Rh5CG254200

Protein CUP-SHAPED COTYLEDON 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0012907
Physical Location & Seq
Forward (+)
26937090 .. 26939963
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG254200.1

Sequence Viewer

Length: 1083 bp
ATGGACATCATGAACCTCGGTCGCCATCTTGATCAGCTAAACGGTGTTCACCTCCACGACAACCAACTGCCGCCGGGGTTTCGCTTCCACCCTTCCGACGAGGAACTCATCAGCTACTACCTCCTCAAAAAGGTCATGGACTCCAACTTCACTTGTCGCGCCATAGCCGAAGTTGACCTCAACAAGTGCGAGCCATGGCAGCTCCCTGAGAAAGCAAAGATGGGGGAGAAAGAGTGGTACTTCTTCAGCCTCCGCGACAGGAAATACCCGACTGGCCTTCGCACCAACCGTGCCACTGAAGCCGGGTACTGGAAAGCCACCGGGAAAGACCGAGAGATTTACAGCTCCAAGACTTGTTCCCTTCTTGGGATGAAGAAGACTTTGGTTTTTTACCGCGGCCGAGCTCCCAAGGGTGTGAAAAGTAACTGGGTCATGCATGAGTACCGCCTTGAAGGCCGATTTGCTTACAATTACCTCCCCAGGAACTCCCAGGAAGAGTGGGTCATTTCGAGGGTATTCCAGAAGGCCAAGAAGAGCAATGGACCCGAATCCAAGAAGACCCGAATGATTGGGTCCAGCGGGTACCCAGCCGAGCCCAGCTCGCCTTCTTCCTCCTCTGTCTCACTGCCACCACTTTTCGACTCCTCCCCTTACTACCCAACCGTCCCTTTCTCCAACGGCAATGGTTCCCTCACCGACCGTGACAGCTGCTCCCACGACAACCGTACCCCGAGGGAGCACGTGTCCTGTTTCTCCACCAACAGCGACTCCGCCAACACCGGCACTTTCAACCATCTCATGACTTCCTTGGACCTCGCTCCGCCGCCGCAGCCGCAGACTTCGCTCCACTTGTCTGGCGGCTTCGGCGGCCTCTCGGACTTTCCGAGCCTCAGGTCTCTCCAGGATAATCTCCACCTGCCCTTCTTCTTCCCGCAGGGACTGCAGCCCCACCAGCCCTACTCCGGGGCCGGTGGCTCCTTCGACCTCGGTGACTGGAGTTCACTCGGAAACTGGCGTGGCGCCGCTGCCGAAGAGCCCAGGGCTGTGGGTCCAACCGAGGCCGATTGCATGTGGACGTACTAA

Protein Analysis

360

Amino Acids

40.47

Weight (kDa)

