RLG00000027283
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017365
Physical Location & Seq
Reverse (-)
10260036 .. 10290895
30860 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027283

Sequence Viewer

Length: 2454 bp
ATGAGGAGAGCTCTCTGCTCTCTTTCTCGCTCCATTGTCGTTAATTCTTTGTATCCCATATTGATTGAAGAACCGGAGCTTCAGGACTCGGTAGTGATCGATCAGAAGAAGCAGTTGCTTACCATTGTTCCCGAGACTTCCGCTTCATACGGGCTCAGTATCTGTGATGGAAATATTCCTTGTTTCCCAATTTGGAGCTCTGCTGCAAAGAGCATGGCTTCATATGGTGCTAGAACCAAATCTGATAGTCTGCCTCACAATGTTCCTTCATCTCCTCAGACAGAGGTTGAGATCCCTCAGACAGATTTGAGGAGCTTCCACTTTAATGAGCTGCAAACAGCCACCACAAACTTCTGTCGACCTGATAGTATTATGGCTCAAGGTGGTCTTGGTTCTGTCTATAAGGGGTGGATTTATGAGCATTCATTCACAGCTGCCGAGCCTGGTACCAGCATTGCCGTTGTAATAAAGAGGTTTAGCCAACAAAGGACCCTGCAGAGTCAGAAGGACTGGTTGACAGAAGTTAGCTTCCTTGGACACTTGGGTCACCCAAATCTGGTAAAACTGATTGGTTACTGCCTAAAGGATGACATGCGGCTTCAGGTGCATGAATTTATCTCTCAAGGAAGCCTGAACATTCATCTATATGAAAGGGCTTCTCTCTATAAACGACTTTCATGGAACCAGCGTATGAAGATTGCCCTTGGTGCTGCAAAGGGTCTAGCATTTCTTCATAGTAATGAGGCAAAAGTGATTTATGGGGACTTTGAAACTTCCAATATCCTTCTTGATTCAGACTATAATGCTAAACTCTATGGTTATGGTTCGGCCATGGAAAGGCTTCAAGGTTATAGGAGCCATGTCTCAGAAGCGGACATGGCAATGTATGAGGACAAGGTCTTTCCTGTAGCTGCTGCTCACAGTGTGTATGCAGCTCCTGAGACTTCTAATGATCACTTAACCCCGAAAAGTGATGTATACAGTTATGGGGTTGTTCTGCTCGAAATGTTATCCGGCAGACGAGCTTTCGACAGTAGCAGGTCCCTTAGAGAAGAAACTTTACTTCAATTTGCGAAAAAGATTTCTTCGCAAAGTGGTTCTGCCAAGCATGCCAGCAAACGAGACATTGCACACTTTTTTGATGCTCATATTGATGGGCAGTACTCTCAGAACAGGGGTCTCAAAGCACTTAACCTTGCAATTCAGTGCATCTCAAAGGAACCGGAGTCCAGGCCAAACATGAATGAGGTGGTAGAAATATTGGAGCAGATTCAGTATATGCTTTACCTGATTTGTATAAAGCATTGTATTCTTTTTATTTCTGATTTGTTTGGCATGCTTATAAGCTTTGGTGGCAGAGTGATATCTGTTTCATCAAATCAGCTGCCCCTGGCAGCGGATACTATTAGTGTGAATGGACAACCTCTTACTCCTGAGGAGCTTCGGACGTTACAAAGCTGCCCAAACCCTCCAAAGAAGCTAAAACCAGGAAACTATTGGTATGACAAGATTTGTGGACTTTGGGGGAAGGTGAATGTGTTCTGCTTTAGGGTTTTCTTCTTGTTTGCAACCGTTGTTACTTCGATTTATATTACCCTTTTGATAAATCCTGCTGCTACAGAAATTTACTACCCAGGAAGGCTGCGTCATCCAAATCTTGTGAAATTGTTGGGTTATTGCTTTGAAGATGATCATCGGCTTCTGGTGTTTGAATTTATGTCTCCTGGCGGCTTGGATAATCATATATTTGGAAGGGATTCTTGTCTTCAACCACTTTCATGGAACCTGCGTATGAAGATTTCCCTAGAGGCTGCTAATGTTCTAGCATTTCTTCACAATGGTGAGGAAACAGTAATCCATCGGGACATTACAAGTTCTAATATCCTGTTGGATTCAACTTACAATGCCAAACTCGCTGACTTTGGTTTCGCAAAGGATGGACCCGCAGGTGATACAAGACATGTCTCAACAGCAGTGATGGGGACACATGGGTATGCTGCTCCTGAGTATATTGCTACAGGTCATTTAACTGCCAAATGTGATGTATATGGGTTTGGAGTTGTGATGCTAGAAATGCTGTCTAGAAGACGAAGCCTGGACCAAAGCCTGCCGACTGTGCAGCAGAATTTAGTCGGCTGGGCCAAATCTTACCTCGCCAGCAAACGCAGAGTTCTCAAAGTATTTGATCCTCGTTTAGAAGGCCGGTACTCTGTGGCTGGAGCTCTTAAAGCAGCTAATCTTGCAAAACAATGCTTATCAGAAGAACCTGAGTTTAGGCCAAACATGACTGAGGTGGTAAAAGCATTGGAGCAGCTTCAGGAACCTGAAGACATGAAGGGTTTGAAGATTCCTCAAAATGAGCCTTGCCAGAGTCCTCGTGCCAATTCAAGCAATCGCAGGAGAAGCACAAGTTGGATCAGCTTCATGCCATCTGCTTCCCGCAGCTATACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

818

Amino Acids

91.4

Weight (kDa)

8.18

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 125 - 421 5.1e-31 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 145 - 419 1.2e-28 Protein kinase domain
Pkinase PF00069 538 - 767 1.6e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 546 - 769 4.3e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1343
AarI CACCTGC 1 cut(s) 1937
AasI GACNNNNNNGTC 1 cut(s) 543
Acc36I ACCTGC 3 cut(s) 1029, 1794, 1937
Acc65I GGTACC 1 cut(s) 446
AccB1I GGYRCC 1 cut(s) 446
AccI GTMKAC 2 cut(s) 358, 978
AciI CCGC 7 cut(s) 141, 595, 872, 1397, 1728, 1944, 2440
AclWI GGATC 3 cut(s) 286, 2180, 2423
AcoI YGGCCR 1 cut(s) 828
AcsI RAATTY 4 cut(s) 611, 1623, 1712, 2125
AcuI CTGAAG 4 cut(s) 65, 584, 2300, 2346
AdeI CACNNNGTG 1 cut(s) 925
AfaI GTAC 3 cut(s) 448, 1163, 2207
AfiI CCNNNNNNNGG 3 cut(s) 556, 837, 1396
AflIII ACRYGT 1 cut(s) 1960
AjnI CCWGG 7 cut(s) 442, 1229, 1389, 1486, 1633, 1723, 2094
AjuI GAANNNNNNNTTGG 2 cut(s) 2395, 2427
AleI CACNNNNGTG 1 cut(s) 1839
Alw21I GWGCWC 3 cut(s) 13, 200, 2224
Alw26I GTCTC 7 cut(s) 128, 868, 935, 1116, 1184, 1725, 1969
AlwI GGATC 3 cut(s) 286, 2180, 2423
AlwNI CAGNNNCTG 2 cut(s) 162, 938
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 5 cut(s) 828, 1232, 2139, 2200, 2276
ApoI RAATTY 4 cut(s) 611, 1623, 1712, 2125
ArsI GACNNNNNNTTYG 2 cut(s) 1910, 1942
Asp700I GAANNNNTTC 3 cut(s) 840, 1538, 1756
Asp718I GGTACC 1 cut(s) 446
AspS9I GGNCC 5 cut(s) 489, 1041, 1940, 2098, 2139
AsuHPI GGTGA 4 cut(s) 539, 1543, 1853, 1961
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 4 cut(s) 489, 1041, 1940, 2098
AxyI CCTNAGG 1 cut(s) 1434
BanI GGYRCC 1 cut(s) 446
