RLG00000033474

Mannosylglycoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018161
Physical Location & Seq
Forward (+)
27764116 .. 27765451
1336 bp
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UTR
Exon/CDS
Intron
RLM00000033474

Sequence Viewer

Length: 393 bp
ATGGCTATAGATCGGGCGAGCTCGGGGCTCCAATACCCTGAGCAGTTCTACGTGGCGGCCTCCTACGCCGGTTTAAACGGATCTCCTCGCTCCTCCTCTAAAGCCGTTCGCGCCAAGTTCAACTCTGAGTCCGCTCTCATCCTCTACGCTCTCTATAAGCAGTCAGGTACTCAGCACCTAGATCTGAATTTCCGTGGCATCAATTACTATGCAGAGGTATATTTAAATGGGCACAAAAAGGTCCTTCCAAGAGGGATGTTTCAAAGGCATTCTCTAGATGTCACTGATATAGTACATGACAGTGAAAATGTGCTTGCTGTTGTTGTTTACCCCAGATCATTGTGGTTATGTTTTATTTTGTTTAATGCGCTTATGGGAGGTTGTCCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_2 Glyco_hydro_2_C

Protein Analysis

131

Amino Acids

14.52

Weight (kDa)

8.93

Isoelectric Point (pI)

52.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N2 PF22666 54 - 110 2.3e-11 Glycosyl hydrolase 2 galactose-binding domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 134
AccII CGCG 1 cut(s) 111
AciI CCGC 2 cut(s) 56, 132
AclWI GGATC 1 cut(s) 88
AcsI RAATTY 1 cut(s) 187
AfaI GTAC 2 cut(s) 169, 294
AgsI TTSAA 2 cut(s) 121, 263
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 23
AlwI GGATC 1 cut(s) 88
Ama87I CYCGRG 1 cut(s) 22
AoxI GGCC 1 cut(s) 57
ApoI RAATTY 1 cut(s) 187
AspLEI GCGC 2 cut(s) 113, 370
AspS9I GGNCC 1 cut(s) 241
AvaI CYCGRG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 241
BaeGI GKGCMC 1 cut(s) 234
BanII GRGCYC 2 cut(s) 23, 30
Bbv12I GWGCWC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 89
BfaI CTAG 2 cut(s) 179, 275
BfmI CTRYAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 181
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme18I GGWCC 1 cut(s) 241
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 1 cut(s) 29
BmsI GCATC 1 cut(s) 207
Bpu10I CCTNAGC 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 52
BsaJI CCNNGG 1 cut(s) 193
Bse118I RCCGGY 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 2 cut(s) 138, 261
BseMII CTCAG 3 cut(s) 30, 117, 185
BseRI GAGGAG 3 cut(s) 75, 82, 85
BseSI GKGCMC 1 cut(s) 234
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 1 cut(s) 69
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 22
Bsp1286I GDGCHC 3 cut(s) 23, 30, 234
Bsp143I GATC 4 cut(s) 10, 80, 181, 335
BspACI CCGC 2 cut(s) 56, 132
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 3 cut(s) 31, 118, 184
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 29
BspPI GGATC 1 cut(s) 88
BsrBI CCGCTC 1 cut(s) 134
BsrFI RCCGGY 1 cut(s) 68
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 4 cut(s) 10, 80, 181, 335
Bst4CI ACNGT 1 cut(s) 302
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 2 cut(s) 19, 315
BstDEI CTNAG 3 cut(s) 39, 126, 171
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 2 cut(s) 138, 261
BstFNI CGCG 1 cut(s) 111
BstHHI GCGC 2 cut(s) 113, 370
BstKTI GATC 4 cut(s) 13, 83, 184, 338
BstMBI GATC 4 cut(s) 10, 80, 181, 335
BstMWI GCNNNNNNNGC 2 cut(s) 65, 110
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 1 cut(s) 111
BstX2I RGATCY 2 cut(s) 80, 181
BstYI RGATCY 2 cut(s) 80, 181
BsuRI GGCC 1 cut(s) 59
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 2 cut(s) 138, 261
BtsIMutI CAGTG 2 cut(s) 282, 307
Cac8I GCNNGC 2 cut(s) 19, 315
CfoI GCGC 2 cut(s) 113, 370
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 241
Csp6I GTAC 2 cut(s) 168, 293
CviAII CATG 1 cut(s) 296
CviJI RGCY 5 cut(s) 5, 21, 28, 59, 104
CviKI_1 RGCY 5 cut(s) 5, 21, 28, 59, 104
