RLG00000027013

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016394
Physical Location & Seq
Reverse (-)
7667976 .. 7672745
4770 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027013

Sequence Viewer

Length: 933 bp
ATGGAGGATTTCAATTTAGAATTGCTCCAAAAGTTCAGGAAATTCAAGACAGAAAAATTCTCAGAGCAGGATCGAATTAATGAAGATCAGGAAAATAAGTTTAAGAACTATATCTCTGTGTCAGAAGAGCTCAGCCAGTACTTGCATTCCTCGATTTGGGAGTTGAAAATGCAAGTCAAGGACTTAAGAAAAGAACTGGTTTCTCTTAGAGCCACGAAAGATGACCAATCTACGGAATACCAGAAGCACTTAATGGAAGAGGAGCAGAAGGTGGAGGTGTTAAAGCTGCGTTTGAAAATCGAAAAGGAGAGGAACAGATCACAGGCAAATGAAGTGGCTTCTCTTAGGGCTGCAAAGGATGAGGAAATTATGGAATACCGGAAGCTTTTGATGGAAGAGAAGCAGAAGAACAAAGAACTTTCTGATGAAGTAGAGAGACTTCAACAGCTGCAAGCGGAGCTAATTTCTAGCAGATTGAAGGATACAAAAGATGCACAACTGTGTATTACTACTGCTGGTGGTCATATACATTTGCAGATTTCGGCAACGATCGCGAAGGATAGGGGCGATGGTCCGCTTTGCTGGGGTGGTGCCCTTGGGGAGCCCTTAGCACAGATGGTCTTCTGGGCCTCTTTCTGGAGTGTGGACTTTAAGCAAGGACTCTTCGATCGCAGTTGGACGGAGGCTGTGGATGATGGAGGCTGGTCTTGGTGGGTGGCTTGGGCCTCCAGGGGGGTTTGCTTTTTTGGGCTAGGGCTCCTGGGGGGCTGGTTTGATTTGTGTTGGTGGGAGTGGTTCTCGGCTTATCAGCAAAGTGGCATTTTGTTTGTTCTATGTTTGGTCATGATTCCTCTTTTCAAGGTGGAGGCTGTGACTATGCCGTTTGTGGAATCTGCTACTGCAGAGGAATATGACAATGGTCAAGTTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

311

Amino Acids

36.15

Weight (kDa)

