RLG00000030795

Mannosylglycoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018161
Physical Location & Seq
Forward (+)
67675673 .. 67676952
1280 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030795

Sequence Viewer

Length: 456 bp
ATGGCTATAGATCGGGCGAGCTCGGGGCTCCAATACCCTGAGCAGTTCTACGTGGCGGTCTCCCATGCCGATTTCGACGGATCTCCTCGCTCCTCCTCTAAAGCCGTTCACGCCAAGTTCAACTCTAAGTCTGCTCTCATCCTCTACGCTCTCTATAAGCAGTCAGGTACTCAGCACCTGGATCTGAATTTCCGTGGAATCAATGACTATGCAGAGGTATATTTAAATGGACACAAAAAGGTCCTTCCAAGAGGGATGTTTCGAAGGCATTCTCTCGATGTCACTGATATAGTAAATGACAATGAAAATGTGCTTGTTGTTGTTGTTTACCCCCTAGATCATTGTGGTTATGTTTTATTTTGTTTAATGCACTTATGGGAGGTTGCCCTAATTGAAAAAAGTGTTGTGGTTGTACTTGGACTAAAATCACTTGAAATCTTCTTAAAATCACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_2 Glyco_hydro_2_C

Protein Analysis

152

Amino Acids

17.03

Weight (kDa)