8.12

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 24 - 150 9.1e-49 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 924
Acc36I ACCTGC 1 cut(s) 924
Acc65I GGTACC 1 cut(s) 582
AccB1I GGYRCC 2 cut(s) 582, 1019
AccII CGCG 3 cut(s) 159, 255, 396
AcoI YGGCCR 1 cut(s) 397
AcuI CTGAAG 2 cut(s) 229, 318
AcvI CACGTG 1 cut(s) 742
AcyI GRCGYC 1 cut(s) 1020
AfaI GTAC 6 cut(s) 239, 308, 443, 584, 727, 1079
AfiI CCNNNNNNNGG 5 cut(s) 130, 309, 366, 962, 963
AflIII ACRYGT 1 cut(s) 741
AgsI TTSAA 2 cut(s) 452, 790
AjnI CCWGG 4 cut(s) 479, 489, 900, 1037
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 7 cut(s) 37, 114, 202, 345, 404, 600, 708
AluI AGCT 7 cut(s) 37, 114, 202, 345, 404, 600, 708
Alw21I GWGCWC 2 cut(s) 406, 741
Alw26I GTCTC 2 cut(s) 625, 900
AlwNI CAGNNNCTG 1 cut(s) 940
Ama87I CYCGRG 1 cut(s) 730
AoxI GGCC 7 cut(s) 274, 397, 454, 525, 868, 966, 1059
ApeKI GCWGC 5 cut(s) 199, 708, 829, 943, 1025
Asp718I GGTACC 1 cut(s) 582
AspLEI GCGC 2 cut(s) 161, 1022
AspS9I GGNCC 5 cut(s) 542, 573, 811, 966, 1049
AsuC2I CCSGG 4 cut(s) 75, 304, 322, 964
AsuHPI GGTGA 3 cut(s) 41, 685, 1001
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 4 cut(s) 542, 573, 811, 1049
AxyI CCTNAGG 1 cut(s) 890
BaeI ACNNNNGTAYC 2 cut(s) 709, 742
BanI GGYRCC 2 cut(s) 582, 1019
BanII GRGCYC 3 cut(s) 406, 597, 1038
BbrPI CACGTG 1 cut(s) 742
BbsI GAAGAC 2 cut(s) 383, 563
Bbv12I GWGCWC 2 cut(s) 406, 741
BbvI GCAGC 5 cut(s) 211, 695, 841, 955, 1012
BccI CCATC 3 cut(s) 33, 214, 801
BceAI ACGGC 1 cut(s) 694
BciT130I CCWGG 4 cut(s) 481, 491, 902, 1039
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 4 cut(s) 75, 304, 322, 964
BcoDI GTCTC 2 cut(s) 625, 900
BfmI CTRYAG 1 cut(s) 941
BfoI RGCGCY 1 cut(s) 1023
BfuAI ACCTGC 1 cut(s) 924
BglI GCCNNNNNGGC 1 cut(s) 453
Bme1390I CCNGG 8 cut(s) 75, 304, 322, 481, 491, 902, 964, 1039
Bme18I GGWCC 4 cut(s) 542, 573, 811, 1049
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 5 cut(s) 542, 573, 811, 966, 1049
BmiI GGNNCC 8 cut(s) 544, 574, 584, 688, 967, 976, 1021, 1050
BmrFI CCNGG 8 cut(s) 75, 304, 322, 481, 491, 902, 964, 1039
BmrI ACTGGG 1 cut(s) 436
BmuI ACTGGG 1 cut(s) 436
BpiI GAAGAC 2 cut(s) 383, 563
BplI GAGNNNNNCTC 2 cut(s) 584, 616
BpmI CTGGAG 2 cut(s) 884, 1015
BpuMI CCSGG 4 cut(s) 75, 304, 322, 964
BsaAI YACGTR 1 cut(s) 742
BsaHI GRCGYC 1 cut(s) 1020
BsaI GGTCTC 1 cut(s) 900
BsaXI ACNNNNNCTCC 8 cut(s) 218, 248, 695, 725, 728, 752, 758, 782
Bsc4I CCNNNNNNNGG 5 cut(s) 130, 309, 366, 962, 963
Bse118I RCCGGY 2 cut(s) 779, 968
Bse1I ACTGG 5 cut(s) 277, 314, 431, 998, 1016
Bse21I CCTNAGG 1 cut(s) 890
Bse3DI GCAATG 2 cut(s) 544, 688
BseBI CCWGG 4 cut(s) 481, 491, 902, 1039
BseGI GGATG 1 cut(s) 375
BseLI CCNNNNNNNGG 5 cut(s) 130, 309, 366, 962, 963
BseMI GCAATG 2 cut(s) 544, 688
BseMII CTCAG 2 cut(s) 198, 904
BseNI ACTGG 5 cut(s) 277, 314, 431, 998, 1016
