Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0011291

Physical Location & Seq

Genomic Coordinates Forward (+)

13860292 .. 13865832

Gene Structure 5541 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032350

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGGAATTGTCAAATGGGCAAGTGGGGTTCTCTAATTTTCAAAGAAATGGGATTTTGGCTACGAATTGTAGCACAAAGCCATTTCCTTTATCTGGGTTTTCGATTTTTGGGATACCCATTTTCAGCATTGGCTTATGTGAGAAGAATGTGAAGAAGAGCCGGGTTTCTGGTATTAAAACAGTGAATCGTGGAAGTGTTATTAGTGCTTTGGCAAAGGAAGGGACTGATAATCGGTCAGTTGGTAGTGAAATTCTGGAGAAAGATTTTGAATTCAAGCCATCGTTTGATCAATGCTTGAAGGTTATGGGGTCTGTTAAGCTTAGAAGTGACAAAGACAAGAAGAGCCGGCCAAAAGAAGATAGTCCAAAGCATAATATGAGGGGTAGGGATGTTTCCAGAAGACAGTTGTCTGAGGCAAATGAAGAGCGTGTAAAATCGGGGGAGCCTGAAACGCATTTGGATCGAGGGAATGTGTCAAAAGTTGCGAAACTATATGAAAATGCTAATGGAACTGCAGAACAAAGCAAAAGGCTACGAGTTAGAGGATATAAGGATGAATATGATAGCAGGCAGAGTGACATGGATGAAAAGGAAAAGATGAAGGTAAGGGGAGATACAAGTGATGGAAAATGGTCGAGATATACTGGTAGACCAGAACAAGAATTGGACTTCAACTCAGGTAAGGGTGCATTGGCACGAAATGCTAAAGAAAGCCCAAGAGATTACAAGTCAACTGGTCAAGACTTTGAGAGAAGGAAAAGTGGTGTTAGAAGTGAAGATGGATTTGAGAGGTACCGCATCAGTGCTGAGAAAGCCACTGATAATAAAGCTTTCTCTCCTAGAAGTGGAGATTCAACCAAGAACGGAAGAGATTTCCCTAGGAGAGACTATGATGAAAGAGCTGCATTTAAGAATTTTGATGAATTCAGTGATATTATGGACAAGCCACGAGTTTCACAAATGCAAATGGAGGAGAGAATCCGGAAGCTAGCAAAATCGCTGAATGGTGCAAACATCGACATGCCTGAGTGGATGTTTTCAAAGATGATGCGAAGTGCACAAATCAGATTCACAGATCATTCAATACTTAGAGTTATTCAGATATTAGGAAAATTCGGAAACTGGAGACGAGTGTTACAAGTCATTGAGTGGCTTCAAATGCGTGAGCGCTTCAAATCTCACAAGCTCAGATACATATATACAACTGCTCTCAATGTACTTGGAAAGGCAAGGAGACCTGTGGAGGCACTAAATGTATTTCATGCAATGCTGCAACAACTATCATCATATCCTGACTTGGTAGCTTATCATTCCATTGCCATTACTCTTGGACAAGCGGGCCATATGAAAGAACTGTTTGATGTGATTGATACTATGCGGTCTCCTCCCAAGAAGAAGTTCAAGACTGGAGCACTTGGGAAGTGGGATCCTCGGTTGGAACCAGATGCCATTGTCTACAATGCAGTACTAAATGCTTGTGTTCAGCGGAAACAATGGGAAGGAGCATTTTGGGTTTTGGAGCAGTTAAAGAAACAAGGTGTGCAGCCTGCCACTACAACATATGGACTTGTCATGGAGGTGATGTTTGCATGTGGCAAGTACAATTTGGTTCACGAGTTTTTTAAGAAGATGCAGAAATCTTCTATACCAAATGCTTTAACTTACAGAGTTATTGTGAATACTCTTTGGAGAGAAGATAAAATAGATGAGGCTGTACAGACTGTTCACAATATGGAAAGACGAGGAATAGTAGGTTCTGCAGCTCTTTATTATGACTTCGCTCGATGTCTTTGTAGTGCTGGAAGGTGTCAAGAAGCACTAATGCAGATTGAGAAGATATGTAAAGTTGCAAATAAGCCTTTAGTAGTGACTTACACTGGTTTAATTCAAGCTTGTTTGGACGCTGGAAGCATTGAAAATGGGGCATATGTCTTCAACCAAATGGAGAACTTCTGTTCCCCAAATCTGGTCACTTGCAACATAATGCTGAAAGGCTACTTGGACCATAGGATGTATGAAGAAGCAAAACAGCTGTTCCAAAAGATGTTGGAAGATGGGTACAATATCAGCAGCAAATCTGGTTATGAGCTGCGGGTGACACCAGATATCTATACATTCAACACTTTGTTAGAAGCATGCATCACAGAGAAAAGGTGGGATGATTTCGAGTCTTTCTACAAAAGGATGCTGCAAAGTGGATATAACTTCAATATTAAACGACATCTTCGGATGATATTGGATGCTTGCAAAGCAGGAAAGGGTGAGCTACTGGATATAACCTGGGTGCACTTGACCGAGGCAGACCGAATTCCACCCCCTGCTCTCATCAAGGAAAGGTTTTGCAAAAAGCTGGAGGAAGATAACTATTCTGTTGCTATATCCTGCATTGCCAGAACGAATCCCGATGATCTGCAGGCATTCTCAAAGACAGCGTGGTTGAATTTGTTCAAGGAAAATGCAGAAAGGTTTCAGAAAGACACTCTTCTGCAGTTAATTCGTGAGGTTAGCATTCTTACTGCCAAAAGTGATGCGGCGAACCCTGTATTTGAAAACCTTGTGACGTGTTGTAGAGAACTTGATAGAACTGGTGTGAGGACAGGTGACTTGAAACCAAATGAAAGTGTATGTACTGTTCAAACTGAACCAGCTTATTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

886

Amino Acids

101.29

Weight (kDa)

9.15

Isoelectric Point (pI)

40.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	479 - 527	2.2e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	481 - 523	1.3e-07	PPR repeat family
PPR_2	PF13041	654 - 690	6e-07	PPR repeat family
PPR	PF01535	657 - 686	1.3e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30610 AT1G30610
fragaria_vesca	FvH4_3g11350
malus_domestica	MD05G1264100.v1.1 MD10G1242100.v1.1
prunus_persica	Prupe.4G101500_v2.0.a1
pyrus_communis	pycom05g24820
rosa_chinensis	RchiOBHm_Chr5g0018311
rosa_laevigata	RLG00000032350
rosa_multiflora	Rmu_sc0007362.1_g000034 Rmu_sc0011919.1_g000006 Rmu_sc0038151.1_g000001 Rmu_sc0038657.1_g000001
rosa_roxburghii	Rroxscaffold_1G00059430
rosa_rugosa	Rorug05G0041300
rosa_samantha	Rh5AG134400 Rh5BG133200 Rh5CG144900 Rh5DG134600
rosa_wichuraiana	Rw5G011750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	792
AccB1I	GGYRCC	1 cut(s)	792
AccI	GTMKAC	2 cut(s)	649, 1455
AccIII	TCCGGA	1 cut(s)	981
AciI	CCGC	6 cut(s)	796, 1337, 1378, 1486, 2092, 2531
AclWI	GGATC	3 cut(s)	468, 1421, 1434
AcoI	YGGCCR	1 cut(s)	347
AcsI	RAATTY	7 cut(s)	249, 269, 913, 923, 1112, 2307, 2440
