RLG00000033416

Protein CUP-SHAPED COTYLEDON 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012907
Physical Location & Seq
Forward (+)
26903786 .. 26905580
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033416

Sequence Viewer

Length: 1080 bp
ATGGACCTCATGAACCTCGGTCGCCATCTTGATCAGCTAAACTGTGTTCACCTCCACGACAACCAACTGCCGCCGGGGTTTCGCTTCCACCCTTCCGATGAGGAACTCATCAGCTACTACCTCCTCAAAAAGGTCATGGACTCCAACTTCACTTGTCGCGCCATAGCCGAAGTTGACCTCAACAAGTGCGAGCCATGGCAGCTCCCTGAGAAAGCAAAGATGGGGGAGAAAGAGTGGTACTTCTTCAGCCTCCGCGACAGGAAATACCCGACTGGCCTCCGCACCAACCGTGCCACTGAAGCCGGGTACTGGAAAGCGACCGGGAAAGACCGAGAGATTTACAGCTCCAAGAGTTGTTCCCTTCTTGGGATGAAGAAGACTTTGGTTTTTTACCGCGGCCGAGCTCCCAAGGGTGTGAAAAGTAACTGGGTCATGCATGAGTACCGCCTTGAAGGCCGATTTGCTTACAATTACCTCCCCAGGAACTCCCAGGAAGAGTGGGTCATTTCGAGGGTATTCCAGAAGGCCAAGAAGAGCAATGGACCCGAATCCAAGAAGACCCGAATGATTGGGTCCAGCGGGTACCCAGCCGAGCCCAGCTCGCCTTCTTCCTCCTCTGTCTCACTGCCACCACTTTTCGACTCCTCCCCTTACTACCCAACCATCCCTTTCTCCAACGGCAATGCTTCCCTCACCGACCGTGACAGCTGCTCCCACGACAACCGTACCCCGAGGGAGCACGTGTCCTGTTTCTCCACCAACAGCGACTCCGCCAACACCGGCACTTTCAACCATCTCATGACTTCCTTGGACCTCGCTCCGCCGCCGCAGCCGCAGCCGCAGACTTCGCTCCACTTGTCTGGTGGCTTCGGCGGCCTCTCGGACTTTCCGAGCCTCAGGTCTCTCCAGGAGAATCTCCAGCTGCCCTTCTTCTTCCCGCAGGGAATGCAGCCCTACTCCGGCGCCGGTGGCTCCTTCGACCTCGGCGACTGGAGCTCACTCGGAAACTGGCGTGGCGCCGCTGCCGAAGAGCCTAGGGCTGTGGGTCCAACCGAGGCCGATTGCATGTGGACGTACTAA

Protein Analysis

360

Amino Acids

40.42

Weight (kDa)