BseRI GAGGAG 3 cut(s) 113, 604, 634
BseX3I CGGCCG 1 cut(s) 397
BseXI GCAGC 5 cut(s) 211, 695, 841, 955, 1012
BseYI CCCAGC 2 cut(s) 586, 596
Bsh1236I CGCG 3 cut(s) 159, 255, 396
Bsh1285I CGRYCG 3 cut(s) 22, 400, 700
BshFI GGCC 7 cut(s) 276, 399, 456, 527, 870, 968, 1061
BshNI GGYRCC 2 cut(s) 582, 1019
BsiEI CGRYCG 3 cut(s) 22, 400, 700
BsiHKAI GWGCWC 2 cut(s) 406, 741
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 6 cut(s) 74, 303, 321, 780, 963, 969
BslFI GGGAC 2 cut(s) 650, 951
BslI CCNNNNNNNGG 5 cut(s) 130, 309, 366, 962, 963
BsmAI GTCTC 2 cut(s) 625, 900
BsmFI GGGAC 2 cut(s) 650, 951
BsnI GGCC 7 cut(s) 276, 399, 456, 527, 870, 968, 1061
Bso31I GGTCTC 1 cut(s) 900
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 4 cut(s) 406, 597, 741, 1038
Bsp143I GATC 1 cut(s) 31
Bsp19I CCATGG 1 cut(s) 194
BspANI GGCC 7 cut(s) 276, 399, 456, 527, 870, 968, 1061
BspCNI CTCAG 2 cut(s) 199, 903
BspFNI CGCG 3 cut(s) 159, 255, 396
BspHI TCATGA 2 cut(s) 9, 798
BspLI GGNNCC 8 cut(s) 544, 574, 584, 688, 967, 976, 1021, 1050
BspMAI CTGCAG 1 cut(s) 945
BspMI ACCTGC 1 cut(s) 924
BspQI GCTCTTC 2 cut(s) 527, 1026
BspT107I GGYRCC 2 cut(s) 582, 1019
BspTNI GGTCTC 1 cut(s) 900
BsrDI GCAATG 2 cut(s) 544, 688
BsrFI RCCGGY 2 cut(s) 779, 968
BsrI ACTGG 5 cut(s) 277, 314, 431, 998, 1016
BssAI RCCGGY 2 cut(s) 779, 968
BssMI GATC 1 cut(s) 31
BssNI GRCGYC 1 cut(s) 1020
BssT1I CCWWGG 3 cut(s) 194, 408, 807
Bst2UI CCWGG 4 cut(s) 481, 491, 902, 1039
Bst4CI ACNGT 5 cut(s) 44, 290, 664, 701, 725
Bst6I CTCTTC 3 cut(s) 489, 527, 1026
BstACI GRCGYC 1 cut(s) 1020
BstAPI GCANNNNNTGC 1 cut(s) 940
BstBAI YACGTR 1 cut(s) 742
BstC8I GCNNGC 2 cut(s) 191, 602
BstDEI CTNAG 2 cut(s) 207, 890
BstDSI CCRYGG 2 cut(s) 194, 394
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 1 cut(s) 375
BstFNI CGCG 3 cut(s) 159, 255, 396
BstH2I RGCGCY 1 cut(s) 1023
BstHHI GCGC 2 cut(s) 161, 1022
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 2 cut(s) 625, 900
BstMBI GATC 1 cut(s) 31
BstMCI CGRYCG 3 cut(s) 22, 400, 700
BstNI CCWGG 4 cut(s) 481, 491, 902, 1039
BstNSI RCATGY 1 cut(s) 1072
BstSCI CCNGG 8 cut(s) 73, 302, 320, 479, 489, 900, 962, 1037
BstSFI CTRYAG 1 cut(s) 941
BstUI CGCG 3 cut(s) 159, 255, 396
BstV1I GCAGC 5 cut(s) 211, 695, 841, 955, 1012
BstV2I GAAGAC 2 cut(s) 383, 563
BstXI CCANNNNNNTGG 2 cut(s) 854, 1045
BstZI CGGCCG 1 cut(s) 397
Bsu36I CCTNAGG 1 cut(s) 890
BsuRI GGCC 7 cut(s) 276, 399, 456, 527, 870, 968, 1061
BtgI CCRYGG 2 cut(s) 194, 394
BtsCI GGATG 1 cut(s) 375
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 2 cut(s) 294, 623
BveI ACCTGC 1 cut(s) 924
Cac8I GCNNGC 2 cut(s) 191, 602
CaiI CAGNNNCTG 1 cut(s) 940
CciI TCATGA 2 cut(s) 9, 798
CfoI GCGC 2 cut(s) 161, 1022
Cfr10I RCCGGY 2 cut(s) 779, 968
Cfr13I GGNCC 5 cut(s) 542, 573, 811, 966, 1049
Cfr42I CCGCGG 1 cut(s) 397
Csp6I GTAC 6 cut(s) 238, 307, 442, 583, 726, 1078
