Rroxscaffold_4G00283870
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018079
Physical Location & Seq
Reverse (-)
5672979 .. 5673983
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283870.1

Sequence Viewer

Length: 1005 bp
ATGACGGTGGGTAAAGCGCTTGTGGAGATGTTATCCAAGCTTAGCATGCGGAACGTCCGCATTTGGGGAAATTGCATAGGGCCTGATTACGCAAAACAAATTAGAGATTTGACTAGGTCTGTTAGAAGCATAGTAAAATCATTAGAATTGGAGCGACAGAGGAAAATGACCGAGCGTGTATCTCTGACAAGAGACGTCAAAAACAAACAAGATGATTTGCAGAAAAAACAAGGTGAACTGTTGAGGAAGATGCAGGAATTGTGGAAAAGAATAGATGAATTATTGGCTAAAGCAGATGGTATATTGAGGGGGCAAGAGGAATCCTATGCTATATTGATGGAGCAGCGTGAAGGAATACAGAGACTTCTTTCTAATAGCGTTGAGCAAGCTCCATCATCAAGCGAGGGAGGGGCTGCTTATCCTGCTCTAGGAGTAGCATTCCGACAGTTGGTTAATGCTGTCACACAAGTGAAAGTCGAGAACAAGTCTTTTAAAGACTTCTTCGACACATTGAAACACACGCTATACTGCTTACAACCAATGATAGAAGAGGTAGCAGAACATGGCAGGGTAGTGCACCTTCCAAAGCAAGAAGTGGAAATGCTTATTATAGAAATAGTGAATGGGGTAGAGCTCGTTCACAAATGTTCCAAGGTTCGGAAGCATACCAACTACAGAAACTATAAATTCACCAACAATATTATCAATTTGAAGGAATCTCTGTTCAGACTCTTCATTGAACTCATGGAACAAGTAGAAAGGACTTTGAAGGAGGCCATGGATTCTACAAACAAAATAGAGATGGTGAACAACAAACTTGAAGAATTATGTCACTTGTTTGTTCGGCAGCTCTCCGAGAGTGTTAGAATTTCTTCCACGGAAAAACCTACCGCTGGCGTCGATTTCAATGTGGACGGTGGAGGAGCGATCGCTGCAGCAGAATTCGAGCCGAGCTTGGTTTGCCTGGCGAAGATGAATCAGAAAGAAACCAACGAGAAACTTTAG

Protein Analysis

334

Amino Acids

38.23

Weight (kDa)

