Rh1AG362500

regulation of endocannabinoid signaling pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006186
Physical Location & Seq
Reverse (-)
59725919 .. 59728724
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG362500.1

Sequence Viewer

Length: 615 bp
ATGGGTTTTATATTGAAGGAGGAGAAGTCGAAGAAGGTCCTGAGGGGCGTGAAGACTGTGTTCTTCTTGATCACCATGTTGATTTCTTTGCTTCTGTTCTCGGCGCCGATTCTTCTCGTCATCGCCGATACGCTTCTCCCCTCGGCGCTTCTCTCGGCTTCTTCCTCGGCCTCTCTCTCTCTCGAAACTCTCTCTTCCCATTTTAGCAACTACGATTTTCGGTACTCGCTCGTTGATATTCCTCTCATCTCCATTATAAGATCCGCCGTTATATTCTGTGTTTATAGCTTCTGCGACGGGCCGAGACTTTCCCACGGGCCCTATTTAGGGATCACCACCGTGTGCTCTGTTCTATCCCTCGTGTTTGTTTCGTTCAAAGCTTCTTTTGTGTTCGGTGCTCCGGTGGTTTCGGGTTCGGGTGCGGAGGAGCAAGGAGGAGAGTATTTGAGAGCCTCCGAGATAGCTCTGTTCATCTGCTCTTTTGCTTTGGCCGTCGGGCATATTGTGGTGGCGTATAGGACGAGTTGCAAAGAAAGAAGGAAGCTTTGGGTTTACAAAATTGACATCGAAGCTGTAAGTCCTTTGTTTATCAATTTCTCCATTTCTGGGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MENTAL

Protein Analysis

204

Amino Acids

22.18

Weight (kDa)

