Rw1G034490
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0018079
Physical Location & Seq
Reverse (-)
62394982 .. 62396001
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034490.1

Sequence Viewer

Length: 1020 bp
ATGATTGTCGGTAAAGCGCTTGTGGAGATGTTATCCAAGCTTAGCATGCGGAACGTCCGCATTTGGGGTAATTGCATAGCGCCTGATTACGCAAAACAAATTAAAGATTTTACTAGGTCTGTTAGGAGCCTGGTAAAATCATTAGAATTGGAGCGAGAGAGGAATATGACTGAGCTTGTATCTCTGACAAGAGACGTCAAAAACAAACAAGATGATTTGCAGAAAAAAATGTTGAGGAAGATGCAGGAATTCTGGAAAAGAATAGATGAATTATTGGCTAAAGCAGATGGTATATTGAGGGGGCAAGATGAATCCTATGCTATATTGATGAAGCAGCGTGAAGCAATACAAAGACTTCTTTCTAATAACGTTGAGCAAGCTCCATCATCAAGCGAAGGAGGGGCTGCTTATCCTGCTCTAGGAGTAGCATTCCGACAGTTGGTTAATGCTGTCGCACAAGTGAAAGTCGAGAACAAGTCTTTTAAAGACTTCTTCGACACATTCAAACACACGCTCTACTGCTTACAACCACTGATAGAAGAGGTAGCAGAACATGGCAGGGTAGTGCAGCTTCCAAAGCAAGAAGTGGAAAAGCTTATTATAGAAATAGTGAATGAGGTAGAGCTCGTTGACAAATGCTCCAAGGTTCGGAAGCATACCAACTACAAAAACTATAAATTCACCAACAATATTATCAATTTGAAGGAATCTCTGTTCAGACTCTTCATTGAACTCATGGAACAAGTAGAAAGGACTTTGAAGGAGGCCATGGATTCTACAAACAAAATAGAGATGGTGAACAACAAAATTGAAGAATTATGGCACTTGTTTGTTCGGCAGCTCTCCGAGTGTGTTATAATTTCTTCCACGGAAAAACCTACCGCTGGCGTCGATTTCAATGTGGACAGTGGAGGAGCGATCGCTGCAGCCGAATTCGAGCCGAGCTTGGTTTGCCTGGCGAAGATGAATCATAAAGAAACCAACGAGAAACTTTATATTCAACGAAGTTCATTGCAATGA

Protein Analysis

339

Amino Acids

39.03

Weight (kDa)

