Rw0G001540

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00044
OG0015730
Physical Location & Seq
Reverse (-)
58866 .. 59719
854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G001540.1

Sequence Viewer

Length: 645 bp
ATGGCGGAGGCAGGAGCCAACTTGGTCGATATCTTATTTGGCTTTTTGGGTTTTCCAATTAGTTCTCTCTCTAAGTTAGAGGTAGGTGCAATGCCTCAAATTTGTAAAAGCTTAGCTAGTCTGGACGGTGATATAATTCAACCTGGATTCAATAACAGTGAGTTTTTTGAATTGAAACCAACACATGAGGGATGGCACAACAGAGTCCTCACTTTGTTGAACGGGATTCCTGCTGGAGATGATTTCAAGGACATCCCATTGTTTCTATGTCGGAACTGTACTTTTGAGATTCGGCTTATTCCTTACTACGTCACAACGGATCCGGCAACGCTTTGCGGGAATTGCAGAAACCCTATGGACAAGGAGGTTCCCTATGATCATGCTAATAGGGATGATAGAGGGATTGTTGCAAGAAGTAGCTTAAGATTCCTCATTTTTGATGACATGAAGATGAAGCCCGCGGGTGATTATCTTGATACCTTCGACTTGCTTAAGGGTATGGGATATAACGGGGGTGAGCTGAAGAAAAAGGAATTGACCCTCACCTCGGAAATGACTCGTTCACTGCTCAAATCTTGTGTGGAGAAAAAGACCGTCCTGAACGAGTTATTTCTGGAAGAGCTGATGAAGGATGAATCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

214

Amino Acids

23.99

Weight (kDa)

