Rh1CG435300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0005513
Physical Location & Seq
Forward (+)
69035195 .. 69035764
570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG435300.1

Sequence Viewer

Length: 477 bp
ATGGCATTCGCAACAGATAGTAGGGTTTCCATGATCGTGCTACTAGTACTAGTGCTAGCAGCAGCAGCTCTTGCTCATAATGAAGGTGATGATCATGCAACGCCTCCGATTCGGTCTCCAAATCCATTGTCGCCATCATCAGATGGTTCGGCTTCTGCTGAACCGGAGGGTTTAGTCGACGTAGGTCTATGTGCCGTTCAATGTGGTGCTAAGTGTTTTGGTCGCAAGGCATCCCTGGTTTCACCTGATAAGTTTCGCTTTCCCAAAATTCATTTTGGTGGAGCTTTTTGTTTTGGTACATGCATGCTAGAGTGCACCATGACCCAGGGGAATGTGAAGCCAGAGCTCTATCATTGTACACTTCGATGTGCTTATAAGTATGCTGCTAAAACAAAATTAACTGGTTCAGATGCTCATAGAGGGAATAGCCATTGGGGTTCTTGCTACAACAAGTGCGAGAAGAACTATCAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

16.89

Weight (kDa)

8.33

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AccI GTMKAC 1 cut(s) 177
AcsI RAATTY 1 cut(s) 267
AfaI GTAC 3 cut(s) 48, 298, 358
AgsI TTSAA 1 cut(s) 200
AhlI ACTAGT 2 cut(s) 43, 49
AjnI CCWGG 2 cut(s) 234, 324
AluBI AGCT 3 cut(s) 68, 284, 346
AluI AGCT 3 cut(s) 68, 284, 346
Alw21I GWGCWC 2 cut(s) 317, 348
Alw26I GTCTC 1 cut(s) 120
Alw44I GTGCAC 1 cut(s) 313
ApaLI GTGCAC 1 cut(s) 313
ApeKI GCWGC 4 cut(s) 59, 62, 65, 383
ApoI RAATTY 1 cut(s) 267
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AsuHPI GGTGA 2 cut(s) 98, 234
AsuNHI GCTAGC 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 317
BanII GRGCYC 1 cut(s) 348
Bbv12I GWGCWC 2 cut(s) 317, 348
BbvI GCAGC 4 cut(s) 71, 74, 77, 370
BccI CCATC 2 cut(s) 137, 142
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 2 cut(s) 236, 326
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 120
BcuI ACTAGT 2 cut(s) 43, 49
BfaI CTAG 4 cut(s) 44, 50, 56, 308
BisI GCNGC 4 cut(s) 60, 63, 66, 384
BlsI GCNGC 4 cut(s) 61, 64, 67, 385
BmcAI AGTACT 1 cut(s) 48
Bme1390I CCNGG 2 cut(s) 236, 326
BmrFI CCNGG 2 cut(s) 236, 326
BmsI GCATC 2 cut(s) 239, 400
BmtI GCTAGC 1 cut(s) 59
BoxI GACNNNNGTC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 234, 324, 325
BsaWI WCCGGW 1 cut(s) 163
Bse1I ACTGG 1 cut(s) 406
BseBI CCWGG 2 cut(s) 236, 326
BseDI CCNNGG 3 cut(s) 234, 324, 325
BseGI GGATG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 406
BseSI GKGCMC 1 cut(s) 317
BseXI GCAGC 4 cut(s) 71, 74, 77, 370
BsiHKAI GWGCWC 2 cut(s) 317, 348
BsiSI CCGG 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 120
BsmI GAATGC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 2 cut(s) 317, 348
Bsp1407I TGTACA 1 cut(s) 356
Bsp143I GATC 2 cut(s) 33, 91
BspOI GCTAGC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 120
BsrGI TGTACA 1 cut(s) 356
BsrI ACTGG 1 cut(s) 406
BssECI CCNNGG 3 cut(s) 234, 324, 325
BssMI GATC 2 cut(s) 33, 91
Bst2UI CCWGG 2 cut(s) 236, 326
BstAPI GCANNNNNTGC 1 cut(s) 71
BstAUI TGTACA 1 cut(s) 356
BstC8I GCNNGC 2 cut(s) 57, 305
BstDEI CTNAG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 230
BstKTI GATC 2 cut(s) 36, 94
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 2 cut(s) 33, 91
BstMWI GCNNNNNNNGC 2 cut(s) 65, 71
BstNI CCWGG 2 cut(s) 236, 326
BstNSI RCATGY 2 cut(s) 303, 307
BstPAI GACNNNNGTC 1 cut(s) 183
BstSCI CCNGG 2 cut(s) 234, 324
BstSLI GKGCMC 1 cut(s) 317
BstV1I GCAGC 4 cut(s) 71, 74, 77, 370
BtsCI GGATG 1 cut(s) 230
Cac8I GCNNGC 2 cut(s) 57, 305
Csp6I GTAC 3 cut(s) 47, 297, 357
CviAII CATG 5 cut(s) 31, 95, 300, 304, 319
CviJI RGCY 6 cut(s) 68, 152, 284, 340, 346, 429
CviKI_1 RGCY 6 cut(s) 68, 152, 284, 340, 346, 429
CviQI GTAC 3 cut(s) 47, 297, 357
DdeI CTNAG 1 cut(s) 210
DpnI GATC 2 cut(s) 35, 93
