RLG00000035719

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004646
Physical Location & Seq
Reverse (-)
70794961 .. 70795527
567 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035719

Sequence Viewer

Length: 432 bp
ATGAATCCATTTTTCACCAATAATTTTCAGGCACGCAAGGCTCAGATTGAAGATCTTGAACCACCACAAGTGTCTGAAACGCTTGTGGGGTTGGTACAAGAAGAGCCAATGGAGTTCAATGCAGCTCAGTTACGCAATGATGATCAGTTAGCCGGTATGACAGAGCTAGCTGATCATCAGCCACCATTGACAAAGCGTGGCCGCGGACGACCAAGGAAGGTTGACGGGTGTGGGGGCAGCAGCAGTGGACGGCTTAGGCCACAGGAACGAAGATCACAGGAGCGGTTGCTTGAAGGATTGCCCTCTGATTGGCAGGTGATAAAAAAAACCCGAAAGAGTGGCAAATCTGCCGGGCAGGTTGATAAGGTTAGGCGCCTATCAATGAGATTTCTTAACCATATATGCAATTTATTTCCTGAATTTATTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

16.32

Weight (kDa)

9.56

Isoelectric Point (pI)

53.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 304
Acc36I ACCTGC 2 cut(s) 304, 346
AccB1I GGYRCC 1 cut(s) 372
AccBSI CCGCTC 1 cut(s) 283
AccII CGCG 1 cut(s) 204
AciI CCGC 3 cut(s) 202, 204, 283
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 1 cut(s) 419
AcyI GRCGYC 1 cut(s) 373
AfaI GTAC 1 cut(s) 96
AfiI CCNNNNNNNGG 1 cut(s) 309
AgsI TTSAA 4 cut(s) 50, 59, 118, 293
AluBI AGCT 3 cut(s) 125, 166, 170
AluI AGCT 3 cut(s) 125, 166, 170
AoxI GGCC 2 cut(s) 199, 257
ApeKI GCWGC 3 cut(s) 122, 237, 240
ApoI RAATTY 1 cut(s) 419
AspLEI GCGC 1 cut(s) 375
AsuC2I CCSGG 1 cut(s) 352
AsuHPI GGTGA 2 cut(s) 7, 328
AsuNHI GCTAGC 1 cut(s) 166
BanI GGYRCC 1 cut(s) 372
BbvI GCAGC 3 cut(s) 134, 249, 252
BceAI ACGGC 1 cut(s) 266
BclI TGATCA 2 cut(s) 142, 172
BcnI CCSGG 1 cut(s) 352
BfaI CTAG 1 cut(s) 167
BfoI RGCGCY 1 cut(s) 376
BfuAI ACCTGC 2 cut(s) 304, 346
BglII AGATCT 1 cut(s) 52
BisI GCNGC 4 cut(s) 123, 202, 238, 241
BlsI GCNGC 4 cut(s) 124, 203, 239, 242
Bme1390I CCNGG 1 cut(s) 352
BmiI GGNNCC 1 cut(s) 374
BmrFI CCNGG 1 cut(s) 352
BmtI GCTAGC 1 cut(s) 170
Bpu10I CCTNAGC 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 352
BsaHI GRCGYC 1 cut(s) 373
BsaJI CCNNGG 2 cut(s) 202, 212
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse118I RCCGGY 1 cut(s) 152
Bse3DI GCAATG 1 cut(s) 142
BseDI CCNNGG 2 cut(s) 202, 212
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 2 cut(s) 56, 140
BseXI GCAGC 3 cut(s) 134, 249, 252
Bsh1236I CGCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 201, 259
BshNI GGYRCC 1 cut(s) 372
BsiSI CCGG 2 cut(s) 153, 351
BslI CCNNNNNNNGG 1 cut(s) 309
BsnI GGCC 2 cut(s) 201, 259
Bsp143I GATC 4 cut(s) 52, 142, 172, 272
BspACI CCGC 3 cut(s) 202, 204, 283
BspANI GGCC 2 cut(s) 201, 259
BspCNI CTCAG 2 cut(s) 55, 139
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 374
BspMI ACCTGC 2 cut(s) 304, 346
BspOI GCTAGC 1 cut(s) 170
BspQI GCTCTTC 1 cut(s) 96
BspT107I GGYRCC 1 cut(s) 372
BsrBI CCGCTC 1 cut(s) 283
BsrDI GCAATG 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 152
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 202, 212
BssMI GATC 4 cut(s) 52, 142, 172, 272
BssNI GRCGYC 1 cut(s) 373
BssT1I CCWWGG 1 cut(s) 212
Bst6I CTCTTC 1 cut(s) 96
BstACI GRCGYC 1 cut(s) 373
BstC8I GCNNGC 2 cut(s) 34, 168
BstDEI CTNAG 3 cut(s) 42, 126, 254
BstDSI CCRYGG 1 cut(s) 202
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 4 cut(s) 55, 145, 175, 275
BstMBI GATC 4 cut(s) 52, 142, 172, 272
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 350
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 3 cut(s) 134, 249, 252
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