4.97

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016394)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24870 FvH4_7g24870 FvH4_7g24870 FvH4_7g24870
rosa_chinensis RchiOBHm_Chr1g0370751
rosa_laevigata RLG00000027013
rosa_multiflora Rmu_sc0004550.1_g000018
rosa_roxburghii Rroxscaffold_4G00286270
rosa_rugosa Rorug01G0359300 Rorug07G0063000
rosa_samantha Rh1AG368500 Rh1BG332100 Rh1CG346000 Rh1DG363500
rosa_wichuraiana Rw1G032440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 590
AccII CGCG 1 cut(s) 554
AciI CCGC 2 cut(s) 455, 575
AclWI GGATC 1 cut(s) 78
AcsI RAATTY 2 cut(s) 41, 56
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 4 cut(s) 156, 232, 636, 732
AflII CTTAAG 1 cut(s) 184
AgsI TTSAA 8 cut(s) 13, 46, 166, 295, 443, 478, 859, 929
AjnI CCWGG 2 cut(s) 728, 759
AleI CACNNNNGTG 1 cut(s) 499
AluBI AGCT 5 cut(s) 130, 286, 385, 448, 460
AluI AGCT 5 cut(s) 130, 286, 385, 448, 460
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 430
AlwI GGATC 1 cut(s) 78
AoxI GGCC 2 cut(s) 627, 723
ApeKI GCWGC 3 cut(s) 286, 350, 448
ApoI RAATTY 2 cut(s) 41, 56
AseI ATTAAT 1 cut(s) 78
AspS9I GGNCC 3 cut(s) 572, 627, 723
AvaII GGWCC 1 cut(s) 572
BaeGI GKGCMC 1 cut(s) 595
BanI GGYRCC 1 cut(s) 590
BanII GRGCYC 3 cut(s) 132, 606, 759
BarI GAAGNNNNNNTAC 2 cut(s) 423, 455
BbsI GAAGAC 1 cut(s) 613
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 3 cut(s) 273, 337, 435
BccI CCATC 4 cut(s) 385, 563, 610, 689
BceAI ACGGC 1 cut(s) 865
BciT130I CCWGG 2 cut(s) 730, 761
BciVI GTATCC 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 430
BfaI CTAG 2 cut(s) 468, 752
BfmI CTRYAG 1 cut(s) 900
BfrI CTTAAG 1 cut(s) 184
BfuI GTATCC 1 cut(s) 475
BisI GCNGC 3 cut(s) 287, 351, 449
BlpI GCTNAGC 1 cut(s) 131
BlsI GCNGC 3 cut(s) 288, 352, 450
BmcAI AGTACT 1 cut(s) 140
Bme1390I CCNGG 2 cut(s) 730, 761
Bme18I GGWCC 1 cut(s) 572
BmgT120I GGNCC 3 cut(s) 572, 627, 723
BmiI GGNNCC 3 cut(s) 592, 603, 758
BmrFI CCNGG 2 cut(s) 730, 761
BmsI GCATC 1 cut(s) 481
BoxI GACNNNNGTC 1 cut(s) 918
BpiI GAAGAC 1 cut(s) 613
BplI GAGNNNNNCTC 2 cut(s) 782, 814
BpmI CTGGAG 2 cut(s) 658, 712
Bpu10I CCTNAGC 1 cut(s) 607
Bpu1102I GCTNAGC 1 cut(s) 131
BsaJI CCNNGG 3 cut(s) 595, 729, 760
BsaWI WCCGGW 1 cut(s) 378
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 232, 636, 732
Bse1I ACTGG 2 cut(s) 136, 201
BseBI CCWGG 2 cut(s) 730, 761
BseDI CCNNGG 3 cut(s) 595, 729, 760
BseGI GGATG 2 cut(s) 364, 697
BseLI CCNNNNNNNGG 4 cut(s) 156, 232, 636, 732
BseMII CTCAG 2 cut(s) 75, 145
BseNI ACTGG 2 cut(s) 136, 201
BseRI GAGGAG 1 cut(s) 275
BseSI GKGCMC 1 cut(s) 595
BseXI GCAGC 3 cut(s) 273, 337, 435
BseYI CCCAGC 1 cut(s) 582
Bsh1236I CGCG 1 cut(s) 554
Bsh1285I CGRYCG 2 cut(s) 552, 670
BshFI GGCC 2 cut(s) 629, 725
BshNI GGYRCC 1 cut(s) 590
BsiEI CGRYCG 2 cut(s) 552, 670
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 1 cut(s) 379
BslI CCNNNNNNNGG 4 cut(s) 156, 232, 636, 732
BsmAI GTCTC 1 cut(s) 430
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 2 cut(s) 629, 725
Bsp1286I GDGCHC 4 cut(s) 132, 595, 606, 759
Bsp143I GATC 5 cut(s) 70, 85, 317, 549, 667