CviAII CATG 7 cut(s) 10, 136, 195, 433, 437, 799, 1069
CviQI GTAC 6 cut(s) 238, 307, 442, 583, 726, 1078
DdeI CTNAG 2 cut(s) 207, 890
DinI GGCGCC 1 cut(s) 1021
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 397
EagI CGGCCG 1 cut(s) 397
Eam1104I CTCTTC 3 cut(s) 489, 527, 1026
EarI CTCTTC 3 cut(s) 489, 527, 1026
EciI GGCGGA 2 cut(s) 760, 810
Ecl136II GAGCTC 1 cut(s) 404
EclXI CGGCCG 1 cut(s) 397
Eco130I CCWWGG 3 cut(s) 194, 408, 807
Eco24I GRGCYC 3 cut(s) 406, 597, 1038
Eco31I GGTCTC 1 cut(s) 900
Eco47I GGWCC 4 cut(s) 542, 573, 811, 1049
Eco52I CGGCCG 1 cut(s) 397
Eco53kI GAGCTC 1 cut(s) 404
Eco57I CTGAAG 2 cut(s) 229, 318
Eco72I CACGTG 1 cut(s) 742
Eco81I CCTNAGG 1 cut(s) 890
Eco88I CYCGRG 1 cut(s) 730
EcoICRI GAGCTC 1 cut(s) 404
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoRII CCWGG 4 cut(s) 479, 489, 900, 1037
EcoT14I CCWWGG 3 cut(s) 194, 408, 807
EcoT22I ATGCAT 1 cut(s) 438
EcoT38I GRGCYC 3 cut(s) 406, 597, 1038
EgeI GGCGCC 1 cut(s) 1021
EheI GGCGCC 1 cut(s) 1021
ErhI CCWWGG 3 cut(s) 194, 408, 807
FaeI CATG 7 cut(s) 13, 139, 198, 436, 440, 802, 1072
FaiI YATR 8 cut(s) 11, 137, 164, 196, 434, 438, 800, 1070
FaqI GGGAC 2 cut(s) 650, 951
FatI CATG 7 cut(s) 9, 135, 194, 432, 436, 798, 1068
FauI CCCGC 2 cut(s) 572, 939
FbaI TGATCA 1 cut(s) 31
FokI GGATG 1 cut(s) 382
FriOI GRGCYC 3 cut(s) 406, 597, 1038
GlaI GCGC 2 cut(s) 160, 1021
GsaI CCCAGC 2 cut(s) 590, 600
GsuI CTGGAG 2 cut(s) 884, 1015
HaeII RGCGCY 1 cut(s) 1023
HaeIII GGCC 7 cut(s) 276, 399, 456, 527, 870, 968, 1061
HapII CCGG 6 cut(s) 74, 303, 321, 780, 963, 969
HhaI GCGC 2 cut(s) 161, 1022
Hin1I GRCGYC 1 cut(s) 1020
Hin1II CATG 7 cut(s) 13, 139, 198, 436, 440, 802, 1072
Hin6I GCGC 2 cut(s) 159, 1020
HinP1I GCGC 2 cut(s) 159, 1020
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 4 cut(s) 140, 548, 641, 767
HpaII CCGG 6 cut(s) 74, 303, 321, 780, 963, 969
HphI GGTGA 3 cut(s) 41, 685, 1001
Hpy166II GTNNAC 4 cut(s) 49, 175, 1001, 1074
Hpy188I TCNGA 4 cut(s) 97, 877, 885, 1007
Hpy188III TCNNGA 4 cut(s) 10, 29, 520, 799
Hpy8I GTNNAC 4 cut(s) 49, 175, 1001, 1074
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 8 cut(s) 102, 287, 371, 446, 517, 615, 931, 988
HpyCH4III ACNGT 5 cut(s) 44, 290, 664, 701, 725
HpyCH4IV ACGT 2 cut(s) 741, 1076
HpyCH4V TGCA 3 cut(s) 436, 943, 1068
HpyF3I CTNAG 2 cut(s) 207, 890
HpySE526I ACGT 2 cut(s) 741, 1076
Hsp92I GRCGYC 1 cut(s) 1020
Hsp92II CATG 7 cut(s) 13, 139, 198, 436, 440, 802, 1072
HspAI GCGC 2 cut(s) 159, 1020
KasI GGCGCC 1 cut(s) 1019
KpnI GGTACC 1 cut(s) 586
Ksp22I TGATCA 1 cut(s) 31
KspI CCGCGG 1 cut(s) 397
Kzo9I GATC 1 cut(s) 31
LguI GCTCTTC 2 cut(s) 527, 1026
LmnI GCTCC 8 cut(s) 207, 350, 409, 716, 736, 823, 849, 980
Lsp1109I GCAGC 5 cut(s) 211, 695, 841, 955, 1012
MaeII ACGT 2 cut(s) 741, 1076
MaeIII GTNAC 3 cut(s) 422, 701, 989