BsuRI GGCC 2 cut(s) 201, 259
BtgI CCRYGG 1 cut(s) 202
BtsI GCAGTG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 250
BveI ACCTGC 2 cut(s) 304, 346
Cac8I GCNNGC 2 cut(s) 34, 168
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 152
Cfr42I CCGCGG 1 cut(s) 205
Csp6I GTAC 1 cut(s) 95
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 3 cut(s) 42, 126, 254
DinI GGCGCC 1 cut(s) 374
DpnI GATC 4 cut(s) 54, 144, 174, 274
DpnII GATC 4 cut(s) 52, 142, 172, 272
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 212
EgeI GGCGCC 1 cut(s) 374
EheI GGCGCC 1 cut(s) 374
ErhI CCWWGG 1 cut(s) 212
FaiI YATR 4 cut(s) 158, 399, 401, 403
FbaI TGATCA 2 cut(s) 142, 172
Fnu4HI GCNGC 4 cut(s) 123, 202, 238, 241
Fsp4HI GCNGC 4 cut(s) 123, 202, 238, 241
FspBI CTAG 1 cut(s) 167
GlaI GCGC 1 cut(s) 374
GluI GCNGC 4 cut(s) 123, 202, 238, 241
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 2 cut(s) 201, 259
HapII CCGG 2 cut(s) 153, 351
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 1 cut(s) 373
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HinfI GANTC 1 cut(s) 4
HpaII CCGG 2 cut(s) 153, 351
HphI GGTGA 2 cut(s) 7, 328
Hpy166II GTNNAC 2 cut(s) 223, 248
Hpy188I TCNGA 3 cut(s) 45, 76, 307
Hpy188III TCNNGA 2 cut(s) 56, 416
Hpy8I GTNNAC 2 cut(s) 223, 248
HpyAV CCTTC 2 cut(s) 211, 287
HpyCH4V TGCA 2 cut(s) 122, 405
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 3 cut(s) 42, 126, 254
Hsp92I GRCGYC 1 cut(s) 373
HspAI GCGC 1 cut(s) 373
KasI GGCGCC 1 cut(s) 372
Ksp22I TGATCA 2 cut(s) 142, 172
KspI CCGCGG 1 cut(s) 205
Kzo9I GATC 4 cut(s) 52, 142, 172, 272
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 7 cut(s) 14, 166, 248, 263, 299, 341, 364
Lsp1109I GCAGC 3 cut(s) 134, 249, 252
MaeI CTAG 1 cut(s) 167
MaeIII GTNAC 1 cut(s) 129
MalI GATC 4 cut(s) 54, 144, 174, 274
MbiI CCGCTC 1 cut(s) 283
MboI GATC 4 cut(s) 52, 142, 172, 272
MboII GAAGA 3 cut(s) 62, 113, 282
MflI RGATCY 1 cut(s) 52
MluCI AATT 3 cut(s) 22, 406, 419
Mly113I GGCGCC 1 cut(s) 373
MnlI CCTC 1 cut(s) 313
MseI TTAA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 204
MspI CCGG 2 cut(s) 153, 351
MspR9I CCNGG 1 cut(s) 352
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 1 cut(s) 38
NarI GGCGCC 1 cut(s) 373
NciI CCSGG 1 cut(s) 352
NdeII GATC 4 cut(s) 52, 142, 172, 272
NheI GCTAGC 1 cut(s) 166
NlaIV GGNNCC 1 cut(s) 374
PaqCI CACCTGC 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 96
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 4 cut(s) 124, 203, 239, 242
PluTI GGCGCC 1 cut(s) 376
PspN4I GGNNCC 1 cut(s) 374
PsuI RGATCY 1 cut(s) 52
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
SacII CCGCGG 1 cut(s) 205
SapI GCTCTTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 4 cut(s) 123, 202, 238, 241
Sau3AI GATC 4 cut(s) 52, 142, 172, 272
ScrFI CCNGG 1 cut(s) 352
SetI ASST 7 cut(s) 127, 168, 172, 222, 318, 360, 369
SfoI GGCGCC 1 cut(s) 374
Sfr303I CCGCGG 1 cut(s) 205
SgrBI CCGCGG 1 cut(s) 205
Sse9I AATT 3 cut(s) 22, 406, 419
SsiI CCGC 3 cut(s) 202, 204, 283
SspDI GGCGCC 1 cut(s) 372
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 350
StyI CCWWGG 1 cut(s) 212
TasI AATT 3 cut(s) 22, 406, 419
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 1 cut(s) 250
TseI GCWGC 3 cut(s) 122, 237, 240
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 250
XapI RAATTY 1 cut(s) 419
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.