RLG00000027075

regulation of endocannabinoid signaling pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006186
Physical Location & Seq
Forward (+)
8169196 .. 8171048
1853 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027075

Sequence Viewer

Length: 693 bp
ATGGGTTTTATATTGAAGGAGGAGAAGTCGAAGAAGGTCCTGAGGGGCGTGAAGACTCTGTTCTTCTTGATCACCATGTTGATTTCTTTGCTTCTGTTCTCGGCGCCGATTCTTCTCGTCATCGCCGATACGCTTCTCCCCTCGGCGCTTCTCTCGGCTTCTTCCTCGGCCTCTCTCTCTCTCGAAACTCTGTCTTCCCATTTTAGCAACTACGATTTTCGGTACTCGCTCGTTGATATTCCTCTCATCTCCATTATAAGATCCGCCGTTATATTCTGTGTGTATAGCTTCTGCGACGGGCCGAGACTTTCACACGGGCCCTATTTAGGGATCACCACTGTGTGCTCTGTTCTATCCCTCGTGTTTGTTTCATTCAAAGCTTCTTTTGTGTTCGGTGCTCCTGTGGTTTCGGGTTCGGGTGCGGAGGATCAAGGAGGCGAGTATTTGAGAGCCTCCGAGATAGCTCTGTTCATCTGCTCCTTTGCTTTGGCCGTCGGGCATATTGTGGTGGCGTATAGGACGAGTTGCAGAGAAAGAAGGAAGCTTTGGGTTTACAAAATTGACATCGAAGCTATTTCAGCTTGTAAAAATGGGTTTCCGAGCTATTTTGGCTGTGTGATTCCTTCATTATACAGATATTTATTAAGAGGAATCACGATACTGGTGGAGGCAAAATATATAACAGTAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MENTAL

Protein Analysis

231

Amino Acids

25.23

Weight (kDa)

8.95

Isoelectric Point (pI)