Bsp1720I GCTNAGC 1 cut(s) 131
Bsp68I TCGCGA 1 cut(s) 554
BspACI CCGC 2 cut(s) 455, 575
BspANI GGCC 2 cut(s) 629, 725
BspCNI CTCAG 2 cut(s) 74, 144
BspFNI CGCG 1 cut(s) 554
BspHI TCATGA 1 cut(s) 843
BspLI GGNNCC 3 cut(s) 592, 603, 758
BspMAI CTGCAG 1 cut(s) 904
BspPI GGATC 1 cut(s) 78
BspQI GCTCTTC 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 590
BspTI CTTAAG 1 cut(s) 184
BsrI ACTGG 2 cut(s) 136, 201
BssECI CCNNGG 3 cut(s) 595, 729, 760
BssMI GATC 5 cut(s) 70, 85, 317, 549, 667
BssT1I CCWWGG 1 cut(s) 595
Bst2UI CCWGG 2 cut(s) 730, 761
Bst4CI ACNGT 1 cut(s) 501
Bst6I CTCTTC 4 cut(s) 120, 252, 390, 668
BstAFI CTTAAG 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 5 cut(s) 61, 131, 206, 344, 607
BstF5I GGATG 2 cut(s) 364, 697
BstFNI CGCG 1 cut(s) 554
BstKTI GATC 5 cut(s) 73, 88, 320, 552, 670
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 5 cut(s) 70, 85, 317, 549, 667
BstMCI CGRYCG 2 cut(s) 552, 670
BstMWI GCNNNNNNNGC 2 cut(s) 457, 551
BstNI CCWGG 2 cut(s) 730, 761
BstPAI GACNNNNGTC 1 cut(s) 918
BstSCI CCNGG 2 cut(s) 728, 759
BstSFI CTRYAG 1 cut(s) 900
BstSLI GKGCMC 1 cut(s) 595
BstUI CGCG 1 cut(s) 554
BstV1I GCAGC 3 cut(s) 273, 337, 435
BstV2I GAAGAC 1 cut(s) 613
BsuI GTATCC 1 cut(s) 475
BsuRI GGCC 2 cut(s) 629, 725
BtgZI GCGATG 1 cut(s) 582
BtsCI GGATG 2 cut(s) 364, 697
BtuMI TCGCGA 1 cut(s) 554
Cac8I GCNNGC 1 cut(s) 453
CciI TCATGA 1 cut(s) 843
Cfr13I GGNCC 3 cut(s) 572, 627, 723
Csp6I GTAC 1 cut(s) 139
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 1 cut(s) 844
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 5 cut(s) 61, 131, 206, 344, 607
DpnI GATC 5 cut(s) 72, 87, 319, 551, 669
DpnII GATC 5 cut(s) 70, 85, 317, 549, 667
Eam1104I CTCTTC 4 cut(s) 120, 252, 390, 668
EarI CTCTTC 4 cut(s) 120, 252, 390, 668
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 595
Eco24I GRGCYC 3 cut(s) 132, 606, 759
Eco47I GGWCC 1 cut(s) 572
Eco53kI GAGCTC 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 130
EcoRII CCWGG 2 cut(s) 728, 759
EcoT14I CCWWGG 1 cut(s) 595
EcoT38I GRGCYC 3 cut(s) 132, 606, 759
ErhI CCWWGG 1 cut(s) 595
FaeI CATG 1 cut(s) 847
FaiI YATR 8 cut(s) 111, 371, 525, 527, 835, 845, 878, 912
FatI CATG 1 cut(s) 843
Fnu4HI GCNGC 3 cut(s) 287, 351, 449
FokI GGATG 2 cut(s) 371, 704
FriOI GRGCYC 3 cut(s) 132, 606, 759
Fsp4HI GCNGC 3 cut(s) 287, 351, 449
FspBI CTAG 2 cut(s) 468, 752
GluI GCNGC 3 cut(s) 287, 351, 449
GsaI CCCAGC 1 cut(s) 586
GsuI CTGGAG 2 cut(s) 658, 712
HaeIII GGCC 2 cut(s) 629, 725
HapII CCGG 1 cut(s) 379
Hin1II CATG 1 cut(s) 847
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 3 cut(s) 660, 847, 890
HpaII CCGG 1 cut(s) 379
Hpy166II GTNNAC 1 cut(s) 646
Hpy188I TCNGA 3 cut(s) 64, 124, 424
Hpy188III TCNNGA 6 cut(s) 37, 46, 89, 553, 637, 844
Hpy8I GTNNAC 1 cut(s) 646
HpyAV CCTTC 3 cut(s) 262, 472, 550
HpyCH4III ACNGT 1 cut(s) 501
HpyCH4V TGCA 7 cut(s) 145, 172, 353, 451, 494, 535, 902
HpyF10VI GCNNNNNNNGC 2 cut(s) 457, 551
HpyF3I CTNAG 5 cut(s) 61, 131, 206, 344, 607
Hsp92II CATG 1 cut(s) 847
Kzo9I GATC 5 cut(s) 70, 85, 317, 549, 667
