Rmu_sc0004733.1_g000024

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004733.1
OG0017365
Physical Location & Seq
Reverse (-)
119309 .. 121121
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004733.1_g000024.1.cds

Sequence Viewer

Length: 834 bp
atgcggggcagcagcagtggtgtagggctgcaggtgcacgggtgcataggcggggcagcagcgtgcgcaggtgtgcgcagggaagcagggacgctgcaggggcgggcagcgtggggtggacgcacgggcgcggggctgcaaggcagcgaattgagcgcgggggcgcgctggcaggtatgcaggccgaaagggctgcaggagcagcgggctagcaaggggctaggtgcagggatgattcgatccgagccttgcaacagcaccgatcgcagcccgatcccaacccacctgctgttgcgccgagcaccgacacacaatagcccatccccgcgggccaccagcctcgatctgccctacaccaacgaccagttcttcttccccgtccacgtcaccggccagacaggccgcaagtcacccagacccgtcggaccgtctttctccagaaacagatcagctttgcctcagaccggcctcatccttgcaaccttccacagaacagcacaactgcaagtctcacgggcctccaactccgctcctcgctcagcccggccaccagtcccaccggcgctactccctagccccgcgcagcacaaccccgtgtccttgcagatctcatccgcctgcagatctccgcgcctgcccaacccgaccgtcacatcccagccctggcgctgctgcacaccaggaggccaaatcgacagcaccctctctgcccagaaggagaagagagaaccaggaggaagaaagaagacgcgaaaaggaagaagaaaagaaaaaaaaagaaaagaggaagaaaagaaagaaaaaaacccggcccagaaaaagaaaaataattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

277

Amino Acids

29.76

Weight (kDa)

11.46

Isoelectric Point (pI)