BanII GRGCYC 4 cut(s) 13, 156, 200, 2224
BarI GAAGNNNNNNTAC 2 cut(s) 1044, 1076
BauI CACGAG 1 cut(s) 2376
BbsI GAAGAC 3 cut(s) 1757, 2092, 2334
Bbv12I GWGCWC 3 cut(s) 13, 200, 2224
BccI CCATC 6 cut(s) 161, 1148, 1866, 1931, 1972, 2437
BceAI ACGGC 1 cut(s) 443
BciT130I CCWGG 7 cut(s) 444, 1231, 1391, 1488, 1635, 1725, 2096
BciVI GTATCC 2 cut(s) 63, 1393
BclI TGATCA 2 cut(s) 952, 1690
BcoDI GTCTC 7 cut(s) 128, 868, 935, 1116, 1184, 1725, 1969
BfaI CTAG 6 cut(s) 231, 722, 1805, 1823, 2069, 2082
BfmI CTRYAG 4 cut(s) 494, 906, 1617, 2016
BfuAI ACCTGC 3 cut(s) 1029, 1794, 1937
BfuI GTATCC 2 cut(s) 63, 1393
BmcAI AGTACT 1 cut(s) 1163
Bme1390I CCNGG 7 cut(s) 444, 1231, 1391, 1488, 1635, 1725, 2096
Bme18I GGWCC 4 cut(s) 489, 1041, 1940, 2098
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 5 cut(s) 489, 1041, 1940, 2098, 2139
BmiI GGNNCC 9 cut(s) 448, 491, 683, 857, 1043, 1221, 1784, 1942, 2322
BmrFI CCNGG 7 cut(s) 444, 1231, 1391, 1488, 1635, 1725, 2096
BmsI GCATC 3 cut(s) 1132, 1218, 2055
BpiI GAAGAC 3 cut(s) 1757, 2092, 2334
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 2238
BpuEI CTTGAG 2 cut(s) 363, 606
Bsa29I ATCGAT 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 1692
BsaI GGTCTC 1 cut(s) 1184
BsaJI CCNNGG 5 cut(s) 532, 703, 831, 1389, 1633
BsaWI WCCGGW 2 cut(s) 73, 1222
Bsc4I CCNNNNNNNGG 3 cut(s) 556, 837, 1396
Bse118I RCCGGY 1 cut(s) 2202
Bse1I ACTGG 1 cut(s) 515
Bse21I CCTNAGG 1 cut(s) 1434
Bse3DI GCAATG 3 cut(s) 453, 888, 1125
Bse8I GATNNNNATC 1 cut(s) 1692
BseBI CCWGG 7 cut(s) 444, 1231, 1391, 1488, 1635, 1725, 2096
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 5 cut(s) 532, 703, 831, 1389, 1633
BseGI GGATG 3 cut(s) 592, 1648, 1942
BseJI GATNNNNATC 1 cut(s) 1692
BseLI CCNNNNNNNGG 3 cut(s) 556, 837, 1396
BseMI GCAATG 3 cut(s) 453, 888, 1125
BseNI ACTGG 1 cut(s) 515
BseRI GAGGAG 4 cut(s) 19, 264, 325, 1451
BseYI CCCAGC 1 cut(s) 2136
BsgI GTGCAG 1 cut(s) 2138
BshFI GGCC 5 cut(s) 830, 1234, 2141, 2202, 2278
BshNI GGYRCC 1 cut(s) 446
BshVI ATCGAT 1 cut(s) 99
BsiHKAI GWGCWC 3 cut(s) 13, 200, 2224
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 4 cut(s) 74, 1014, 1223, 2203
BslFI GGGAC 4 cut(s) 776, 1027, 1877, 1996
