RLG00000034566
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018079
Physical Location & Seq
Reverse (-)
45056527 .. 45057386
860 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034566

Sequence Viewer

Length: 759 bp
ATGGAGAAAAAACAAGGTGAATTGGAGAAAAAAGTAAGTGATGTATTGAAGGGGCAGCAGGAATCCAATGTTATATCAATGGAGCAGCATGAAGCAATACAAAGACTTGTTCTTAATAGCGTGCAACAACAAGCTCCATCATCAAGCGGAGGAGGAGCTGTTGATCCTACTCTAGGAATAGCATTCCAACAGTTGGTTGATGCTGTCATACAAGAAGAAGTGGAAAAGCTTATTATACTACTAGTGAGTGGTGTAGATCTCGTTCACAAATGCTCTGAGGTTTGCAAGCATGCCAACTACAAAAAGTATGACTTCACAGGAAAATTTTTCAATTTGGAGGTATCTATTTTCAGGCTCTTCATTGAACTCATAGGGCAGGTAGAAAGGACTTTGAAGGAGGCCAGGGATTTTGCAAACAAAATAGATATGGCGAACAAGAAAATTGAGGCTGCATGTTTATGTAACGTGCAGCTGCCTGCAGTTGGATTTGATATACTCAACATGCAGGGACAAAGAGATGTGACTGAGGAGACTCTGTTGGACTCGGCAAGTGTTAAAGAGGAGGGGATCAAACAAATTGAAGGGAGTGATGCAGTACAAAATCAAATGAAGACTGAAGGTTCATCTGTCGTGCTGCTTGAACTTTATTCACCTGTGGATGGATTGGATGTACTGAATATGCAGGGACCATTGGATCTTATTACCACAAATATCCAAGTGGTGGTCACAGAATCAAAAGAAATAAATGCTAGCTGTTGA

Protein Analysis

253

Amino Acids

27.74

Weight (kDa)