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 5 cut(s) 30, 262, 457, 601, 762
Lsp1109I GCAGC 3 cut(s) 273, 337, 435
LweI GCATC 1 cut(s) 481
MaeI CTAG 2 cut(s) 468, 752
MaeIII GTNAC 1 cut(s) 871
MalI GATC 5 cut(s) 72, 87, 319, 551, 669
MboI GATC 5 cut(s) 70, 85, 317, 549, 667
MboII GAAGA 7 cut(s) 95, 137, 269, 407, 418, 613, 655
MhlI GDGCHC 4 cut(s) 132, 595, 606, 759
MluCI AATT 7 cut(s) 13, 20, 41, 56, 75, 366, 462
MlyI GAGTC 1 cut(s) 654
MmeI TCCRAC 1 cut(s) 656
MseI TTAA 6 cut(s) 78, 102, 185, 251, 281, 651
MslI CAYNNNNRTG 1 cut(s) 499
MspA1I CMGCKG 1 cut(s) 448
MspCI CTTAAG 1 cut(s) 184
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 2 cut(s) 730, 761
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 2 cut(s) 730, 761
MvnI CGCG 1 cut(s) 554
MwoI GCNNNNNNNGC 2 cut(s) 457, 551
NdeII GATC 5 cut(s) 70, 85, 317, 549, 667
NlaIII CATG 1 cut(s) 847
NlaIV GGNNCC 3 cut(s) 592, 603, 758
NmeAIII GCCGAG 1 cut(s) 779
NmuCI GTSAC 1 cut(s) 871
NruI TCGCGA 1 cut(s) 554
OliI CACNNNNGTG 1 cut(s) 499
PagI TCATGA 1 cut(s) 843
PciSI GCTCTTC 1 cut(s) 120
PctI GAATGC 1 cut(s) 145
PfeI GAWTC 2 cut(s) 847, 890
PkrI GCNGC 3 cut(s) 288, 352, 450
Ple19I CGATCG 2 cut(s) 552, 670
PleI GAGTC 1 cut(s) 654
PpsI GAGTC 1 cut(s) 654
PshAI GACNNNNGTC 1 cut(s) 918
PshBI ATTAAT 1 cut(s) 78
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 2 cut(s) 728, 759
PspFI CCCAGC 1 cut(s) 582
PspGI CCWGG 2 cut(s) 728, 759
PspN4I GGNNCC 3 cut(s) 592, 603, 758
PspPI GGNCC 3 cut(s) 572, 627, 723
PstI CTGCAG 1 cut(s) 904
PvuI CGATCG 2 cut(s) 552, 670
PvuII CAGCTG 1 cut(s) 448
RruI TCGCGA 1 cut(s) 554
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 499
SacI GAGCTC 1 cut(s) 132
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 6 cut(s) 78, 102, 185, 251, 281, 651
SatI GCNGC 3 cut(s) 287, 351, 449
Sau3AI GATC 5 cut(s) 70, 85, 317, 549, 667
Sau96I GGNCC 3 cut(s) 572, 627, 723
ScaI AGTACT 1 cut(s) 140
SchI GAGTC 1 cut(s) 654
ScrFI CCNGG 2 cut(s) 730, 761
SduI GDGCHC 4 cut(s) 132, 595, 606, 759
SetI ASST 8 cut(s) 132, 273, 279, 288, 387, 450, 462, 864
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 1 cut(s) 900
SinI GGWCC 1 cut(s) 572
SmiMI CAYNNNNRTG 1 cut(s) 499
SmlI CTYRAG 1 cut(s) 184
SmoI CTYRAG 1 cut(s) 184
Sse9I AATT 7 cut(s) 13, 20, 41, 56, 75, 366, 462
SsiI CCGC 2 cut(s) 455, 575
SspMI CTAG 2 cut(s) 468, 752
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 2 cut(s) 728, 759
StyI CCWWGG 1 cut(s) 595
TaaI ACNGT 1 cut(s) 501
TaqI TCGA 4 cut(s) 73, 152, 300, 666
TasI AATT 7 cut(s) 13, 20, 41, 56, 75, 366, 462
TatI WGTACW 1 cut(s) 138
TfiI GAWTC 2 cut(s) 847, 890
Tru1I TTAA 6 cut(s) 78, 102, 185, 251, 281, 651
Tru9I TTAA 6 cut(s) 78, 102, 185, 251, 281, 651
TseFI GTSAC 1 cut(s) 871
TseI GCWGC 3 cut(s) 286, 350, 448
Tsp45I GTSAC 1 cut(s) 871
TspDTI ATGAA 3 cut(s) 96, 345, 441
TspGWI ACGGA 2 cut(s) 248, 695
Vha464I CTTAAG 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 572
VspI ATTAAT 1 cut(s) 78
XapI RAATTY 2 cut(s) 41, 56
XspI CTAG 2 cut(s) 468, 752
ZrmI AGTACT 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.