BslI CCNNNNNNNGG 3 cut(s) 556, 837, 1396
BsmAI GTCTC 7 cut(s) 128, 868, 935, 1116, 1184, 1725, 1969
BsmFI GGGAC 4 cut(s) 776, 1027, 1877, 1996
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 5 cut(s) 830, 1234, 2141, 2202, 2278
Bso31I GGTCTC 1 cut(s) 1184
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 4 cut(s) 13, 156, 200, 2224
Bsp143I GATC 7 cut(s) 96, 100, 291, 952, 1690, 2185, 2415
Bsp19I CCATGG 1 cut(s) 831
BspACI CCGC 7 cut(s) 141, 595, 872, 1397, 1728, 1944, 2440
BspANI GGCC 5 cut(s) 830, 1234, 2141, 2202, 2278
BspDI ATCGAT 1 cut(s) 99
BspLI GGNNCC 9 cut(s) 448, 491, 683, 857, 1043, 1221, 1784, 1942, 2322
BspMAI CTGCAG 1 cut(s) 498
BspMI ACCTGC 3 cut(s) 1029, 1794, 1937
BspPI GGATC 3 cut(s) 286, 2180, 2423
BspT107I GGYRCC 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 1184
BsrDI GCAATG 3 cut(s) 453, 888, 1125
BsrFI RCCGGY 1 cut(s) 2202
BsrI ACTGG 1 cut(s) 515
BssAI RCCGGY 1 cut(s) 2202
BssECI CCNNGG 5 cut(s) 532, 703, 831, 1389, 1633
BssMI GATC 7 cut(s) 96, 100, 291, 952, 1690, 2185, 2415
BssNAI GTATAC 1 cut(s) 979
BssSI CACGAG 1 cut(s) 2376
BssT1I CCWWGG 3 cut(s) 532, 703, 831
Bst1107I GTATAC 1 cut(s) 979
Bst2BI CACGAG 1 cut(s) 2376
Bst2UI CCWGG 7 cut(s) 444, 1231, 1391, 1488, 1635, 1725, 2096
Bst4CI ACNGT 6 cut(s) 923, 983, 1034, 1573, 1852, 2116
BstC8I GCNNGC 5 cut(s) 1110, 1114, 1337, 2108, 2158
BstDSI CCRYGG 1 cut(s) 831
BstEII GGTNACC 1 cut(s) 545
BstF5I GGATG 3 cut(s) 592, 1648, 1942
BstKTI GATC 7 cut(s) 99, 103, 294, 955, 1693, 2188, 2418
BstMAI GTCTC 7 cut(s) 128, 868, 935, 1116, 1184, 1725, 1969
BstMBI GATC 7 cut(s) 96, 100, 291, 952, 1690, 2185, 2415
BstNI CCWGG 7 cut(s) 444, 1231, 1391, 1488, 1635, 1725, 2096
BstNSI RCATGY 4 cut(s) 595, 1112, 1339, 1964
BstPI GGTNACC 1 cut(s) 545
BstSCI CCNGG 7 cut(s) 442, 1229, 1389, 1486, 1633, 1723, 2094
BstSFI CTRYAG 4 cut(s) 494, 906, 1617, 2016
BstV2I GAAGAC 3 cut(s) 1757, 2092, 2334
BstX2I RGATCY 1 cut(s) 291
BstXI CCANNNNNNTGG 1 cut(s) 1779
BstYI RGATCY 1 cut(s) 291
BstZ17I GTATAC 1 cut(s) 979
Bsu15I ATCGAT 1 cut(s) 99
Bsu36I CCTNAGG 1 cut(s) 1434
BsuI GTATCC 2 cut(s) 63, 1393
BsuRI GGCC 5 cut(s) 830, 1234, 2141, 2202, 2278
BsuTUI ATCGAT 1 cut(s) 99
BtgI CCRYGG 1 cut(s) 831
BtsCI GGATG 3 cut(s) 592, 1648, 1942