4.71

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 70 - 132 2.9e-07 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018079)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g26420
rosa_laevigata RLG00000026791 RLG00000026792 RLG00000034383 RLG00000034390 RLG00000034566
rosa_roxburghii Rroxscaffold_4G00283870 Rroxscaffold_5G00352570
rosa_samantha Rh1AG390300 Rh1AG390400
rosa_wichuraiana Rw1G034490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 367
AccB7I CCANNNNNTGG 2 cut(s) 193, 721
AciI CCGC 1 cut(s) 147
AclWI GGATC 3 cut(s) 158, 575, 702
AcsI RAATTY 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 636
AfaI GTAC 2 cut(s) 597, 672
AfiI CCNNNNNNNGG 5 cut(s) 173, 193, 482, 659, 721
AgsI TTSAA 6 cut(s) 49, 331, 365, 394, 581, 641
AhlI ACTAGT 1 cut(s) 241
AjnI CCWGG 1 cut(s) 401
AluBI AGCT 5 cut(s) 134, 158, 229, 472, 753
AluI AGCT 5 cut(s) 134, 158, 229, 472, 753
Alw26I GTCTC 1 cut(s) 524
AlwI GGATC 3 cut(s) 158, 575, 702
AoxI GGCC 1 cut(s) 399
ApeKI GCWGC 6 cut(s) 55, 85, 449, 469, 472, 634
ApoI RAATTY 1 cut(s) 323
AspS9I GGNCC 1 cut(s) 686
AsuHPI GGTGA 2 cut(s) 29, 642
AsuNHI GCTAGC 1 cut(s) 749
AvaII GGWCC 1 cut(s) 686
BbsI GAAGAC 1 cut(s) 617
BbvI GCAGC 6 cut(s) 67, 97, 436, 459, 481, 621
BccI CCATC 2 cut(s) 145, 653
BciT130I CCWGG 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 524
BcuI ACTAGT 1 cut(s) 241
BfaI CTAG 3 cut(s) 173, 242, 750
BfmI CTRYAG 1 cut(s) 477
BfuAI ACCTGC 1 cut(s) 367
BglII AGATCT 1 cut(s) 256
BisI GCNGC 6 cut(s) 56, 86, 450, 470, 473, 635
BlsI GCNGC 6 cut(s) 57, 87, 451, 471, 474, 636
Bme1390I CCNGG 1 cut(s) 403
Bme18I GGWCC 1 cut(s) 686
BmgT120I GGNCC 1 cut(s) 686
BmiI GGNNCC 1 cut(s) 687
BmrFI CCNGG 1 cut(s) 403
BmsI GCATC 2 cut(s) 190, 580
BmtI GCTAGC 1 cut(s) 753
BpiI GAAGAC 1 cut(s) 617
BsaJI CCNNGG 1 cut(s) 402
BsaXI ACNNNNNCTCC 4 cut(s) 144, 174, 521, 551
Bsc4I CCNNNNNNNGG 5 cut(s) 173, 193, 482, 659, 721
BseBI CCWGG 1 cut(s) 403
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 2 cut(s) 664, 673
BseLI CCNNNNNNNGG 5 cut(s) 173, 193, 482, 659, 721
BseMII CTCAG 2 cut(s) 267, 516
BseRI GAGGAG 4 cut(s) 165, 168, 542, 575
BseXI GCAGC 6 cut(s) 67, 97, 436, 459, 481, 621
BsgI GTGCAG 1 cut(s) 488
BshFI GGCC 1 cut(s) 401
BslFI GGGAC 2 cut(s) 522, 699
BslI CCNNNNNNNGG 5 cut(s) 173, 193, 482, 659, 721
BsmAI GTCTC 1 cut(s) 524
BsmFI GGGAC 2 cut(s) 522, 699
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 1 cut(s) 401
Bsp143I GATC 4 cut(s) 163, 256, 567, 694
BspACI CCGC 1 cut(s) 147
BspANI GGCC 1 cut(s) 401
BspCNI CTCAG 2 cut(s) 268, 517
BspLI GGNNCC 1 cut(s) 687
BspMAI CTGCAG 1 cut(s) 481
BspMI ACCTGC 1 cut(s) 367
BspOI GCTAGC 1 cut(s) 753
BspPI GGATC 3 cut(s) 158, 575, 702
BspQI GCTCTTC 1 cut(s) 362
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 4 cut(s) 163, 256, 567, 694
Bst2UI CCWGG 1 cut(s) 403
Bst4CI ACNGT 1 cut(s) 192
Bst6I CTCTTC 1 cut(s) 362
BstC8I GCNNGC 5 cut(s) 122, 287, 291, 477, 751
BstDEI CTNAG 2 cut(s) 276, 525
BstENI CCTNNNNNAGG 1 cut(s) 171
BstF5I GGATG 2 cut(s) 664, 673
BstKTI GATC 4 cut(s) 166, 259, 570, 697
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 4 cut(s) 163, 256, 567, 694
BstNI CCWGG 1 cut(s) 403
BstNSI RCATGY 3 cut(s) 293, 456, 505
BstSCI CCNGG 1 cut(s) 401
BstSFI CTRYAG 1 cut(s) 477
BstV1I GCAGC 6 cut(s) 67, 97, 436, 459, 481, 621
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 2 cut(s) 256, 694
BstYI RGATCY 2 cut(s) 256, 694
BsuRI GGCC 1 cut(s) 401
BtsCI GGATG 2 cut(s) 664, 673
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 5 cut(s) 122, 287, 291, 477, 751
Cfr13I GGNCC 1 cut(s) 686
Csp6I GTAC 2 cut(s) 596, 671
CviAII CATG 4 cut(s) 89, 290, 453, 502
CviJI RGCY 8 cut(s) 134, 158, 229, 355, 401, 449, 472, 753
CviKI_1 RGCY 8 cut(s) 134, 158, 229, 355, 401, 449, 472, 753
CviQI GTAC 2 cut(s) 596, 671
