RLG00000027475

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015730
Physical Location & Seq
Forward (+)
12030592 .. 12031577
986 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027475

Sequence Viewer

Length: 744 bp
ATGTCTTCCTCCTCCAAATCGTCAGAAACCCAACTTGAAAAAACCCATCAAACCAATTTGAAACTCCACTTCCTCTATGACATCAAGAAACGCAAGATTGTTTTGGCGGAGGCAAGGGCCAACTTGGTCAATATCTTATTTGGCTTTTTGGGTTTTCCAATTAGTTCTCTCTCTAAGTTAGAGGTAGGTGCAACGCCTCAAATTTGTAAAAGCTTAGCTAGTCTCGAAGGTGATATAATTCAACCTGGATTCAATAACTGTGAGTTTTTTGAATTGAAACCAACACGTGAGGGATGGCACAACAGAGTCCTCACTTTGTTGAACGGGATTCCTGGAGATGATTTCAAGGACATCCCATTGTTTCTATGTCGGAACTGTACTTTTGAGATTCGGCTTATTCCTTACTACGTCACAACGGATCCGGCAACGCTTTGCGGGACTTGCAGAAACCCTATGGACAAGGAGGTTCCCTATGATCATGCTAATAGGGATGATAGAGGGATTGTTGCAAGAGGTCGCTTGAGATTCCTCATTTTTGATGACATGAAGATGAAGCCCGCGAGTGATTATCTTGATACCTTCGACTTGGTTAAGGGTATGGGATTTAACTGGGGTGAGCTGAAGAAAAAGGAATTGATCCTCACCTCGGAAATGACTCATTCACTGCTCAAATCTTGTGTGGAGAAAAAGACCGTCCTGACAGAGTTATTTCTGGAAGAGCTGATGAAGGATGAGTCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

248

Amino Acids

28.14

Weight (kDa)

6.54

Isoelectric Point (pI)