73.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 22, 59, 294
Acc16I TGCGCA 2 cut(s) 67, 77
Acc36I ACCTGC 4 cut(s) 22, 59, 163, 294
AccBSI CCGCTC 1 cut(s) 530
AccII CGCG 7 cut(s) 131, 158, 166, 328, 581, 631, 751
AclWI GGATC 2 cut(s) 234, 268
AcoI YGGCCR 2 cut(s) 391, 545
AfiI CCNNNNNNNGG 2 cut(s) 464, 663
AjiI CACGTC 1 cut(s) 385
AjnI CCWGG 3 cut(s) 662, 679, 730
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 1 cut(s) 452
AluI AGCT 1 cut(s) 452
Alw21I GWGCWC 2 cut(s) 39, 304
Alw26I GTCTC 1 cut(s) 514
Alw44I GTGCAC 1 cut(s) 35
AlwI GGATC 2 cut(s) 234, 268
AoxI GGCC 9 cut(s) 182, 330, 391, 400, 466, 516, 545, 685, 810
ApaLI GTGCAC 1 cut(s) 35
AspS9I GGNCC 4 cut(s) 330, 425, 516, 811
AsuC2I CCSGG 2 cut(s) 544, 809
AsuHPI GGTGA 2 cut(s) 379, 402
AsuNHI GCTAGC 1 cut(s) 209
AvaII GGWCC 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 752
Bbv12I GWGCWC 2 cut(s) 39, 304
BccI CCATC 1 cut(s) 328
BcgI CGANNNNNNTGC 2 cut(s) 175, 209
BciT130I CCWGG 3 cut(s) 664, 681, 732
BcnI CCSGG 2 cut(s) 544, 809
BcoDI GTCTC 1 cut(s) 514
BfaI CTAG 3 cut(s) 210, 221, 573
BfmI CTRYAG 4 cut(s) 29, 95, 194, 619
BfoI RGCGCY 2 cut(s) 566, 670
BfuAI ACCTGC 4 cut(s) 22, 59, 163, 294
BglI GCCNNNNNGGC 2 cut(s) 190, 399
BglII AGATCT 2 cut(s) 606, 623
BlpI GCTNAGC 1 cut(s) 538
Bme1390I CCNGG 5 cut(s) 544, 664, 681, 732, 809
Bme18I GGWCC 1 cut(s) 425
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 4 cut(s) 330, 425, 516, 811
BmrFI CCNGG 5 cut(s) 544, 664, 681, 732, 809
BmtI GCTAGC 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 752
BpmI CTGGAG 1 cut(s) 421
Bpu1102I GCTNAGC 1 cut(s) 538
BpuMI CCSGG 2 cut(s) 544, 809
BsaJI CCNNGG 2 cut(s) 326, 662
Bsc4I CCNNNNNNNGG 2 cut(s) 464, 663
Bse118I RCCGGY 3 cut(s) 389, 464, 559
Bse1I ACTGG 2 cut(s) 364, 551
BseBI CCWGG 3 cut(s) 664, 681, 732
BseDI CCNNGG 2 cut(s) 326, 662
BseGI GGATG 5 cut(s) 237, 320, 471, 611, 653
BseLI CCNNNNNNNGG 2 cut(s) 464, 663
BseMII CTCAG 2 cut(s) 473, 552
BseNI ACTGG 2 cut(s) 364, 551
BsePI GCGCGC 1 cut(s) 164
BseRI GAGGAG 1 cut(s) 522
BseSI GKGCMC 1 cut(s) 39
BseYI CCCAGC 1 cut(s) 657
BsgI GTGCAG 2 cut(s) 246, 658
Bsh1236I CGCG 7 cut(s) 131, 158, 166, 328, 581, 631, 751
Bsh1285I CGRYCG 2 cut(s) 265, 648
BshFI GGCC 9 cut(s) 184, 332, 393, 402, 468, 518, 547, 687, 812
BsiEI CGRYCG 2 cut(s) 265, 648
BsiHKAI GWGCWC 2 cut(s) 39, 304
BsiSI CCGG 5 cut(s) 390, 465, 544, 560, 809
BslFI GGGAC 2 cut(s) 103, 539
BslI CCNNNNNNNGG 2 cut(s) 464, 663
BsmAI GTCTC 1 cut(s) 514
BsmFI GGGAC 2 cut(s) 103, 539
BsnI GGCC 9 cut(s) 184, 332, 393, 402, 468, 518, 547, 687, 812
Bsp1286I GDGCHC 2 cut(s) 39, 304
Bsp143I GATC 7 cut(s) 239, 262, 273, 343, 446, 606, 623
Bsp1720I GCTNAGC 1 cut(s) 538
BspANI GGCC 9 cut(s) 184, 332, 393, 402, 468, 518, 547, 687, 812
