RLG00000027415

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015730
Physical Location & Seq
Reverse (-)
11343334 .. 11343954
621 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027415

Sequence Viewer

Length: 621 bp
ATGTCTTCCTCCTCCAAATCGTCAGAACCCCAACTTGAAAAAACCGATCAAACCAATTTGAAACTCCACTTCCTCTATGACATCAAGAAACGCAAGATTGTTTTTGCGGAGGCAAGCGCAAACTTGGTCGATATCTTATCTGGCCTTTTGGGTTTTCCAATTAGTTCTCTCTCTAAGTTAGAGGTAGGTGCAATGCCTCAAATTTGTAAAAGCTTAGCTAGTCTCGAAGGTGATATAATTCAACCTGGATTCAATAACTGTGAGTTTTTTGAATTGAAACCAACACGTGAGGGATGGCACAACAGAGTCCTCACTTTGTTGAACGGGATTCCTGGAGATGATTTCAAGGACATCCCATTGTTTCTATGTCGGAACTGTACTTTTGAGATTCGGCTTATTCCTTACTACGTCACACCGGATCCGGCAAAGCTTTGCGGGACTTGCAGAAACCCTATGGACAAGGAGGTTCCCTATGATCATGCTAATAGGGATGATGGAGGGATTGTTGCAAGAGGTCGCTTAAGATTCCTCATTTTTGATGACATGAAGATGAAGCCCGCGGGTGATTATGTAGAGCGAGACAGAGAGAGACTGAAGAAAAATGTTGGAGAAGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

207

Amino Acids

23.27

Weight (kDa)

6.33

Isoelectric Point (pI)