29.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
AccB1I GGYRCC 1 cut(s) 103
AciI CCGC 2 cut(s) 264, 422
AclWI GGATC 3 cut(s) 255, 338, 435
AcoI YGGCCR 1 cut(s) 489
AcyI GRCGYC 1 cut(s) 104
AdeI CACNNNGTG 1 cut(s) 342
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 2 cut(s) 326, 327
AgsI TTSAA 2 cut(s) 16, 376
AjuI GAANNNNNNNTTGG 2 cut(s) 529, 561
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 7 cut(s) 288, 380, 464, 544, 572, 581, 603
AluI AGCT 7 cut(s) 288, 380, 464, 544, 572, 581, 603
Alw21I GWGCWC 2 cut(s) 347, 400
Alw26I GTCTC 1 cut(s) 298
AlwI GGATC 3 cut(s) 255, 338, 435
AoxI GGCC 4 cut(s) 168, 299, 317, 489
ApaI GGGCCC 1 cut(s) 321
AspLEI GCGC 2 cut(s) 106, 148
AspS9I GGNCC 4 cut(s) 37, 299, 317, 318
AsuHPI GGTGA 2 cut(s) 64, 325
AvaII GGWCC 1 cut(s) 37
AxyI CCTNAGG 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 321
BanI GGYRCC 1 cut(s) 103
BanII GRGCYC 1 cut(s) 321
BauI CACGAG 1 cut(s) 359
BbsI GAAGAC 2 cut(s) 59, 186
Bbv12I GWGCWC 2 cut(s) 347, 400
BceAI ACGGC 2 cut(s) 251, 476
BclI TGATCA 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 298
BfoI RGCGCY 2 cut(s) 107, 149
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 4 cut(s) 37, 299, 317, 318
BmiI GGNNCC 2 cut(s) 105, 319
BpiI GAAGAC 2 cut(s) 59, 186
BsaHI GRCGYC 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 141, 165
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 327
Bse1I ACTGG 1 cut(s) 666
Bse21I CCTNAGG 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 141, 165
BseLI CCNNNNNNNGG 2 cut(s) 326, 327
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 666
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 321
BshFI GGCC 4 cut(s) 170, 301, 319, 491
BshNI GGYRCC 1 cut(s) 103
BsiHKAI GWGCWC 2 cut(s) 347, 400
BslI CCNNNNNNNGG 2 cut(s) 326, 327
BsmAI GTCTC 1 cut(s) 298
BsnI GGCC 4 cut(s) 170, 301, 319, 491
Bsp120I GGGCCC 1 cut(s) 317
Bsp1286I GDGCHC 3 cut(s) 321, 347, 400
Bsp143I GATC 4 cut(s) 69, 260, 330, 427
BspACI CCGC 2 cut(s) 264, 422
BspANI GGCC 4 cut(s) 170, 301, 319, 491
BspCNI CTCAG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 105, 319
BspPI GGATC 3 cut(s) 255, 338, 435
BspT107I GGYRCC 1 cut(s) 103
BsrI ACTGG 1 cut(s) 666
BssECI CCNNGG 2 cut(s) 141, 165
BssMI GATC 4 cut(s) 69, 260, 330, 427
BssNI GRCGYC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 359
Bst2BI CACGAG 1 cut(s) 359
Bst4CI ACNGT 2 cut(s) 340, 685
BstACI GRCGYC 1 cut(s) 104
BstDEI CTNAG 1 cut(s) 41
BstH2I RGCGCY 2 cut(s) 107, 149
BstHHI GCGC 2 cut(s) 106, 148
BstKTI GATC 4 cut(s) 72, 263, 333, 430
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 4 cut(s) 69, 260, 330, 427
BstMWI GCNNNNNNNGC 2 cut(s) 578, 609
BstSLI GKGCMC 1 cut(s) 321
BstV2I GAAGAC 2 cut(s) 59, 186
BstX2I RGATCY 1 cut(s) 260
BstYI RGATCY 1 cut(s) 260
Bsu36I CCTNAGG 1 cut(s) 41
BsuRI GGCC 4 cut(s) 170, 301, 319, 491
BtgZI GCGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 336
CfoI GCGC 2 cut(s) 106, 148
Cfr13I GGNCC 4 cut(s) 37, 299, 317, 318
Csp6I GTAC 1 cut(s) 223
CviAII CATG 1 cut(s) 76
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 1 cut(s) 41
DinI GGCGCC 1 cut(s) 105
DpnI GATC 4 cut(s) 71, 262, 332, 429
DpnII GATC 4 cut(s) 69, 260, 330, 427
DraIII CACNNNGTG 1 cut(s) 342
EaeI YGGCCR 1 cut(s) 489
EciI GGCGGA 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 37
Eco81I CCTNAGG 1 cut(s) 41
EcoO109I RGGNCCY 2 cut(s) 37, 318
EcoT38I GRGCYC 1 cut(s) 321
EgeI GGCGCC 1 cut(s) 105
EheI GGCGCC 1 cut(s) 105
FaeI CATG 1 cut(s) 79
FatI CATG 1 cut(s) 75
FbaI TGATCA 1 cut(s) 69
FriOI GRGCYC 1 cut(s) 321
GlaI GCGC 2 cut(s) 105, 147
HaeII RGCGCY 2 cut(s) 107, 149
HaeIII GGCC 4 cut(s) 170, 301, 319, 491
HhaI GCGC 2 cut(s) 106, 148
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 1 cut(s) 79
Hin6I GCGC 2 cut(s) 104, 146
HinP1I GCGC 2 cut(s) 104, 146
HindIII AAGCTT 2 cut(s) 378, 542
HinfI GANTC 4 cut(s) 55, 109, 619, 651
HphI GGTGA 2 cut(s) 64, 325
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 2 cut(s) 457, 600
Hpy188III TCNNGA 4 cut(s) 40, 67, 182, 655
Hpy8I GTNNAC 1 cut(s) 553
Hpy99I CGWCG 2 cut(s) 299, 497
HpyAV CCTTC 4 cut(s) 10, 28, 531, 633
HpyCH4III ACNGT 2 cut(s) 340, 685
HpyCH4V TGCA 1 cut(s) 528
HpyF10VI GCNNNNNNNGC 2 cut(s) 578, 609
HpyF3I CTNAG 1 cut(s) 41
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 1 cut(s) 79
HspAI GCGC 2 cut(s) 104, 146
KasI GGCGCC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 4 cut(s) 69, 260, 330, 427
LmnI GCTCC 2 cut(s) 403, 482
LpnPI CCDG 3 cut(s) 53, 414, 647
MalI GATC 4 cut(s) 71, 262, 332, 429
MboI GATC 4 cut(s) 69, 260, 330, 427
MboII GAAGA 6 cut(s) 43, 55, 64, 104, 153, 186
MflI RGATCY 1 cut(s) 260
MhlI GDGCHC 3 cut(s) 321, 347, 400
MluCI AATT 1 cut(s) 558
Mly113I GGCGCC 1 cut(s) 104
MlyI GAGTC 1 cut(s) 49
MseI TTAA 1 cut(s) 644
MslI CAYNNNNRTG 1 cut(s) 338
MwoI GCNNNNNNNGC 2 cut(s) 578, 609
NarI GGCGCC 1 cut(s) 104
NdeII GATC 4 cut(s) 69, 260, 330, 427
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 2 cut(s) 105, 319
NmeAIII GCCGAG 5 cut(s) 80, 122, 134, 146, 327
OliI CACNNNNGTG 1 cut(s) 338
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 3 cut(s) 109, 619, 651
PleI GAGTC 1 cut(s) 49
PluTI GGCGCC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 49
PpuMI RGGWCCY 1 cut(s) 37
PsiI TTATAA 1 cut(s) 257
Psp5II RGGWCCY 1 cut(s) 37
PspN4I GGNNCC 2 cut(s) 105, 319
PspOMI GGGCCC 1 cut(s) 317
PspPI GGNCC 4 cut(s) 37, 299, 317, 318
PspPPI RGGWCCY 1 cut(s) 37
PsuI RGATCY 1 cut(s) 260
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 1 cut(s) 644
Sau3AI GATC 4 cut(s) 69, 260, 330, 427
Sau96I GGNCC 4 cut(s) 37, 299, 317, 318
SchI GAGTC 1 cut(s) 49
SduI GDGCHC 3 cut(s) 321, 347, 400
SetI ASST 8 cut(s) 39, 290, 382, 466, 546, 574, 583, 605
SfoI GGCGCC 1 cut(s) 105
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse9I AATT 1 cut(s) 558
SsiI CCGC 2 cut(s) 264, 422
SspDI GGCGCC 1 cut(s) 103
TaaI ACNGT 2 cut(s) 340, 685
TaqI TCGA 3 cut(s) 29, 183, 567
TasI AATT 1 cut(s) 558
TfiI GAWTC 3 cut(s) 109, 619, 651
Tru1I TTAA 1 cut(s) 644
Tru9I TTAA 1 cut(s) 644
TscAI CASTG 1 cut(s) 343
TspDTI ATGAA 3 cut(s) 360, 460, 615
TspRI CASTG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.