Rorug01G0316200

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015730
Physical Location & Seq
Reverse (-)
43380670 .. 43381002
333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0316200.1

Sequence Viewer

Length: 333 bp
ATGATTATGGTTACCCACGAGGGTCAAGAGACACAGTTTTCTGCTGAAGATATCTCATCCATGGTTCTTGTAAAGATGCGGGAGATTAATTGCTGGGGTCTTCCTTGGCTCAACTGTGAAGAACGCAGTTATCACCGTCCCAGCTCACTTCGATTAATCACTCACAAAGTCAAGCAAAAGAGAACGCTAGCTGGTGCCATTGCTTCAATCAGTCACAAAGTCAAGCAAAGAAGAACGCTAGCTGGTGCCATTGCTGGCCTAAATGTCATTCATATTCTCAGAGAACCAACAGCCGCAGCCATTGCTTATGGACTCGACAAGAAGGCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

110

Amino Acids

12.25

Weight (kDa)

9.86

Isoelectric Point (pI)

57.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 80 - 108 1.4e-09 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 194, 245
AciI CCGC 2 cut(s) 79, 294
AcuI CTGAAG 1 cut(s) 66
AgsI TTSAA 1 cut(s) 207
AluBI AGCT 3 cut(s) 144, 191, 242
AluI AGCT 3 cut(s) 144, 191, 242
Alw26I GTCTC 1 cut(s) 23
AoxI GGCC 1 cut(s) 256
ApeKI GCWGC 1 cut(s) 296
AseI ATTAAT 2 cut(s) 87, 155
AsuHPI GGTGA 1 cut(s) 125
AsuNHI GCTAGC 2 cut(s) 187, 238
BanI GGYRCC 2 cut(s) 194, 245
BauI CACGAG 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 308
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 2 cut(s) 188, 239
BisI GCNGC 2 cut(s) 294, 297
BlsI GCNGC 2 cut(s) 295, 298
BmiI GGNNCC 2 cut(s) 196, 247
BmsI GCATC 1 cut(s) 66
BmtI GCTAGC 2 cut(s) 191, 242
BpiI GAAGAC 1 cut(s) 92
BsaJI CCNNGG 2 cut(s) 60, 104
Bse3DI GCAATG 3 cut(s) 198, 249, 300
BseDI CCNNGG 2 cut(s) 60, 104
BseGI GGATG 1 cut(s) 56
BseMI GCAATG 3 cut(s) 198, 249, 300
BseMII CTCAG 1 cut(s) 292
BseXI GCAGC 1 cut(s) 308
BseYI CCCAGC 2 cut(s) 93, 140
BshFI GGCC 1 cut(s) 258
BshNI GGYRCC 2 cut(s) 194, 245
BslFI GGGAC 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 1 cut(s) 258
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 2 cut(s) 79, 294
BspANI GGCC 1 cut(s) 258
BspCNI CTCAG 1 cut(s) 291
BspLI GGNNCC 2 cut(s) 196, 247
BspOI GCTAGC 2 cut(s) 191, 242
BspT107I GGYRCC 2 cut(s) 194, 245
BsrDI GCAATG 3 cut(s) 198, 249, 300
BssECI CCNNGG 2 cut(s) 60, 104
BssSI CACGAG 1 cut(s) 17
BssT1I CCWWGG 2 cut(s) 60, 104
Bst2BI CACGAG 1 cut(s) 17
Bst4CI ACNGT 3 cut(s) 36, 116, 137
BstAPI GCANNNNNTGC 1 cut(s) 302
BstC8I GCNNGC 3 cut(s) 189, 240, 256
BstDEI CTNAG 1 cut(s) 278
BstDSI CCRYGG 1 cut(s) 60
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 1 cut(s) 56
BstMAI GTCTC 1 cut(s) 23
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstPI GGTNACC 1 cut(s) 10
