RLG00000034352

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016820
Physical Location & Seq
Forward (+)
41211665 .. 41213114
1450 bp
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UTR
Exon/CDS
Intron
RLM00000034352

Sequence Viewer

Length: 414 bp
ATGTGTTTGGCGTACGAGAATCTGAGGGCTTCTCAGGAAGAGTTCTTCAAGACCCGACTTGAACCTGAGGGGTTGAAGGAGTCCTACAGGGCCGATCGGGAATTCAAGGATGAAATCGAGAAGGGGTTGCTGGTTCAAGACAGGGACTTCGACTTGGGATGGAAGTCTGGGAAGATGTGGTCTTATTTGATTGCAAAGAGTAAGGAAGGCGGGGCGGATGTGATCCAGACTTATGCATTTTGGAGCGGTCACAAGCGCCAGAGAGGACAGGTGTGCCATGAGTTTTTGTCCACAGCTAGTCCGAGGAAGAGATGCAGCAGTTTGTTAAAAAGATGGTGGATCTTATGTGAGAGGAAGAGCTATTTTCTTGGCAAGGTGGTCCAGCAATTATGCTACAGCTACAAAAGATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

138

Amino Acids

16.29

Weight (kDa)

9.32

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 58 - 91 8.7e-06 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 412
AccBSI CCGCTC 1 cut(s) 246
AciI CCGC 3 cut(s) 210, 215, 246
AclWI GGATC 2 cut(s) 217, 347
AcsI RAATTY 1 cut(s) 101
AfaI GTAC 1 cut(s) 14
AgsI TTSAA 5 cut(s) 49, 62, 76, 106, 137
AluBI AGCT 3 cut(s) 296, 360, 399
AluI AGCT 3 cut(s) 296, 360, 399
AlwI GGATC 2 cut(s) 217, 347
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 315
ApoI RAATTY 1 cut(s) 101
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 2 cut(s) 90, 379
AvaII GGWCC 1 cut(s) 379
AxyI CCTNAGG 1 cut(s) 66
BarI GAAGNNNNNNTAC 2 cut(s) 68, 100
BbvI GCAGC 1 cut(s) 327
BccI CCATC 2 cut(s) 153, 327
BfaI CTAG 1 cut(s) 297
BfmI CTRYAG 2 cut(s) 85, 394
BfoI RGCGCY 1 cut(s) 259
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 90, 379
BmsI GCATC 1 cut(s) 302
BplI GAGNNNNNCTC 2 cut(s) 16, 48
BsaJI CCNNGG 1 cut(s) 302
Bse21I CCTNAGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 302
BseGI GGATG 3 cut(s) 115, 164, 223
BseMII CTCAG 3 cut(s) 14, 47, 57
BseXI GCAGC 1 cut(s) 327
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 1 cut(s) 92
BsiEI CGRYCG 1 cut(s) 97
BsiWI CGTACG 1 cut(s) 12
BslFI GGGAC 1 cut(s) 158
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 1 cut(s) 92
Bsp143I GATC 3 cut(s) 94, 222, 339
BspACI CCGC 3 cut(s) 210, 215, 246
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 3 cut(s) 15, 46, 58
BspPI GGATC 2 cut(s) 217, 347
BspQI GCTCTTC 1 cut(s) 350
BsrBI CCGCTC 1 cut(s) 246
BssECI CCNNGG 1 cut(s) 302
BssMI GATC 3 cut(s) 94, 222, 339
Bst6I CTCTTC 3 cut(s) 33, 302, 350
BstDEI CTNAG 3 cut(s) 23, 33, 66
BstF5I GGATG 3 cut(s) 115, 164, 223
BstH2I RGCGCY 1 cut(s) 259
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 3 cut(s) 97, 225, 342
BstMBI GATC 3 cut(s) 94, 222, 339
BstMCI CGRYCG 1 cut(s) 97
BstSFI CTRYAG 2 cut(s) 85, 394
BstV1I GCAGC 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 339
BstYI RGATCY 1 cut(s) 339
Bsu36I CCTNAGG 1 cut(s) 66
BsuRI GGCC 1 cut(s) 92
BtsCI GGATG 3 cut(s) 115, 164, 223
CfoI GCGC 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 90, 379
Csp6I GTAC 1 cut(s) 13