8.08

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 24 - 150 9.1e-49 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 582
AccB1I GGYRCC 3 cut(s) 582, 962, 1016
AccII CGCG 3 cut(s) 159, 255, 396
AcoI YGGCCR 1 cut(s) 397
AcuI CTGAAG 2 cut(s) 229, 318
AcvI CACGTG 1 cut(s) 742
AcyI GRCGYC 2 cut(s) 963, 1017
AfaI GTAC 6 cut(s) 239, 308, 443, 584, 727, 1076
AfiI CCNNNNNNNGG 4 cut(s) 130, 309, 366, 959
AflIII ACRYGT 1 cut(s) 741
AgsI TTSAA 2 cut(s) 452, 790
AjnI CCWGG 3 cut(s) 479, 489, 906
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 9 cut(s) 37, 114, 202, 345, 404, 600, 708, 922, 996
AluI AGCT 9 cut(s) 37, 114, 202, 345, 404, 600, 708, 922, 996
Alw21I GWGCWC 3 cut(s) 406, 741, 998
Alw26I GTCTC 2 cut(s) 625, 906
Ama87I CYCGRG 1 cut(s) 730
AoxI GGCC 6 cut(s) 274, 397, 454, 525, 874, 1056
ApeKI GCWGC 7 cut(s) 199, 708, 829, 835, 922, 949, 1022
Asp718I GGTACC 1 cut(s) 582
AspA2I CCTAGG 1 cut(s) 1034
AspLEI GCGC 3 cut(s) 161, 965, 1019
AspS9I GGNCC 5 cut(s) 4, 542, 573, 811, 1046
AsuC2I CCSGG 3 cut(s) 75, 304, 322
AsuHPI GGTGA 2 cut(s) 41, 685
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 5 cut(s) 4, 542, 573, 811, 1046
AvrII CCTAGG 1 cut(s) 1034
AxyI CCTNAGG 1 cut(s) 896
BaeI ACNNNNGTAYC 2 cut(s) 709, 742
BanI GGYRCC 3 cut(s) 582, 962, 1016
BanII GRGCYC 3 cut(s) 406, 597, 998
BbrPI CACGTG 1 cut(s) 742
BbsI GAAGAC 2 cut(s) 383, 563
Bbv12I GWGCWC 3 cut(s) 406, 741, 998
BbvI GCAGC 7 cut(s) 211, 695, 841, 847, 909, 961, 1009
BccI CCATC 4 cut(s) 33, 214, 671, 801
BceAI ACGGC 1 cut(s) 694
BciT130I CCWGG 3 cut(s) 481, 491, 908
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 3 cut(s) 75, 304, 322
BcoDI GTCTC 2 cut(s) 625, 906
BfaI CTAG 1 cut(s) 1035
BfoI RGCGCY 2 cut(s) 966, 1020
BglI GCCNNNNNGGC 1 cut(s) 453
BlnI CCTAGG 1 cut(s) 1034
Bme1390I CCNGG 6 cut(s) 75, 304, 322, 481, 491, 908
Bme18I GGWCC 5 cut(s) 4, 542, 573, 811, 1046
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 5 cut(s) 4, 542, 573, 811, 1046
BmiI GGNNCC 7 cut(s) 544, 574, 584, 964, 973, 1018, 1047
BmrFI CCNGG 6 cut(s) 75, 304, 322, 481, 491, 908
BmrI ACTGGG 1 cut(s) 436
BmuI ACTGGG 1 cut(s) 436
BpiI GAAGAC 2 cut(s) 383, 563
BplI GAGNNNNNCTC 2 cut(s) 584, 616
BpmI CTGGAG 3 cut(s) 890, 902, 1012
BpuMI CCSGG 3 cut(s) 75, 304, 322
BsaAI YACGTR 1 cut(s) 742
BsaHI GRCGYC 2 cut(s) 963, 1017
BsaI GGTCTC 1 cut(s) 906
BsaXI ACNNNNNCTCC 8 cut(s) 218, 248, 695, 725, 728, 752, 758, 782
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 309, 366, 959
Bse118I RCCGGY 2 cut(s) 779, 965
Bse1I ACTGG 5 cut(s) 277, 314, 431, 995, 1013
Bse21I CCTNAGG 1 cut(s) 896
Bse3DI GCAATG 2 cut(s) 544, 688
BseBI CCWGG 3 cut(s) 481, 491, 908
BseGI GGATG 2 cut(s) 375, 663
BseLI CCNNNNNNNGG 4 cut(s) 130, 309, 366, 959
BseMI GCAATG 2 cut(s) 544, 688
BseMII CTCAG 2 cut(s) 198, 910
BseNI ACTGG 5 cut(s) 277, 314, 431, 995, 1013