BspCNI CTCAG 2 cut(s) 472, 551
BspFNI CGCG 7 cut(s) 131, 158, 166, 328, 581, 631, 751
BspMAI CTGCAG 4 cut(s) 33, 99, 198, 623
BspMI ACCTGC 4 cut(s) 22, 59, 163, 294
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 2 cut(s) 234, 268
BsrBI CCGCTC 1 cut(s) 530
BsrFI RCCGGY 3 cut(s) 389, 464, 559
BsrI ACTGG 2 cut(s) 364, 551
BssAI RCCGGY 3 cut(s) 389, 464, 559
BssECI CCNNGG 2 cut(s) 326, 662
BssHII GCGCGC 1 cut(s) 164
BssMI GATC 7 cut(s) 239, 262, 273, 343, 446, 606, 623
Bst2UI CCWGG 3 cut(s) 664, 681, 732
Bst4CI ACNGT 2 cut(s) 429, 649
Bst6I CTCTTC 1 cut(s) 716
BstDEI CTNAG 2 cut(s) 459, 538
BstDSI CCRYGG 1 cut(s) 326
BstF5I GGATG 5 cut(s) 237, 320, 471, 611, 653
BstFNI CGCG 7 cut(s) 131, 158, 166, 328, 581, 631, 751
BstH2I RGCGCY 2 cut(s) 566, 670
BstKTI GATC 7 cut(s) 242, 265, 276, 346, 449, 609, 626
BstMAI GTCTC 1 cut(s) 514
BstMBI GATC 7 cut(s) 239, 262, 273, 343, 446, 606, 623
BstMCI CGRYCG 2 cut(s) 265, 648
BstMWI GCNNNNNNNGC 9 cut(s) 34, 65, 100, 153, 190, 199, 202, 264, 399
BstNI CCWGG 3 cut(s) 664, 681, 732
BstSCI CCNGG 5 cut(s) 542, 662, 679, 730, 807
BstSFI CTRYAG 4 cut(s) 29, 95, 194, 619
BstSLI GKGCMC 1 cut(s) 39
BstUI CGCG 7 cut(s) 131, 158, 166, 328, 581, 631, 751
BstV2I GAAGAC 1 cut(s) 752
BstX2I RGATCY 2 cut(s) 606, 623
BstYI RGATCY 2 cut(s) 606, 623
BsuRI GGCC 9 cut(s) 184, 332, 393, 402, 468, 518, 547, 687, 812
BtgI CCRYGG 1 cut(s) 326
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 5 cut(s) 237, 320, 471, 611, 653
BtsI GCAGTG 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 22
BveI ACCTGC 4 cut(s) 22, 59, 163, 294
Cfr10I RCCGGY 3 cut(s) 389, 464, 559
Cfr13I GGNCC 4 cut(s) 330, 425, 516, 811
Cfr42I CCGCGG 1 cut(s) 329
CpoI CGGWCCG 1 cut(s) 425
CseI GACGC 3 cut(s) 100, 129, 757
CspI CGGWCCG 1 cut(s) 425
DdeI CTNAG 2 cut(s) 459, 538
DpnI GATC 7 cut(s) 241, 264, 275, 345, 448, 608, 625
DpnII GATC 7 cut(s) 239, 262, 273, 343, 446, 606, 623
EaeI YGGCCR 2 cut(s) 391, 545
Eam1104I CTCTTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 716
EciI GGCGGA 1 cut(s) 604
Eco47I GGWCC 1 cut(s) 425
EcoRII CCWGG 3 cut(s) 662, 679, 730
FaiI YATR 2 cut(s) 47, 178
FaqI GGGAC 2 cut(s) 103, 539
FauI CCCGC 8 cut(s) 44, 96, 124, 151, 198, 321, 333, 586
FokI GGATG 5 cut(s) 244, 307, 458, 598, 640
FspBI CTAG 3 cut(s) 210, 221, 573
FspI TGCGCA 2 cut(s) 67, 77
GsaI CCCAGC 1 cut(s) 661
GsuI CTGGAG 1 cut(s) 421
HaeII RGCGCY 2 cut(s) 566, 670
HaeIII GGCC 9 cut(s) 184, 332, 393, 402, 468, 518, 547, 687, 812
HapII CCGG 5 cut(s) 390, 465, 544, 560, 809
HgaI GACGC 3 cut(s) 100, 129, 757
HinfI GANTC 1 cut(s) 235
HpaII CCGG 5 cut(s) 390, 465, 544, 560, 809
HphI GGTGA 2 cut(s) 379, 402
Hpy166II GTNNAC 3 cut(s) 37, 119, 382
Hpy188I TCNGA 3 cut(s) 244, 425, 462
Hpy188III TCNNGA 1 cut(s) 438
Hpy8I GTNNAC 3 cut(s) 37, 119, 382