AfaI	GTAC	7 cut(s)	794, 1218, 1467, 1601, 1716, 2060, 2629
AfeI	AGCGCT	1 cut(s)	1169
AfiI	CCNNNNNNNGG	3 cut(s)	92, 845, 1966
AflIII	ACRYGT	1 cut(s)	2561
AjiI	CACGTC	1 cut(s)	2562
AjnI	CCWGG	1 cut(s)	2279
AjuI	GAANNNNNNNTTGG	4 cut(s)	1382, 1414, 1982, 2014
AloI	GAACNNNNNNTCC	2 cut(s)	1940, 1972
Alw21I	GWGCWC	3 cut(s)	1060, 1414, 2289
Alw26I	GTCTC	4 cut(s)	879, 1120, 1228, 1386
Alw44I	GTGCAC	2 cut(s)	1056, 2285
AlwI	GGATC	3 cut(s)	468, 1421, 1434
Aor13HI	TCCGGA	1 cut(s)	981
Aor51HI	AGCGCT	1 cut(s)	1169
AoxI	GGCC	2 cut(s)	347, 1339
ApaLI	GTGCAC	2 cut(s)	1056, 2285
ApeKI	GCWGC	7 cut(s)	902, 1270, 1543, 1760, 2070, 2089, 2188
ApoI	RAATTY	7 cut(s)	249, 269, 913, 923, 1112, 2307, 2440
Asp700I	GAANNNNTTC	3 cut(s)	1397, 2444, 2466
Asp718I	GGTACC	1 cut(s)	792
AspA2I	CCTAGG	1 cut(s)	878
AspLEI	GCGC	1 cut(s)	1170
AspS9I	GGNCC	2 cut(s)	1339, 2002
AsuC2I	CCSGG	1 cut(s)	161
AsuHPI	GGTGA	4 cut(s)	1591, 2107, 2273, 2612
AsuNHI	GCTAGC	1 cut(s)	988
AvaII	GGWCC	1 cut(s)	2002
AvrII	CCTAGG	1 cut(s)	878
BaeGI	GKGCMC	2 cut(s)	1060, 2289
BamHI	GGATCC	1 cut(s)	1426
BanI	GGYRCC	1 cut(s)	792
BauI	CACGAG	2 cut(s)	948, 1613
BbsI	GAAGAC	2 cut(s)	406, 1924
Bbv12I	GWGCWC	3 cut(s)	1060, 1414, 2289
BbvI	GCAGC	7 cut(s)	889, 1257, 1555, 1772, 2076, 2082, 2175
BccI	CCATC	4 cut(s)	286, 617, 773, 2048
BcgI	CGANNNNNNTGC	4 cut(s)	443, 477, 2477, 2511
BciT130I	CCWGG	1 cut(s)	2281
BciVI	GTATCC	1 cut(s)	105
BclI	TGATCA	1 cut(s)	286
BcnI	CCSGG	1 cut(s)	161
BcoDI	GTCTC	4 cut(s)	879, 1120, 1228, 1386
BfaI	CTAG	3 cut(s)	840, 879, 989
BfmI	CTRYAG	4 cut(s)	513, 1758, 2411, 2486
BfoI	RGCGCY	1 cut(s)	1171
BfuI	GTATCC	1 cut(s)	105
BisI	GCNGC	8 cut(s)	903, 1271, 1544, 1761, 2071, 2090, 2189, 2532
BlnI	CCTAGG	1 cut(s)	878
BlsI	GCNGC	8 cut(s)	904, 1272, 1545, 1762, 2072, 2091, 2190, 2533
BmcAI	AGTACT	1 cut(s)	1467
Bme1390I	CCNGG	2 cut(s)	161, 2281
Bme18I	GGWCC	1 cut(s)	2002
BmgBI	CACGTC	1 cut(s)	2562
BmgT120I	GGNCC	2 cut(s)	1339, 2002
BmiI	GGNNCC	4 cut(s)	444, 794, 1428, 1440
BmrFI	CCNGG	2 cut(s)	161, 2281
BmsI	GCATC	8 cut(s)	807, 1038, 1435, 1620, 2148, 2175, 2230, 2518
