Rorug07G0063000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0016394
Physical Location & Seq
Reverse (-)
4771568 .. 4775367
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0063000.1

Sequence Viewer

Length: 1287 bp
ATGCGCTGCGACGCTGCTTCCTCTTGGCTCTGCGGCCGCCACGTCACCGTCGCAACCGCCATGCCGAACGCCGGAACCTCCTTCTTCTTCCTCTGCAGACCCGCCGTCTCCTGCTGCACCAACACTCCCAACCCACTCAAGCGGCGATACTCCAGTAGTAGCCATGCTCCGGACGTTGTCCGCCGTTGGATTCAATCCGATCATCCGCTAGCTTCTCAGGATAGGTTCACGGTTGCTTCGTACAATATTTTGGGGGATAGAAATGCTTTTCGGCATGGAGACCTGTACGGGAATGTGCCTTCTATGTATCTGAAATGGGACCGCCGGAAAAGGGTCATCTGCGATGAACTTGCTGCCTGGAATTCTGATATAGTTTGCTTGCAAGAGGTGGACAAGTATTTGGAGCTCGCGGATATTTTGGCAAAACTGGGATATGCTGGTTCTTATAAGAGACGCACAGGAGATACCGTTGATGGTTGCGCTACTTTCTGGAAATCTCATAATTTTCGGTTGTTAGAGGAAGAGAGCATTGAATTCAAGGGATATGGACTTCGTGACAATGTTGCTCAACTCTCGGTTTTTGAGATGCATAAAGACAAATCAAGAAAGTTAGTGATCGGTAACATCCATGTGCTTTACAGGCCAAGCCGAGGAGAAGTTAAATTGGGTCAGATTCGTTTTCTCATATCAAGGGCGCAGATCCTCTCGGAGAAATGGGGTAATGCCCCTGTTGTCCTTTGTGGTGATTTCAATAGCACTCCTCAGAGTGCAATATACAAGTTCTTGTCAACATCTGAGCTAAATATCATGTCATATGACAGAACAGAGTTATCTGGGCAGAGGAGCTGTCATCCTGCTCAAGTCTTAGGTGTCAAACAACGAATTAGCAGTCCATTGAGCTTGATAGATGGATTGTTGAAACATAACTGGACAGATGAAGAGGTTAGAGTTGCAACTGGAAATTCTGGCAGCCATTCAGTTGTGCATTCCTTGAAGCTTAACAGTGCTTATGCTACGGTTCAGGGTTCTACAGCAACACGGGGATCCAGTGGTGAGCCTTTAGCTACTTCTTACCACTCAAAGTTTCTTGGAACCGTTGACTACTTATGGTACTCGGATGGTCTTGTGCCGACAGGAGTTATGGATACAGTTCCACTTGACACTCTCCAGAGGATTGGTAGCCTCCCTTGCAAGAAAGTGGGCAGCGATCACCTGGCGTTAGTTTCTGAGTTTGCCTTCACACCAGGCACGAAGGAAGGCAACAACACAACTGCATCAACAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

428

Amino Acids

47.45

Weight (kDa)

8.86

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 79 - 404 9.2e-19 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016394)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24870 FvH4_7g24870 FvH4_7g24870 FvH4_7g24870
rosa_chinensis RchiOBHm_Chr1g0370751
rosa_laevigata RLG00000027013
rosa_multiflora Rmu_sc0004550.1_g000018
rosa_roxburghii Rroxscaffold_4G00286270
rosa_rugosa Rorug01G0359300 Rorug07G0063000
rosa_samantha Rh1AG368500 Rh1BG332100 Rh1CG346000 Rh1DG363500
rosa_wichuraiana Rw1G032440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
AccII CGCG 1 cut(s) 410
AccIII TCCGGA 1 cut(s) 169
AciI CCGC 9 cut(s) 33, 37, 57, 102, 142, 181, 206, 322, 410
AclWI GGATC 3 cut(s) 694, 1038, 1051
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 3 cut(s) 361, 533, 961
AfaI GTAC 3 cut(s) 242, 287, 1112
AfiI CCNNNNNNNGG 4 cut(s) 71, 169, 331, 650