BstV1I GCAGC 1 cut(s) 308
BstV2I GAAGAC 1 cut(s) 92
BsuRI GGCC 1 cut(s) 258
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 1 cut(s) 56
Cac8I GCNNGC 3 cut(s) 189, 240, 256
CviAII CATG 1 cut(s) 61
CviJI RGCY 7 cut(s) 109, 144, 191, 242, 258, 293, 299
CviKI_1 RGCY 7 cut(s) 109, 144, 191, 242, 258, 293, 299
DdeI CTNAG 1 cut(s) 278
Eco130I CCWWGG 2 cut(s) 60, 104
Eco32I GATATC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 66
Eco91I GGTNACC 1 cut(s) 10
EcoO65I GGTNACC 1 cut(s) 10
EcoRV GATATC 1 cut(s) 52
EcoT14I CCWWGG 2 cut(s) 60, 104
ErhI CCWWGG 2 cut(s) 60, 104
FaeI CATG 1 cut(s) 64
FaiI YATR 4 cut(s) 8, 62, 273, 309
FaqI GGGAC 1 cut(s) 123
FatI CATG 1 cut(s) 60
FauI CCCGC 1 cut(s) 72
Fnu4HI GCNGC 2 cut(s) 294, 297
FokI GGATG 1 cut(s) 43
Fsp4HI GCNGC 2 cut(s) 294, 297
FspBI CTAG 2 cut(s) 188, 239
GluI GCNGC 2 cut(s) 294, 297
GsaI CCCAGC 2 cut(s) 97, 144
HaeIII GGCC 1 cut(s) 258
Hin1II CATG 1 cut(s) 64
HinfI GANTC 1 cut(s) 312
HphI GGTGA 1 cut(s) 125
Hpy188I TCNGA 1 cut(s) 281
Hpy188III TCNNGA 1 cut(s) 26
HpyAV CCTTC 1 cut(s) 316
HpyCH4III ACNGT 3 cut(s) 36, 116, 137
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 1 cut(s) 278
Hsp92II CATG 1 cut(s) 64
LpnPI CCDG 6 cut(s) 79, 154, 177, 228, 240, 312
Lsp1109I GCAGC 1 cut(s) 308
LweI GCATC 1 cut(s) 66
MaeI CTAG 2 cut(s) 188, 239
MaeIII GTNAC 2 cut(s) 10, 212
MboII GAAGA 4 cut(s) 59, 92, 131, 243
MluCI AATT 1 cut(s) 88
MlyI GAGTC 1 cut(s) 306
MnlI CCTC 1 cut(s) 13
MseI TTAA 2 cut(s) 87, 155
MwoI GCNNNNNNNGC 1 cut(s) 302
NcoI CCATGG 1 cut(s) 60
NheI GCTAGC 2 cut(s) 187, 238
NlaIII CATG 1 cut(s) 64
NlaIV GGNNCC 2 cut(s) 196, 247
NmuCI GTSAC 1 cut(s) 212
PkrI GCNGC 2 cut(s) 295, 298
PleI GAGTC 1 cut(s) 306
PpsI GAGTC 1 cut(s) 306
PshBI ATTAAT 2 cut(s) 87, 155
PspEI GGTNACC 1 cut(s) 10
PspFI CCCAGC 2 cut(s) 93, 140
PspN4I GGNNCC 2 cut(s) 196, 247
SaqAI TTAA 2 cut(s) 87, 155
SatI GCNGC 2 cut(s) 294, 297
SchI GAGTC 1 cut(s) 306
SetI ASST 3 cut(s) 146, 193, 244
SfaNI GCATC 1 cut(s) 66
Sse9I AATT 1 cut(s) 88
SsiI CCGC 2 cut(s) 79, 294
SspMI CTAG 2 cut(s) 188, 239
StyI CCWWGG 2 cut(s) 60, 104
TaaI ACNGT 3 cut(s) 36, 116, 137
TaqI TCGA 2 cut(s) 151, 315
TasI AATT 1 cut(s) 88
TauI GCSGC 1 cut(s) 296
Tru1I TTAA 2 cut(s) 87, 155
Tru9I TTAA 2 cut(s) 87, 155
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 1 cut(s) 296
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 260
VspI ATTAAT 2 cut(s) 87, 155
XspI CTAG 2 cut(s) 188, 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.