8.42

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 138 - 259 1.7e-17 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018079)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g26420
rosa_laevigata RLG00000026791 RLG00000026792 RLG00000034383 RLG00000034390 RLG00000034566
rosa_roxburghii Rroxscaffold_4G00283870 Rroxscaffold_5G00352570
rosa_samantha Rh1AG390300 Rh1AG390400
rosa_wichuraiana Rw1G034490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 857
AatII GACGTC 1 cut(s) 198
AciI CCGC 3 cut(s) 49, 58, 882
AclI AACGTT 1 cut(s) 369
AcsI RAATTY 3 cut(s) 248, 677, 932
AcyI GRCGYC 2 cut(s) 195, 888
AfeI AGCGCT 1 cut(s) 18
AfiI CCNNNNNNNGG 5 cut(s) 64, 419, 439, 648, 884
AgsI TTSAA 7 cut(s) 505, 703, 731, 760, 812, 898, 1001
AjnI CCWGG 2 cut(s) 129, 954
AloI GAACNNNNNNTCC 2 cut(s) 698, 730
AluBI AGCT 8 cut(s) 40, 175, 380, 571, 595, 625, 841, 945
AluI AGCT 8 cut(s) 40, 175, 380, 571, 595, 625, 841, 945
Alw21I GWGCWC 1 cut(s) 627
Alw26I GTCTC 1 cut(s) 186
Aor51HI AGCGCT 1 cut(s) 18
AoxI GGCC 1 cut(s) 765
ApeKI GCWGC 6 cut(s) 334, 404, 568, 838, 923, 926
ApoI RAATTY 3 cut(s) 248, 677, 932
AsiSI GCGATCGC 1 cut(s) 921
AspLEI GCGC 2 cut(s) 19, 82
AsuHPI GGTGA 2 cut(s) 673, 808
BanII GRGCYC 1 cut(s) 627
BarI GAAGNNNNNNTAC 2 cut(s) 555, 587
Bbv12I GWGCWC 1 cut(s) 627
BbvI GCAGC 6 cut(s) 346, 391, 580, 850, 910, 938
BccI CCATC 3 cut(s) 281, 391, 787
BciT130I CCWGG 2 cut(s) 131, 956
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 2 cut(s) 114, 419
BfmI CTRYAG 1 cut(s) 924
BfoI RGCGCY 2 cut(s) 20, 83
BisI GCNGC 6 cut(s) 335, 405, 569, 839, 924, 927
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 6 cut(s) 336, 406, 570, 840, 925, 928
Bme1390I CCNGG 2 cut(s) 131, 956
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 2 cut(s) 131, 956
BmsI GCATC 1 cut(s) 231
Bpu1102I GCTNAGC 1 cut(s) 41
BsaHI GRCGYC 2 cut(s) 195, 888
BsaJI CCNNGG 3 cut(s) 642, 768, 867
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 419, 439, 648, 884
Bse3DI GCAATG 1 cut(s) 1010
BseBI CCWGG 2 cut(s) 131, 956
BseDI CCNNGG 3 cut(s) 642, 768, 867
BseLI CCNNNNNNNGG 5 cut(s) 64, 419, 439, 648, 884
BseMI GCAATG 1 cut(s) 1010
BseMII CTCAG 1 cut(s) 162
BseRI GAGGAG 1 cut(s) 927
BseXI GCAGC 6 cut(s) 346, 391, 580, 850, 910, 938
BsgI GTGCAG 1 cut(s) 587
Bsh1285I CGRYCG 1 cut(s) 921
BshFI GGCC 1 cut(s) 767
BsiEI CGRYCG 1 cut(s) 921
BsiHKAI GWGCWC 1 cut(s) 627
BslI CCNNNNNNNGG 5 cut(s) 64, 419, 439, 648, 884
BsmAI GTCTC 1 cut(s) 186
BsmBI CGTCTC 1 cut(s) 186
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 1 cut(s) 767
Bsp1286I GDGCHC 1 cut(s) 627
Bsp143I GATC 1 cut(s) 918
Bsp1720I GCTNAGC 1 cut(s) 41
Bsp19I CCATGG 1 cut(s) 768
BspACI CCGC 3 cut(s) 49, 58, 882
BspANI GGCC 1 cut(s) 767