AgsI TTSAA 6 cut(s) 194, 533, 538, 751, 919, 994
AhdI GACNNNNNGTC 1 cut(s) 104
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 3 cut(s) 356, 1212, 1243
AluBI AGCT 7 cut(s) 212, 406, 799, 846, 900, 997, 1064
AluI AGCT 7 cut(s) 212, 406, 799, 846, 900, 997, 1064
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 3 cut(s) 112, 273, 445
AlwI GGATC 3 cut(s) 694, 1038, 1051
Aor13HI TCCGGA 1 cut(s) 169
AoxI GGCC 2 cut(s) 34, 641
ApeKI GCWGC 6 cut(s) 6, 14, 114, 353, 969, 1203
ApoI RAATTY 3 cut(s) 361, 533, 961
ArsI GACNNNNNNTTYG 2 cut(s) 874, 906
AspLEI GCGC 3 cut(s) 6, 482, 697
AspS9I GGNCC 1 cut(s) 319
AsuHPI GGTGA 4 cut(s) 37, 755, 1064, 1202
AsuNHI GCTAGC 1 cut(s) 208
AvaII GGWCC 1 cut(s) 319
BaeI ACNNNNGTAYC 2 cut(s) 139, 172
BamHI GGATCC 1 cut(s) 1043
BanII GRGCYC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 4 cut(s) 101, 340, 981, 1215
BccI CCATC 3 cut(s) 467, 902, 1112
BceAI ACGGC 2 cut(s) 89, 168
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 3 cut(s) 358, 1214, 1245
BciVI GTATCC 1 cut(s) 1138
BcoDI GTCTC 3 cut(s) 112, 273, 445
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 2 cut(s) 94, 1029
BfuI GTATCC 1 cut(s) 1138
BisI GCNGC 9 cut(s) 7, 15, 34, 37, 115, 143, 354, 970, 1204
BlsI GCNGC 9 cut(s) 8, 16, 35, 38, 116, 144, 355, 971, 1205
Bme1390I CCNGG 3 cut(s) 358, 1214, 1245
Bme18I GGWCC 1 cut(s) 319
BmeRI GACNNNNNGTC 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 4 cut(s) 76, 320, 1045, 1093
BmrFI CCNGG 3 cut(s) 358, 1214, 1245
BmrI ACTGGG 1 cut(s) 437
BmsI GCATC 2 cut(s) 576, 1283
BmtI GCTAGC 1 cut(s) 212
BmuI ACTGGG 1 cut(s) 437
BpmI CTGGAG 2 cut(s) 136, 1151
BpuEI CTTGAG 2 cut(s) 122, 843
BsaI GGTCTC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 649
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 453, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 169, 331, 650
Bse1I ACTGG 5 cut(s) 153, 432, 932, 961, 1047
BseAI TCCGGA 1 cut(s) 169
BseBI CCWGG 3 cut(s) 358, 1214, 1245
BseDI CCNNGG 1 cut(s) 649
BseGI GGATG 4 cut(s) 202, 624, 850, 1123
BseLI CCNNNNNNNGG 4 cut(s) 71, 169, 331, 650
BseMII CTCAG 4 cut(s) 230, 776, 786, 1218
BseNI ACTGG 5 cut(s) 153, 432, 932, 961, 1047
BseRI GAGGAG 3 cut(s) 666, 750, 856
BseX3I CGGCCG 1 cut(s) 34
BseXI GCAGC 4 cut(s) 101, 340, 981, 1215
BsgI GTGCAG 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 410
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 36, 643
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 3 cut(s) 72, 170, 325