30.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 18 - 186 1.7e-11 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 560
AciI CCGC 4 cut(s) 107, 435, 558, 560
AclWI GGATC 2 cut(s) 413, 426
AcsI RAATTY 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 614
AcvI CACGTG 1 cut(s) 287
AfaI GTAC 1 cut(s) 379
AflII CTTAAG 1 cut(s) 520
AflIII ACRYGT 1 cut(s) 284
AgsI TTSAA 8 cut(s) 38, 61, 242, 253, 272, 277, 322, 346
AjnI CCWGG 2 cut(s) 244, 331
AluBI AGCT 3 cut(s) 213, 218, 430
AluI AGCT 3 cut(s) 213, 218, 430
Alw26I GTCTC 3 cut(s) 227, 573, 583
AlwI GGATC 2 cut(s) 413, 426
AoxI GGCC 1 cut(s) 142
ApoI RAATTY 1 cut(s) 201
AspLEI GCGC 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 242, 575
BamHI GGATCC 1 cut(s) 418
BbrPI CACGTG 1 cut(s) 287
BccI CCATC 2 cut(s) 288, 488
BciT130I CCWGG 2 cut(s) 246, 333
BclI TGATCA 1 cut(s) 475
BcoDI GTCTC 3 cut(s) 227, 573, 583
BfaI CTAG 1 cut(s) 219
BfrI CTTAAG 1 cut(s) 520
BlpI GCTNAGC 1 cut(s) 214
Bme1390I CCNGG 2 cut(s) 246, 333
BmiI GGNNCC 2 cut(s) 420, 468
BmrFI CCNGG 2 cut(s) 246, 333
BpmI CTGGAG 1 cut(s) 354
Bpu1102I GCTNAGC 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 558
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bse3DI GCAATG 1 cut(s) 198
BseBI CCWGG 2 cut(s) 246, 333
BseDI CCNNGG 1 cut(s) 558
BseGI GGATG 3 cut(s) 299, 351, 496
BseMI GCAATG 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 560
BshFI GGCC 1 cut(s) 144
BsiSI CCGG 2 cut(s) 416, 422
BslFI GGGAC 1 cut(s) 451
BsmAI GTCTC 3 cut(s) 227, 573, 583
BsmFI GGGAC 1 cut(s) 451
BsnI GGCC 1 cut(s) 144
Bsp143I GATC 3 cut(s) 46, 418, 475
Bsp1720I GCTNAGC 1 cut(s) 214
BspACI CCGC 4 cut(s) 107, 435, 558, 560
BspANI GGCC 1 cut(s) 144
BspFNI CGCG 1 cut(s) 560
BspLI GGNNCC 2 cut(s) 420, 468
BspPI GGATC 2 cut(s) 413, 426
BspTI CTTAAG 1 cut(s) 520
BsrDI GCAATG 1 cut(s) 198
BssECI CCNNGG 1 cut(s) 558
BssMI GATC 3 cut(s) 46, 418, 475
Bst2UI CCWGG 2 cut(s) 246, 333
Bst4CI ACNGT 2 cut(s) 260, 377
BstAFI CTTAAG 1 cut(s) 520
BstBAI YACGTR 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 115, 558
BstDEI CTNAG 2 cut(s) 174, 214
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 3 cut(s) 299, 351, 496
BstFNI CGCG 1 cut(s) 560
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 3 cut(s) 49, 421, 478
BstMAI GTCTC 3 cut(s) 227, 573, 583
BstMBI GATC 3 cut(s) 46, 418, 475
BstMWI GCNNNNNNNGC 1 cut(s) 441
BstNI CCWGG 2 cut(s) 246, 333
BstSCI CCNGG 2 cut(s) 244, 331
BstUI CGCG 1 cut(s) 560
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BsuRI GGCC 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 3 cut(s) 299, 351, 496
Cac8I GCNNGC 2 cut(s) 115, 558
CfoI GCGC 1 cut(s) 119
Cfr42I CCGCGG 1 cut(s) 561
Csp6I GTAC 1 cut(s) 378
CviAII CATG 2 cut(s) 479, 544
CviJI RGCY 6 cut(s) 144, 213, 218, 394, 430, 556
CviKI_1 RGCY 6 cut(s) 144, 213, 218, 394, 430, 556
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 2 cut(s) 174, 214
DpnI GATC 3 cut(s) 48, 420, 477
DpnII GATC 3 cut(s) 46, 418, 475
Eco32I GATATC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 614
Eco72I CACGTG 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 244, 331
EcoRV GATATC 1 cut(s) 133
FaeI CATG 2 cut(s) 482, 547
FaiI YATR 8 cut(s) 78, 236, 367, 455, 474, 480, 545, 570
FaqI GGGAC 1 cut(s) 451
FatI CATG 2 cut(s) 478, 543
FauI CCCGC 3 cut(s) 428, 553, 565
FbaI TGATCA 1 cut(s) 475
FokI GGATG 3 cut(s) 306, 338, 503
FspBI CTAG 1 cut(s) 219
GlaI GCGC 1 cut(s) 118
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 1 cut(s) 144
HapII CCGG 2 cut(s) 416, 422
HhaI GCGC 1 cut(s) 119
Hin1II CATG 2 cut(s) 482, 547
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HindIII AAGCTT 2 cut(s) 211, 428
HinfI GANTC 5 cut(s) 249, 306, 328, 388, 525
HpaII CCGG 2 cut(s) 416, 422
HphI GGTGA 2 cut(s) 242, 575
Hpy188I TCNGA 2 cut(s) 25, 372
Hpy188III TCNNGA 2 cut(s) 85, 224
HpyAV CCTTC 2 cut(s) 221, 608
HpyCH4III ACNGT 2 cut(s) 260, 377
HpyCH4IV ACGT 2 cut(s) 286, 408
HpyCH4V TGCA 3 cut(s) 191, 444, 509
HpyF10VI GCNNNNNNNGC 1 cut(s) 441
HpyF3I CTNAG 2 cut(s) 174, 214
HpySE526I ACGT 2 cut(s) 286, 408
Hsp92II CATG 2 cut(s) 482, 547
HspAI GCGC 1 cut(s) 117
Ksp22I TGATCA 1 cut(s) 475
KspI CCGCGG 1 cut(s) 561
Kzo9I GATC 3 cut(s) 46, 418, 475
LpnPI CCDG 7 cut(s) 126, 231, 258, 318, 345, 429, 435
MaeI CTAG 1 cut(s) 219
MaeII ACGT 2 cut(s) 286, 408
MaeIII GTNAC 1 cut(s) 409
MalI GATC 3 cut(s) 48, 420, 477
MboI GATC 3 cut(s) 46, 418, 475
MboII GAAGA 2 cut(s) 559, 607
MflI RGATCY 1 cut(s) 418
MluCI AATT 5 cut(s) 55, 159, 201, 237, 272
MlyI GAGTC 1 cut(s) 315
MmeI TCCRAC 2 cut(s) 350, 586
MseI TTAA 1 cut(s) 521
MslI CAYNNNNRTG 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 560
MspCI CTTAAG 1 cut(s) 520
MspI CCGG 2 cut(s) 416, 422
MspR9I CCNGG 2 cut(s) 246, 333
MvaI CCWGG 2 cut(s) 246, 333
MvnI CGCG 1 cut(s) 560
MwoI GCNNNNNNNGC 1 cut(s) 441
NdeII GATC 3 cut(s) 46, 418, 475
NlaIII CATG 2 cut(s) 482, 547
NlaIV GGNNCC 2 cut(s) 420, 468
NmuCI GTSAC 1 cut(s) 409
PfeI GAWTC 4 cut(s) 249, 328, 388, 525
PfoI TCCNGGA 1 cut(s) 331
PleI GAGTC 1 cut(s) 314
PmaCI CACGTG 1 cut(s) 287
PmlI CACGTG 1 cut(s) 287
PpsI GAGTC 1 cut(s) 314
Ppu21I YACGTR 1 cut(s) 287
Psp6I CCWGG 2 cut(s) 244, 331
PspCI CACGTG 1 cut(s) 287
PspGI CCWGG 2 cut(s) 244, 331
PspN4I GGNNCC 2 cut(s) 420, 468
PsuI RGATCY 1 cut(s) 418
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
RseI CAYNNNNRTG 1 cut(s) 548
SacII CCGCGG 1 cut(s) 561
SaqAI TTAA 1 cut(s) 521
Sau3AI GATC 3 cut(s) 46, 418, 475
SchI GAGTC 1 cut(s) 315
ScrFI CCNGG 2 cut(s) 246, 333
Sfr303I CCGCGG 1 cut(s) 561
SgrBI CCGCGG 1 cut(s) 561
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 5 cut(s) 55, 159, 201, 237, 272
SsiI CCGC 4 cut(s) 107, 435, 558, 560
SspMI CTAG 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 244, 331
TaaI ACNGT 2 cut(s) 260, 377
TaiI ACGT 2 cut(s) 289, 411
TaqI TCGA 2 cut(s) 129, 225
TasI AATT 5 cut(s) 55, 159, 201, 237, 272
TatI WGTACW 1 cut(s) 377
TfiI GAWTC 4 cut(s) 249, 328, 388, 525
Tru1I TTAA 1 cut(s) 521
Tru9I TTAA 1 cut(s) 521
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 2 cut(s) 560, 566
Vha464I CTTAAG 1 cut(s) 520
XapI RAATTY 1 cut(s) 201
XspI CTAG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.