28.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 18 - 241 3.5e-17 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 560
AciI CCGC 3 cut(s) 107, 435, 558
AclWI GGATC 3 cut(s) 413, 426, 631
AcsI RAATTY 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 641
AcvI CACGTG 1 cut(s) 287
AfaI GTAC 1 cut(s) 379
AfiI CCNNNNNNNGG 1 cut(s) 646
AflIII ACRYGT 1 cut(s) 284
AgsI TTSAA 8 cut(s) 38, 61, 242, 253, 272, 277, 322, 346
AjnI CCWGG 2 cut(s) 244, 331
AluBI AGCT 4 cut(s) 213, 218, 619, 721
AluI AGCT 4 cut(s) 213, 218, 619, 721
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 3 cut(s) 413, 426, 631
AoxI GGCC 1 cut(s) 117
ApoI RAATTY 1 cut(s) 201
AspS9I GGNCC 1 cut(s) 117
AsuHPI GGTGA 3 cut(s) 242, 626, 634
BamHI GGATCC 1 cut(s) 418
BbrPI CACGTG 1 cut(s) 287
BccI CCATC 2 cut(s) 54, 288
BciT130I CCWGG 2 cut(s) 246, 333
BclI TGATCA 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 227
BfaI CTAG 1 cut(s) 219
BlpI GCTNAGC 1 cut(s) 214
Bme1390I CCNGG 2 cut(s) 246, 333
BmgT120I GGNCC 1 cut(s) 117
BmiI GGNNCC 2 cut(s) 420, 468
BmrFI CCNGG 2 cut(s) 246, 333
BmrI ACTGGG 1 cut(s) 619
BmuI ACTGGG 1 cut(s) 619
BpmI CTGGAG 1 cut(s) 354
Bpu1102I GCTNAGC 1 cut(s) 214
BpuEI CTTGAG 1 cut(s) 541
BsaAI YACGTR 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 645
Bsc4I CCNNNNNNNGG 1 cut(s) 646
Bse1I ACTGG 1 cut(s) 614
BseBI CCWGG 2 cut(s) 246, 333
BseDI CCNNGG 1 cut(s) 645
BseGI GGATG 4 cut(s) 299, 351, 496, 736
BseLI CCNNNNNNNGG 1 cut(s) 646
BseNI ACTGG 1 cut(s) 614
Bsh1236I CGCG 1 cut(s) 560
BshFI GGCC 1 cut(s) 119
BsiSI CCGG 1 cut(s) 422
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 1 cut(s) 646
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 451
BsnI GGCC 1 cut(s) 119
Bsp143I GATC 3 cut(s) 418, 475, 636
Bsp1720I GCTNAGC 1 cut(s) 214
BspACI CCGC 3 cut(s) 107, 435, 558
BspANI GGCC 1 cut(s) 119
BspFNI CGCG 1 cut(s) 560
BspLI GGNNCC 2 cut(s) 420, 468
BspPI GGATC 3 cut(s) 413, 426, 631
BspQI GCTCTTC 1 cut(s) 711
BsrI ACTGG 1 cut(s) 614
BssECI CCNNGG 1 cut(s) 645
BssMI GATC 3 cut(s) 418, 475, 636
Bst2UI CCWGG 2 cut(s) 246, 333
Bst4CI ACNGT 3 cut(s) 260, 377, 694
Bst6I CTCTTC 1 cut(s) 711
BstBAI YACGTR 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 2 cut(s) 174, 214
BstF5I GGATG 4 cut(s) 299, 351, 496, 736
BstFNI CGCG 1 cut(s) 560
BstKTI GATC 3 cut(s) 421, 478, 639
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 3 cut(s) 418, 475, 636
BstMWI GCNNNNNNNGC 1 cut(s) 441
BstNI CCWGG 2 cut(s) 246, 333
BstSCI CCNGG 2 cut(s) 244, 331
BstUI CGCG 1 cut(s) 560
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BsuRI GGCC 1 cut(s) 119
BtsCI GGATG 4 cut(s) 299, 351, 496, 736
BtsI GCAGTG 1 cut(s) 662
BtsIMutI CAGTG 1 cut(s) 662
Cac8I GCNNGC 1 cut(s) 558
Cfr13I GGNCC 1 cut(s) 117
Csp6I GTAC 1 cut(s) 378
CviAII CATG 3 cut(s) 479, 544, 741
CviJI RGCY 8 cut(s) 119, 144, 213, 218, 394, 556, 619, 721
CviKI_1 RGCY 8 cut(s) 119, 144, 213, 218, 394, 556, 619, 721
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 2 cut(s) 174, 214
DpnI GATC 3 cut(s) 420, 477, 638
DpnII GATC 3 cut(s) 418, 475, 636
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
EciI GGCGGA 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 641