BmtI	GCTAGC	1 cut(s)	992
BoxI	GACNNNNGTC	1 cut(s)	406
BpiI	GAAGAC	2 cut(s)	406, 1924
BpmI	CTGGAG	4 cut(s)	275, 1144, 1428, 2372
BpuMI	CCSGG	1 cut(s)	161
BsaI	GGTCTC	2 cut(s)	1228, 1386
BsaJI	CCNNGG	4 cut(s)	878, 1430, 2280, 2295
BsaWI	WCCGGW	1 cut(s)	981
BsaXI	ACNNNNNCTCC	2 cut(s)	1568, 1598
Bsc4I	CCNNNNNNNGG	3 cut(s)	92, 845, 1966
Bse118I	RCCGGY	1 cut(s)	345
Bse1I	ACTGG	7 cut(s)	649, 739, 1127, 1411, 1882, 2274, 2590
Bse3DI	GCAATG	3 cut(s)	1272, 1314, 2385
BseAI	TCCGGA	1 cut(s)	981
BseBI	CCWGG	1 cut(s)	2281
BseDI	CCNNGG	4 cut(s)	878, 1430, 2280, 2295
BseGI	GGATG	9 cut(s)	394, 559, 589, 1038, 2016, 2164, 2190, 2235, 2245
BseLI	CCNNNNNNNGG	3 cut(s)	92, 845, 1966
BseMI	GCAATG	3 cut(s)	1272, 1314, 2385
BseMII	CTCAG	5 cut(s)	402, 690, 798, 1017, 1201
BseNI	ACTGG	7 cut(s)	649, 739, 1127, 1411, 1882, 2274, 2590
BseRI	GAGGAG	2 cut(s)	986, 1374
BseSI	GKGCMC	2 cut(s)	1060, 2289
BseXI	GCAGC	7 cut(s)	889, 1257, 1555, 1772, 2076, 2082, 2175
BsgI	GTGCAG	1 cut(s)	1562
BshFI	GGCC	2 cut(s)	349, 1341
BshNI	GGYRCC	1 cut(s)	792
BsiHKAI	GWGCWC	3 cut(s)	1060, 1414, 2289
BsiSI	CCGG	3 cut(s)	160, 346, 982
BslFI	GGGAC	1 cut(s)	235
BslI	CCNNNNNNNGG	3 cut(s)	92, 845, 1966
BsmAI	GTCTC	4 cut(s)	879, 1120, 1228, 1386
BsmBI	CGTCTC	1 cut(s)	1120
BsmFI	GGGAC	1 cut(s)	235
BsmI	GAATGC	2 cut(s)	2417, 2508
BsnI	GGCC	2 cut(s)	349, 1341
Bso31I	GGTCTC	2 cut(s)	1228, 1386
Bsp1286I	GDGCHC	3 cut(s)	1060, 1414, 2289
Bsp13I	TCCGGA	1 cut(s)	981
Bsp1407I	TGTACA	1 cut(s)	1714
Bsp143I	GATC	5 cut(s)	286, 460, 1075, 1426, 2407
BspACI	CCGC	6 cut(s)	796, 1337, 1378, 1486, 2092, 2531
BspANI	GGCC	2 cut(s)	349, 1341
BspCNI	CTCAG	5 cut(s)	403, 689, 799, 1018, 1200
BspEI	TCCGGA	1 cut(s)	981
BspLI	GGNNCC	4 cut(s)	444, 794, 1428, 1440
BspMAI	CTGCAG	4 cut(s)	517, 1762, 2415, 2490
BspOI	GCTAGC	1 cut(s)	992
BspPI	GGATC	3 cut(s)	468, 1421, 1434
BspQI	GCTCTTC	3 cut(s)	149, 335, 417
BspT107I	GGYRCC	1 cut(s)	792
BspTNI	GGTCTC	2 cut(s)	1228, 1386
BsrDI	GCAATG	3 cut(s)	1272, 1314, 2385
BsrFI	RCCGGY	1 cut(s)	345
BsrGI	TGTACA	1 cut(s)	1714
BsrI	ACTGG	7 cut(s)	649, 739, 1127, 1411, 1882, 2274, 2590