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MhlI GDGCHC 4 cut(s) 406, 597, 741, 1038
MluCI AATT 1 cut(s) 469
Mly113I GGCGCC 1 cut(s) 1020
MlyI GAGTC 3 cut(s) 134, 635, 761
MmeI TCCRAC 4 cut(s) 120, 168, 699, 1076
Mph1103I ATGCAT 1 cut(s) 438
MspA1I CMGCKG 4 cut(s) 396, 579, 708, 1025
MspI CCGG 6 cut(s) 74, 303, 321, 780, 963, 969
MspR9I CCNGG 8 cut(s) 75, 304, 322, 481, 491, 902, 964, 1039
MvaI CCWGG 4 cut(s) 481, 491, 902, 1039
MvnI CGCG 3 cut(s) 159, 255, 396
NarI GGCGCC 1 cut(s) 1020
NciI CCSGG 4 cut(s) 75, 304, 322, 964
NcoI CCATGG 1 cut(s) 194
NdeII GATC 1 cut(s) 31
NlaIII CATG 7 cut(s) 13, 139, 198, 436, 440, 802, 1072
NlaIV GGNNCC 8 cut(s) 544, 574, 584, 688, 967, 976, 1021, 1050
NmeAIII GCCGAG 2 cut(s) 425, 616
NmuCI GTSAC 2 cut(s) 701, 989
NsiI ATGCAT 1 cut(s) 438
NspI RCATGY 1 cut(s) 1072
PagI TCATGA 2 cut(s) 9, 798
PaqCI CACCTGC 1 cut(s) 924
PasI CCCWGGG 1 cut(s) 1038
PciSI GCTCTTC 2 cut(s) 527, 1026
PcsI WCGNNNNNNNCGW 2 cut(s) 286, 881
PfeI GAWTC 1 cut(s) 548
PfoI TCCNGGA 1 cut(s) 900
PleI GAGTC 3 cut(s) 134, 635, 761
PluTI GGCGCC 1 cut(s) 1023
PmaCI CACGTG 1 cut(s) 742
PmlI CACGTG 1 cut(s) 742
PpsI GAGTC 3 cut(s) 134, 635, 761
Ppu21I YACGTR 1 cut(s) 742
Psp124BI GAGCTC 1 cut(s) 406
Psp6I CCWGG 4 cut(s) 479, 489, 900, 1037
PspCI CACGTG 1 cut(s) 742
PspFI CCCAGC 2 cut(s) 586, 596
PspGI CCWGG 4 cut(s) 479, 489, 900, 1037
PspN4I GGNNCC 8 cut(s) 544, 574, 584, 688, 967, 976, 1021, 1050
PspPI GGNCC 5 cut(s) 542, 573, 811, 966, 1049
PstI CTGCAG 1 cut(s) 945
PstNI CAGNNNCTG 1 cut(s) 940
PvuII CAGCTG 1 cut(s) 708
RsaI GTAC 6 cut(s) 239, 308, 443, 584, 727, 1079
RsaNI GTAC 6 cut(s) 238, 307, 442, 583, 726, 1078
SacI GAGCTC 1 cut(s) 406
SacII CCGCGG 1 cut(s) 397
SapI GCTCTTC 2 cut(s) 527, 1026
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 5 cut(s) 542, 573, 811, 966, 1049
SchI GAGTC 3 cut(s) 134, 635, 761
ScrFI CCNGG 8 cut(s) 75, 304, 322, 481, 491, 902, 964, 1039
SduI GDGCHC 4 cut(s) 406, 597, 741, 1038
SfcI CTRYAG 1 cut(s) 941
SfoI GGCGCC 1 cut(s) 1021
Sfr303I CCGCGG 1 cut(s) 397
SgrBI CCGCGG 1 cut(s) 397
SinI GGWCC 4 cut(s) 542, 573, 811, 1049
Sse9I AATT 1 cut(s) 469
SspDI GGCGCC 1 cut(s) 1019
SstI GAGCTC 1 cut(s) 406
StyD4I CCNGG 8 cut(s) 73, 302, 320, 479, 489, 900, 962, 1037
StyI CCWWGG 3 cut(s) 194, 408, 807
TaaI ACNGT 5 cut(s) 44, 290, 664, 701, 725
TaiI ACGT 2 cut(s) 744, 1079
TaqI TCGA 3 cut(s) 509, 639, 981
TaqII GACCGA 2 cut(s) 8, 345
TasI AATT 1 cut(s) 469
TauI GCSGC 8 cut(s) 73, 399, 826, 829, 835, 861, 870, 1025
TfiI GAWTC 1 cut(s) 548
TscAI CASTG 2 cut(s) 301, 630
TseFI GTSAC 2 cut(s) 701, 989
TseI GCWGC 5 cut(s) 199, 708, 829, 943, 1025
Tsp45I GTSAC 2 cut(s) 701, 989
TspDTI ATGAA 2 cut(s) 26, 386
TspRI CASTG 2 cut(s) 301, 630
VpaK11BI GGWCC 4 cut(s) 542, 573, 811, 1049
XagI CCTNNNNNAGG 1 cut(s) 128
XceI RCATGY 1 cut(s) 1072
Zsp2I ATGCAT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.