BtsI GCAGTG 1 cut(s) 1980
BtsIMutI CAGTG 3 cut(s) 928, 1211, 1980
BveI ACCTGC 3 cut(s) 1029, 1794, 1937
Cac8I GCNNGC 5 cut(s) 1110, 1114, 1337, 2108, 2158
CaiI CAGNNNCTG 2 cut(s) 162, 938
Cfr10I RCCGGY 1 cut(s) 2202
Cfr13I GGNCC 5 cut(s) 489, 1041, 1940, 2098, 2139
ClaI ATCGAT 1 cut(s) 99
CseI GACGC 1 cut(s) 1634
Csp6I GTAC 3 cut(s) 447, 1162, 2206
CspCI CAANNNNNGTGG 2 cut(s) 1495, 1530
CviQI GTAC 3 cut(s) 447, 1162, 2206
DpnI GATC 7 cut(s) 98, 102, 293, 954, 1692, 2187, 2417
DpnII GATC 7 cut(s) 96, 100, 291, 952, 1690, 2185, 2415
DraIII CACNNNGTG 1 cut(s) 925
DrdI GACNNNNNNGTC 1 cut(s) 543
DseDI GACNNNNNNGTC 1 cut(s) 543
EaeI YGGCCR 1 cut(s) 828
Ecl136II GAGCTC 3 cut(s) 11, 198, 2222
Eco130I CCWWGG 3 cut(s) 532, 703, 831
Eco24I GRGCYC 4 cut(s) 13, 156, 200, 2224
Eco31I GGTCTC 1 cut(s) 1184
Eco32I GATATC 1 cut(s) 1365
Eco47I GGWCC 4 cut(s) 489, 1041, 1940, 2098
Eco53kI GAGCTC 3 cut(s) 11, 198, 2222
Eco57I CTGAAG 4 cut(s) 65, 584, 2300, 2346
Eco81I CCTNAGG 1 cut(s) 1434
Eco88I CYCGRG 1 cut(s) 131
Eco91I GGTNACC 1 cut(s) 545
EcoICRI GAGCTC 3 cut(s) 11, 198, 2222
EcoO109I RGGNCCY 2 cut(s) 489, 1041
EcoO65I GGTNACC 1 cut(s) 545
EcoRII CCWGG 7 cut(s) 442, 1229, 1389, 1486, 1633, 1723, 2094
EcoRV GATATC 1 cut(s) 1365
EcoT14I CCWWGG 3 cut(s) 532, 703, 831
EcoT38I GRGCYC 4 cut(s) 13, 156, 200, 2224
ErhI CCWWGG 3 cut(s) 532, 703, 831
FalI AAGNNNNNCTT 4 cut(s) 372, 404, 1744, 1776
FaqI GGGAC 4 cut(s) 776, 1027, 1877, 1996
FauI CCCGC 2 cut(s) 1951, 2447
FauNDI CATATG 1 cut(s) 223
FbaI TGATCA 2 cut(s) 952, 1690
FblI GTMKAC 2 cut(s) 358, 978
FokI GGATG 3 cut(s) 599, 1635, 1949
FriOI GRGCYC 4 cut(s) 13, 156, 200, 2224
FspBI CTAG 6 cut(s) 231, 722, 1805, 1823, 2069, 2082
GsaI CCCAGC 1 cut(s) 2140
GsuI CTGGAG 1 cut(s) 2238
HaeIII GGCC 5 cut(s) 830, 1234, 2141, 2202, 2278
HapII CCGG 4 cut(s) 74, 1014, 1223, 2203
HgaI GACGC 1 cut(s) 1634
HincII GTYRAC 2 cut(s) 359, 516
HindII GTYRAC 2 cut(s) 359, 516
HindIII AAGCTT 1 cut(s) 1345
HinfI GANTC 9 cut(s) 86, 499, 791, 1226, 1270, 1757, 1892, 2347, 2371
HpaII CCGG 4 cut(s) 74, 1014, 1223, 2203
HphI GGTGA 4 cut(s) 539, 1543, 1853, 1961
Hpy166II GTNNAC 4 cut(s) 359, 516, 979, 1517
Hpy188III TCNNGA 9 cut(s) 83, 131, 788, 938, 1433, 1862, 2003, 2082, 2318