4.98

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 80 - 208 1.8e-08 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 461
AciI CCGC 4 cut(s) 5, 336, 459, 461
AclWI GGATC 2 cut(s) 314, 327
AcsI RAATTY 1 cut(s) 99
AcuI CTGAAG 1 cut(s) 542
AfaI GTAC 1 cut(s) 280
AfiI CCNNNNNNNGG 1 cut(s) 547
AflII CTTAAG 2 cut(s) 421, 491
AgsI TTSAA 6 cut(s) 140, 151, 170, 175, 220, 247
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 5 cut(s) 111, 116, 420, 520, 622
AluI AGCT 5 cut(s) 111, 116, 420, 520, 622
AlwI GGATC 2 cut(s) 314, 327
ApoI RAATTY 1 cut(s) 99
AsuHPI GGTGA 4 cut(s) 140, 476, 527, 535
BamHI GGATCC 1 cut(s) 319
BccI CCATC 1 cut(s) 186
BciT130I CCWGG 1 cut(s) 144
BclI TGATCA 1 cut(s) 376
BfaI CTAG 1 cut(s) 117
BfrI CTTAAG 2 cut(s) 421, 491
BlpI GCTNAGC 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 144
BmiI GGNNCC 3 cut(s) 16, 321, 369
BmrFI CCNGG 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 255
Bpu1102I GCTNAGC 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 459, 546
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 459, 546
BseGI GGATG 4 cut(s) 197, 252, 397, 637
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMI GCAATG 1 cut(s) 96
Bsh1236I CGCG 1 cut(s) 461
BsiSI CCGG 1 cut(s) 323
BslI CCNNNNNNNGG 1 cut(s) 547
Bsp143I GATC 2 cut(s) 319, 376
Bsp1720I GCTNAGC 1 cut(s) 112
BspACI CCGC 4 cut(s) 5, 336, 459, 461
BspFNI CGCG 1 cut(s) 461
BspLI GGNNCC 3 cut(s) 16, 321, 369
BspPI GGATC 2 cut(s) 314, 327
BspQI GCTCTTC 1 cut(s) 612
BspTI CTTAAG 2 cut(s) 421, 491
BsrDI GCAATG 1 cut(s) 96
BssECI CCNNGG 2 cut(s) 459, 546
BssMI GATC 2 cut(s) 319, 376
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 4 cut(s) 128, 158, 278, 595
Bst6I CTCTTC 1 cut(s) 612
BstAFI CTTAAG 2 cut(s) 421, 491
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 2 cut(s) 72, 112
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 4 cut(s) 197, 252, 397, 637
BstFNI CGCG 1 cut(s) 461
BstKTI GATC 2 cut(s) 322, 379
BstMBI GATC 2 cut(s) 319, 376
BstMWI GCNNNNNNNGC 1 cut(s) 342
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstUI CGCG 1 cut(s) 461
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 4 cut(s) 197, 252, 397, 637
BtsI GCAGTG 1 cut(s) 563
BtsIMutI CAGTG 2 cut(s) 163, 563
Cac8I GCNNGC 1 cut(s) 459
Cfr42I CCGCGG 1 cut(s) 462
Csp6I GTAC 1 cut(s) 279
CviAII CATG 4 cut(s) 185, 380, 445, 642
CviJI RGCY 9 cut(s) 17, 42, 111, 116, 295, 420, 457, 520, 622
CviKI_1 RGCY 9 cut(s) 17, 42, 111, 116, 295, 420, 457, 520, 622
CviQI GTAC 1 cut(s) 279
DdeI CTNAG 2 cut(s) 72, 112
DpnI GATC 2 cut(s) 321, 378
DpnII GATC 2 cut(s) 319, 376
Eam1104I CTCTTC 1 cut(s) 612
EarI CTCTTC 1 cut(s) 612
EciI GGCGGA 1 cut(s) 20
Eco32I GATATC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 142
EcoRV GATATC 1 cut(s) 31
FaeI CATG 4 cut(s) 188, 383, 448, 645
FatI CATG 4 cut(s) 184, 379, 444, 641
FauI CCCGC 3 cut(s) 329, 454, 466
FbaI TGATCA 1 cut(s) 376
FokI GGATG 3 cut(s) 204, 239, 404
FspBI CTAG 1 cut(s) 117
GsuI CTGGAG 1 cut(s) 255
HapII CCGG 1 cut(s) 323
Hin1II CATG 4 cut(s) 188, 383, 448, 645
HindIII AAGCTT 1 cut(s) 109
HinfI GANTC 7 cut(s) 147, 204, 226, 289, 426, 556, 635
HpaII CCGG 1 cut(s) 323
HphI GGTGA 4 cut(s) 140, 476, 527, 535
Hpy166II GTNNAC 1 cut(s) 563
Hpy188I TCNGA 2 cut(s) 273, 550
Hpy188III TCNNGA 4 cut(s) 122, 473, 598, 614
Hpy8I GTNNAC 1 cut(s) 563
HpyAV CCTTC 2 cut(s) 490, 622
HpyCH4III ACNGT 4 cut(s) 128, 158, 278, 595
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 4 cut(s) 89, 345, 410, 641
HpyF10VI GCNNNNNNNGC 1 cut(s) 342
HpyF3I CTNAG 2 cut(s) 72, 112
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 4 cut(s) 188, 383, 448, 645
Ksp22I TGATCA 1 cut(s) 376
KspI CCGCGG 1 cut(s) 462
Kzo9I GATC 2 cut(s) 319, 376
LguI GCTCTTC 1 cut(s) 612
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 8 cut(s) 107, 129, 156, 219, 243, 336, 599, 611
MaeI CTAG 1 cut(s) 117
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 1 cut(s) 310
MalI GATC 2 cut(s) 321, 378
MboI GATC 2 cut(s) 319, 376
MboII GAAGA 3 cut(s) 460, 535, 629
MflI RGATCY 1 cut(s) 319
MluCI AATT 6 cut(s) 57, 99, 135, 170, 340, 533
MlyI GAGTC 2 cut(s) 213, 550
MmeI TCCRAC 1 cut(s) 251
MnlI CCTC 9 cut(s) 73, 105, 181, 218, 358, 392, 440, 551, 556
MseI TTAA 2 cut(s) 422, 492
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 461
MspCI CTTAAG 2 cut(s) 421, 491
MspI CCGG 1 cut(s) 323
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 1 cut(s) 342
NdeII GATC 2 cut(s) 319, 376
NlaIII CATG 4 cut(s) 188, 383, 448, 645
NlaIV GGNNCC 3 cut(s) 16, 321, 369
NmuCI GTSAC 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 612
PfeI GAWTC 5 cut(s) 147, 226, 289, 426, 635
PleI GAGTC 2 cut(s) 212, 550
PpsI GAGTC 2 cut(s) 212, 550
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 3 cut(s) 16, 321, 369
PsuI RGATCY 1 cut(s) 319
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
RseI CAYNNNNRTG 1 cut(s) 449
SacII CCGCGG 1 cut(s) 462
SapI GCTCTTC 1 cut(s) 612
SaqAI TTAA 2 cut(s) 422, 492
Sau3AI GATC 2 cut(s) 319, 376
SchI GAGTC 2 cut(s) 213, 550
ScrFI CCNGG 1 cut(s) 144
Sfr303I CCGCGG 1 cut(s) 462
SgrBI CCGCGG 1 cut(s) 462
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 2 cut(s) 421, 491
SmoI CTYRAG 2 cut(s) 421, 491
Sse9I AATT 6 cut(s) 57, 99, 135, 170, 340, 533
SsiI CCGC 4 cut(s) 5, 336, 459, 461
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 142
TaaI ACNGT 4 cut(s) 128, 158, 278, 595
TaiI ACGT 1 cut(s) 312
TaqI TCGA 2 cut(s) 27, 483
TasI AATT 6 cut(s) 57, 99, 135, 170, 340, 533
TatI WGTACW 1 cut(s) 278
TfiI GAWTC 5 cut(s) 147, 226, 289, 426, 635
Tru1I TTAA 2 cut(s) 422, 492
Tru9I TTAA 2 cut(s) 422, 492
TscAI CASTG 2 cut(s) 163, 570
TseFI GTSAC 1 cut(s) 310
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 3 cut(s) 461, 467, 641
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 2 cut(s) 163, 570
Vha464I CTTAAG 2 cut(s) 421, 491
XapI RAATTY 1 cut(s) 99
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.