DpnII GATC 2 cut(s) 33, 91
Ecl136II GAGCTC 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 120
Eco53kI GAGCTC 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 234, 324
EcoT22I ATGCAT 1 cut(s) 305
EcoT38I GRGCYC 1 cut(s) 348
FaeI CATG 5 cut(s) 34, 98, 303, 307, 322
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FatI CATG 5 cut(s) 30, 94, 299, 303, 318
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 4 cut(s) 60, 63, 66, 384
FokI GGATG 1 cut(s) 217
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 4 cut(s) 60, 63, 66, 384
FspBI CTAG 4 cut(s) 44, 50, 56, 308
GluI GCNGC 4 cut(s) 60, 63, 66, 384
HapII CCGG 1 cut(s) 164
Hin1II CATG 5 cut(s) 34, 98, 303, 307, 322
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 1 cut(s) 109
HpaII CCGG 1 cut(s) 164
HphI GGTGA 2 cut(s) 98, 234
Hpy166II GTNNAC 3 cut(s) 178, 315, 359
Hpy188I TCNGA 3 cut(s) 108, 142, 409
Hpy8I GTNNAC 3 cut(s) 178, 315, 359
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 77
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 3 cut(s) 98, 303, 315
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 71
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 5 cut(s) 34, 98, 303, 307, 322
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 2 cut(s) 33, 91
LmnI GCTCC 1 cut(s) 281
LpnPI CCDG 8 cut(s) 177, 221, 248, 258, 311, 338, 354, 387
Lsp1109I GCAGC 4 cut(s) 71, 74, 77, 370
LweI GCATC 2 cut(s) 239, 400
MaeI CTAG 4 cut(s) 44, 50, 56, 308
MaeII ACGT 1 cut(s) 180
MalI GATC 2 cut(s) 35, 93
MboI GATC 2 cut(s) 33, 91
MboII GAAGA 1 cut(s) 472
MhlI GDGCHC 2 cut(s) 317, 348
MluCI AATT 2 cut(s) 267, 395
MnlI CCTC 3 cut(s) 114, 160, 413
Mph1103I ATGCAT 1 cut(s) 305
MseI TTAA 1 cut(s) 398
MslI CAYNNNNRTG 3 cut(s) 35, 276, 364
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 2 cut(s) 236, 326
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 2 cut(s) 236, 326
MwoI GCNNNNNNNGC 2 cut(s) 65, 71
NdeII GATC 2 cut(s) 33, 91
NheI GCTAGC 1 cut(s) 55
NlaIII CATG 5 cut(s) 34, 98, 303, 307, 322
NsiI ATGCAT 1 cut(s) 305
NspI RCATGY 2 cut(s) 303, 307
PaeI GCATGC 1 cut(s) 307
PasI CCCWGGG 1 cut(s) 325
PctI GAATGC 1 cut(s) 5
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 4 cut(s) 61, 64, 67, 385
PshAI GACNNNNGTC 1 cut(s) 183
PsiI TTATAA 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 348
Psp6I CCWGG 2 cut(s) 234, 324
PspGI CCWGG 2 cut(s) 234, 324
RsaI GTAC 3 cut(s) 48, 298, 358
RsaNI GTAC 3 cut(s) 47, 297, 357
RseI CAYNNNNRTG 3 cut(s) 35, 276, 364
SacI GAGCTC 1 cut(s) 348
SalI GTCGAC 1 cut(s) 176
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 4 cut(s) 60, 63, 66, 384
Sau3AI GATC 2 cut(s) 33, 91
ScaI AGTACT 1 cut(s) 48
ScrFI CCNGG 2 cut(s) 236, 326
SduI GDGCHC 2 cut(s) 317, 348
SetI ASST 8 cut(s) 70, 88, 183, 187, 247, 286, 348, 475
SfaNI GCATC 2 cut(s) 239, 400
SmiMI CAYNNNNRTG 3 cut(s) 35, 276, 364
SpeI ACTAGT 2 cut(s) 43, 49
SphI GCATGC 1 cut(s) 307
Sse9I AATT 2 cut(s) 267, 395
SspMI CTAG 4 cut(s) 44, 50, 56, 308
SstI GAGCTC 1 cut(s) 348
StyD4I CCNGG 2 cut(s) 234, 324
TaiI ACGT 1 cut(s) 183
TaqI TCGA 2 cut(s) 177, 364
TaqII GACCGA 1 cut(s) 102
TasI AATT 2 cut(s) 267, 395
TatI WGTACW 2 cut(s) 46, 356
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TseI GCWGC 4 cut(s) 59, 62, 65, 383
TspDTI ATGAA 2 cut(s) 96, 260
VneI GTGCAC 1 cut(s) 313
XapI RAATTY 1 cut(s) 267
XceI RCATGY 2 cut(s) 303, 307
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 4 cut(s) 44, 50, 56, 308
ZrmI AGTACT 1 cut(s) 48
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.