Rh5DG152700

Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011972
Physical Location & Seq
Forward (+)
15523490 .. 15526077
2588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG152700.1

Sequence Viewer

Length: 408 bp
ATGCCAAAATTCTCTCCTTCTGGATTCGGCCTCTCAACTCCAGCGACTCAATCTCCGGATCCAGCACTCTCGCCTGGATTGGAGATATCAATGGCCGGAGTAATTACAAAGTTCGTTACTGCGTCATTGTTTATGTGGACAGTTCCTGTTGCAATATTGTATGGGTATAACAACAATTTGATTCCTGGTTCGAGTGACTTGTCTCCCCATACTCTCACTCTGTTGAGTGGATTCCTTGCTGTTGTTTCTGTCAATATAGTTATTGCATTCTATATATATATGGCAATGAAGGAACCTTCAGATAAACATGAGCCTGATCCTGCATTTCTTGCTGAGGCCAAAGCTAGTGTAAACCAGTCTACAGCTGACACGGGCAGCTCTTCCCAATCCTCCAAGAAAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VMA21

Protein Analysis

135

Amino Acids

14.26

Weight (kDa)

5.0

Isoelectric Point (pI)

62.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VMA21 PF09446 34 - 96 9.8e-21 VMA21-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05780 AT2G31710
fragaria_vesca FvH4_3g12930 FvH4_4g01060
malus_domestica MD05G1247100.v1.1 MD10G1227700.v1.1
prunus_persica Prupe.4G117300_v2.0.a1 Prupe.4G117300_v2.0.a1
pyrus_communis pycom05g22300 pycom10g19120
rosa_chinensis RchiOBHm_Chr5g0021041
rosa_laevigata RLG00000032578
rosa_multiflora Rmu_co8487873.1_g000001
rosa_rugosa Rorug05G0063800
rosa_samantha Rh5AG153400 Rh5BG153100 Rh5CG165900 Rh5DG152700
rosa_wichuraiana Rw5G013630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 359
AccIII TCCGGA 1 cut(s) 55
AclWI GGATC 3 cut(s) 53, 66, 311
AcoI YGGCCR 1 cut(s) 93
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 282
AjnI CCWGG 2 cut(s) 73, 184
AluBI AGCT 3 cut(s) 344, 365, 378
AluI AGCT 3 cut(s) 344, 365, 378
Alw26I GTCTC 1 cut(s) 207
AlwI GGATC 3 cut(s) 53, 66, 311
AlwNI CAGNNNCTG 1 cut(s) 146
Aor13HI TCCGGA 1 cut(s) 55
AoxI GGCC 3 cut(s) 28, 93, 336
ApeKI GCWGC 1 cut(s) 375
ApoI RAATTY 1 cut(s) 8
BamHI GGATCC 1 cut(s) 58
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 1 cut(s) 387
BciT130I CCWGG 2 cut(s) 75, 186
BcoDI GTCTC 1 cut(s) 207
BfaI CTAG 1 cut(s) 345
BfmI CTRYAG 1 cut(s) 360
BisI GCNGC 1 cut(s) 376
BlsI GCNGC 1 cut(s) 377
Bme1390I CCNGG 2 cut(s) 75, 186
BmiI GGNNCC 2 cut(s) 60, 294
BmrFI CCNGG 2 cut(s) 75, 186
BpmI CTGGAG 1 cut(s) 24
Bpu10I CCTNAGC 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 37, 67
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 291
BseAI TCCGGA 1 cut(s) 55
BseBI CCWGG 2 cut(s) 75, 186
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 355
BseXI GCAGC 1 cut(s) 387
BshFI GGCC 3 cut(s) 30, 95, 338
BsiSI CCGG 2 cut(s) 56, 96
BsmAI GTCTC 1 cut(s) 207
BsmI GAATGC 1 cut(s) 266
BsnI GGCC 3 cut(s) 30, 95, 338
Bsp13I TCCGGA 1 cut(s) 55
Bsp143I GATC 2 cut(s) 58, 316
BspANI GGCC 3 cut(s) 30, 95, 338
BspCNI CTCAG 1 cut(s) 325
BspEI TCCGGA 1 cut(s) 55
BspLI GGNNCC 2 cut(s) 60, 294
BspPI GGATC 3 cut(s) 53, 66, 311
BspQI GCTCTTC 1 cut(s) 385
BsrDI GCAATG 1 cut(s) 291
BsrI ACTGG 1 cut(s) 355
BssMI GATC 2 cut(s) 58, 316
Bst2UI CCWGG 2 cut(s) 75, 186
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 2 cut(s) 385, 396
BstAPI GCANNNNNTGC 1 cut(s) 329
BstDEI CTNAG 1 cut(s) 333
BstKTI GATC 2 cut(s) 61, 319
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 2 cut(s) 58, 316
