RLG00000027414

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015730
Physical Location & Seq
Forward (+)
11341830 .. 11343039
1210 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027414

Sequence Viewer

Length: 645 bp
ATGTCTTCCTCCTCCAAATCGTCAGAACCCCAACTTGAAAAAACCGATCAAACCAATTTGAAACTCCACTTCCTCTATGACATCAAGAAACGCAAGATTGTTTTTGCGGAGGCAAGCGCAAACTTGGTCGATATCTTATCTGGCCTTTTGGGTTTTCCAATTAGTTCTCTCTCTAAGTTAGAGGTAGGTGCAATGCCTCAAATTTGTAAAAGCTTAGCTAGTCTCGAAGGTGATATAATTCAACCTGGATTCAATAACTGTGAGTTTTTTGAATTGAAACCAACACGTGAGGGATGGCACAACAGAGTCCTCACTTTGTTGAACGGGATTCCTGGAGATGATTTCAAGGACATCCCATTGTTTCTATATCCCTATGATCATGCTAATAGGGATGATGGAGGGATTGTTGCAAGAGGTCGCTTAAGATTCCTCATTTTTGATGACACGAAGATGAAGCCCGCGGGTGATTATCTTGATACCTTCGACTTGGTTAAGGGTATGGGATTTAACTGGGGTGACCTGAAGAAAAAGGAATTGACCCTCACCTCGGAAATGACTCGTTCACTTCTCAAATCTTGTGTGGAGAAAAAGACCGTCCTGACCGAGTTATTTCTGGAAGAGCTGATGAAGGATGAATCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

215

Amino Acids

24.1

Weight (kDa)