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 2 cut(s) 493, 709
HpyCH4III ACNGT 2 cut(s) 429, 649
HpyCH4IV ACGT 1 cut(s) 384
HpyF10VI GCNNNNNNNGC 9 cut(s) 34, 65, 100, 153, 190, 199, 202, 264, 399
HpyF3I CTNAG 2 cut(s) 459, 538
HpySE526I ACGT 1 cut(s) 384
KspI CCGCGG 1 cut(s) 329
Kzo9I GATC 7 cut(s) 239, 262, 273, 343, 446, 606, 623
LmnI GCTCC 2 cut(s) 199, 535
MaeI CTAG 3 cut(s) 210, 221, 573
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 3 cut(s) 385, 408, 649
MalI GATC 7 cut(s) 241, 264, 275, 345, 448, 608, 625
MbiI CCGCTC 1 cut(s) 530
MboI GATC 7 cut(s) 239, 262, 273, 343, 446, 606, 623
MboII GAAGA 8 cut(s) 361, 364, 733, 750, 757, 771, 774, 800
MflI RGATCY 2 cut(s) 606, 623
MhlI GDGCHC 2 cut(s) 39, 304
MluCI AATT 2 cut(s) 149, 829
MmeI TCCRAC 2 cut(s) 403, 546
MnlI CCTC 9 cut(s) 350, 468, 479, 529, 543, 677, 713, 728, 778
MspA1I CMGCKG 2 cut(s) 205, 328
MspI CCGG 5 cut(s) 390, 465, 544, 560, 809
MspR9I CCNGG 5 cut(s) 544, 664, 681, 732, 809
MvaI CCWGG 3 cut(s) 664, 681, 732
MvnI CGCG 7 cut(s) 131, 158, 166, 328, 581, 631, 751
MwoI GCNNNNNNNGC 9 cut(s) 34, 65, 100, 153, 190, 199, 202, 264, 399
NciI CCSGG 2 cut(s) 544, 809
NdeII GATC 7 cut(s) 239, 262, 273, 343, 446, 606, 623
NheI GCTAGC 1 cut(s) 209
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 3 cut(s) 385, 408, 649
NsbI TGCGCA 2 cut(s) 67, 77
PaqCI CACCTGC 3 cut(s) 22, 59, 294
PauI GCGCGC 1 cut(s) 164
PfeI GAWTC 1 cut(s) 235
Ple19I CGATCG 1 cut(s) 265
Psp6I CCWGG 3 cut(s) 662, 679, 730
PspFI CCCAGC 1 cut(s) 657
PspGI CCWGG 3 cut(s) 662, 679, 730
PspPI GGNCC 4 cut(s) 330, 425, 516, 811
PstI CTGCAG 4 cut(s) 33, 99, 198, 623
PsuI RGATCY 2 cut(s) 606, 623
PteI GCGCGC 1 cut(s) 164
PvuI CGATCG 1 cut(s) 265
Rsr2I CGGWCCG 1 cut(s) 425
RsrII CGGWCCG 1 cut(s) 425
SacII CCGCGG 1 cut(s) 329
Sau3AI GATC 7 cut(s) 239, 262, 273, 343, 446, 606, 623
Sau96I GGNCC 4 cut(s) 330, 425, 516, 811
ScrFI CCNGG 5 cut(s) 544, 664, 681, 732, 809
SduI GDGCHC 2 cut(s) 39, 304
SetI ASST 8 cut(s) 36, 73, 177, 226, 288, 387, 454, 485
SfcI CTRYAG 4 cut(s) 29, 95, 194, 619
SfiI GGCCNNNNNGGCC 1 cut(s) 399
Sfr303I CCGCGG 1 cut(s) 329
SgrAI CRCCGGYG 1 cut(s) 559
SgrBI CCGCGG 1 cut(s) 329
SinI GGWCC 1 cut(s) 425
Sse9I AATT 2 cut(s) 149, 829
SspMI CTAG 3 cut(s) 210, 221, 573
StyD4I CCNGG 5 cut(s) 542, 662, 679, 730, 807
TaaI ACNGT 2 cut(s) 429, 649
TaiI ACGT 1 cut(s) 387
TaqI TCGA 3 cut(s) 238, 342, 693
TasI AATT 2 cut(s) 149, 829
TauI GCSGC 1 cut(s) 405
TfiI GAWTC 1 cut(s) 235
TscAI CASTG 1 cut(s) 22
TseFI GTSAC 3 cut(s) 385, 408, 649
Tsp45I GTSAC 3 cut(s) 385, 408, 649
TspRI CASTG 1 cut(s) 22
VneI GTGCAC 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 425
XspI CTAG 3 cut(s) 210, 221, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.