8.65

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 141 - 262 3.6e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018079)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g26420
rosa_laevigata RLG00000026791 RLG00000026792 RLG00000034383 RLG00000034390 RLG00000034566
rosa_roxburghii Rroxscaffold_4G00283870 Rroxscaffold_5G00352570
rosa_samantha Rh1AG390300 Rh1AG390400
rosa_wichuraiana Rw1G034490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
AciI CCGC 3 cut(s) 49, 58, 891
AcsI RAATTY 3 cut(s) 686, 867, 941
AcyI GRCGYC 2 cut(s) 195, 897
AfeI AGCGCT 1 cut(s) 18
AfiI CCNNNNNNNGG 5 cut(s) 64, 428, 448, 657, 893
AgsI TTSAA 6 cut(s) 514, 712, 740, 769, 821, 907
AjnI CCWGG 1 cut(s) 963
AleI CACNNNNGTG 1 cut(s) 467
AloI GAACNNNNNNTCC 2 cut(s) 707, 739
AluBI AGCT 5 cut(s) 40, 389, 634, 850, 954
AluI AGCT 5 cut(s) 40, 389, 634, 850, 954
Alw21I GWGCWC 2 cut(s) 579, 636
Alw26I GTCTC 2 cut(s) 186, 355
Alw44I GTGCAC 1 cut(s) 575
Aor51HI AGCGCT 1 cut(s) 18
AoxI GGCC 2 cut(s) 80, 774
ApaLI GTGCAC 1 cut(s) 575
ApeKI GCWGC 5 cut(s) 343, 413, 847, 932, 935
ApoI RAATTY 3 cut(s) 686, 867, 941
AsiSI GCGATCGC 1 cut(s) 930
Asp700I GAANNNNTTC 1 cut(s) 871
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 80
AsuHPI GGTGA 3 cut(s) 245, 682, 817
BaeGI GKGCMC 1 cut(s) 579
BanII GRGCYC 1 cut(s) 636
BarI GAAGNNNNNNTAC 2 cut(s) 564, 596
Bbv12I GWGCWC 2 cut(s) 579, 636
BbvI GCAGC 5 cut(s) 355, 400, 859, 919, 947
BccI CCATC 4 cut(s) 290, 331, 400, 796
BciT130I CCWGG 1 cut(s) 965
BcoDI GTCTC 2 cut(s) 186, 355
BfaI CTAG 2 cut(s) 114, 428
BfmI CTRYAG 2 cut(s) 673, 933
BfoI RGCGCY 1 cut(s) 20
BisI GCNGC 5 cut(s) 344, 414, 848, 933, 936
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 5 cut(s) 345, 415, 849, 934, 937
Bme1390I CCNGG 1 cut(s) 965
BmgT120I GGNCC 1 cut(s) 80
BmrFI CCNGG 1 cut(s) 965
BmsI GCATC 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 41
BsaHI GRCGYC 2 cut(s) 195, 897
BsaJI CCNNGG 3 cut(s) 651, 777, 876
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 428, 448, 657, 893
BseBI CCWGG 1 cut(s) 965
BseDI CCNNGG 3 cut(s) 651, 777, 876
BseLI CCNNNNNNNGG 5 cut(s) 64, 428, 448, 657, 893
BseRI GAGGAG 1 cut(s) 936
BseSI GKGCMC 1 cut(s) 579
BseXI GCAGC 5 cut(s) 355, 400, 859, 919, 947
Bsh1285I CGRYCG 1 cut(s) 930
BshFI GGCC 2 cut(s) 82, 776
BsiEI CGRYCG 1 cut(s) 930
BsiHKAI GWGCWC 2 cut(s) 579, 636
BslI CCNNNNNNNGG 5 cut(s) 64, 428, 448, 657, 893
BsmAI GTCTC 2 cut(s) 186, 355
BsmBI CGTCTC 1 cut(s) 186
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 2 cut(s) 82, 776
Bsp1286I GDGCHC 2 cut(s) 579, 636
Bsp143I GATC 1 cut(s) 927
Bsp1720I GCTNAGC 1 cut(s) 41
Bsp19I CCATGG 1 cut(s) 777
BspACI CCGC 3 cut(s) 49, 58, 891
BspANI GGCC 2 cut(s) 82, 776
BspMAI CTGCAG 1 cut(s) 937
BssECI CCNNGG 3 cut(s) 651, 777, 876
BssMI GATC 1 cut(s) 927