BssAI	RCCGGY	1 cut(s)	345
BssECI	CCNNGG	4 cut(s)	878, 1430, 2280, 2295
BssMI	GATC	5 cut(s)	286, 460, 1075, 1426, 2407
BssSI	CACGAG	2 cut(s)	948, 1613
BssT1I	CCWWGG	1 cut(s)	878
Bst2BI	CACGAG	2 cut(s)	948, 1613
Bst2UI	CCWGG	1 cut(s)	2281
Bst4CI	ACNGT	5 cut(s)	181, 405, 1356, 1723, 2632
Bst6I	CTCTTC	5 cut(s)	149, 335, 417, 862, 2487
BstAPI	GCANNNNNTGC	1 cut(s)	701
BstAUI	TGTACA	1 cut(s)	1714
BstC8I	GCNNGC	8 cut(s)	347, 569, 990, 1339, 1548, 2137, 2245, 2415
BstDEI	CTNAG	7 cut(s)	320, 411, 676, 807, 1026, 1088, 1187
BstF5I	GGATG	9 cut(s)	394, 559, 589, 1038, 2016, 2164, 2190, 2235, 2245
BstH2I	RGCGCY	1 cut(s)	1171
BstHHI	GCGC	1 cut(s)	1170
BstKTI	GATC	5 cut(s)	289, 463, 1078, 1429, 2410
BstMAI	GTCTC	4 cut(s)	879, 1120, 1228, 1386
BstMBI	GATC	5 cut(s)	286, 460, 1075, 1426, 2407
BstMWI	GCNNNNNNNGC	5 cut(s)	451, 701, 812, 1159, 2249
BstNI	CCWGG	1 cut(s)	2281
BstNSI	RCATGY	3 cut(s)	1024, 1593, 2139
BstPAI	GACNNNNGTC	1 cut(s)	406
BstSCI	CCNGG	2 cut(s)	159, 2279
BstSFI	CTRYAG	4 cut(s)	513, 1758, 2411, 2486
BstSLI	GKGCMC	2 cut(s)	1060, 2289
BstV1I	GCAGC	7 cut(s)	889, 1257, 1555, 1772, 2076, 2082, 2175
BstV2I	GAAGAC	2 cut(s)	406, 1924
BstX2I	RGATCY	1 cut(s)	1426
BstYI	RGATCY	1 cut(s)	1426
BsuI	GTATCC	1 cut(s)	105
BsuRI	GGCC	2 cut(s)	349, 1341
BtrI	CACGTC	1 cut(s)	2562
BtsCI	GGATG	9 cut(s)	394, 559, 589, 1038, 2016, 2164, 2190, 2235, 2245
BtsIMutI	CAGTG	5 cut(s)	186, 808, 816, 934, 1875
Cac8I	GCNNGC	8 cut(s)	347, 569, 990, 1339, 1548, 2137, 2245, 2415
CfoI	GCGC	1 cut(s)	1170
Cfr10I	RCCGGY	1 cut(s)	345
Cfr13I	GGNCC	2 cut(s)	1339, 2002
CseI	GACGC	1 cut(s)	1910
Csp6I	GTAC	7 cut(s)	793, 1217, 1466, 1600, 1715, 2059, 2628
CviAII	CATG	6 cut(s)	580, 1021, 1262, 1573, 1590, 2136
CviQI	GTAC	7 cut(s)	793, 1217, 1466, 1600, 1715, 2059, 2628
DdeI	CTNAG	7 cut(s)	320, 411, 676, 807, 1026, 1088, 1187
DpnI	GATC	5 cut(s)	288, 462, 1077, 1428, 2409
DpnII	GATC	5 cut(s)	286, 460, 1075, 1426, 2407
EaeI	YGGCCR	1 cut(s)	347
Eam1104I	CTCTTC	5 cut(s)	149, 335, 417, 862, 2487
EarI	CTCTTC	5 cut(s)	149, 335, 417, 862, 2487
Eco130I	CCWWGG	1 cut(s)	878