CviQI GTAC 2 cut(s) 168, 293
DdeI CTNAG 3 cut(s) 39, 126, 171
DpnI GATC 4 cut(s) 12, 82, 183, 337
DpnII GATC 4 cut(s) 10, 80, 181, 335
DraI TTTAAA 2 cut(s) 75, 225
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 23, 30
Eco47I GGWCC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 241
EcoT38I GRGCYC 2 cut(s) 23, 30
FaeI CATG 1 cut(s) 299
FaiI YATR 8 cut(s) 8, 156, 210, 220, 290, 297, 349, 374
FatI CATG 1 cut(s) 295
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 2 cut(s) 125, 268
FriOI GRGCYC 2 cut(s) 23, 30
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 2 cut(s) 179, 275
GlaI GCGC 2 cut(s) 112, 369
GluI GCNGC 1 cut(s) 57
HaeIII GGCC 1 cut(s) 59
HapII CCGG 1 cut(s) 69
HhaI GCGC 2 cut(s) 113, 370
Hin1II CATG 1 cut(s) 299
Hin6I GCGC 2 cut(s) 111, 368
HinP1I GCGC 2 cut(s) 111, 368
HinfI GANTC 1 cut(s) 128
HpaII CCGG 1 cut(s) 69
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 2 cut(s) 127, 186
Hpy188III TCNNGA 1 cut(s) 275
Hpy8I GTNNAC 1 cut(s) 328
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 110
HpyF3I CTNAG 3 cut(s) 39, 126, 171
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 1 cut(s) 299
HspAI GCGC 2 cut(s) 111, 368
Kzo9I GATC 4 cut(s) 10, 80, 181, 335
LmnI GCTCC 2 cut(s) 33, 95
LpnPI CCDG 4 cut(s) 51, 82, 150, 346
LweI GCATC 1 cut(s) 207
MaeI CTAG 2 cut(s) 179, 275
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 280
MalI GATC 4 cut(s) 12, 82, 183, 337
MbiI CCGCTC 1 cut(s) 134
MboI GATC 4 cut(s) 10, 80, 181, 335
MflI RGATCY 2 cut(s) 80, 181
MhlI GDGCHC 3 cut(s) 23, 30, 234
MluCI AATT 3 cut(s) 187, 202, 388
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 8 cut(s) 70, 96, 103, 106, 152, 208, 245, 371
MseI TTAA 3 cut(s) 74, 224, 363
MslI CAYNNNNRTG 1 cut(s) 300
MspI CCGG 1 cut(s) 69
MssI GTTTAAAC 1 cut(s) 75
Mva1269I GAATGC 1 cut(s) 268
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 65, 110
NdeII GATC 4 cut(s) 10, 80, 181, 335
NlaIII CATG 1 cut(s) 299
NlaIV GGNNCC 1 cut(s) 29
NmuCI GTSAC 1 cut(s) 280
PctI GAATGC 1 cut(s) 268
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 1 cut(s) 136
PmeI GTTTAAAC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 136
Ppu21I YACGTR 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 1 cut(s) 241
PspPPI RGGWCCY 1 cut(s) 241
PsuI RGATCY 2 cut(s) 80, 181
RsaI GTAC 2 cut(s) 169, 294
RsaNI GTAC 2 cut(s) 168, 293
RseI CAYNNNNRTG 1 cut(s) 300
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 3 cut(s) 74, 224, 363
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 4 cut(s) 10, 80, 181, 335
Sau96I GGNCC 1 cut(s) 241
SchI GAGTC 1 cut(s) 137
SduI GDGCHC 3 cut(s) 23, 30, 234
SetI ASST 7 cut(s) 23, 54, 169, 180, 219, 243, 382
SfaNI GCATC 1 cut(s) 207
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 241
SmiI ATTTAAAT 1 cut(s) 225
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 3 cut(s) 187, 202, 388
SsiI CCGC 2 cut(s) 56, 132
SspMI CTAG 2 cut(s) 179, 275
SstI GAGCTC 1 cut(s) 23
SwaI ATTTAAAT 1 cut(s) 225
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 1 cut(s) 54
TasI AATT 3 cut(s) 187, 202, 388
TatI WGTACW 1 cut(s) 292
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 3 cut(s) 74, 224, 363
Tru9I TTAA 3 cut(s) 74, 224, 363
TscAI CASTG 2 cut(s) 289, 307
TseFI GTSAC 1 cut(s) 280
Tsp45I GTSAC 1 cut(s) 280
TspGWI ACGGA 2 cut(s) 93, 182
TspRI CASTG 2 cut(s) 289, 307
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 1 cut(s) 187
XbaI TCTAGA 1 cut(s) 274
XspI CTAG 2 cut(s) 179, 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.