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 2 cut(s) 75, 186
BstSCI CCNGG 2 cut(s) 73, 184
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BsuRI GGCC 3 cut(s) 30, 95, 338
CaiI CAGNNNCTG 1 cut(s) 146
CseI GACGC 1 cut(s) 111
CviAII CATG 1 cut(s) 308
CviJI RGCY 7 cut(s) 30, 95, 313, 338, 344, 365, 378
CviKI_1 RGCY 7 cut(s) 30, 95, 313, 338, 344, 365, 378
DdeI CTNAG 1 cut(s) 333
DpnI GATC 2 cut(s) 60, 318
DpnII GATC 2 cut(s) 58, 316
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 2 cut(s) 385, 396
EarI CTCTTC 2 cut(s) 385, 396
Eco32I GATATC 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 282
EcoRII CCWGG 2 cut(s) 73, 184
EcoRV GATATC 1 cut(s) 87
FaeI CATG 1 cut(s) 311
FatI CATG 1 cut(s) 307
FblI GTMKAC 1 cut(s) 359
Fnu4HI GCNGC 1 cut(s) 376
Fsp4HI GCNGC 1 cut(s) 376
FspBI CTAG 1 cut(s) 345
GluI GCNGC 1 cut(s) 376
GsuI CTGGAG 1 cut(s) 24
HaeIII GGCC 3 cut(s) 30, 95, 338
HapII CCGG 2 cut(s) 56, 96
HgaI GACGC 1 cut(s) 111
Hin1II CATG 1 cut(s) 311
HinfI GANTC 4 cut(s) 24, 46, 181, 231
HpaII CCGG 2 cut(s) 56, 96
Hpy166II GTNNAC 3 cut(s) 138, 352, 360
Hpy188I TCNGA 1 cut(s) 301
Hpy188III TCNNGA 2 cut(s) 21, 56
Hpy8I GTNNAC 3 cut(s) 138, 352, 360
HpyAV CCTTC 3 cut(s) 27, 283, 306
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4V TGCA 3 cut(s) 152, 266, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 1 cut(s) 333
Hsp92II CATG 1 cut(s) 311
Kpn2I TCCGGA 1 cut(s) 55
Kzo9I GATC 2 cut(s) 58, 316
LguI GCTCTTC 1 cut(s) 385
Lsp1109I GCAGC 1 cut(s) 387
MaeI CTAG 1 cut(s) 345
MaeIII GTNAC 2 cut(s) 115, 194
MalI GATC 2 cut(s) 60, 318
MboI GATC 2 cut(s) 58, 316
MboII GAAGA 1 cut(s) 372
MflI RGATCY 1 cut(s) 58
MluCI AATT 3 cut(s) 8, 102, 175
MlyI GAGTC 1 cut(s) 40
MnlI CCTC 3 cut(s) 41, 328, 400
MroI TCCGGA 1 cut(s) 55
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 2 cut(s) 56, 96
MspR9I CCNGG 2 cut(s) 75, 186
Mva1269I GAATGC 1 cut(s) 266
MvaI CCWGG 2 cut(s) 75, 186
MwoI GCNNNNNNNGC 1 cut(s) 329
NdeII GATC 2 cut(s) 58, 316
NlaIII CATG 1 cut(s) 311
NlaIV GGNNCC 2 cut(s) 60, 294
NmuCI GTSAC 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 385
PctI GAATGC 1 cut(s) 266
PfeI GAWTC 3 cut(s) 24, 181, 231
PkrI GCNGC 1 cut(s) 377
PleI GAGTC 1 cut(s) 40
PpsI GAGTC 1 cut(s) 40
Psp6I CCWGG 2 cut(s) 73, 184
PspGI CCWGG 2 cut(s) 73, 184
PspN4I GGNNCC 2 cut(s) 60, 294
PstNI CAGNNNCTG 1 cut(s) 146
PsuI RGATCY 1 cut(s) 58
PvuII CAGCTG 1 cut(s) 365
SapI GCTCTTC 1 cut(s) 385
SatI GCNGC 1 cut(s) 376
Sau3AI GATC 2 cut(s) 58, 316
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 2 cut(s) 75, 186
SetI ASST 4 cut(s) 298, 346, 367, 380
SfcI CTRYAG 1 cut(s) 360
Sse9I AATT 3 cut(s) 8, 102, 175
SspI AATATT 1 cut(s) 156
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 2 cut(s) 73, 184
TaaI ACNGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 191
TasI AATT 3 cut(s) 8, 102, 175
TfiI GAWTC 3 cut(s) 24, 181, 231
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 1 cut(s) 375
Tsp45I GTSAC 1 cut(s) 194
TspDTI ATGAA 1 cut(s) 302
XapI RAATTY 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 359
XspI CTAG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.