Hpy8I GTNNAC 4 cut(s) 359, 516, 979, 1517
HpyAV CCTTC 8 cut(s) 276, 499, 794, 1522, 1632, 1746, 2192, 2329
HpyCH4III ACNGT 6 cut(s) 923, 983, 1034, 1573, 1852, 2116
HpyCH4IV ACGT 1 cut(s) 1448
HpySE526I ACGT 1 cut(s) 1448
KpnI GGTACC 1 cut(s) 450
Ksp22I TGATCA 2 cut(s) 952, 1690
Kzo9I GATC 7 cut(s) 96, 100, 291, 952, 1690, 2185, 2415
LweI GCATC 3 cut(s) 1132, 1218, 2055
MaeI CTAG 6 cut(s) 231, 722, 1805, 1823, 2069, 2082
MaeII ACGT 1 cut(s) 1448
MaeIII GTNAC 4 cut(s) 545, 572, 1449, 1576
MalI GATC 7 cut(s) 98, 102, 293, 954, 1692, 2187, 2417
MboI GATC 7 cut(s) 96, 100, 291, 952, 1690, 2185, 2415
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 4 cut(s) 13, 156, 200, 2224
MlyI GAGTC 4 cut(s) 80, 508, 1235, 2380
MmeI TCCRAC 2 cut(s) 1869, 2393
MroXI GAANNNNTTC 3 cut(s) 840, 1538, 1756
MseI TTAA 6 cut(s) 42, 324, 959, 1191, 2027, 2226
MslI CAYNNNNRTG 8 cut(s) 324, 645, 738, 881, 1152, 1777, 1839, 1992
MspA1I CMGCKG 3 cut(s) 434, 1384, 1397
MspI CCGG 4 cut(s) 74, 1014, 1223, 2203
MspR9I CCNGG 7 cut(s) 444, 1231, 1391, 1488, 1635, 1725, 2096
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 7 cut(s) 444, 1231, 1391, 1488, 1635, 1725, 2096
NcoI CCATGG 1 cut(s) 831
NdeI CATATG 1 cut(s) 223
NdeII GATC 7 cut(s) 96, 100, 291, 952, 1690, 2185, 2415
NlaIV GGNNCC 9 cut(s) 448, 491, 683, 857, 1043, 1221, 1784, 1942, 2322
NmeAIII GCCGAG 1 cut(s) 463
NmuCI GTSAC 1 cut(s) 545
NspI RCATGY 4 cut(s) 595, 1112, 1339, 1964
OliI CACNNNNGTG 1 cut(s) 1839
PaeI GCATGC 2 cut(s) 1112, 1339
PaqCI CACCTGC 1 cut(s) 1937
PciI ACATGT 1 cut(s) 1960
PctI GAATGC 1 cut(s) 421
PdmI GAANNNNTTC 3 cut(s) 840, 1538, 1756
PfeI GAWTC 5 cut(s) 791, 1270, 1757, 1892, 2347
PflFI GACNNNGTC 1 cut(s) 896
PleI GAGTC 4 cut(s) 80, 507, 1234, 2379
PpsI GAGTC 4 cut(s) 80, 507, 1234, 2379
PpuMI RGGWCCY 2 cut(s) 489, 1041
PscI ACATGT 1 cut(s) 1960
PsiI TTATAA 1 cut(s) 1343
Psp124BI GAGCTC 3 cut(s) 13, 200, 2224
Psp5II RGGWCCY 2 cut(s) 489, 1041
Psp6I CCWGG 7 cut(s) 442, 1229, 1389, 1486, 1633, 1723, 2094
PspEI GGTNACC 1 cut(s) 545
PspFI CCCAGC 1 cut(s) 2136
PspGI CCWGG 7 cut(s) 442, 1229, 1389, 1486, 1633, 1723, 2094
PspN4I GGNNCC 9 cut(s) 448, 491, 683, 857, 1043, 1221, 1784, 1942, 2322