CviAII CATG 1 cut(s) 278
CviJI RGCY 5 cut(s) 29, 92, 296, 360, 399
CviKI_1 RGCY 5 cut(s) 29, 92, 296, 360, 399
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 3 cut(s) 23, 33, 66
DpnI GATC 3 cut(s) 96, 224, 341
DpnII GATC 3 cut(s) 94, 222, 339
Eam1104I CTCTTC 3 cut(s) 33, 302, 350
EarI CTCTTC 3 cut(s) 33, 302, 350
EciI GGCGGA 1 cut(s) 230
Eco47I GGWCC 1 cut(s) 379
Eco81I CCTNAGG 1 cut(s) 66
EcoRI GAATTC 1 cut(s) 101
EcoT22I ATGCAT 1 cut(s) 238
FaeI CATG 1 cut(s) 281
FaiI YATR 5 cut(s) 234, 279, 346, 391, 412
FaqI GGGAC 1 cut(s) 158
FatI CATG 1 cut(s) 277
FauI CCCGC 1 cut(s) 203
Fnu4HI GCNGC 1 cut(s) 316
FokI GGATG 3 cut(s) 122, 171, 230
Fsp4HI GCNGC 1 cut(s) 316
FspBI CTAG 1 cut(s) 297
GlaI GCGC 1 cut(s) 257
GluI GCNGC 1 cut(s) 316
HaeII RGCGCY 1 cut(s) 259
HaeIII GGCC 1 cut(s) 92
HhaI GCGC 1 cut(s) 258
Hin1II CATG 1 cut(s) 281
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HinfI GANTC 2 cut(s) 19, 80
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 2 cut(s) 24, 303
Hpy188III TCNNGA 6 cut(s) 35, 49, 98, 118, 137, 226
Hpy8I GTNNAC 1 cut(s) 291
HpyAV CCTTC 3 cut(s) 70, 115, 200
HpyCH4V TGCA 3 cut(s) 194, 236, 315
HpyF3I CTNAG 3 cut(s) 23, 33, 66
Hsp92II CATG 1 cut(s) 281
HspAI GCGC 1 cut(s) 256
Kzo9I GATC 3 cut(s) 94, 222, 339
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 1 cut(s) 243
Lsp1109I GCAGC 1 cut(s) 327
LweI GCATC 1 cut(s) 302
MaeI CTAG 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 248
MalI GATC 3 cut(s) 96, 224, 341
MbiI CCGCTC 1 cut(s) 246
MboI GATC 3 cut(s) 94, 222, 339
MboII GAAGA 5 cut(s) 37, 50, 184, 319, 367
MflI RGATCY 1 cut(s) 339
MluCI AATT 2 cut(s) 101, 386
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 5 cut(s) 18, 61, 257, 297, 345
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 1 cut(s) 326
NdeII GATC 3 cut(s) 94, 222, 339
NlaIII CATG 1 cut(s) 281
NmuCI GTSAC 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 238
PciSI GCTCTTC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 19
Pfl23II CGTACG 1 cut(s) 12
PkrI GCNGC 1 cut(s) 317
Ple19I CGATCG 1 cut(s) 97
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PsiI TTATAA 1 cut(s) 412
PspLI CGTACG 1 cut(s) 12
PspPI GGNCC 2 cut(s) 90, 379
PsuI RGATCY 1 cut(s) 339
PvuI CGATCG 1 cut(s) 97
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 1 cut(s) 316
Sau3AI GATC 3 cut(s) 94, 222, 339
Sau96I GGNCC 2 cut(s) 90, 379
SchI GAGTC 1 cut(s) 89
SetI ASST 6 cut(s) 67, 273, 298, 362, 378, 401
SfaNI GCATC 1 cut(s) 302
SfcI CTRYAG 2 cut(s) 85, 394
SinI GGWCC 1 cut(s) 379
Sse9I AATT 2 cut(s) 101, 386
SsiI CCGC 3 cut(s) 210, 215, 246
SspMI CTAG 1 cut(s) 297
TaqI TCGA 2 cut(s) 117, 150
TasI AATT 2 cut(s) 101, 386
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 1 cut(s) 315
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 1 cut(s) 126
VpaK11BI GGWCC 1 cut(s) 379
XapI RAATTY 1 cut(s) 101
XspI CTAG 1 cut(s) 297
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.