BspCNI CTCAG 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 128
BspMAI CTGCAG 1 cut(s) 928
BsrDI GCAATG 1 cut(s) 1010
BssECI CCNNGG 3 cut(s) 642, 768, 867
BssMI GATC 1 cut(s) 918
BssNI GRCGYC 2 cut(s) 195, 888
BssT1I CCWWGG 2 cut(s) 642, 768
Bst2UI CCWGG 2 cut(s) 131, 956
Bst4CI ACNGT 2 cut(s) 438, 908
Bst6I CTCTTC 2 cut(s) 534, 728
BstACI GRCGYC 2 cut(s) 195, 888
BstC8I GCNNGC 3 cut(s) 47, 378, 886
BstDEI CTNAG 2 cut(s) 41, 171
BstDSI CCRYGG 2 cut(s) 768, 867
BstENI CCTNNNNNAGG 1 cut(s) 417
BstH2I RGCGCY 2 cut(s) 20, 83
BstHHI GCGC 2 cut(s) 19, 82
BstKTI GATC 1 cut(s) 921
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 1 cut(s) 918
BstMCI CGRYCG 1 cut(s) 921
BstMWI GCNNNNNNNGC 5 cut(s) 46, 413, 577, 923, 951
BstNI CCWGG 2 cut(s) 131, 956
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 2 cut(s) 129, 954
BstSFI CTRYAG 1 cut(s) 924
BstV1I GCAGC 6 cut(s) 346, 391, 580, 850, 910, 938
BsuRI GGCC 1 cut(s) 767
BtgI CCRYGG 2 cut(s) 768, 867
BtsIMutI CAGTG 2 cut(s) 530, 913
Cac8I GCNNGC 3 cut(s) 47, 378, 886
CfoI GCGC 2 cut(s) 19, 82
CseI GACGC 1 cut(s) 877
CviAII CATG 4 cut(s) 46, 554, 736, 769
DdeI CTNAG 2 cut(s) 41, 171
DpnI GATC 1 cut(s) 920
DpnII GATC 1 cut(s) 918
DraI TTTAAA 1 cut(s) 484
Eam1104I CTCTTC 2 cut(s) 534, 728
EarI CTCTTC 2 cut(s) 534, 728
Ecl136II GAGCTC 1 cut(s) 625
Eco130I CCWWGG 2 cut(s) 642, 768
Eco24I GRGCYC 1 cut(s) 627
Eco47III AGCGCT 1 cut(s) 18
Eco53kI GAGCTC 1 cut(s) 625
EcoICRI GAGCTC 1 cut(s) 625
EcoNI CCTNNNNNAGG 1 cut(s) 417
EcoRI GAATTC 2 cut(s) 248, 932
EcoRII CCWGG 2 cut(s) 129, 954
EcoT14I CCWWGG 2 cut(s) 642, 768
EcoT38I GRGCYC 1 cut(s) 627
ErhI CCWWGG 2 cut(s) 642, 768
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 4 cut(s) 49, 557, 739, 772
FatI CATG 4 cut(s) 45, 553, 735, 768
Fnu4HI GCNGC 6 cut(s) 335, 405, 569, 839, 924, 927
FriOI GRGCYC 1 cut(s) 627
Fsp4HI GCNGC 6 cut(s) 335, 405, 569, 839, 924, 927
FspBI CTAG 2 cut(s) 114, 419
GlaI GCGC 2 cut(s) 18, 81
GluI GCNGC 6 cut(s) 335, 405, 569, 839, 924, 927
HaeII RGCGCY 2 cut(s) 20, 83
HaeIII GGCC 1 cut(s) 767
HgaI GACGC 1 cut(s) 877
HhaI GCGC 2 cut(s) 19, 82
Hin1I GRCGYC 2 cut(s) 195, 888
Hin1II CATG 4 cut(s) 49, 557, 739, 772
Hin6I GCGC 2 cut(s) 17, 80
HinP1I GCGC 2 cut(s) 17, 80
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HindIII AAGCTT 2 cut(s) 38, 593
HinfI GANTC 5 cut(s) 311, 707, 720, 773, 967
HphI GGTGA 2 cut(s) 673, 808
Hpy166II GTNNAC 3 cut(s) 631, 799, 904
Hpy188I TCNGA 5 cut(s) 186, 434, 651, 719, 847
Hpy188III TCNNGA 2 cut(s) 253, 469
Hpy8I GTNNAC 3 cut(s) 631, 799, 904
Hpy99I CGWCG 1 cut(s) 893
HpyAV CCTTC 3 cut(s) 389, 697, 754
HpyCH4III ACNGT 2 cut(s) 438, 908
HpyCH4IV ACGT 3 cut(s) 54, 195, 369
HpyCH4V TGCA 6 cut(s) 75, 220, 244, 568, 926, 1015