Eco72I CACGTG 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 244, 331
FaeI CATG 3 cut(s) 482, 547, 744
FaiI YATR 9 cut(s) 78, 236, 367, 455, 474, 480, 545, 599, 742
FaqI GGGAC 1 cut(s) 451
FatI CATG 3 cut(s) 478, 543, 740
FauI CCCGC 2 cut(s) 428, 565
FbaI TGATCA 1 cut(s) 475
FokI GGATG 3 cut(s) 306, 338, 503
FspBI CTAG 1 cut(s) 219
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 1 cut(s) 119
HapII CCGG 1 cut(s) 422
Hin1II CATG 3 cut(s) 482, 547, 744
HindIII AAGCTT 1 cut(s) 211
HinfI GANTC 7 cut(s) 249, 306, 328, 388, 525, 655, 734
HpaII CCGG 1 cut(s) 422
HphI GGTGA 3 cut(s) 242, 626, 634
Hpy188I TCNGA 3 cut(s) 25, 372, 649
Hpy188III TCNNGA 5 cut(s) 85, 224, 572, 697, 713
HpyAV CCTTC 3 cut(s) 221, 589, 721
HpyCH4III ACNGT 3 cut(s) 260, 377, 694
HpyCH4IV ACGT 2 cut(s) 286, 408
HpyCH4V TGCA 4 cut(s) 191, 444, 509, 740
HpyF10VI GCNNNNNNNGC 1 cut(s) 441
HpyF3I CTNAG 2 cut(s) 174, 214
HpySE526I ACGT 2 cut(s) 286, 408
Hsp92II CATG 3 cut(s) 482, 547, 744
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 3 cut(s) 418, 475, 636
LguI GCTCTTC 1 cut(s) 711
LpnPI CCDG 8 cut(s) 231, 258, 318, 345, 435, 595, 698, 710
MaeI CTAG 1 cut(s) 219
MaeII ACGT 2 cut(s) 286, 408
MaeIII GTNAC 1 cut(s) 409
MalI GATC 3 cut(s) 420, 477, 638
MboI GATC 3 cut(s) 418, 475, 636
MboII GAAGA 3 cut(s) 559, 634, 728
MflI RGATCY 1 cut(s) 418
MluCI AATT 6 cut(s) 55, 159, 201, 237, 272, 632
MlyI GAGTC 3 cut(s) 315, 649, 743
MmeI TCCRAC 1 cut(s) 350
MseI TTAA 2 cut(s) 591, 606
MslI CAYNNNNRTG 1 cut(s) 548
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 2 cut(s) 246, 333
MvaI CCWGG 2 cut(s) 246, 333
MvnI CGCG 1 cut(s) 560
MwoI GCNNNNNNNGC 1 cut(s) 441
NdeII GATC 3 cut(s) 418, 475, 636
NlaIII CATG 3 cut(s) 482, 547, 744
NlaIV GGNNCC 2 cut(s) 420, 468
NmuCI GTSAC 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 711
PfeI GAWTC 4 cut(s) 249, 328, 388, 525
PfoI TCCNGGA 1 cut(s) 331
PleI GAGTC 3 cut(s) 314, 649, 742
PmaCI CACGTG 1 cut(s) 287
PmlI CACGTG 1 cut(s) 287
PpsI GAGTC 3 cut(s) 314, 649, 742
Ppu21I YACGTR 1 cut(s) 287
Psp6I CCWGG 2 cut(s) 244, 331
PspCI CACGTG 1 cut(s) 287
PspGI CCWGG 2 cut(s) 244, 331
PspN4I GGNNCC 2 cut(s) 420, 468
PspPI GGNCC 1 cut(s) 117
PsuI RGATCY 1 cut(s) 418
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
RseI CAYNNNNRTG 1 cut(s) 548
SapI GCTCTTC 1 cut(s) 711
SaqAI TTAA 2 cut(s) 591, 606
Sau3AI GATC 3 cut(s) 418, 475, 636
Sau96I GGNCC 1 cut(s) 117
SchI GAGTC 3 cut(s) 315, 649, 743
ScrFI CCNGG 2 cut(s) 246, 333
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 6 cut(s) 55, 159, 201, 237, 272, 632
SsiI CCGC 3 cut(s) 107, 435, 558
SspMI CTAG 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 244, 331
TaaI ACNGT 3 cut(s) 260, 377, 694
TaiI ACGT 2 cut(s) 289, 411
TaqI TCGA 2 cut(s) 225, 582
TasI AATT 6 cut(s) 55, 159, 201, 237, 272, 632
TatI WGTACW 1 cut(s) 377
TfiI GAWTC 4 cut(s) 249, 328, 388, 525
Tru1I TTAA 2 cut(s) 591, 606
Tru9I TTAA 2 cut(s) 591, 606
TscAI CASTG 1 cut(s) 669
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 3 cut(s) 560, 566, 740
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 1 cut(s) 669
XapI RAATTY 1 cut(s) 201
XspI CTAG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.