5.51

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 461
AciI CCGC 3 cut(s) 107, 459, 461
AcsI RAATTY 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 542
AcvI CACGTG 1 cut(s) 287
AfiI CCNNNNNNNGG 1 cut(s) 547
AflII CTTAAG 1 cut(s) 421
AflIII ACRYGT 1 cut(s) 284
AgsI TTSAA 8 cut(s) 38, 61, 242, 253, 272, 277, 322, 346
AjnI CCWGG 2 cut(s) 244, 331
AluBI AGCT 3 cut(s) 213, 218, 622
AluI AGCT 3 cut(s) 213, 218, 622
Alw26I GTCTC 1 cut(s) 227
AoxI GGCC 1 cut(s) 142
ApoI RAATTY 1 cut(s) 201
AspLEI GCGC 1 cut(s) 119
AsuHPI GGTGA 4 cut(s) 242, 476, 527, 535
BbrPI CACGTG 1 cut(s) 287
BccI CCATC 2 cut(s) 288, 389
BciT130I CCWGG 2 cut(s) 246, 333
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 227
BfaI CTAG 1 cut(s) 219
BfrI CTTAAG 1 cut(s) 421
BlpI GCTNAGC 1 cut(s) 214
Bme1390I CCNGG 2 cut(s) 246, 333
BmrFI CCNGG 2 cut(s) 246, 333
BmrI ACTGGG 1 cut(s) 520
BmuI ACTGGG 1 cut(s) 520
BpmI CTGGAG 1 cut(s) 354
Bpu1102I GCTNAGC 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 459, 546
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 1 cut(s) 515
Bse3DI GCAATG 1 cut(s) 198
BseBI CCWGG 2 cut(s) 246, 333
BseDI CCNNGG 2 cut(s) 459, 546
BseGI GGATG 4 cut(s) 299, 351, 397, 637
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMI GCAATG 1 cut(s) 198
BseNI ACTGG 1 cut(s) 515
Bsh1236I CGCG 1 cut(s) 461
BshFI GGCC 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 1 cut(s) 227
BsnI GGCC 1 cut(s) 144
Bsp143I GATC 2 cut(s) 46, 376
Bsp1720I GCTNAGC 1 cut(s) 214
BspACI CCGC 3 cut(s) 107, 459, 461
BspANI GGCC 1 cut(s) 144
BspFNI CGCG 1 cut(s) 461
BspQI GCTCTTC 1 cut(s) 612
BspTI CTTAAG 1 cut(s) 421
BsrDI GCAATG 1 cut(s) 198
BsrI ACTGG 1 cut(s) 515
BssECI CCNNGG 2 cut(s) 459, 546
BssMI GATC 2 cut(s) 46, 376
Bst2UI CCWGG 2 cut(s) 246, 333
Bst4CI ACNGT 2 cut(s) 260, 595
Bst6I CTCTTC 1 cut(s) 612
BstAFI CTTAAG 1 cut(s) 421
BstBAI YACGTR 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 115, 459
BstDEI CTNAG 2 cut(s) 174, 214
BstDSI CCRYGG 1 cut(s) 459
BstEII GGTNACC 1 cut(s) 515
BstF5I GGATG 4 cut(s) 299, 351, 397, 637
BstFNI CGCG 1 cut(s) 461
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 2 cut(s) 49, 379
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 2 cut(s) 46, 376
BstNI CCWGG 2 cut(s) 246, 333
BstPI GGTNACC 1 cut(s) 515
BstSCI CCNGG 2 cut(s) 244, 331
BstUI CGCG 1 cut(s) 461
BsuRI GGCC 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 4 cut(s) 299, 351, 397, 637
Cac8I GCNNGC 2 cut(s) 115, 459
CfoI GCGC 1 cut(s) 119
Cfr42I CCGCGG 1 cut(s) 462
CviAII CATG 2 cut(s) 380, 642
CviJI RGCY 5 cut(s) 144, 213, 218, 457, 622
CviKI_1 RGCY 5 cut(s) 144, 213, 218, 457, 622
DdeI CTNAG 2 cut(s) 174, 214
DpnI GATC 2 cut(s) 48, 378
DpnII GATC 2 cut(s) 46, 376
Eam1104I CTCTTC 1 cut(s) 612
EarI CTCTTC 1 cut(s) 612
Eco32I GATATC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 542
Eco72I CACGTG 1 cut(s) 287
Eco91I GGTNACC 1 cut(s) 515
EcoO65I GGTNACC 1 cut(s) 515
EcoRII CCWGG 2 cut(s) 244, 331
EcoRV GATATC 1 cut(s) 133
FaeI CATG 2 cut(s) 383, 645
FaiI YATR 7 cut(s) 78, 236, 367, 375, 381, 500, 643
FatI CATG 2 cut(s) 379, 641
FauI CCCGC 2 cut(s) 454, 466
FbaI TGATCA 1 cut(s) 376
FokI GGATG 3 cut(s) 306, 338, 404
FspBI CTAG 1 cut(s) 219
GlaI GCGC 1 cut(s) 118
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 1 cut(s) 144
HhaI GCGC 1 cut(s) 119
Hin1II CATG 2 cut(s) 383, 645
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HindIII AAGCTT 1 cut(s) 211
HinfI GANTC 6 cut(s) 249, 306, 328, 426, 556, 635
HphI GGTGA 4 cut(s) 242, 476, 527, 535
Hpy166II GTNNAC 1 cut(s) 563
Hpy188I TCNGA 2 cut(s) 25, 550
Hpy188III TCNNGA 5 cut(s) 85, 224, 473, 598, 614
Hpy8I GTNNAC 1 cut(s) 563
HpyAV CCTTC 3 cut(s) 221, 490, 622
HpyCH4III ACNGT 2 cut(s) 260, 595
HpyCH4IV ACGT 1 cut(s) 286
HpyCH4V TGCA 3 cut(s) 191, 410, 641
HpyF3I CTNAG 2 cut(s) 174, 214
HpySE526I ACGT 1 cut(s) 286
Hsp92II CATG 2 cut(s) 383, 645
HspAI GCGC 1 cut(s) 117
Ksp22I TGATCA 1 cut(s) 376
KspI CCGCGG 1 cut(s) 462
Kzo9I GATC 2 cut(s) 46, 376
LguI GCTCTTC 1 cut(s) 612
LpnPI CCDG 9 cut(s) 126, 231, 258, 318, 345, 496, 533, 599, 611
MaeI CTAG 1 cut(s) 219
MaeII ACGT 1 cut(s) 286
MaeIII GTNAC 1 cut(s) 515
MalI GATC 2 cut(s) 48, 378
MboI GATC 2 cut(s) 46, 376
MboII GAAGA 3 cut(s) 460, 535, 629
MluCI AATT 6 cut(s) 55, 159, 201, 237, 272, 533
MlyI GAGTC 2 cut(s) 315, 550
MseI TTAA 3 cut(s) 422, 492, 507
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 461
MspCI CTTAAG 1 cut(s) 421
MspR9I CCNGG 2 cut(s) 246, 333
MvaI CCWGG 2 cut(s) 246, 333
MvnI CGCG 1 cut(s) 461
NdeII GATC 2 cut(s) 46, 376
NlaIII CATG 2 cut(s) 383, 645
NmuCI GTSAC 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 612
PfeI GAWTC 4 cut(s) 249, 328, 426, 635
PfoI TCCNGGA 1 cut(s) 331
PleI GAGTC 2 cut(s) 314, 550
PmaCI CACGTG 1 cut(s) 287
PmlI CACGTG 1 cut(s) 287
PpsI GAGTC 2 cut(s) 314, 550
Ppu21I YACGTR 1 cut(s) 287
Psp6I CCWGG 2 cut(s) 244, 331
PspCI CACGTG 1 cut(s) 287
PspEI GGTNACC 1 cut(s) 515
PspGI CCWGG 2 cut(s) 244, 331
RseI CAYNNNNRTG 1 cut(s) 449
SacII CCGCGG 1 cut(s) 462
SapI GCTCTTC 1 cut(s) 612
SaqAI TTAA 3 cut(s) 422, 492, 507
Sau3AI GATC 2 cut(s) 46, 376
SchI GAGTC 2 cut(s) 315, 550
ScrFI CCNGG 2 cut(s) 246, 333
Sfr303I CCGCGG 1 cut(s) 462
SgrBI CCGCGG 1 cut(s) 462
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
Sse9I AATT 6 cut(s) 55, 159, 201, 237, 272, 533
SsiI CCGC 3 cut(s) 107, 459, 461
SspMI CTAG 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 244, 331
TaaI ACNGT 2 cut(s) 260, 595
TaiI ACGT 1 cut(s) 289
TaqI TCGA 3 cut(s) 129, 225, 483
TaqII GACCGA 1 cut(s) 617
TasI AATT 6 cut(s) 55, 159, 201, 237, 272, 533
TfiI GAWTC 4 cut(s) 249, 328, 426, 635
Tru1I TTAA 3 cut(s) 422, 492, 507
Tru9I TTAA 3 cut(s) 422, 492, 507
TseFI GTSAC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 2 cut(s) 467, 641
Vha464I CTTAAG 1 cut(s) 421
XapI RAATTY 1 cut(s) 201
XspI CTAG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.