8.6

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
AccB1I GGYRCC 1 cut(s) 103
AciI CCGC 2 cut(s) 264, 422
AclWI GGATC 2 cut(s) 255, 338
AcoI YGGCCR 1 cut(s) 489
AcyI GRCGYC 1 cut(s) 104
AdeI CACNNNGTG 1 cut(s) 342
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 3 cut(s) 326, 327, 606
AgsI TTSAA 2 cut(s) 16, 376
AjuI GAANNNNNNNTTGG 2 cut(s) 529, 561
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 5 cut(s) 288, 380, 464, 544, 572
AluI AGCT 5 cut(s) 288, 380, 464, 544, 572
Alw21I GWGCWC 2 cut(s) 347, 400
Alw26I GTCTC 1 cut(s) 298
AlwI GGATC 2 cut(s) 255, 338
AoxI GGCC 4 cut(s) 168, 299, 317, 489
ApaI GGGCCC 1 cut(s) 321
AspLEI GCGC 2 cut(s) 106, 148
AspS9I GGNCC 4 cut(s) 37, 299, 317, 318
AsuHPI GGTGA 2 cut(s) 64, 325
AvaII GGWCC 1 cut(s) 37
AxyI CCTNAGG 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 321
BanI GGYRCC 1 cut(s) 103
BanII GRGCYC 1 cut(s) 321
BauI CACGAG 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 59
Bbv12I GWGCWC 2 cut(s) 347, 400
BceAI ACGGC 2 cut(s) 251, 476
BclI TGATCA 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 298
BfoI RGCGCY 2 cut(s) 107, 149
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 4 cut(s) 37, 299, 317, 318
BmiI GGNNCC 2 cut(s) 105, 319
BpiI GAAGAC 1 cut(s) 59
BsaHI GRCGYC 1 cut(s) 104
BsaJI CCNNGG 3 cut(s) 141, 165, 313
BsaWI WCCGGW 1 cut(s) 400
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 327, 606
Bse21I CCTNAGG 1 cut(s) 41
BseDI CCNNGG 3 cut(s) 141, 165, 313
BseLI CCNNNNNNNGG 3 cut(s) 326, 327, 606
BseMII CTCAG 1 cut(s) 32
BseRI GAGGAG 3 cut(s) 35, 440, 450
BseSI GKGCMC 1 cut(s) 321
BshFI GGCC 4 cut(s) 170, 301, 319, 491
BshNI GGYRCC 1 cut(s) 103
BsiHKAI GWGCWC 2 cut(s) 347, 400
BsiSI CCGG 1 cut(s) 401
BslI CCNNNNNNNGG 3 cut(s) 326, 327, 606
BsmAI GTCTC 1 cut(s) 298
BsnI GGCC 4 cut(s) 170, 301, 319, 491
Bsp120I GGGCCC 1 cut(s) 317
Bsp1286I GDGCHC 3 cut(s) 321, 347, 400
Bsp143I GATC 3 cut(s) 69, 260, 330
BspACI CCGC 2 cut(s) 264, 422
BspANI GGCC 4 cut(s) 170, 301, 319, 491
BspCNI CTCAG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 105, 319
BspPI GGATC 2 cut(s) 255, 338
BspT107I GGYRCC 1 cut(s) 103
BssECI CCNNGG 3 cut(s) 141, 165, 313
BssMI GATC 3 cut(s) 69, 260, 330
BssNI GRCGYC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 359
Bst2BI CACGAG 1 cut(s) 359
Bst4CI ACNGT 2 cut(s) 58, 340
Bst6I CTCTTC 1 cut(s) 199
BstACI GRCGYC 1 cut(s) 104
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 1 cut(s) 313
BstH2I RGCGCY 2 cut(s) 107, 149
BstHHI GCGC 2 cut(s) 106, 148
BstKTI GATC 3 cut(s) 72, 263, 333
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 3 cut(s) 69, 260, 330
BstSLI GKGCMC 1 cut(s) 321
BstV2I GAAGAC 1 cut(s) 59
BstX2I RGATCY 1 cut(s) 260
BstYI RGATCY 1 cut(s) 260
Bsu36I CCTNAGG 1 cut(s) 41
BsuRI GGCC 4 cut(s) 170, 301, 319, 491
BtgI CCRYGG 1 cut(s) 313
BtgZI GCGATG 1 cut(s) 106
CfoI GCGC 2 cut(s) 106, 148
Cfr13I GGNCC 4 cut(s) 37, 299, 317, 318
Csp6I GTAC 1 cut(s) 223
CviAII CATG 1 cut(s) 76
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 1 cut(s) 41
DinI GGCGCC 1 cut(s) 105
DpnI GATC 3 cut(s) 71, 262, 332
DpnII GATC 3 cut(s) 69, 260, 330
DraIII CACNNNGTG 1 cut(s) 342
EaeI YGGCCR 1 cut(s) 489
Eam1104I CTCTTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 199
EciI GGCGGA 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 37
Eco81I CCTNAGG 1 cut(s) 41
EcoO109I RGGNCCY 2 cut(s) 37, 318
EcoT38I GRGCYC 1 cut(s) 321
EgeI GGCGCC 1 cut(s) 105
EheI GGCGCC 1 cut(s) 105
FaeI CATG 1 cut(s) 79
FaiI YATR 8 cut(s) 11, 77, 257, 272, 285, 501, 516, 613
FatI CATG 1 cut(s) 75
FbaI TGATCA 1 cut(s) 69
FriOI GRGCYC 1 cut(s) 321
GlaI GCGC 2 cut(s) 105, 147
HaeII RGCGCY 2 cut(s) 107, 149
HaeIII GGCC 4 cut(s) 170, 301, 319, 491
HapII CCGG 1 cut(s) 401
HhaI GCGC 2 cut(s) 106, 148
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 1 cut(s) 79
Hin6I GCGC 2 cut(s) 104, 146
HinP1I GCGC 2 cut(s) 104, 146
HindIII AAGCTT 2 cut(s) 378, 542
HinfI GANTC 1 cut(s) 109
HpaII CCGG 1 cut(s) 401
HphI GGTGA 2 cut(s) 64, 325
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 1 cut(s) 457
Hpy188III TCNNGA 3 cut(s) 40, 67, 182
Hpy8I GTNNAC 1 cut(s) 553
Hpy99I CGWCG 2 cut(s) 299, 497
HpyAV CCTTC 3 cut(s) 10, 28, 531
HpyCH4III ACNGT 2 cut(s) 58, 340
HpyCH4V TGCA 1 cut(s) 528
HpyF3I CTNAG 1 cut(s) 41
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 1 cut(s) 79
HspAI GCGC 2 cut(s) 104, 146
KasI GGCGCC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 3 cut(s) 69, 260, 330
LmnI GCTCC 2 cut(s) 403, 427
LpnPI CCDG 3 cut(s) 53, 414, 591
MalI GATC 3 cut(s) 71, 262, 332
MboI GATC 3 cut(s) 69, 260, 330
MboII GAAGA 6 cut(s) 43, 55, 64, 104, 153, 186
MflI RGATCY 1 cut(s) 260
MhlI GDGCHC 3 cut(s) 321, 347, 400
MluCI AATT 2 cut(s) 558, 592
Mly113I GGCGCC 1 cut(s) 104
MslI CAYNNNNRTG 1 cut(s) 338
MspI CCGG 1 cut(s) 401
NarI GGCGCC 1 cut(s) 104
NdeII GATC 3 cut(s) 69, 260, 330
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 2 cut(s) 105, 319
NmeAIII GCCGAG 5 cut(s) 80, 122, 134, 146, 327
OliI CACNNNNGTG 1 cut(s) 338
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 1 cut(s) 109
PluTI GGCGCC 1 cut(s) 107
PpuMI RGGWCCY 1 cut(s) 37
PsiI TTATAA 1 cut(s) 257
Psp5II RGGWCCY 1 cut(s) 37
PspN4I GGNNCC 2 cut(s) 105, 319
PspOMI GGGCCC 1 cut(s) 317
PspPI GGNCC 4 cut(s) 37, 299, 317, 318
PspPPI RGGWCCY 1 cut(s) 37
PsuI RGATCY 1 cut(s) 260
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 1 cut(s) 338
Sau3AI GATC 3 cut(s) 69, 260, 330
Sau96I GGNCC 4 cut(s) 37, 299, 317, 318
SduI GDGCHC 3 cut(s) 321, 347, 400
SetI ASST 6 cut(s) 39, 290, 382, 466, 546, 574
SfoI GGCGCC 1 cut(s) 105
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse9I AATT 2 cut(s) 558, 592
SsiI CCGC 2 cut(s) 264, 422
SspDI GGCGCC 1 cut(s) 103
TaaI ACNGT 2 cut(s) 58, 340
TaqI TCGA 3 cut(s) 29, 183, 567
TasI AATT 2 cut(s) 558, 592
TfiI GAWTC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 460
VpaK11BI GGWCC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.