Eco31I	GGTCTC	2 cut(s)	1228, 1386
Eco32I	GATATC	1 cut(s)	2107
Eco47I	GGWCC	1 cut(s)	2002
Eco47III	AGCGCT	1 cut(s)	1169
EcoRI	GAATTC	3 cut(s)	269, 923, 2307
EcoRII	CCWGG	1 cut(s)	2279
EcoRV	GATATC	1 cut(s)	2107
EcoT14I	CCWWGG	1 cut(s)	878
EcoT22I	ATGCAT	1 cut(s)	2141
ErhI	CCWWGG	1 cut(s)	878
Esp3I	CGTCTC	1 cut(s)	1120
FaeI	CATG	6 cut(s)	583, 1024, 1265, 1576, 1593, 2139
FalI	AAGNNNNNCTT	2 cut(s)	2466, 2498
FaqI	GGGAC	1 cut(s)	235
FatI	CATG	6 cut(s)	579, 1020, 1261, 1572, 1589, 2135
FauI	CCCGC	2 cut(s)	1330, 2085
FauNDI	CATATG	3 cut(s)	1344, 1561, 1927
FbaI	TGATCA	1 cut(s)	286
FblI	GTMKAC	2 cut(s)	649, 1455
Fnu4HI	GCNGC	8 cut(s)	903, 1271, 1544, 1761, 2071, 2090, 2189, 2532
FokI	GGATG	9 cut(s)	401, 566, 596, 1045, 2023, 2171, 2197, 2242, 2252
Fsp4HI	GCNGC	8 cut(s)	903, 1271, 1544, 1761, 2071, 2090, 2189, 2532
FspBI	CTAG	3 cut(s)	840, 879, 989
GlaI	GCGC	1 cut(s)	1169
GluI	GCNGC	8 cut(s)	903, 1271, 1544, 1761, 2071, 2090, 2189, 2532
GsuI	CTGGAG	4 cut(s)	275, 1144, 1428, 2372
HaeII	RGCGCY	1 cut(s)	1171
HaeIII	GGCC	2 cut(s)	349, 1341
HapII	CCGG	3 cut(s)	160, 346, 982
HgaI	GACGC	1 cut(s)	1910
HhaI	GCGC	1 cut(s)	1170
Hin1II	CATG	6 cut(s)	583, 1024, 1265, 1576, 1593, 2139
Hin6I	GCGC	1 cut(s)	1168
HinP1I	GCGC	1 cut(s)	1168
HincII	GTYRAC	1 cut(s)	732
HindII	GTYRAC	1 cut(s)	732
HindIII	AAGCTT	3 cut(s)	317, 828, 1890
HinfI	GANTC	6 cut(s)	184, 851, 978, 1068, 2168, 2398
HpaII	CCGG	3 cut(s)	160, 346, 982
HphI	GGTGA	4 cut(s)	1591, 2107, 2273, 2612
Hpy166II	GTNNAC	7 cut(s)	650, 732, 1058, 1456, 1612, 1726, 2287
Hpy188I	TCNGA	7 cut(s)	412, 1067, 1101, 1118, 1190, 2229, 2472
Hpy8I	GTNNAC	7 cut(s)	650, 732, 1058, 1456, 1612, 1726, 2287
HpyAV	CCTTC	6 cut(s)	212, 292, 595, 747, 1493, 1797
HpyCH4III	ACNGT	5 cut(s)	181, 405, 1356, 1723, 2632
HpyCH4IV	ACGT	1 cut(s)	2561
HpyF10VI	GCNNNNNNNGC	5 cut(s)	451, 701, 812, 1159, 2249
HpyF3I	CTNAG	7 cut(s)	320, 411, 676, 807, 1026, 1088, 1187
HpySE526I	ACGT	1 cut(s)	2561
Hsp92II	CATG	6 cut(s)	583, 1024, 1265, 1576, 1593, 2139
HspAI	GCGC	1 cut(s)	1168
Kpn2I	TCCGGA	1 cut(s)	981
KpnI	GGTACC	1 cut(s)	796
KroI	GCCGGC	1 cut(s)	345
