Rroxscaffold_3G00260570

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016820
Physical Location & Seq
Reverse (-)
54970028 .. 54974832
4805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00260570.1

Sequence Viewer

Length: 345 bp
ATGGGGTTGGATTCAAACCCAATATATCCGACATGGACATATATAATGGAAAGAGTTCTTGAGGGCTTCTCAGAAGAGTTCTTCAAGACTGGACTTGAACGTAAGGGGTTGAAGGAGTCTACAGTGGCCGATCGGGAATTCAAGGATGAAATCGAGAAGGGGTTGTCGATTCAAGACAGGACTTCGACTTGGGATGGAAGTCCGATGTGGTCTTATTTGATTGCAAAGAGTAAGGAAGGCGGGGCAGATGTGATCCGTACTTATGCATTTTGGAGCGGTCACAAGCCCCGCAGAGGACAGGAAGAGATGCAGCAGTTTGTTAAAAAGATGGTGGATCTTATGTGA

Protein Analysis

114

Amino Acids

13.28

Weight (kDa)

5.49

Isoelectric Point (pI)

30.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 69 - 103 1e-05 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 276
AccI GTMKAC 1 cut(s) 119
AciI CCGC 3 cut(s) 240, 276, 289
AclWI GGATC 2 cut(s) 247, 342
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 1 cut(s) 137
AfaI GTAC 1 cut(s) 259
AfiI CCNNNNNNNGG 1 cut(s) 293
AgsI TTSAA 6 cut(s) 15, 85, 98, 112, 142, 173
AlwI GGATC 2 cut(s) 247, 342
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 310
ApoI RAATTY 1 cut(s) 137
Asp700I GAANNNNTTC 1 cut(s) 54
BbvI GCAGC 1 cut(s) 322
BccI CCATC 2 cut(s) 188, 322
BfmI CTRYAG 1 cut(s) 120
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
BmsI GCATC 1 cut(s) 297
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpuEI CTTGAG 1 cut(s) 80
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 94
BseGI GGATG 2 cut(s) 151, 199
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 1 cut(s) 94
BseXI GCAGC 1 cut(s) 322
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 293
BsnI GGCC 1 cut(s) 128
Bsp143I GATC 3 cut(s) 130, 252, 334
BspACI CCGC 3 cut(s) 240, 276, 289
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 1 cut(s) 83
BspPI GGATC 2 cut(s) 247, 342
BsrBI CCGCTC 1 cut(s) 276
BsrI ACTGG 1 cut(s) 94
BssMI GATC 3 cut(s) 130, 252, 334
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 2 cut(s) 69, 297
BstDEI CTNAG 1 cut(s) 70
BstF5I GGATG 2 cut(s) 151, 199
BstKTI GATC 3 cut(s) 133, 255, 337
BstMBI GATC 3 cut(s) 130, 252, 334
BstMCI CGRYCG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 322
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 1 cut(s) 128
BtsCI GGATG 2 cut(s) 151, 199
BtsIMutI CAGTG 1 cut(s) 129
Csp6I GTAC 1 cut(s) 258
CviAII CATG 1 cut(s) 33
CviJI RGCY 3 cut(s) 66, 128, 286
CviKI_1 RGCY 3 cut(s) 66, 128, 286
CviQI GTAC 1 cut(s) 258
DdeI CTNAG 1 cut(s) 70
DpnI GATC 3 cut(s) 132, 254, 336
DpnII GATC 3 cut(s) 130, 252, 334
EaeI YGGCCR 1 cut(s) 126
Eam1104I CTCTTC 2 cut(s) 69, 297
EarI CTCTTC 2 cut(s) 69, 297
EcoRI GAATTC 1 cut(s) 137
EcoT22I ATGCAT 1 cut(s) 268
FaeI CATG 1 cut(s) 36
FaiI YATR 7 cut(s) 25, 34, 40, 42, 44, 264, 341
FatI CATG 1 cut(s) 32
FauI CCCGC 2 cut(s) 233, 296
FblI GTMKAC 1 cut(s) 119
Fnu4HI GCNGC 1 cut(s) 311
FokI GGATG 2 cut(s) 158, 206
Fsp4HI GCNGC 1 cut(s) 311
GluI GCNGC 1 cut(s) 311
HaeIII GGCC 1 cut(s) 128
Hin1II CATG 1 cut(s) 36
HinfI GANTC 3 cut(s) 11, 116, 169
Hpy166II GTNNAC 1 cut(s) 120
Hpy188I TCNGA 3 cut(s) 30, 73, 204
Hpy188III TCNNGA 5 cut(s) 59, 85, 134, 154, 173
Hpy8I GTNNAC 1 cut(s) 120
HpyAV CCTTC 3 cut(s) 106, 151, 230
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 1 cut(s) 100
HpyCH4V TGCA 3 cut(s) 224, 266, 310
HpyF3I CTNAG 1 cut(s) 70
HpySE526I ACGT 1 cut(s) 100
Hsp92II CATG 1 cut(s) 36
Kzo9I GATC 3 cut(s) 130, 252, 334
LmnI GCTCC 1 cut(s) 273
LpnPI CCDG 3 cut(s) 75, 163, 284
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 297
MaeII ACGT 1 cut(s) 100
MaeIII GTNAC 1 cut(s) 278
MalI GATC 3 cut(s) 132, 254, 336
MbiI CCGCTC 1 cut(s) 276
MboI GATC 3 cut(s) 130, 252, 334
MboII GAAGA 3 cut(s) 73, 86, 314
MflI RGATCY 1 cut(s) 334
MluCI AATT 1 cut(s) 137
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 2 cut(s) 55, 287
Mph1103I ATGCAT 1 cut(s) 268
MroXI GAANNNNTTC 1 cut(s) 54
MseI TTAA 1 cut(s) 321
NdeII GATC 3 cut(s) 130, 252, 334
NlaIII CATG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 2 cut(s) 11, 169
PkrI GCNGC 1 cut(s) 312
Ple19I CGATCG 1 cut(s) 133
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
PsuI RGATCY 1 cut(s) 334
PvuI CGATCG 1 cut(s) 133
RsaI GTAC 1 cut(s) 259
RsaNI GTAC 1 cut(s) 258
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 3 cut(s) 130, 252, 334
SchI GAGTC 1 cut(s) 125
SetI ASST 1 cut(s) 103
SfaNI GCATC 1 cut(s) 297
SfcI CTRYAG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 1 cut(s) 137
SsiI CCGC 3 cut(s) 240, 276, 289
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 1 cut(s) 103
TaqI TCGA 3 cut(s) 153, 167, 185
TasI AATT 1 cut(s) 137
TfiI GAWTC 2 cut(s) 11, 169
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 129
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 1 cut(s) 162
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 1 cut(s) 129
XapI RAATTY 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.