Rh2AG380100

Mannosylglycoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018161
Physical Location & Seq
Reverse (-)
56983199 .. 56986222
3024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG380100.1

Sequence Viewer

Length: 471 bp
ATGGCTATAGATCGGGCGAGCTCGGGGCTCCAATACCCTGAGCGGTTCTACGTGGCGGCCTCCTGCGCCGGTTTCGACGGATCTCCTCGCTCCTCCTCTAAAGCCGTTCGCGCCAAGTTCAACTCTGAGTCCGCTCTCATCCTCGACGCTCTCTATAAGCAGTCAGGTACTCAGCACCTAGATCTGAATTTCCGTGGAATCAATTACTATGCAGAGGTATATTTAAATGGGCACAAAAAGGTCCTTCCAAGAGGGATGTTTCGAAGGCATTCTCTAGATGTCACTGATATAGTACTTGACAGTGAAAATAATTTAAATTTTGGAATTGACTTAGACAACAGCATTGCAAGTAAGGATGCTTCTCCATTTGAACTTTTAGTTTTTCTATTTGAAAATATCATTATTTCATTTGAAGTTCTGGATGATGTTATGTGTAATTGCAAGTCCCCATGGAATAGATATCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_2 Glyco_hydro_2_C

Protein Analysis

156

Amino Acids

17.65

Weight (kDa)

5.56

Isoelectric Point (pI)