DdeI CTNAG 2 cut(s) 276, 525
DpnI GATC 4 cut(s) 165, 258, 569, 696
DpnII GATC 4 cut(s) 163, 256, 567, 694
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 686
Eco57I CTGAAG 1 cut(s) 636
EcoNI CCTNNNNNAGG 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 401
FaeI CATG 4 cut(s) 92, 293, 456, 505
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 2 cut(s) 522, 699
FatI CATG 4 cut(s) 88, 289, 452, 501
Fnu4HI GCNGC 6 cut(s) 56, 86, 450, 470, 473, 635
FokI GGATG 2 cut(s) 671, 680
Fsp4HI GCNGC 6 cut(s) 56, 86, 450, 470, 473, 635
FspBI CTAG 3 cut(s) 173, 242, 750
GluI GCNGC 6 cut(s) 56, 86, 450, 470, 473, 635
HaeIII GGCC 1 cut(s) 401
Hin1II CATG 4 cut(s) 92, 293, 456, 505
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 4 cut(s) 62, 532, 542, 731
HphI GGTGA 2 cut(s) 29, 642
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 1 cut(s) 277
Hpy8I GTNNAC 1 cut(s) 265
HpyAV CCTTC 4 cut(s) 43, 388, 575, 611
HpyCH4III ACNGT 1 cut(s) 192
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 9 cut(s) 124, 285, 413, 452, 469, 479, 505, 593, 682
HpyF3I CTNAG 2 cut(s) 276, 525
HpySE526I ACGT 1 cut(s) 465
Hsp92II CATG 4 cut(s) 92, 293, 456, 505
Kzo9I GATC 4 cut(s) 163, 256, 567, 694
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 3 cut(s) 82, 139, 155
Lsp1109I GCAGC 6 cut(s) 67, 97, 436, 459, 481, 621
LweI GCATC 2 cut(s) 190, 580
MaeI CTAG 3 cut(s) 173, 242, 750
MaeII ACGT 1 cut(s) 465
MaeIII GTNAC 3 cut(s) 461, 520, 724
MalI GATC 4 cut(s) 165, 258, 569, 696
MboI GATC 4 cut(s) 163, 256, 567, 694
MboII GAAGA 3 cut(s) 227, 349, 622
MflI RGATCY 2 cut(s) 256, 694
MluCI AATT 5 cut(s) 20, 323, 331, 441, 576
MlyI GAGTC 2 cut(s) 526, 536
MmeI TCCRAC 3 cut(s) 211, 463, 519
MnlI CCTC 9 cut(s) 143, 146, 271, 331, 391, 439, 520, 553, 556
MseI TTAA 2 cut(s) 114, 555
MslI CAYNNNNRTG 1 cut(s) 457
MspA1I CMGCKG 1 cut(s) 472
MspR9I CCNGG 1 cut(s) 403
Mva1269I GAATGC 1 cut(s) 182
MvaI CCWGG 1 cut(s) 403
NdeII GATC 4 cut(s) 163, 256, 567, 694
NheI GCTAGC 1 cut(s) 749
NlaIII CATG 4 cut(s) 92, 293, 456, 505
NlaIV GGNNCC 1 cut(s) 687
NmeAIII GCCGAG 1 cut(s) 524
NmuCI GTSAC 2 cut(s) 520, 724
NspI RCATGY 3 cut(s) 293, 456, 505
PaeI GCATGC 1 cut(s) 293
PciSI GCTCTTC 1 cut(s) 362
PctI GAATGC 1 cut(s) 182
PfeI GAWTC 2 cut(s) 62, 731
PflMI CCANNNNNTGG 2 cut(s) 193, 721
PkrI GCNGC 6 cut(s) 57, 87, 451, 471, 474, 636
PleI GAGTC 2 cut(s) 526, 536
PpsI GAGTC 2 cut(s) 526, 536
Psp6I CCWGG 1 cut(s) 401
PspGI CCWGG 1 cut(s) 401
PspN4I GGNNCC 1 cut(s) 687
PspPI GGNCC 1 cut(s) 686
PsrI GAACNNNNNNTAC 2 cut(s) 246, 278
PstI CTGCAG 1 cut(s) 481
PsuI RGATCY 2 cut(s) 256, 694
PvuII CAGCTG 1 cut(s) 472
RsaI GTAC 2 cut(s) 597, 672
RsaNI GTAC 2 cut(s) 596, 671
RseI CAYNNNNRTG 1 cut(s) 457
SapI GCTCTTC 1 cut(s) 362
SaqAI TTAA 2 cut(s) 114, 555
SatI GCNGC 6 cut(s) 56, 86, 450, 470, 473, 635
Sau3AI GATC 4 cut(s) 163, 256, 567, 694
Sau96I GGNCC 1 cut(s) 686
SchI GAGTC 2 cut(s) 526, 536
ScrFI CCNGG 1 cut(s) 403
SfaNI GCATC 2 cut(s) 190, 580
SfcI CTRYAG 1 cut(s) 477
SinI GGWCC 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 457
SpeI ACTAGT 1 cut(s) 241
SphI GCATGC 1 cut(s) 293
Sse9I AATT 5 cut(s) 20, 323, 331, 441, 576
SsiI CCGC 1 cut(s) 147
SspMI CTAG 3 cut(s) 173, 242, 750
StyD4I CCNGG 1 cut(s) 401
TaaI ACNGT 1 cut(s) 192
TaiI ACGT 1 cut(s) 468
TasI AATT 5 cut(s) 20, 323, 331, 441, 576
TatI WGTACW 2 cut(s) 595, 670
TfiI GAWTC 2 cut(s) 62, 731
Tru1I TTAA 2 cut(s) 114, 555
Tru9I TTAA 2 cut(s) 114, 555
TseFI GTSAC 2 cut(s) 520, 724
TseI GCWGC 6 cut(s) 55, 85, 449, 469, 472, 634
Tsp45I GTSAC 2 cut(s) 520, 724
TspDTI ATGAA 4 cut(s) 105, 349, 612, 623
Van91I CCANNNNNTGG 2 cut(s) 193, 721
VpaK11BI GGWCC 1 cut(s) 686
XagI CCTNNNNNAGG 1 cut(s) 171
XapI RAATTY 1 cut(s) 323
XceI RCATGY 3 cut(s) 293, 456, 505
XspI CTAG 3 cut(s) 173, 242, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.