BseRI GAGGAG 3 cut(s) 113, 604, 634
BseX3I CGGCCG 1 cut(s) 397
BseXI GCAGC 7 cut(s) 211, 695, 841, 847, 909, 961, 1009
BseYI CCCAGC 2 cut(s) 586, 596
Bsh1236I CGCG 3 cut(s) 159, 255, 396
Bsh1285I CGRYCG 4 cut(s) 22, 321, 400, 700
BshFI GGCC 6 cut(s) 276, 399, 456, 527, 876, 1058
BshNI GGYRCC 3 cut(s) 582, 962, 1016
BsiEI CGRYCG 4 cut(s) 22, 321, 400, 700
BsiHKAI GWGCWC 3 cut(s) 406, 741, 998
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 6 cut(s) 74, 303, 321, 780, 960, 966
BslI CCNNNNNNNGG 4 cut(s) 130, 309, 366, 959
BsmAI GTCTC 2 cut(s) 625, 906
BsmI GAATGC 1 cut(s) 951
BsnI GGCC 6 cut(s) 276, 399, 456, 527, 876, 1058
Bso31I GGTCTC 1 cut(s) 906
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 4 cut(s) 406, 597, 741, 998
Bsp143I GATC 1 cut(s) 31
Bsp19I CCATGG 1 cut(s) 194
BspANI GGCC 6 cut(s) 276, 399, 456, 527, 876, 1058
BspCNI CTCAG 2 cut(s) 199, 909
BspFNI CGCG 3 cut(s) 159, 255, 396
BspHI TCATGA 2 cut(s) 9, 798
BspLI GGNNCC 7 cut(s) 544, 574, 584, 964, 973, 1018, 1047
BspQI GCTCTTC 2 cut(s) 527, 1023
BspT107I GGYRCC 3 cut(s) 582, 962, 1016
BspTNI GGTCTC 1 cut(s) 906
BsrDI GCAATG 2 cut(s) 544, 688
BsrFI RCCGGY 2 cut(s) 779, 965
BsrI ACTGG 5 cut(s) 277, 314, 431, 995, 1013
BssAI RCCGGY 2 cut(s) 779, 965
BssMI GATC 1 cut(s) 31
BssNI GRCGYC 2 cut(s) 963, 1017
BssT1I CCWWGG 4 cut(s) 194, 408, 807, 1034
Bst2UI CCWGG 3 cut(s) 481, 491, 908
Bst4CI ACNGT 4 cut(s) 44, 290, 701, 725
Bst6I CTCTTC 3 cut(s) 489, 527, 1023
BstACI GRCGYC 2 cut(s) 963, 1017
BstAPI GCANNNNNTGC 1 cut(s) 946
BstBAI YACGTR 1 cut(s) 742
BstC8I GCNNGC 2 cut(s) 191, 602
BstDEI CTNAG 2 cut(s) 207, 896
BstDSI CCRYGG 2 cut(s) 194, 394
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 2 cut(s) 375, 663
BstFNI CGCG 3 cut(s) 159, 255, 396
BstH2I RGCGCY 2 cut(s) 966, 1020
BstHHI GCGC 3 cut(s) 161, 965, 1019
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 2 cut(s) 625, 906
BstMBI GATC 1 cut(s) 31
BstMCI CGRYCG 4 cut(s) 22, 321, 400, 700
BstNI CCWGG 3 cut(s) 481, 491, 908
BstNSI RCATGY 1 cut(s) 1069
BstSCI CCNGG 6 cut(s) 73, 302, 320, 479, 489, 906
BstUI CGCG 3 cut(s) 159, 255, 396
BstV1I GCAGC 7 cut(s) 211, 695, 841, 847, 909, 961, 1009
BstV2I GAAGAC 2 cut(s) 383, 563
BstXI CCANNNNNNTGG 1 cut(s) 860
BstZI CGGCCG 1 cut(s) 397
Bsu36I CCTNAGG 1 cut(s) 896
BsuRI GGCC 6 cut(s) 276, 399, 456, 527, 876, 1058
BtgI CCRYGG 2 cut(s) 194, 394
BtsCI GGATG 2 cut(s) 375, 663
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 2 cut(s) 294, 623
Cac8I GCNNGC 2 cut(s) 191, 602
CciI TCATGA 2 cut(s) 9, 798
CfoI GCGC 3 cut(s) 161, 965, 1019
Cfr10I RCCGGY 2 cut(s) 779, 965
Cfr13I GGNCC 5 cut(s) 4, 542, 573, 811, 1046
Cfr42I CCGCGG 1 cut(s) 397
Csp6I GTAC 6 cut(s) 238, 307, 442, 583, 726, 1075
CviAII CATG 7 cut(s) 10, 136, 195, 433, 437, 799, 1066
CviQI GTAC 6 cut(s) 238, 307, 442, 583, 726, 1075
DdeI CTNAG 2 cut(s) 207, 896
