Rh5AG153400

Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011972
Physical Location & Seq
Forward (+)
16168206 .. 16170577
2372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG153400.1

Sequence Viewer

Length: 369 bp
ATGTTGATATTGATTATTTTTCTTGTTTATTCTATAGATTGGAGATATCAAATGGCCGGAGTAATAACTAAGTTTGTTACTGCATCATTGTTTATGTGGACAGTTCCTGTTGCAATATTGTATGGGTATAACAACAATTTGATTCCTGGTTCGAGTGACTTGTCTCCCCATACTCTCACTCTGTTGAGTGGATTCCTTGCTGTTGTTTCTGTCAATATAGTTATTGCATTCTATATATATATGGCAATGAAGGAACCTTCAGATAAACATGAGCCTGATCCTGCATTTCTTGCTGAGGCCAAAGCTAGTGTAAACCAGTCTACAGCTGACACGGGCAGCTCTTCCCAATCCTCCAAGAAAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VMA21

Protein Analysis

122

Amino Acids

13.43

Weight (kDa)

5.21

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VMA21 PF09446 21 - 83 7.6e-21 VMA21-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05780 AT2G31710
fragaria_vesca FvH4_3g12930 FvH4_4g01060
malus_domestica MD05G1247100.v1.1 MD10G1227700.v1.1
prunus_persica Prupe.4G117300_v2.0.a1 Prupe.4G117300_v2.0.a1
pyrus_communis pycom05g22300 pycom10g19120
rosa_chinensis RchiOBHm_Chr5g0021041
rosa_laevigata RLG00000032578
rosa_multiflora Rmu_co8487873.1_g000001
rosa_rugosa Rorug05G0063800
rosa_samantha Rh5AG153400 Rh5BG153100 Rh5CG165900 Rh5DG152700
rosa_wichuraiana Rw5G013630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 320
AclWI GGATC 1 cut(s) 272
AcoI YGGCCR 1 cut(s) 54
AcuI CTGAAG 1 cut(s) 243
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 3 cut(s) 305, 326, 339
AluI AGCT 3 cut(s) 305, 326, 339
Alw26I GTCTC 1 cut(s) 168
AlwI GGATC 1 cut(s) 272
AlwNI CAGNNNCTG 1 cut(s) 107
AoxI GGCC 2 cut(s) 54, 297
ApeKI GCWGC 1 cut(s) 336
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 348
BciT130I CCWGG 1 cut(s) 147
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 1 cut(s) 306
BfmI CTRYAG 2 cut(s) 33, 321
BisI GCNGC 1 cut(s) 337
BlsI GCNGC 1 cut(s) 338
Bme1390I CCNGG 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 92
Bpu10I CCTNAGC 1 cut(s) 294
Bse1I ACTGG 1 cut(s) 316
Bse3DI GCAATG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 147
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 316
BseXI GCAGC 1 cut(s) 348
BshFI GGCC 2 cut(s) 56, 299
BsiSI CCGG 1 cut(s) 57
BsmAI GTCTC 1 cut(s) 168
BsmI GAATGC 1 cut(s) 227
BsnI GGCC 2 cut(s) 56, 299
Bsp143I GATC 1 cut(s) 277
BspANI GGCC 2 cut(s) 56, 299
BspCNI CTCAG 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 255
BspPI GGATC 1 cut(s) 272
BspQI GCTCTTC 1 cut(s) 346
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 1 cut(s) 316
BssMI GATC 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 2 cut(s) 346, 357
BstAPI GCANNNNNTGC 1 cut(s) 290
BstDEI CTNAG 2 cut(s) 69, 294
BstKTI GATC 1 cut(s) 280
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 1 cut(s) 277
BstMWI GCNNNNNNNGC 1 cut(s) 290
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 2 cut(s) 33, 321
BstV1I GCAGC 1 cut(s) 348
BsuRI GGCC 2 cut(s) 56, 299
CaiI CAGNNNCTG 1 cut(s) 107
CviAII CATG 1 cut(s) 269
CviJI RGCY 6 cut(s) 56, 274, 299, 305, 326, 339
CviKI_1 RGCY 6 cut(s) 56, 274, 299, 305, 326, 339
DdeI CTNAG 2 cut(s) 69, 294
DpnI GATC 1 cut(s) 279
DpnII GATC 1 cut(s) 277
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 2 cut(s) 346, 357
EarI CTCTTC 2 cut(s) 346, 357
Eco32I GATATC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 145
EcoRV GATATC 1 cut(s) 47
FaeI CATG 1 cut(s) 272
FatI CATG 1 cut(s) 268
FblI GTMKAC 1 cut(s) 320
Fnu4HI GCNGC 1 cut(s) 337
Fsp4HI GCNGC 1 cut(s) 337
FspBI CTAG 1 cut(s) 306
GluI GCNGC 1 cut(s) 337
HaeIII GGCC 2 cut(s) 56, 299
HapII CCGG 1 cut(s) 57
Hin1II CATG 1 cut(s) 272
HinfI GANTC 2 cut(s) 142, 192
HpaII CCGG 1 cut(s) 57
Hpy166II GTNNAC 3 cut(s) 99, 313, 321
Hpy188I TCNGA 1 cut(s) 262
Hpy8I GTNNAC 3 cut(s) 99, 313, 321
HpyAV CCTTC 2 cut(s) 244, 267
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4V TGCA 4 cut(s) 83, 113, 227, 284
HpyF10VI GCNNNNNNNGC 1 cut(s) 290
HpyF3I CTNAG 2 cut(s) 69, 294
Hsp92II CATG 1 cut(s) 272
Kzo9I GATC 1 cut(s) 277
LguI GCTCTTC 1 cut(s) 346
LpnPI CCDG 7 cut(s) 70, 120, 132, 159, 288, 294, 329
Lsp1109I GCAGC 1 cut(s) 348
LweI GCATC 1 cut(s) 92
MaeI CTAG 1 cut(s) 306
MaeIII GTNAC 2 cut(s) 76, 155
MalI GATC 1 cut(s) 279
MboI GATC 1 cut(s) 277
MboII GAAGA 1 cut(s) 333
MluCI AATT 1 cut(s) 136
MnlI CCTC 2 cut(s) 289, 361
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 1 cut(s) 147
Mva1269I GAATGC 1 cut(s) 227
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 290
NdeII GATC 1 cut(s) 277
NlaIII CATG 1 cut(s) 272
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 1 cut(s) 155
PciSI GCTCTTC 1 cut(s) 346
PctI GAATGC 1 cut(s) 227
PfeI GAWTC 2 cut(s) 142, 192
PkrI GCNGC 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 255
PstNI CAGNNNCTG 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 346
SatI GCNGC 1 cut(s) 337
Sau3AI GATC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 147
SetI ASST 4 cut(s) 259, 307, 328, 341
SfaNI GCATC 1 cut(s) 92
SfcI CTRYAG 2 cut(s) 33, 321
Sse9I AATT 1 cut(s) 136
SspI AATATT 1 cut(s) 117
SspMI CTAG 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 1 cut(s) 103
TaqI TCGA 1 cut(s) 152
TasI AATT 1 cut(s) 136
TfiI GAWTC 2 cut(s) 142, 192
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 1 cut(s) 336
Tsp45I GTSAC 1 cut(s) 155
TspDTI ATGAA 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.