PspPI GGNCC 5 cut(s) 489, 1041, 1940, 2098, 2139
PspPPI RGGWCCY 2 cut(s) 489, 1041
PstI CTGCAG 1 cut(s) 498
PstNI CAGNNNCTG 2 cut(s) 162, 938
PsuI RGATCY 1 cut(s) 291
PsyI GACNNNGTC 1 cut(s) 896
PvuII CAGCTG 2 cut(s) 434, 1384
RsaI GTAC 3 cut(s) 448, 1163, 2207
RsaNI GTAC 3 cut(s) 447, 1162, 2206
RseI CAYNNNNRTG 8 cut(s) 324, 645, 738, 881, 1152, 1777, 1839, 1992
SacI GAGCTC 3 cut(s) 13, 200, 2224
SalI GTCGAC 1 cut(s) 357
SaqAI TTAA 6 cut(s) 42, 324, 959, 1191, 2027, 2226
Sau3AI GATC 7 cut(s) 96, 100, 291, 952, 1690, 2185, 2415
Sau96I GGNCC 5 cut(s) 489, 1041, 1940, 2098, 2139
ScaI AGTACT 1 cut(s) 1163
SchI GAGTC 4 cut(s) 80, 508, 1235, 2380
ScrFI CCNGG 7 cut(s) 444, 1231, 1391, 1488, 1635, 1725, 2096
SduI GDGCHC 4 cut(s) 13, 156, 200, 2224
SfaNI GCATC 3 cut(s) 1132, 1218, 2055
SfcI CTRYAG 4 cut(s) 494, 906, 1617, 2016
SinI GGWCC 4 cut(s) 489, 1041, 1940, 2098
SmiMI CAYNNNNRTG 8 cut(s) 324, 645, 738, 881, 1152, 1777, 1839, 1992
SmlI CTYRAG 2 cut(s) 378, 621
SmoI CTYRAG 2 cut(s) 378, 621
SphI GCATGC 2 cut(s) 1112, 1339
SsiI CCGC 7 cut(s) 141, 595, 872, 1397, 1728, 1944, 2440
SspI AATATT 2 cut(s) 175, 1260
SspMI CTAG 6 cut(s) 231, 722, 1805, 1823, 2069, 2082
SstI GAGCTC 3 cut(s) 13, 200, 2224
StyD4I CCNGG 7 cut(s) 442, 1229, 1389, 1486, 1633, 1723, 2094
StyI CCWWGG 3 cut(s) 532, 703, 831
TaaI ACNGT 6 cut(s) 923, 983, 1034, 1573, 1852, 2116
TaiI ACGT 1 cut(s) 1451
TaqI TCGA 5 cut(s) 99, 358, 1002, 1029, 1583
TatI WGTACW 1 cut(s) 1161
TauI GCSGC 2 cut(s) 598, 1731
TfiI GAWTC 5 cut(s) 791, 1270, 1757, 1892, 2347
Tru1I TTAA 6 cut(s) 42, 324, 959, 1191, 2027, 2226
Tru9I TTAA 6 cut(s) 42, 324, 959, 1191, 2027, 2226
TscAI CASTG 3 cut(s) 928, 1211, 1980
TseFI GTSAC 1 cut(s) 545
Tsp45I GTSAC 1 cut(s) 545
TspRI CASTG 3 cut(s) 928, 1211, 1980
Tth111I GACNNNGTC 1 cut(s) 896
VpaK11BI GGWCC 4 cut(s) 489, 1041, 1940, 2098
XapI RAATTY 4 cut(s) 611, 1623, 1712, 2125
XbaI TCTAGA 1 cut(s) 2081
XceI RCATGY 4 cut(s) 595, 1112, 1339, 1964
XcmI CCANNNNNNNNNTGG 1 cut(s) 1494
XmiI GTMKAC 2 cut(s) 358, 978
XmnI GAANNNNTTC 3 cut(s) 840, 1538, 1756
XspI CTAG 6 cut(s) 231, 722, 1805, 1823, 2069, 2082
ZrmI AGTACT 1 cut(s) 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.