DinI GGCGCC 2 cut(s) 964, 1018
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 397
EagI CGGCCG 1 cut(s) 397
Eam1104I CTCTTC 3 cut(s) 489, 527, 1023
EarI CTCTTC 3 cut(s) 489, 527, 1023
EciI GGCGGA 2 cut(s) 760, 810
Ecl136II GAGCTC 2 cut(s) 404, 996
EclXI CGGCCG 1 cut(s) 397
Eco130I CCWWGG 4 cut(s) 194, 408, 807, 1034
Eco24I GRGCYC 3 cut(s) 406, 597, 998
Eco31I GGTCTC 1 cut(s) 906
Eco47I GGWCC 5 cut(s) 4, 542, 573, 811, 1046
Eco52I CGGCCG 1 cut(s) 397
Eco53kI GAGCTC 2 cut(s) 404, 996
Eco57I CTGAAG 2 cut(s) 229, 318
Eco72I CACGTG 1 cut(s) 742
Eco81I CCTNAGG 1 cut(s) 896
Eco88I CYCGRG 1 cut(s) 730
EcoICRI GAGCTC 2 cut(s) 404, 996
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoRII CCWGG 3 cut(s) 479, 489, 906
EcoT14I CCWWGG 4 cut(s) 194, 408, 807, 1034
EcoT22I ATGCAT 1 cut(s) 438
EcoT38I GRGCYC 3 cut(s) 406, 597, 998
EgeI GGCGCC 2 cut(s) 964, 1018
EheI GGCGCC 2 cut(s) 964, 1018
ErhI CCWWGG 4 cut(s) 194, 408, 807, 1034
FaeI CATG 7 cut(s) 13, 139, 198, 436, 440, 802, 1069
FaiI YATR 8 cut(s) 11, 137, 164, 196, 434, 438, 800, 1067
FatI CATG 7 cut(s) 9, 135, 194, 432, 436, 798, 1065
FauI CCCGC 2 cut(s) 572, 945
FbaI TGATCA 1 cut(s) 31
FokI GGATG 2 cut(s) 382, 650
FriOI GRGCYC 3 cut(s) 406, 597, 998
FspBI CTAG 1 cut(s) 1035
GlaI GCGC 3 cut(s) 160, 964, 1018
GsaI CCCAGC 2 cut(s) 590, 600
GsuI CTGGAG 3 cut(s) 890, 902, 1012
HaeII RGCGCY 2 cut(s) 966, 1020
HaeIII GGCC 6 cut(s) 276, 399, 456, 527, 876, 1058
HapII CCGG 6 cut(s) 74, 303, 321, 780, 960, 966
HhaI GCGC 3 cut(s) 161, 965, 1019
Hin1I GRCGYC 2 cut(s) 963, 1017
Hin1II CATG 7 cut(s) 13, 139, 198, 436, 440, 802, 1069
Hin6I GCGC 3 cut(s) 159, 963, 1017
HinP1I GCGC 3 cut(s) 159, 963, 1017
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 5 cut(s) 140, 548, 641, 767, 913
HpaII CCGG 6 cut(s) 74, 303, 321, 780, 960, 966
HphI GGTGA 2 cut(s) 41, 685
Hpy166II GTNNAC 3 cut(s) 49, 175, 1071
Hpy188I TCNGA 4 cut(s) 97, 883, 891, 1004
Hpy188III TCNNGA 4 cut(s) 10, 29, 520, 799
Hpy8I GTNNAC 3 cut(s) 49, 175, 1071
HpyAV CCTTC 7 cut(s) 102, 371, 446, 517, 615, 937, 985
HpyCH4III ACNGT 4 cut(s) 44, 290, 701, 725
HpyCH4IV ACGT 2 cut(s) 741, 1073
HpyCH4V TGCA 3 cut(s) 436, 949, 1065
HpyF3I CTNAG 2 cut(s) 207, 896
HpySE526I ACGT 2 cut(s) 741, 1073
Hsp92I GRCGYC 2 cut(s) 963, 1017
Hsp92II CATG 7 cut(s) 13, 139, 198, 436, 440, 802, 1069
HspAI GCGC 3 cut(s) 159, 963, 1017
KasI GGCGCC 2 cut(s) 962, 1016
KpnI GGTACC 1 cut(s) 586
Ksp22I TGATCA 1 cut(s) 31
KspI CCGCGG 1 cut(s) 397
Kzo9I GATC 1 cut(s) 31
LguI GCTCTTC 2 cut(s) 527, 1023
LmnI GCTCC 9 cut(s) 207, 350, 409, 716, 736, 823, 855, 977, 993
Lsp1109I GCAGC 7 cut(s) 211, 695, 841, 847, 909, 961, 1009
MaeI CTAG 1 cut(s) 1035
MaeII ACGT 2 cut(s) 741, 1073
MaeIII GTNAC 2 cut(s) 422, 701
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MhlI GDGCHC 4 cut(s) 406, 597, 741, 998
