Rh5BG153100

Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011972
Physical Location & Seq
Forward (+)
15670879 .. 15676100
5222 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG153100.1

Sequence Viewer

Length: 552 bp
ATGGTTGACTTTGCTCTTTCTCAAGTTGACAATCTCAACGTTCTCTGGGACTTGCTCCAAAGGCCCTTCCCTTCTTCATTCCAAACAAAAACTACCCATTTTCTCTATCACCTTGATCACACTTTCTCAGTTTGTCAGTTCTCAGAGTCGCAATACCATATTCTAGTCGCCGAGTCCATTAACCTGGCGACTCAATCTCCGGATCCAGCACTCTCGACCGGATTGGAGATATCAATGGCCGGAGTAATAACAAAGTTCGTTACTGCGTCATTGTTTATGTGGATAGTTCCTGTTGCAATATTGTATGGGTATAACAACAATTTGATTCCTGGTTCAAGCGACTTGTCTCCCCATACTCTCACTCTGTTGAGTGGATTCCTTGCTGTTGTTTCTGTCAATATAGTTATTGCATTCTATATATATATGGCAATGAAGGAACCTTCGGATAAACATGAGCCTGATCCTGCATTTCTTGCTGAGGCCAAAGCTAGTGTAAACCAGTCTACAGCTGACGCTGGCAGCTCTTCCCAATCCTCCAAGAAAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VMA21

Protein Analysis

183

Amino Acids

20.07

Weight (kDa)

4.92

Isoelectric Point (pI)

