 398, 1118
MslI CAYNNNNRTG 6 cut(s) 35, 276, 364, 711, 734, 1184
Msp20I TGGCCA 1 cut(s) 476
MspA1I CMGCKG 2 cut(s) 543, 1157
MspI CCGG 2 cut(s) 164, 621
MspR9I CCNGG 1 cut(s) 326
MunI CAATTG 1 cut(s) 451
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 543
MwoI GCNNNNNNNGC 6 cut(s) 65, 71, 537, 827, 983, 992
NcoI CCATGG 1 cut(s) 921
NdeII GATC 4 cut(s) 33, 91, 465, 1024
NheI GCTAGC 1 cut(s) 55
NlaIII CATG 9 cut(s) 34, 98, 303, 307, 322, 658, 925, 1139, 1189
NlaIV GGNNCC 3 cut(s) 27, 208, 619
NmuCI GTSAC 2 cut(s) 716, 1051
NsiI ATGCAT 1 cut(s) 305
NspI RCATGY 2 cut(s) 303, 307
OliI CACNNNNGTG 1 cut(s) 711
PaeI GCATGC 1 cut(s) 307
PasI CCCWGGG 1 cut(s) 325
PciSI GCTCTTC 1 cut(s) 648
PctI GAATGC 1 cut(s) 5
PdmI GAANNNNTTC 1 cut(s) 1200
PfeI GAWTC 1 cut(s) 109
PleI GAGTC 2 cut(s) 516, 956
PpsI GAGTC 2 cut(s) 516, 956
PshAI GACNNNNGTC 1 cut(s) 183
PsiI TTATAA 2 cut(s) 375, 462
Psp124BI GAGCTC 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 3 cut(s) 27, 208, 619
PspPI GGNCC 5 cut(s) 538, 545, 607, 618, 629
PvuII CAGCTG 1 cut(s) 1157
RsaI GTAC 4 cut(s) 48, 298, 358, 1128
RsaNI GTAC 4 cut(s) 47, 297, 357, 1127
RseI CAYNNNNRTG 6 cut(s) 35, 276, 364, 711, 734, 1184
SacI GAGCTC 1 cut(s) 348
SacII CCGCGG 1 cut(s) 544
SalI GTCGAC 1 cut(s) 176
SapI GCTCTTC 1 cut(s) 648
SaqAI TTAA 2 cut(s) 398, 1118
Sau3AI GATC 4 cut(s) 33, 91, 465, 1024
Sau96I GGNCC 5 cut(s) 538, 545, 607, 618, 629
ScaI AGTACT 1 cut(s) 48
SchI GAGTC 2 cut(s) 517, 956
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 4 cut(s) 317, 348, 681, 853
SfaNI GCATC 6 cut(s) 239, 400, 712, 870, 926, 1085
Sfr303I CCGCGG 1 cut(s) 544
SgrBI CCGCGG 1 cut(s) 544
SinI GGWCC 1 cut(s) 618
SmiMI CAYNNNNRTG 6 cut(s) 35, 276, 364, 711, 734, 1184
SmlI CTYRAG 1 cut(s) 1012
SmoI CTYRAG 1 cut(s) 1012
SpeI ACTAGT 2 cut(s) 43, 49
SphI GCATGC 1 cut(s) 307
Sse9I AATT 9 cut(s) 267, 395, 451, 612, 754, 987, 1020, 1086, 1103
SsiI CCGC 2 cut(s) 541, 543
SspI AATATT 1 cut(s) 873
SspMI CTAG 6 cut(s) 44, 50, 56, 308, 569, 743
SstI GAGCTC 1 cut(s) 348
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 1 cut(s) 921
TaaI ACNGT 2 cut(s) 505, 710
TaiI ACGT 2 cut(s) 183, 888
TaqI TCGA 2 cut(s) 177, 1063
TaqII GACCGA 2 cut(s) 102, 636
TasI AATT 9 cut(s) 267, 395, 451, 612, 754, 987, 1020, 1086, 1103
TatI WGTACW 2 cut(s) 46, 356
TauI GCSGC 1 cut(s) 543
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 2 cut(s) 398, 1118
Tru9I TTAA 2 cut(s) 398, 1118
TseFI GTSAC 2 cut(s) 716, 1051
TseI GCWGC 9 cut(s) 59, 62, 65, 383, 827, 974, 1000, 1003, 1154
Tsp45I GTSAC 2 cut(s) 716, 1051
TspDTI ATGAA 3 cut(s) 260, 509, 1202
TspGWI ACGGA 1 cut(s) 784
VneI GTGCAC 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 618
XapI RAATTY 2 cut(s) 267, 1103
XceI RCATGY 2 cut(s) 303, 307
XmiI GTMKAC 1 cut(s) 177
XmnI GAANNNNTTC 1 cut(s) 1200
XspI CTAG 6 cut(s) 44, 50, 56, 308, 569, 743
ZrmI AGTACT 1 cut(s) 48
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.