KroNI	GCCGGC	1 cut(s)	347
Ksp22I	TGATCA	1 cut(s)	286
Kzo9I	GATC	5 cut(s)	286, 460, 1075, 1426, 2407
LguI	GCTCTTC	3 cut(s)	149, 335, 417
LmnI	GCTCC	4 cut(s)	442, 1409, 1502, 1519
Lsp1109I	GCAGC	7 cut(s)	889, 1257, 1555, 1772, 2076, 2082, 2175
LweI	GCATC	8 cut(s)	807, 1038, 1435, 1620, 2148, 2175, 2230, 2518
MaeI	CTAG	3 cut(s)	840, 879, 989
MaeII	ACGT	1 cut(s)	2561
MaeIII	GTNAC	8 cut(s)	326, 575, 1134, 1867, 1969, 2095, 2557, 2600
MalI	GATC	5 cut(s)	288, 462, 1077, 1428, 2409
MboI	GATC	5 cut(s)	286, 460, 1075, 1426, 2407
MflI	RGATCY	1 cut(s)	1426
MhlI	GDGCHC	3 cut(s)	1060, 1414, 2289
MlyI	GAGTC	1 cut(s)	2177
MmeI	TCCRAC	2 cut(s)	1416, 2028
Mph1103I	ATGCAT	1 cut(s)	2141
MroI	TCCGGA	1 cut(s)	981
MroNI	GCCGGC	1 cut(s)	345
MroXI	GAANNNNTTC	3 cut(s)	1397, 2444, 2466
MseI	TTAA	9 cut(s)	174, 315, 909, 1526, 1623, 1658, 1883, 2214, 2492
MslI	CAYNNNNRTG	2 cut(s)	1019, 1577
MspA1I	CMGCKG	2 cut(s)	1486, 2032
MspI	CCGG	3 cut(s)	160, 346, 982
MspR9I	CCNGG	2 cut(s)	161, 2281
Mva1269I	GAATGC	2 cut(s)	2417, 2508
MvaI	CCWGG	1 cut(s)	2281
MwoI	GCNNNNNNNGC	5 cut(s)	451, 701, 812, 1159, 2249
NaeI	GCCGGC	1 cut(s)	347
NciI	CCSGG	1 cut(s)	161
NdeI	CATATG	3 cut(s)	1344, 1561, 1927
NdeII	GATC	5 cut(s)	286, 460, 1075, 1426, 2407
NgoMIV	GCCGGC	1 cut(s)	345
NheI	GCTAGC	1 cut(s)	988
NlaIII	CATG	6 cut(s)	583, 1024, 1265, 1576, 1593, 2139
NlaIV	GGNNCC	4 cut(s)	444, 794, 1428, 1440
NmuCI	GTSAC	7 cut(s)	326, 575, 1867, 1969, 2095, 2557, 2600
NsiI	ATGCAT	1 cut(s)	2141
NspI	RCATGY	3 cut(s)	1024, 1593, 2139
PaeI	GCATGC	1 cut(s)	2139
PciSI	GCTCTTC	3 cut(s)	149, 335, 417
PctI	GAATGC	2 cut(s)	2417, 2508
PdiI	GCCGGC	1 cut(s)	347
PdmI	GAANNNNTTC	3 cut(s)	1397, 2444, 2466
PfeI	GAWTC	5 cut(s)	184, 851, 978, 1068, 2398
PkrI	GCNGC	8 cut(s)	904, 1272, 1545, 1762, 2072, 2091, 2190, 2533
PleI	GAGTC	1 cut(s)	2176
PpsI	GAGTC	1 cut(s)	2176
PshAI	GACNNNNGTC	1 cut(s)	406
Psp6I	CCWGG	1 cut(s)	2279
PspGI	CCWGG	1 cut(s)	2279
PspN4I	GGNNCC	4 cut(s)	444, 794, 1428, 1440
PspPI	GGNCC	2 cut(s)	1339, 2002
PsrI	GAACNNNNNNTAC	4 cut(s)	1707, 1739, 2616, 2648
PstI	CTGCAG	4 cut(s)	517, 1762, 2415, 2490