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 4 cut(s) 71, 169, 331, 650
BsmAI GTCTC 3 cut(s) 112, 273, 445
BsmBI CGTCTC 2 cut(s) 112, 445
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 1 cut(s) 985
BsnI GGCC 2 cut(s) 36, 643
Bso31I GGTCTC 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 408
Bsp13I TCCGGA 1 cut(s) 169
Bsp143I GATC 5 cut(s) 199, 615, 699, 1043, 1207
BspACI CCGC 9 cut(s) 33, 37, 57, 102, 142, 181, 206, 322, 410
BspANI GGCC 2 cut(s) 36, 643
BspCNI CTCAG 4 cut(s) 229, 775, 787, 1219
BspEI TCCGGA 1 cut(s) 169
BspFNI CGCG 1 cut(s) 410
BspLI GGNNCC 4 cut(s) 76, 320, 1045, 1093
BspMAI CTGCAG 1 cut(s) 98
BspOI GCTAGC 1 cut(s) 212
BspPI GGATC 3 cut(s) 694, 1038, 1051
BspTNI GGTCTC 1 cut(s) 273
BsrI ACTGG 5 cut(s) 153, 432, 932, 961, 1047
BssECI CCNNGG 1 cut(s) 649
BssMI GATC 5 cut(s) 199, 615, 699, 1043, 1207
Bst2UI CCWGG 3 cut(s) 358, 1214, 1245
Bst4CI ACNGT 7 cut(s) 49, 232, 469, 1004, 1018, 1096, 1150
Bst6I CTCTTC 2 cut(s) 516, 933
BstC8I GCNNGC 3 cut(s) 210, 380, 408
BstDEI CTNAG 5 cut(s) 216, 762, 795, 865, 1227
BstF5I GGATG 4 cut(s) 202, 624, 850, 1123
BstFNI CGCG 1 cut(s) 410
BstHHI GCGC 3 cut(s) 6, 482, 697
BstKTI GATC 5 cut(s) 202, 618, 702, 1046, 1210
BstMAI GTCTC 3 cut(s) 112, 273, 445
BstMBI GATC 5 cut(s) 199, 615, 699, 1043, 1207
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 2 cut(s) 640, 1188
BstNI CCWGG 3 cut(s) 358, 1214, 1245
BstSCI CCNGG 3 cut(s) 356, 1212, 1243
BstSFI CTRYAG 2 cut(s) 94, 1029
BstUI CGCG 1 cut(s) 410
BstV1I GCAGC 4 cut(s) 101, 340, 981, 1215
BstX2I RGATCY 2 cut(s) 699, 1043
BstXI CCANNNNNNTGG 1 cut(s) 1175
BstYI RGATCY 2 cut(s) 699, 1043
BstZI CGGCCG 1 cut(s) 34
BsuI GTATCC 1 cut(s) 1138
BsuRI GGCC 2 cut(s) 36, 643
BtgZI GCGATG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 4 cut(s) 202, 624, 850, 1123
BtsIMutI CAGTG 2 cut(s) 1009, 1054
Cac8I GCNNGC 3 cut(s) 210, 380, 408
CciNI GCGGCCGC 1 cut(s) 34
CfoI GCGC 3 cut(s) 6, 482, 697
Cfr13I GGNCC 1 cut(s) 319
CseI GACGC 2 cut(s) 20, 462
Csp6I GTAC 3 cut(s) 241, 286, 1111
CviAII CATG 5 cut(s) 61, 164, 275, 629, 808
CviQI GTAC 3 cut(s) 241, 286, 1111
DdeI CTNAG 5 cut(s) 216, 762, 795, 865, 1227
DpnI GATC 5 cut(s) 201, 617, 701, 1045, 1209
DpnII GATC 5 cut(s) 199, 615, 699, 1043, 1207
DriI GACNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 1 cut(s) 34
EagI CGGCCG 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 516, 933
Eam1105I GACNNNNNGTC 1 cut(s) 104
EarI CTCTTC 2 cut(s) 516, 933