54.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VMA21 PF09446 82 - 144 3.9e-20 VMA21-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05780 AT2G31710
fragaria_vesca FvH4_3g12930 FvH4_4g01060
malus_domestica MD05G1247100.v1.1 MD10G1227700.v1.1
prunus_persica Prupe.4G117300_v2.0.a1 Prupe.4G117300_v2.0.a1
pyrus_communis pycom05g22300 pycom10g19120
rosa_chinensis RchiOBHm_Chr5g0021041
rosa_laevigata RLG00000032578
rosa_multiflora Rmu_co8487873.1_g000001
rosa_rugosa Rorug05G0063800
rosa_samantha Rh5AG153400 Rh5BG153100 Rh5CG165900 Rh5DG152700
rosa_wichuraiana Rw5G013630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 503
AccIII TCCGGA 1 cut(s) 199
AclI AACGTT 1 cut(s) 39
AclWI GGATC 3 cut(s) 197, 210, 455
AcoI YGGCCR 1 cut(s) 237
AgsI TTSAA 1 cut(s) 336
AjnI CCWGG 2 cut(s) 183, 328
AluBI AGCT 3 cut(s) 488, 509, 522
AluI AGCT 3 cut(s) 488, 509, 522
Alw26I GTCTC 1 cut(s) 351
AlwI GGATC 3 cut(s) 197, 210, 455
Aor13HI TCCGGA 1 cut(s) 199
AoxI GGCC 3 cut(s) 62, 237, 480
ApeKI GCWGC 1 cut(s) 519
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 1 cut(s) 101
BamHI GGATCC 1 cut(s) 202
BbvCI CCTCAGC 1 cut(s) 477
BbvI GCAGC 1 cut(s) 531
BciT130I CCWGG 2 cut(s) 185, 330
BclI TGATCA 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 351
BfaI CTAG 2 cut(s) 164, 489
BfmI CTRYAG 1 cut(s) 504
BisI GCNGC 1 cut(s) 520
BlsI GCNGC 1 cut(s) 521
Bme1390I CCNGG 2 cut(s) 185, 330
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 2 cut(s) 204, 438
BmrFI CCNGG 2 cut(s) 185, 330
Bpu10I CCTNAGC 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 6
BsaWI WCCGGW 2 cut(s) 199, 218
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bse1I ACTGG 1 cut(s) 499
Bse3DI GCAATG 1 cut(s) 435
BseAI TCCGGA 1 cut(s) 199
BseBI CCWGG 2 cut(s) 185, 330
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 3 cut(s) 141, 156, 468
BseNI ACTGG 1 cut(s) 499
BseXI GCAGC 1 cut(s) 531
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 3 cut(s) 64, 239, 482
BsiEI CGRYCG 1 cut(s) 219
BsiSI CCGG 3 cut(s) 200, 219, 240
BslFI GGGAC 1 cut(s) 62
BsmAI GTCTC 1 cut(s) 351
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 410
BsnI GGCC 3 cut(s) 64, 239, 482
Bsp13I TCCGGA 1 cut(s) 199
Bsp143I GATC 3 cut(s) 115, 202, 460
BspANI GGCC 3 cut(s) 64, 239, 482
BspCNI CTCAG 3 cut(s) 140, 155, 469
BspEI TCCGGA 1 cut(s) 199
BspLI GGNNCC 2 cut(s) 204, 438
BspPI GGATC 3 cut(s) 197, 210, 455
BspQI GCTCTTC 1 cut(s) 529
BsrDI GCAATG 1 cut(s) 435
BsrI ACTGG 1 cut(s) 499
BssMI GATC 3 cut(s) 115, 202, 460
Bst2UI CCWGG 2 cut(s) 185, 330
Bst6I CTCTTC 2 cut(s) 529, 540
BstAPI GCANNNNNTGC 1 cut(s) 473
BstC8I GCNNGC 1 cut(s) 517
BstDEI CTNAG 3 cut(s) 127, 142, 477
BstKTI GATC 3 cut(s) 118, 205, 463
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 3 cut(s) 115, 202, 460
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 2 cut(s) 61, 473
BstNI CCWGG 2 cut(s) 185, 330
BstSCI CCNGG 2 cut(s) 183, 328
BstSFI CTRYAG 1 cut(s) 504
BstV1I GCAGC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 202
BstXI CCANNNNNNTGG 1 cut(s) 184
BstYI RGATCY 1 cut(s) 202
BsuRI GGCC 3 cut(s) 64, 239, 482
Cac8I GCNNGC 1 cut(s) 517
Cfr13I GGNCC 1 cut(s) 63
CseI GACGC 2 cut(s) 255, 521
CviAII CATG 1 cut(s) 452
CviJI RGCY 7 cut(s) 64, 239, 457, 482, 488, 509, 522
CviKI_1 RGCY 7 cut(s) 64, 239, 457, 482, 488, 509, 522
DdeI CTNAG 3 cut(s) 127, 142, 477
DpnI GATC 3 cut(s) 117, 204, 462
DpnII GATC 3 cut(s) 115, 202, 460
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 529, 540
EarI CTCTTC 2 cut(s) 529, 540
Eco32I GATATC 1 cut(s) 231
EcoO109I RGGNCCY 1 cut(s) 63
EcoRII CCWGG 2 cut(s) 183, 328
EcoRV GATATC 1 cut(s) 231
FaeI CATG 1 cut(s) 455
FaqI GGGAC 1 cut(s) 62
FatI CATG 1 cut(s) 451
FbaI TGATCA 1 cut(s) 115
FblI GTMKAC 1 cut(s) 503
Fnu4HI GCNGC 1 cut(s) 520
Fsp4HI GCNGC 1 cut(s) 520
FspBI CTAG 2 cut(s) 164, 489
GluI GCNGC 1 cut(s) 520
HaeIII GGCC 3 cut(s) 64, 239, 482
HapII CCGG 3 cut(s) 200, 219, 240
HgaI GACGC 2 cut(s) 255, 521
Hin1II CATG 1 cut(s) 455
HincII GTYRAC 2 cut(s) 7, 28
HindII GTYRAC 2 cut(s) 7, 28
HinfI GANTC 5 cut(s) 146, 173, 190, 325, 375
HpaII CCGG 3 cut(s) 200, 219, 240
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 4 cut(s) 7, 28, 496, 504
Hpy188I TCNGA 2 cut(s) 145, 445
Hpy188III TCNNGA 2 cut(s) 200, 214
Hpy8I GTNNAC 4 cut(s) 7, 28, 496, 504
HpyAV CCTTC 4 cut(s) 76, 81, 427, 450
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 3 cut(s) 296, 410, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 473
HpyF3I CTNAG 3 cut(s) 127, 142, 477
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 1 cut(s) 455
Kpn2I TCCGGA 1 cut(s) 199
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 3 cut(s) 115, 202, 460
LguI GCTCTTC 1 cut(s) 529
LmnI GCTCC 1 cut(s) 60
Lsp1109I GCAGC 1 cut(s) 531
MaeI CTAG 2 cut(s) 164, 489
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 259
MalI GATC 3 cut(s) 117, 204, 462
MboI GATC 3 cut(s) 115, 202, 460
MboII GAAGA 2 cut(s) 66, 516
MflI RGATCY 1 cut(s) 202
MluCI AATT 1 cut(s) 319
MlyI GAGTC 3 cut(s) 155, 182, 184
MnlI CCTC 2 cut(s) 472, 544
MroI TCCGGA 1 cut(s) 199
MseI TTAA 1 cut(s) 180
MspA1I CMGCKG 1 cut(s) 509
MspI CCGG 3 cut(s) 200, 219, 240
MspR9I CCNGG 2 cut(s) 185, 330
Mva1269I GAATGC 1 cut(s) 410
MvaI CCWGG 2 cut(s) 185, 330
MwoI GCNNNNNNNGC 2 cut(s) 61, 473
NdeII GATC 3 cut(s) 115, 202, 460
NlaIII CATG 1 cut(s) 455
NlaIV GGNNCC 2 cut(s) 204, 438
NmeAIII GCCGAG 1 cut(s) 196
PciSI GCTCTTC 1 cut(s) 529
PctI GAATGC 1 cut(s) 410
PfeI GAWTC 2 cut(s) 325, 375
PkrI GCNGC 1 cut(s) 521
PleI GAGTC 3 cut(s) 154, 181, 184
PpsI GAGTC 3 cut(s) 154, 181, 184
Psp1406I AACGTT 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 183, 328
PspGI CCWGG 2 cut(s) 183, 328
PspN4I GGNNCC 2 cut(s) 204, 438
PspPI GGNCC 1 cut(s) 63
PsuI RGATCY 1 cut(s) 202
PvuII CAGCTG 1 cut(s) 509
SapI GCTCTTC 1 cut(s) 529
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 1 cut(s) 520
Sau3AI GATC 3 cut(s) 115, 202, 460
Sau96I GGNCC 1 cut(s) 63
SchI GAGTC 3 cut(s) 155, 182, 184
ScrFI CCNGG 2 cut(s) 185, 330
SetI ASST 7 cut(s) 42, 114, 186, 442, 490, 511, 524
SfcI CTRYAG 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 21
SmoI CTYRAG 1 cut(s) 21
Sse9I AATT 1 cut(s) 319
SspI AATATT 1 cut(s) 300
SspMI CTAG 2 cut(s) 164, 489
StyD4I CCNGG 2 cut(s) 183, 328
TaiI ACGT 1 cut(s) 42
TaqI TCGA 1 cut(s) 215
TasI AATT 1 cut(s) 319
TfiI GAWTC 2 cut(s) 325, 375
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TseI GCWGC 1 cut(s) 519
TspDTI ATGAA 2 cut(s) 66, 446
XmiI GTMKAC 1 cut(s) 503
XspI CTAG 2 cut(s) 164, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.