BssNI GRCGYC 2 cut(s) 195, 897
BssT1I CCWWGG 2 cut(s) 651, 777
Bst2UI CCWGG 1 cut(s) 965
Bst4CI ACNGT 4 cut(s) 7, 240, 447, 917
Bst6I CTCTTC 2 cut(s) 543, 737
BstACI GRCGYC 2 cut(s) 195, 897
BstC8I GCNNGC 3 cut(s) 47, 387, 895
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 2 cut(s) 777, 876
BstENI CCTNNNNNAGG 1 cut(s) 426
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 1 cut(s) 930
BstMAI GTCTC 2 cut(s) 186, 355
BstMBI GATC 1 cut(s) 927
BstMCI CGRYCG 1 cut(s) 930
BstMWI GCNNNNNNNGC 4 cut(s) 46, 422, 932, 960
BstNI CCWGG 1 cut(s) 965
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 963
BstSFI CTRYAG 2 cut(s) 673, 933
BstSLI GKGCMC 1 cut(s) 579
BstV1I GCAGC 5 cut(s) 355, 400, 859, 919, 947
BsuRI GGCC 2 cut(s) 82, 776
BtgI CCRYGG 2 cut(s) 777, 876
Cac8I GCNNGC 3 cut(s) 47, 387, 895
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 1 cut(s) 80
CseI GACGC 1 cut(s) 886
CviAII CATG 4 cut(s) 46, 563, 745, 778
DdeI CTNAG 1 cut(s) 41
DpnI GATC 1 cut(s) 929
DpnII GATC 1 cut(s) 927
DraI TTTAAA 1 cut(s) 493
Eam1104I CTCTTC 2 cut(s) 543, 737
EarI CTCTTC 2 cut(s) 543, 737
Ecl136II GAGCTC 1 cut(s) 634
Eco130I CCWWGG 2 cut(s) 651, 777
Eco24I GRGCYC 1 cut(s) 636
Eco47III AGCGCT 1 cut(s) 18
Eco53kI GAGCTC 1 cut(s) 634
EcoICRI GAGCTC 1 cut(s) 634
EcoNI CCTNNNNNAGG 1 cut(s) 426
EcoO109I RGGNCCY 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 941
EcoRII CCWGG 1 cut(s) 963
EcoT14I CCWWGG 2 cut(s) 651, 777
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 2 cut(s) 651, 777
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 4 cut(s) 49, 566, 748, 781
FatI CATG 4 cut(s) 45, 562, 744, 777
Fnu4HI GCNGC 5 cut(s) 344, 414, 848, 933, 936
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 5 cut(s) 344, 414, 848, 933, 936
FspBI CTAG 2 cut(s) 114, 428
GlaI GCGC 1 cut(s) 18
GluI GCNGC 5 cut(s) 344, 414, 848, 933, 936
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 2 cut(s) 82, 776
HgaI GACGC 1 cut(s) 886
HhaI GCGC 1 cut(s) 19
Hin1I GRCGYC 2 cut(s) 195, 897
Hin1II CATG 4 cut(s) 49, 566, 748, 781
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 5 cut(s) 320, 716, 729, 782, 976
HphI GGTGA 3 cut(s) 245, 682, 817
Hpy166II GTNNAC 5 cut(s) 236, 577, 640, 808, 913
Hpy188I TCNGA 6 cut(s) 186, 443, 660, 728, 856, 981
Hpy188III TCNNGA 1 cut(s) 478
Hpy8I GTNNAC 5 cut(s) 236, 577, 640, 808, 913
Hpy99I CGWCG 1 cut(s) 902
HpyAV CCTTC 4 cut(s) 344, 590, 706, 763
HpyCH4III ACNGT 4 cut(s) 7, 240, 447, 917
HpyCH4IV ACGT 2 cut(s) 54, 195
HpyCH4V TGCA 5 cut(s) 75, 220, 253, 577, 935
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 422, 932, 960
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 2 cut(s) 54, 195
Hsp92I GRCGYC 2 cut(s) 195, 897
Hsp92II CATG 4 cut(s) 49, 566, 748, 781
HspAI GCGC 1 cut(s) 17
Kzo9I GATC 1 cut(s) 927