EciI GGCGGA 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 406
EclXI CGGCCG 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 408
Eco31I GGTCTC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 319
Eco52I CGGCCG 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 406
EcoRI GAATTC 2 cut(s) 361, 533
EcoRII CCWGG 3 cut(s) 356, 1212, 1243
EcoT22I ATGCAT 1 cut(s) 591
EcoT38I GRGCYC 1 cut(s) 408
Esp3I CGTCTC 2 cut(s) 112, 445
FaeI CATG 5 cut(s) 64, 167, 278, 632, 811
FaqI GGGAC 1 cut(s) 332
FatI CATG 5 cut(s) 60, 163, 274, 628, 807
FauI CCCGC 1 cut(s) 109
FauNDI CATATG 1 cut(s) 814
Fnu4HI GCNGC 9 cut(s) 7, 15, 34, 37, 115, 143, 354, 970, 1204
FokI GGATG 4 cut(s) 189, 611, 837, 1130
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 9 cut(s) 7, 15, 34, 37, 115, 143, 354, 970, 1204
FspBI CTAG 1 cut(s) 209
GlaI GCGC 3 cut(s) 5, 481, 696
GluI GCNGC 9 cut(s) 7, 15, 34, 37, 115, 143, 354, 970, 1204
GsuI CTGGAG 2 cut(s) 136, 1151
HaeIII GGCC 2 cut(s) 36, 643
HapII CCGG 3 cut(s) 72, 170, 325
HgaI GACGC 2 cut(s) 20, 462
HhaI GCGC 3 cut(s) 6, 482, 697
Hin1II CATG 5 cut(s) 64, 167, 278, 632, 811
Hin6I GCGC 3 cut(s) 4, 480, 695
HinP1I GCGC 3 cut(s) 4, 480, 695
HincII GTYRAC 2 cut(s) 789, 1099
HindII GTYRAC 2 cut(s) 789, 1099
HindIII AAGCTT 1 cut(s) 995
HinfI GANTC 2 cut(s) 190, 673
HpaII CCGG 3 cut(s) 72, 170, 325
HphI GGTGA 4 cut(s) 37, 755, 1064, 1202
Hpy166II GTNNAC 4 cut(s) 228, 391, 789, 1099
Hpy188III TCNNGA 6 cut(s) 170, 218, 490, 554, 603, 1168
Hpy8I GTNNAC 4 cut(s) 228, 391, 789, 1099
Hpy99I CGWCG 2 cut(s) 14, 53
HpyAV CCTTC 5 cut(s) 91, 309, 1246, 1246, 1250
HpyCH4III ACNGT 7 cut(s) 49, 232, 469, 1004, 1018, 1096, 1150
HpyCH4IV ACGT 2 cut(s) 42, 174
HpyCH4V TGCA 9 cut(s) 96, 117, 382, 589, 770, 953, 985, 1191, 1274
HpyF10VI GCNNNNNNNGC 2 cut(s) 640, 1188
HpyF3I CTNAG 5 cut(s) 216, 762, 795, 865, 1227
HpySE526I ACGT 2 cut(s) 42, 174
Hsp92II CATG 5 cut(s) 64, 167, 278, 632, 811
HspAI GCGC 3 cut(s) 4, 480, 695
Kpn2I TCCGGA 1 cut(s) 169
Kzo9I GATC 5 cut(s) 199, 615, 699, 1043, 1207
LmnI GCTCC 3 cut(s) 172, 403, 843
Lsp1109I GCAGC 4 cut(s) 101, 340, 981, 1215
LweI GCATC 2 cut(s) 576, 1283
MaeI CTAG 1 cut(s) 209
MaeII ACGT 2 cut(s) 42, 174
MaeIII GTNAC 3 cut(s) 43, 554, 620
MalI GATC 5 cut(s) 201, 617, 701, 1045, 1209
MboI GATC 5 cut(s) 199, 615, 699, 1043, 1207
MboII GAAGA 4 cut(s) 76, 79, 533, 950
MflI RGATCY 2 cut(s) 699, 1043
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 6 cut(s) 361, 502, 533, 662, 882, 961
MmeI TCCRAC 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 591
