RLG00000032578

Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011972
Physical Location & Seq
Forward (+)
16706555 .. 16707980
1426 bp
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UTR
Exon/CDS
Intron
RLM00000032578

Sequence Viewer

Length: 318 bp
ATGGCCGGAGTAATAACAAAGTTCGTTACTGCATCATTGTTTATGTGGACAGTTCCTGTTGCAATATTGTATGGGTATAACAACAATTTGATTCCTGGTTCAAGCGACTTGTCTCCCCATACTCTCACTCTGTTGAGTGGATTCCTTGCTGTTGTTTCTGTCAATATAGTTATTGCATTCTATATATATCTGGCAATGAAGGAACCTTCGGATAAACATGAGCCTGATCCTGCATTTCTTGCTGAGGCCAAAGCTAGTGTAAACCAGTCGACAGCTGACGCTGGTAGCTCTTCCCAATCCTCCAAGAAAGACGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VMA21

Protein Analysis

106

Amino Acids

11.2

Weight (kDa)

5.17

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VMA21 PF09446 4 - 66 2.7e-20 VMA21-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05780 AT2G31710
fragaria_vesca FvH4_3g12930 FvH4_4g01060
malus_domestica MD05G1247100.v1.1 MD10G1227700.v1.1
prunus_persica Prupe.4G117300_v2.0.a1 Prupe.4G117300_v2.0.a1
pyrus_communis pycom05g22300 pycom10g19120
rosa_chinensis RchiOBHm_Chr5g0021041
rosa_laevigata RLG00000032578
rosa_multiflora Rmu_co8487873.1_g000001
rosa_rugosa Rorug05G0063800
rosa_samantha Rh5AG153400 Rh5BG153100 Rh5CG165900 Rh5DG152700
rosa_wichuraiana Rw5G013630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 269
AclWI GGATC 1 cut(s) 221
AcoI YGGCCR 1 cut(s) 3
AgsI TTSAA 1 cut(s) 102
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 3 cut(s) 254, 275, 288
AluI AGCT 3 cut(s) 254, 275, 288
Alw26I GTCTC 1 cut(s) 117
AlwI GGATC 1 cut(s) 221
AlwNI CAGNNNCTG 1 cut(s) 56
AoxI GGCC 2 cut(s) 3, 246
BbvCI CCTCAGC 1 cut(s) 243
BciT130I CCWGG 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 1 cut(s) 255
Bme1390I CCNGG 1 cut(s) 96
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 96
BmsI GCATC 1 cut(s) 41
Bpu10I CCTNAGC 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 1 cut(s) 96
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 265
BshFI GGCC 2 cut(s) 5, 248
BsiSI CCGG 1 cut(s) 6
BsmAI GTCTC 1 cut(s) 117
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 2 cut(s) 5, 248
Bsp143I GATC 1 cut(s) 226
BspANI GGCC 2 cut(s) 5, 248
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 1 cut(s) 204
BspPI GGATC 1 cut(s) 221
BspQI GCTCTTC 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 201
BsrI ACTGG 1 cut(s) 265
BssMI GATC 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 52
Bst6I CTCTTC 1 cut(s) 295
BstAPI GCANNNNNTGC 1 cut(s) 239
BstDEI CTNAG 1 cut(s) 243
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 94
BsuRI GGCC 2 cut(s) 5, 248
CaiI CAGNNNCTG 1 cut(s) 56
CseI GACGC 1 cut(s) 287
CviAII CATG 1 cut(s) 218
CviJI RGCY 6 cut(s) 5, 223, 248, 254, 275, 288
CviKI_1 RGCY 6 cut(s) 5, 223, 248, 254, 275, 288
DdeI CTNAG 1 cut(s) 243
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 94
FaeI CATG 1 cut(s) 221
FaiI YATR 9 cut(s) 44, 72, 78, 120, 167, 183, 185, 187, 219
FatI CATG 1 cut(s) 217
FblI GTMKAC 1 cut(s) 269
FspBI CTAG 1 cut(s) 255
HaeIII GGCC 2 cut(s) 5, 248
HapII CCGG 1 cut(s) 6
HgaI GACGC 1 cut(s) 287
Hin1II CATG 1 cut(s) 221
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HinfI GANTC 2 cut(s) 91, 141
HpaII CCGG 1 cut(s) 6
Hpy166II GTNNAC 3 cut(s) 48, 262, 270
Hpy188I TCNGA 1 cut(s) 211
Hpy8I GTNNAC 3 cut(s) 48, 262, 270
HpyAV CCTTC 2 cut(s) 193, 216
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4V TGCA 4 cut(s) 32, 62, 176, 233
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 1 cut(s) 243
Hsp92II CATG 1 cut(s) 221
Kzo9I GATC 1 cut(s) 226
LguI GCTCTTC 1 cut(s) 295
LpnPI CCDG 9 cut(s) 19, 69, 81, 108, 176, 237, 243, 267, 278
LweI GCATC 1 cut(s) 41
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 25
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 1 cut(s) 282
MluCI AATT 1 cut(s) 85
MnlI CCTC 2 cut(s) 238, 310
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 96
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 96
MwoI GCNNNNNNNGC 1 cut(s) 239
NdeII GATC 1 cut(s) 226
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 1 cut(s) 204
PciSI GCTCTTC 1 cut(s) 295
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 91, 141
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 204
PstNI CAGNNNCTG 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 275
SalI GTCGAC 1 cut(s) 268
SapI GCTCTTC 1 cut(s) 295
Sau3AI GATC 1 cut(s) 226
ScrFI CCNGG 1 cut(s) 96
SetI ASST 4 cut(s) 208, 256, 277, 290
SfaNI GCATC 1 cut(s) 41
Sse9I AATT 1 cut(s) 85
SspI AATATT 1 cut(s) 66
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 52
TaqI TCGA 1 cut(s) 269
TasI AATT 1 cut(s) 85
TfiI GAWTC 2 cut(s) 91, 141
TspDTI ATGAA 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 269
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.