LmnI GCTCC 4 cut(s) 151, 340, 394, 923
LpnPI CCDG 7 cut(s) 96, 239, 435, 553, 879, 950, 977
Lsp1109I GCAGC 5 cut(s) 355, 400, 859, 919, 947
LweI GCATC 1 cut(s) 240
MaeI CTAG 2 cut(s) 114, 428
MaeII ACGT 2 cut(s) 54, 195
MaeIII GTNAC 2 cut(s) 460, 830
MalI GATC 1 cut(s) 929
MboI GATC 1 cut(s) 927
MboII GAAGA 7 cut(s) 259, 493, 560, 724, 833, 864, 982
MhlI GDGCHC 2 cut(s) 579, 636
MlyI GAGTC 1 cut(s) 723
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 9 cut(s) 153, 237, 300, 310, 397, 401, 544, 766, 914
MroXI GAANNNNTTC 1 cut(s) 871
MseI TTAA 2 cut(s) 453, 492
MslI CAYNNNNRTG 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 893
MspR9I CCNGG 1 cut(s) 965
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 1 cut(s) 965
MwoI GCNNNNNNNGC 4 cut(s) 46, 422, 932, 960
NcoI CCATGG 1 cut(s) 777
NdeII GATC 1 cut(s) 927
NlaIII CATG 4 cut(s) 49, 566, 748, 781
NmeAIII GCCGAG 1 cut(s) 975
NmuCI GTSAC 2 cut(s) 460, 830
NspI RCATGY 1 cut(s) 49
OliI CACNNNNGTG 1 cut(s) 467
PaeI GCATGC 1 cut(s) 49
PctI GAATGC 1 cut(s) 437
PdmI GAANNNNTTC 1 cut(s) 871
PfeI GAWTC 4 cut(s) 320, 716, 782, 976
PflFI GACNNNGTC 1 cut(s) 115
PkrI GCNGC 5 cut(s) 345, 415, 849, 934, 937
Ple19I CGATCG 1 cut(s) 930
PleI GAGTC 1 cut(s) 723
PpsI GAGTC 1 cut(s) 723
Psp124BI GAGCTC 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 963
PspGI CCWGG 1 cut(s) 963
PspPI GGNCC 1 cut(s) 80
PsrI GAACNNNNNNTAC 2 cut(s) 621, 653
PstI CTGCAG 1 cut(s) 937
PsyI GACNNNGTC 1 cut(s) 115
PvuI CGATCG 1 cut(s) 930
RgaI GCGATCGC 1 cut(s) 930
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 636
SaqAI TTAA 2 cut(s) 453, 492
SatI GCNGC 5 cut(s) 344, 414, 848, 933, 936
Sau3AI GATC 1 cut(s) 927
Sau96I GGNCC 1 cut(s) 80
SchI GAGTC 1 cut(s) 723
ScrFI CCNGG 1 cut(s) 965
SduI GDGCHC 2 cut(s) 579, 636
SfaAI GCGATCGC 1 cut(s) 930
SfaNI GCATC 1 cut(s) 240
SfcI CTRYAG 2 cut(s) 673, 933
SgfI GCGATCGC 1 cut(s) 930
SmiMI CAYNNNNRTG 1 cut(s) 467
SphI GCATGC 1 cut(s) 49
SsiI CCGC 3 cut(s) 49, 58, 891
SspI AATATT 1 cut(s) 700
SspMI CTAG 2 cut(s) 114, 428
SstI GAGCTC 1 cut(s) 636
StyD4I CCNGG 1 cut(s) 963
StyI CCWWGG 2 cut(s) 651, 777
TaaI ACNGT 4 cut(s) 7, 240, 447, 917
TaiI ACGT 2 cut(s) 57, 198
TaqI TCGA 4 cut(s) 477, 504, 900, 945
TaqII GACCGA 1 cut(s) 185
TfiI GAWTC 4 cut(s) 320, 716, 782, 976
Tru1I TTAA 2 cut(s) 453, 492
Tru9I TTAA 2 cut(s) 453, 492
TseFI GTSAC 2 cut(s) 460, 830
TseI GCWGC 5 cut(s) 343, 413, 847, 932, 935
Tsp45I GTSAC 2 cut(s) 460, 830
TspDTI ATGAA 3 cut(s) 291, 724, 989
TspGWI ACGGA 1 cut(s) 893
Tth111I GACNNNGTC 1 cut(s) 115
VneI GTGCAC 1 cut(s) 575
XagI CCTNNNNNAGG 1 cut(s) 426
XapI RAATTY 3 cut(s) 686, 867, 941
XceI RCATGY 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 871
XspI CTAG 2 cut(s) 114, 428
ZraI GACGTC 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.