MluCI AATT 1 cut(s) 469
Mly113I GGCGCC 2 cut(s) 963, 1017
MlyI GAGTC 3 cut(s) 134, 635, 761
MmeI TCCRAC 3 cut(s) 168, 699, 1073
Mph1103I ATGCAT 1 cut(s) 438
MspA1I CMGCKG 5 cut(s) 396, 579, 708, 922, 1022
MspI CCGG 6 cut(s) 74, 303, 321, 780, 960, 966
MspR9I CCNGG 6 cut(s) 75, 304, 322, 481, 491, 908
Mva1269I GAATGC 1 cut(s) 951
MvaI CCWGG 3 cut(s) 481, 491, 908
MvnI CGCG 3 cut(s) 159, 255, 396
NarI GGCGCC 2 cut(s) 963, 1017
NciI CCSGG 3 cut(s) 75, 304, 322
NcoI CCATGG 1 cut(s) 194
NdeII GATC 1 cut(s) 31
NlaIII CATG 7 cut(s) 13, 139, 198, 436, 440, 802, 1069
NlaIV GGNNCC 7 cut(s) 544, 574, 584, 964, 973, 1018, 1047
NmeAIII GCCGAG 3 cut(s) 425, 616, 963
NmuCI GTSAC 1 cut(s) 701
NsiI ATGCAT 1 cut(s) 438
NspI RCATGY 1 cut(s) 1069
PagI TCATGA 2 cut(s) 9, 798
PciSI GCTCTTC 2 cut(s) 527, 1023
PcsI WCGNNNNNNNCGW 2 cut(s) 887, 984
PctI GAATGC 1 cut(s) 951
PfeI GAWTC 2 cut(s) 548, 913
PfoI TCCNGGA 1 cut(s) 906
PleI GAGTC 3 cut(s) 134, 635, 761
PluTI GGCGCC 2 cut(s) 966, 1020
PmaCI CACGTG 1 cut(s) 742
PmlI CACGTG 1 cut(s) 742
PpsI GAGTC 3 cut(s) 134, 635, 761
Ppu21I YACGTR 1 cut(s) 742
Psp124BI GAGCTC 2 cut(s) 406, 998
Psp6I CCWGG 3 cut(s) 479, 489, 906
PspCI CACGTG 1 cut(s) 742
PspFI CCCAGC 2 cut(s) 586, 596
PspGI CCWGG 3 cut(s) 479, 489, 906
PspN4I GGNNCC 7 cut(s) 544, 574, 584, 964, 973, 1018, 1047
PspPI GGNCC 5 cut(s) 4, 542, 573, 811, 1046
PvuII CAGCTG 2 cut(s) 708, 922
RsaI GTAC 6 cut(s) 239, 308, 443, 584, 727, 1076
RsaNI GTAC 6 cut(s) 238, 307, 442, 583, 726, 1075
SacI GAGCTC 2 cut(s) 406, 998
SacII CCGCGG 1 cut(s) 397
SapI GCTCTTC 2 cut(s) 527, 1023
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 5 cut(s) 4, 542, 573, 811, 1046
SchI GAGTC 3 cut(s) 134, 635, 761
ScrFI CCNGG 6 cut(s) 75, 304, 322, 481, 491, 908
SduI GDGCHC 4 cut(s) 406, 597, 741, 998
SfoI GGCGCC 2 cut(s) 964, 1018
Sfr303I CCGCGG 1 cut(s) 397
SgrAI CRCCGGYG 1 cut(s) 965
SgrBI CCGCGG 1 cut(s) 397
SinI GGWCC 5 cut(s) 4, 542, 573, 811, 1046
Sse9I AATT 1 cut(s) 469
SspDI GGCGCC 2 cut(s) 962, 1016
SspMI CTAG 1 cut(s) 1035
SstI GAGCTC 2 cut(s) 406, 998
StyD4I CCNGG 6 cut(s) 73, 302, 320, 479, 489, 906
StyI CCWWGG 4 cut(s) 194, 408, 807, 1034
TaaI ACNGT 4 cut(s) 44, 290, 701, 725
TaiI ACGT 2 cut(s) 744, 1076
TaqI TCGA 3 cut(s) 509, 639, 978
TaqII GACCGA 2 cut(s) 8, 345
TasI AATT 1 cut(s) 469
TauI GCSGC 8 cut(s) 73, 399, 826, 829, 835, 841, 876, 1022
TfiI GAWTC 2 cut(s) 548, 913
TscAI CASTG 2 cut(s) 301, 630
TseFI GTSAC 1 cut(s) 701
TseI GCWGC 7 cut(s) 199, 708, 829, 835, 922, 949, 1022
Tsp45I GTSAC 1 cut(s) 701
TspDTI ATGAA 2 cut(s) 26, 386
TspRI CASTG 2 cut(s) 301, 630
VpaK11BI GGWCC 5 cut(s) 4, 542, 573, 811, 1046
XagI CCTNNNNNAGG 1 cut(s) 128
XceI RCATGY 1 cut(s) 1069
XcmI CCANNNNNNNNNTGG 1 cut(s) 860
XmaJI CCTAGG 1 cut(s) 1034
XspI CTAG 1 cut(s) 1035
Zsp2I ATGCAT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.