MroI TCCGGA 1 cut(s) 169
MseI TTAA 2 cut(s) 660, 999
MslI CAYNNNNRTG 1 cut(s) 629
MspI CCGG 3 cut(s) 72, 170, 325
MspR9I CCNGG 3 cut(s) 358, 1214, 1245
Mva1269I GAATGC 1 cut(s) 985
MvaI CCWGG 3 cut(s) 358, 1214, 1245
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 2 cut(s) 640, 1188
NdeI CATATG 1 cut(s) 814
NdeII GATC 5 cut(s) 199, 615, 699, 1043, 1207
NheI GCTAGC 1 cut(s) 208
NlaIII CATG 5 cut(s) 64, 167, 278, 632, 811
NlaIV GGNNCC 4 cut(s) 76, 320, 1045, 1093
NmeAIII GCCGAG 1 cut(s) 674
NmuCI GTSAC 2 cut(s) 43, 554
NotI GCGGCCGC 1 cut(s) 34
NsiI ATGCAT 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PctI GAATGC 1 cut(s) 985
PfeI GAWTC 2 cut(s) 190, 673
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 9 cut(s) 8, 16, 35, 38, 116, 144, 355, 971, 1205
PsiI TTATAA 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 408
Psp6I CCWGG 3 cut(s) 356, 1212, 1243
PspGI CCWGG 3 cut(s) 356, 1212, 1243
PspN4I GGNNCC 4 cut(s) 76, 320, 1045, 1093
PspPI GGNCC 1 cut(s) 319
PstI CTGCAG 1 cut(s) 98
PsuI RGATCY 2 cut(s) 699, 1043
PsyI GACNNNGTC 1 cut(s) 176
RsaI GTAC 3 cut(s) 242, 287, 1112
RsaNI GTAC 3 cut(s) 241, 286, 1111
RseI CAYNNNNRTG 1 cut(s) 629
SacI GAGCTC 1 cut(s) 408
SaqAI TTAA 2 cut(s) 660, 999
SatI GCNGC 9 cut(s) 7, 15, 34, 37, 115, 143, 354, 970, 1204
Sau3AI GATC 5 cut(s) 199, 615, 699, 1043, 1207
Sau96I GGNCC 1 cut(s) 319
ScrFI CCNGG 3 cut(s) 358, 1214, 1245
SduI GDGCHC 1 cut(s) 408
SfaNI GCATC 2 cut(s) 576, 1283
SfcI CTRYAG 2 cut(s) 94, 1029
SinI GGWCC 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 629
SmlI CTYRAG 2 cut(s) 137, 858
SmoI CTYRAG 2 cut(s) 137, 858
Sse9I AATT 6 cut(s) 361, 502, 533, 662, 882, 961
SsiI CCGC 9 cut(s) 33, 37, 57, 102, 142, 181, 206, 322, 410
SspI AATATT 1 cut(s) 247
SspMI CTAG 1 cut(s) 209
SstI GAGCTC 1 cut(s) 408
StyD4I CCNGG 3 cut(s) 356, 1212, 1243
TaaI ACNGT 7 cut(s) 49, 232, 469, 1004, 1018, 1096, 1150
TaiI ACGT 2 cut(s) 45, 177
TasI AATT 6 cut(s) 361, 502, 533, 662, 882, 961
TauI GCSGC 3 cut(s) 36, 39, 145
TfiI GAWTC 2 cut(s) 190, 673
Tru1I TTAA 2 cut(s) 660, 999
Tru9I TTAA 2 cut(s) 660, 999
TscAI CASTG 2 cut(s) 1009, 1054
TseFI GTSAC 2 cut(s) 43, 554
TseI GCWGC 6 cut(s) 6, 14, 114, 353, 969, 1203
Tsp45I GTSAC 2 cut(s) 43, 554
TspDTI ATGAA 2 cut(s) 360, 951
TspRI CASTG 2 cut(s) 1009, 1054
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 3 cut(s) 361, 533, 961
XspI CTAG 1 cut(s) 209
Zsp2I ATGCAT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.