PsuI	RGATCY	1 cut(s)	1426
PvuII	CAGCTG	1 cut(s)	2032
RsaI	GTAC	7 cut(s)	794, 1218, 1467, 1601, 1716, 2060, 2629
RsaNI	GTAC	7 cut(s)	793, 1217, 1466, 1600, 1715, 2059, 2628
RseI	CAYNNNNRTG	2 cut(s)	1019, 1577
SapI	GCTCTTC	3 cut(s)	149, 335, 417
SaqAI	TTAA	9 cut(s)	174, 315, 909, 1526, 1623, 1658, 1883, 2214, 2492
SatI	GCNGC	8 cut(s)	903, 1271, 1544, 1761, 2071, 2090, 2189, 2532
Sau3AI	GATC	5 cut(s)	286, 460, 1075, 1426, 2407
Sau96I	GGNCC	2 cut(s)	1339, 2002
ScaI	AGTACT	1 cut(s)	1467
SchI	GAGTC	1 cut(s)	2177
ScrFI	CCNGG	2 cut(s)	161, 2281
SduI	GDGCHC	3 cut(s)	1060, 1414, 2289
SfaNI	GCATC	8 cut(s)	807, 1038, 1435, 1620, 2148, 2175, 2230, 2518
SfcI	CTRYAG	4 cut(s)	513, 1758, 2411, 2486
SinI	GGWCC	1 cut(s)	2002
SmiMI	CAYNNNNRTG	2 cut(s)	1019, 1577
SphI	GCATGC	1 cut(s)	2139
SsiI	CCGC	6 cut(s)	796, 1337, 1378, 1486, 2092, 2531
SspI	AATATT	1 cut(s)	2212
SspMI	CTAG	3 cut(s)	840, 879, 989
StyD4I	CCNGG	2 cut(s)	159, 2279
StyI	CCWWGG	1 cut(s)	878
TaaI	ACNGT	5 cut(s)	181, 405, 1356, 1723, 2632
TaiI	ACGT	1 cut(s)	2564
TaqI	TCGA	6 cut(s)	101, 463, 635, 1017, 1783, 2166
TaqII	GACCGA	3 cut(s)	222, 2309, 2319
TatI	WGTACW	5 cut(s)	1216, 1465, 1599, 1714, 2627
TauI	GCSGC	1 cut(s)	2534
TfiI	GAWTC	5 cut(s)	184, 851, 978, 1068, 2398
Tru1I	TTAA	9 cut(s)	174, 315, 909, 1526, 1623, 1658, 1883, 2214, 2492
Tru9I	TTAA	9 cut(s)	174, 315, 909, 1526, 1623, 1658, 1883, 2214, 2492
TscAI	CASTG	5 cut(s)	186, 808, 823, 934, 1882
TseFI	GTSAC	7 cut(s)	326, 575, 1867, 1969, 2095, 2557, 2600
TseI	GCWGC	7 cut(s)	902, 1270, 1543, 1760, 2070, 2089, 2188
Tsp45I	GTSAC	7 cut(s)	326, 575, 1867, 1969, 2095, 2557, 2600
TspGWI	ACGGA	1 cut(s)	879
TspRI	CASTG	5 cut(s)	186, 808, 823, 934, 1882
VneI	GTGCAC	2 cut(s)	1056, 2285
VpaK11BI	GGWCC	1 cut(s)	2002
XapI	RAATTY	7 cut(s)	249, 269, 913, 923, 1112, 2307, 2440
XceI	RCATGY	3 cut(s)	1024, 1593, 2139
XmaJI	CCTAGG	1 cut(s)	878
XmiI	GTMKAC	2 cut(s)	649, 1455
XmnI	GAANNNNTTC	3 cut(s)	1397, 2444, 2466
XspI	CTAG	3 cut(s)	840, 879, 989
ZrmI	AGTACT	1 cut(s)	1467
Zsp2I	ATGCAT	1 cut(s)	2141

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.