48.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N2 PF22666 53 - 110 9.3e-11 Glycosyl hydrolase 2 galactose-binding domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 43, 134
AccII CGCG 1 cut(s) 111
AciI CCGC 3 cut(s) 43, 56, 132
AclWI GGATC 1 cut(s) 88
AcsI RAATTY 2 cut(s) 187, 316
AfaI GTAC 2 cut(s) 169, 294
AgsI TTSAA 4 cut(s) 121, 371, 392, 413
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 23
AlwI GGATC 1 cut(s) 88
Ama87I CYCGRG 1 cut(s) 22
AoxI GGCC 1 cut(s) 57
ApoI RAATTY 2 cut(s) 187, 316
Asp700I GAANNNNTTC 1 cut(s) 268
AspLEI GCGC 2 cut(s) 68, 113
AspS9I GGNCC 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 262
AvaI CYCGRG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 241
BaeGI GKGCMC 1 cut(s) 234
BanII GRGCYC 2 cut(s) 23, 30
BarI GAAGNNNNNNTAC 2 cut(s) 343, 375
Bbv12I GWGCWC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 89
BfaI CTAG 2 cut(s) 179, 275
BfmI CTRYAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 181
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
BmcAI AGTACT 1 cut(s) 294
Bme18I GGWCC 1 cut(s) 241
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 1 cut(s) 29
BmsI GCATC 1 cut(s) 346
Bpu10I CCTNAGC 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 262
BsaAI YACGTR 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 193, 449
Bse118I RCCGGY 1 cut(s) 68
Bse3DI GCAATG 1 cut(s) 342
BseDI CCNNGG 2 cut(s) 193, 449
BseGI GGATG 4 cut(s) 138, 261, 361, 427
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 3 cut(s) 30, 117, 185
BseRI GAGGAG 3 cut(s) 75, 82, 85
BseSI GKGCMC 1 cut(s) 234
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 1 cut(s) 430
BsmFI GGGAC 1 cut(s) 430
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 22
Bsp119I TTCGAA 1 cut(s) 262
Bsp1286I GDGCHC 3 cut(s) 23, 30, 234
Bsp143I GATC 3 cut(s) 10, 80, 181
Bsp19I CCATGG 1 cut(s) 449
BspACI CCGC 3 cut(s) 43, 56, 132
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 3 cut(s) 31, 118, 184
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 29
BspPI GGATC 1 cut(s) 88
BspT104I TTCGAA 1 cut(s) 262
BsrBI CCGCTC 2 cut(s) 43, 134
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 68
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 193, 449
BssMI GATC 3 cut(s) 10, 80, 181
BssT1I CCWWGG 1 cut(s) 449
Bst4CI ACNGT 1 cut(s) 302
BstBAI YACGTR 1 cut(s) 52
BstBI TTCGAA 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 4 cut(s) 39, 126, 171, 331
BstDSI CCRYGG 2 cut(s) 193, 449
BstF5I GGATG 4 cut(s) 138, 261, 361, 427
BstFNI CGCG 1 cut(s) 111
BstHHI GCGC 2 cut(s) 68, 113
BstKTI GATC 3 cut(s) 13, 83, 184
BstMBI GATC 3 cut(s) 10, 80, 181
BstMWI GCNNNNNNNGC 2 cut(s) 65, 110
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 1 cut(s) 111
BstX2I RGATCY 2 cut(s) 80, 181
BstYI RGATCY 2 cut(s) 80, 181
BsuRI GGCC 1 cut(s) 59
BtgI CCRYGG 2 cut(s) 193, 449
BtsCI GGATG 4 cut(s) 138, 261, 361, 427
BtsIMutI CAGTG 2 cut(s) 282, 307
Cac8I GCNNGC 1 cut(s) 19
CfoI GCGC 2 cut(s) 68, 113
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 241
CseI GACGC 1 cut(s) 155
Csp6I GTAC 2 cut(s) 168, 293
CviAII CATG 1 cut(s) 450
CviJI RGCY 5 cut(s) 5, 21, 28, 59, 104
CviKI_1 RGCY 5 cut(s) 5, 21, 28, 59, 104
CviQI GTAC 2 cut(s) 168, 293
DdeI CTNAG 4 cut(s) 39, 126, 171, 331
DpnI GATC 3 cut(s) 12, 82, 183
DpnII GATC 3 cut(s) 10, 80, 181
DraI TTTAAA 2 cut(s) 225, 315
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 449
Eco24I GRGCYC 2 cut(s) 23, 30
Eco32I GATATC 1 cut(s) 461
Eco47I GGWCC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 241
EcoRV GATATC 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 449
EcoT38I GRGCYC 2 cut(s) 23, 30
ErhI CCWWGG 1 cut(s) 449
FaeI CATG 1 cut(s) 453
FaiI YATR 7 cut(s) 8, 156, 210, 220, 290, 431, 451
FaqI GGGAC 1 cut(s) 430
FatI CATG 1 cut(s) 449
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 4 cut(s) 125, 268, 368, 434
FriOI GRGCYC 2 cut(s) 23, 30
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 2 cut(s) 179, 275
GlaI GCGC 2 cut(s) 67, 112
GluI GCNGC 1 cut(s) 57
HaeIII GGCC 1 cut(s) 59
HapII CCGG 1 cut(s) 69
HgaI GACGC 1 cut(s) 155
HhaI GCGC 2 cut(s) 68, 113
Hin1II CATG 1 cut(s) 453
Hin6I GCGC 2 cut(s) 66, 111
HinP1I GCGC 2 cut(s) 66, 111
HinfI GANTC 2 cut(s) 128, 198
HpaII CCGG 1 cut(s) 69
Hpy188I TCNGA 2 cut(s) 127, 186
Hpy188III TCNNGA 2 cut(s) 275, 419
Hpy99I CGWCG 2 cut(s) 80, 149
HpyAV CCTTC 2 cut(s) 254, 258
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 3 cut(s) 212, 347, 441
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 110
HpyF3I CTNAG 4 cut(s) 39, 126, 171, 331
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 1 cut(s) 453
HspAI GCGC 2 cut(s) 66, 111
Kzo9I GATC 3 cut(s) 10, 80, 181
LmnI GCTCC 2 cut(s) 33, 95
LpnPI CCDG 5 cut(s) 51, 76, 82, 150, 404
LweI GCATC 1 cut(s) 346
MaeI CTAG 2 cut(s) 179, 275
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 280
MalI GATC 3 cut(s) 12, 82, 183
MbiI CCGCTC 2 cut(s) 43, 134
MboI GATC 3 cut(s) 10, 80, 181
MflI RGATCY 2 cut(s) 80, 181
MhlI GDGCHC 3 cut(s) 23, 30, 234
MluCI AATT 6 cut(s) 187, 202, 310, 316, 324, 436
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 7 cut(s) 70, 96, 103, 106, 152, 208, 245
MroXI GAANNNNTTC 1 cut(s) 268
MseI TTAA 2 cut(s) 224, 314
MspI CCGG 1 cut(s) 69
Mva1269I GAATGC 1 cut(s) 268
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 65, 110
NcoI CCATGG 1 cut(s) 449
NdeII GATC 3 cut(s) 10, 80, 181
NlaIII CATG 1 cut(s) 453
NlaIV GGNNCC 1 cut(s) 29
NmuCI GTSAC 1 cut(s) 280
NspV TTCGAA 1 cut(s) 262
PctI GAATGC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 198
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
Ppu21I YACGTR 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 1 cut(s) 241
PspPPI RGGWCCY 1 cut(s) 241
PsuI RGATCY 2 cut(s) 80, 181
RsaI GTAC 2 cut(s) 169, 294
RsaNI GTAC 2 cut(s) 168, 293
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 224, 314
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 3 cut(s) 10, 80, 181
Sau96I GGNCC 1 cut(s) 241
ScaI AGTACT 1 cut(s) 294
SchI GAGTC 1 cut(s) 137
SduI GDGCHC 3 cut(s) 23, 30, 234
SetI ASST 6 cut(s) 23, 54, 169, 180, 219, 243
SfaNI GCATC 1 cut(s) 346
SfcI CTRYAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 262
SinI GGWCC 1 cut(s) 241
SmiI ATTTAAAT 2 cut(s) 225, 315
Sse9I AATT 6 cut(s) 187, 202, 310, 316, 324, 436
SsiI CCGC 3 cut(s) 43, 56, 132
SspI AATATT 1 cut(s) 467
SspMI CTAG 2 cut(s) 179, 275
SstI GAGCTC 1 cut(s) 23
StyI CCWWGG 1 cut(s) 449
SwaI ATTTAAAT 2 cut(s) 225, 315
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 1 cut(s) 54
TaqI TCGA 3 cut(s) 75, 144, 262
TasI AATT 6 cut(s) 187, 202, 310, 316, 324, 436
TatI WGTACW 1 cut(s) 292
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 1 cut(s) 198
Tru1I TTAA 2 cut(s) 224, 314
Tru9I TTAA 2 cut(s) 224, 314
TscAI CASTG 2 cut(s) 289, 307
TseFI GTSAC 1 cut(s) 280
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 1 cut(s) 396
TspGWI ACGGA 2 cut(s) 93, 182
TspRI CASTG 2 cut(s) 289, 307
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 2 cut(s) 187, 316
XbaI TCTAGA 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 268
XspI CTAG 2 cut(s) 179, 275
ZrmI AGTACT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.