7.05

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N2 PF22666 54 - 110 2.9e-11 Glycosyl hydrolase 2 galactose-binding domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 56
AclWI GGATC 2 cut(s) 88, 189
AcsI RAATTY 1 cut(s) 187
AfaI GTAC 2 cut(s) 169, 414
AgsI TTSAA 3 cut(s) 121, 395, 434
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 64
AlwI GGATC 2 cut(s) 88, 189
AlwNI CAGNNNCTG 1 cut(s) 178
Ama87I CYCGRG 1 cut(s) 22
ApoI RAATTY 1 cut(s) 187
Asp700I GAANNNNTTC 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 262
AvaI CYCGRG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 241
BanII GRGCYC 2 cut(s) 23, 30
Bbv12I GWGCWC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 6
Bme1390I CCNGG 1 cut(s) 179
Bme18I GGWCC 1 cut(s) 241
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 179
Bpu10I CCTNAGC 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 262
BsaAI YACGTR 1 cut(s) 52
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 1 cut(s) 193
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 2 cut(s) 138, 261
BseMII CTCAG 2 cut(s) 30, 185
BseRI GAGGAG 3 cut(s) 75, 82, 85
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 64
BsmI GAATGC 1 cut(s) 268
Bso31I GGTCTC 1 cut(s) 64
BsoBI CYCGRG 1 cut(s) 22
Bsp119I TTCGAA 1 cut(s) 262
Bsp1286I GDGCHC 2 cut(s) 23, 30
Bsp143I GATC 4 cut(s) 10, 80, 181, 337
BspACI CCGC 1 cut(s) 56
BspCNI CTCAG 2 cut(s) 31, 184
BspLI GGNNCC 1 cut(s) 29
BspPI GGATC 2 cut(s) 88, 189
BspT104I TTCGAA 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 64
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 4 cut(s) 10, 80, 181, 337
Bst2UI CCWGG 1 cut(s) 179
BstBAI YACGTR 1 cut(s) 52
BstBI TTCGAA 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 3 cut(s) 39, 126, 171
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 2 cut(s) 138, 261
BstKTI GATC 4 cut(s) 13, 83, 184, 340
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 4 cut(s) 10, 80, 181, 337
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNI CCWGG 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 177
BstSFI CTRYAG 1 cut(s) 6
BstX2I RGATCY 2 cut(s) 80, 181
BstYI RGATCY 2 cut(s) 80, 181
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 2 cut(s) 138, 261
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 241
Csp6I GTAC 2 cut(s) 168, 413
CviAII CATG 1 cut(s) 65
CviJI RGCY 4 cut(s) 5, 21, 28, 104
CviKI_1 RGCY 4 cut(s) 5, 21, 28, 104
CviQI GTAC 2 cut(s) 168, 413
DdeI CTNAG 3 cut(s) 39, 126, 171
DpnI GATC 4 cut(s) 12, 82, 183, 339
DpnII GATC 4 cut(s) 10, 80, 181, 337
DraI TTTAAA 1 cut(s) 225
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 23, 30
Eco31I GGTCTC 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 177
EcoT38I GRGCYC 2 cut(s) 23, 30
FaeI CATG 1 cut(s) 68
FaiI YATR 8 cut(s) 8, 66, 156, 210, 220, 290, 351, 376
FatI CATG 1 cut(s) 64
FokI GGATG 2 cut(s) 125, 268
FriOI GRGCYC 2 cut(s) 23, 30
FspBI CTAG 1 cut(s) 335
Hin1II CATG 1 cut(s) 68
HinfI GANTC 1 cut(s) 198
Hpy166II GTNNAC 2 cut(s) 109, 328
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 1 cut(s) 275
Hpy8I GTNNAC 2 cut(s) 109, 328
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 254, 258
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 2 cut(s) 212, 370
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 3 cut(s) 39, 126, 171
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 1 cut(s) 68
Kzo9I GATC 4 cut(s) 10, 80, 181, 337
LmnI GCTCC 2 cut(s) 33, 95
LpnPI CCDG 4 cut(s) 51, 150, 164, 191
MaeI CTAG 1 cut(s) 335
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 280
MalI GATC 4 cut(s) 12, 82, 183, 339
MboI GATC 4 cut(s) 10, 80, 181, 337
MboII GAAGA 1 cut(s) 430
MflI RGATCY 2 cut(s) 80, 181
MhlI GDGCHC 2 cut(s) 23, 30
MluCI AATT 2 cut(s) 187, 390
MnlI CCTC 7 cut(s) 96, 103, 106, 152, 208, 245, 373
MroXI GAANNNNTTC 1 cut(s) 268
MseI TTAA 3 cut(s) 224, 365, 443
MspR9I CCNGG 1 cut(s) 179
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 1 cut(s) 179
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeII GATC 4 cut(s) 10, 80, 181, 337
NlaIII CATG 1 cut(s) 68
NlaIV GGNNCC 1 cut(s) 29
NmuCI GTSAC 1 cut(s) 280
NspV TTCGAA 1 cut(s) 262
PctI GAATGC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 198
Ppu21I YACGTR 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 1 cut(s) 241
PspPPI RGGWCCY 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 178
PsuI RGATCY 2 cut(s) 80, 181
RsaI GTAC 2 cut(s) 169, 414
RsaNI GTAC 2 cut(s) 168, 413
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 3 cut(s) 224, 365, 443
Sau3AI GATC 4 cut(s) 10, 80, 181, 337
Sau96I GGNCC 1 cut(s) 241
ScrFI CCNGG 1 cut(s) 179
SduI GDGCHC 2 cut(s) 23, 30
SetI ASST 7 cut(s) 23, 54, 169, 180, 219, 243, 384
SfcI CTRYAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 262
SinI GGWCC 1 cut(s) 241
SmiI ATTTAAAT 1 cut(s) 225
Sse9I AATT 2 cut(s) 187, 390
SsiI CCGC 1 cut(s) 56
SspMI CTAG 1 cut(s) 335
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 177
SwaI ATTTAAAT 1 cut(s) 225
TaiI ACGT 1 cut(s) 54
TaqI TCGA 3 cut(s) 75, 262, 276
TasI AATT 2 cut(s) 187, 390
TatI WGTACW 1 cut(s) 412
TfiI GAWTC 1 cut(s) 198
Tru1I TTAA 3 cut(s) 224, 365, 443
Tru9I TTAA 3 cut(s) 224, 365, 443
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 1 cut(s) 280
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 1 cut(s) 318
TspGWI ACGGA 2 cut(s) 93, 182
TspRI CASTG 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 1 cut(s) 187
XmnI GAANNNNTTC 1 cut(s) 268
XspI CTAG 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.