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 413, 577, 923, 951
HpyF3I CTNAG 2 cut(s) 41, 171
HpySE526I ACGT 3 cut(s) 54, 195, 369
Hsp92I GRCGYC 2 cut(s) 195, 888
Hsp92II CATG 4 cut(s) 49, 557, 739, 772
HspAI GCGC 2 cut(s) 17, 80
Kzo9I GATC 1 cut(s) 918
LmnI GCTCC 5 cut(s) 126, 151, 385, 644, 914
Lsp1109I GCAGC 6 cut(s) 346, 391, 580, 850, 910, 938
LweI GCATC 1 cut(s) 231
MaeI CTAG 2 cut(s) 114, 419
MaeII ACGT 3 cut(s) 54, 195, 369
MalI GATC 1 cut(s) 920
MboI GATC 1 cut(s) 918
MboII GAAGA 7 cut(s) 250, 484, 551, 715, 824, 855, 973
MhlI GDGCHC 1 cut(s) 627
MlyI GAGTC 1 cut(s) 714
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 8 cut(s) 153, 228, 291, 392, 535, 610, 757, 905
MseI TTAA 3 cut(s) 102, 444, 483
MslI CAYNNNNRTG 1 cut(s) 1015
MspA1I CMGCKG 1 cut(s) 884
MspR9I CCNGG 2 cut(s) 131, 956
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 2 cut(s) 131, 956
MwoI GCNNNNNNNGC 5 cut(s) 46, 413, 577, 923, 951
NcoI CCATGG 1 cut(s) 768
NdeII GATC 1 cut(s) 918
NlaIII CATG 4 cut(s) 49, 557, 739, 772
NlaIV GGNNCC 1 cut(s) 128
NmeAIII GCCGAG 1 cut(s) 966
NspI RCATGY 1 cut(s) 49
PaeI GCATGC 1 cut(s) 49
PcsI WCGNNNNNNNCGW 1 cut(s) 927
PctI GAATGC 1 cut(s) 428
PfeI GAWTC 4 cut(s) 311, 707, 773, 967
PkrI GCNGC 6 cut(s) 336, 406, 570, 840, 925, 928
Ple19I CGATCG 1 cut(s) 921
PleI GAGTC 1 cut(s) 714
PpsI GAGTC 1 cut(s) 714
PsiI TTATAA 1 cut(s) 857
Psp124BI GAGCTC 1 cut(s) 627
Psp1406I AACGTT 1 cut(s) 369
Psp6I CCWGG 2 cut(s) 129, 954
PspGI CCWGG 2 cut(s) 129, 954
PspN4I GGNNCC 1 cut(s) 128
PstI CTGCAG 1 cut(s) 928
PvuI CGATCG 1 cut(s) 921
RgaI GCGATCGC 1 cut(s) 921
RseI CAYNNNNRTG 1 cut(s) 1015
SacI GAGCTC 1 cut(s) 627
SaqAI TTAA 3 cut(s) 102, 444, 483
SatI GCNGC 6 cut(s) 335, 405, 569, 839, 924, 927
Sau3AI GATC 1 cut(s) 918
SchI GAGTC 1 cut(s) 714
ScrFI CCNGG 2 cut(s) 131, 956
SduI GDGCHC 1 cut(s) 627
SfaAI GCGATCGC 1 cut(s) 921
SfaNI GCATC 1 cut(s) 231
SfcI CTRYAG 1 cut(s) 924
SgfI GCGATCGC 1 cut(s) 921
SmiMI CAYNNNNRTG 1 cut(s) 1015
SphI GCATGC 1 cut(s) 49
SsiI CCGC 3 cut(s) 49, 58, 882
SspI AATATT 1 cut(s) 691
SspMI CTAG 2 cut(s) 114, 419
SstI GAGCTC 1 cut(s) 627
StyD4I CCNGG 2 cut(s) 129, 954
StyI CCWWGG 2 cut(s) 642, 768
TaaI ACNGT 2 cut(s) 438, 908
TaiI ACGT 3 cut(s) 57, 198, 372
TaqI TCGA 4 cut(s) 468, 495, 891, 936
TfiI GAWTC 4 cut(s) 311, 707, 773, 967
Tru1I TTAA 3 cut(s) 102, 444, 483
Tru9I TTAA 3 cut(s) 102, 444, 483
TscAI CASTG 2 cut(s) 537, 913
TseI GCWGC 6 cut(s) 334, 404, 568, 838, 923, 926
TspDTI ATGAA 6 cut(s) 282, 324, 344, 715, 980, 999
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 2 cut(s) 537, 913
XagI CCTNNNNNAGG 1 cut(s) 417
XapI RAATTY 3 cut(s) 248, 677, 932
XceI RCATGY 1 cut(s) 49
XspI CTAG 2 cut(s) 114, 419
ZraI GACGTC 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.