Rmu_co8319255.1_g000001

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8319255.1
OG0007243
Physical Location & Seq
Forward (+)
445 .. 942
498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8319255.1_g000001.1.cds

Sequence Viewer

Length: 498 bp
atgggtattccattagagctttcttctcttagctgtggactgagaatcatacaagccaaaaacatagagttcatcaagaccacgggaaacctgttcgttagatactatctttctgcaggggacagcaaaaaaattaggctgaacactcgagaaatctccaccaagtctgaccacatctggaacgagtccatttccttggagtgtaatggaagcgacagctttgtgcgagaaactgtggtttttgagctccggtggaggaacaccgtgcctgtttttggaagaattgggggatcacaactactgggcagggcagaggtcccatggaaagaagtcctcgagtcaccaaacatggaattggacaagtgggtcacgatggttccaagaagtactagtggccgtgctattgaaggcgtcaagccgcctaaactacaggtatcgatcaaagttggagtccaagcagaagtgggaatggagaaaaggaggcagagaaggacgaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

166

Amino Acids

18.79

Weight (kDa)

9.89

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 365
AciI CCGC 1 cut(s) 419
AclWI GGATC 1 cut(s) 298
AcoI YGGCCR 1 cut(s) 394
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 1 cut(s) 275
AgsI TTSAA 1 cut(s) 407
AhlI ACTAGT 1 cut(s) 389
AluBI AGCT 4 cut(s) 19, 33, 219, 247
AluI AGCT 4 cut(s) 19, 33, 219, 247
Alw21I GWGCWC 1 cut(s) 249
AlwI GGATC 1 cut(s) 298
Ama87I CYCGRG 2 cut(s) 147, 335
AoxI GGCC 1 cut(s) 394
ArsI GACNNNNNNTTYG 2 cut(s) 435, 467
AspS9I GGNCC 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 333
AvaI CYCGRG 2 cut(s) 147, 335
AvaII GGWCC 1 cut(s) 316
BanII GRGCYC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 249
BccI CCATC 1 cut(s) 367
BceAI ACGGC 1 cut(s) 381
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 2 cut(s) 114, 428
BisI GCNGC 1 cut(s) 419
BlsI GCNGC 1 cut(s) 420
BmcAI AGTACT 1 cut(s) 388
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 2 cut(s) 147, 335
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 2 cut(s) 318, 378
BmrI ACTGGG 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 81, 195, 320
BsaWI WCCGGW 1 cut(s) 249
Bsc4I CCNNNNNNNGG 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 306
BseCI ATCGAT 1 cut(s) 437
BseDI CCNNGG 3 cut(s) 81, 195, 320
BseLI CCNNNNNNNGG 1 cut(s) 275
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 306
BshFI GGCC 1 cut(s) 396
BshVI ATCGAT 1 cut(s) 437
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 2 cut(s) 147, 335
BsiSI CCGG 1 cut(s) 250
BslFI GGGAC 2 cut(s) 134, 302
BslI CCNNNNNNNGG 1 cut(s) 275
BsmFI GGGAC 2 cut(s) 134, 302
BsnI GGCC 1 cut(s) 396
BsoBI CYCGRG 2 cut(s) 147, 335
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 2 cut(s) 290, 438
Bsp19I CCATGG 1 cut(s) 320
BspACI CCGC 1 cut(s) 419
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 1 cut(s) 33
BspDI ATCGAT 1 cut(s) 437
BspLI GGNNCC 2 cut(s) 318, 378
BspMAI CTGCAG 1 cut(s) 118
BspPI GGATC 1 cut(s) 298
BsrI ACTGG 1 cut(s) 306
BssECI CCNNGG 3 cut(s) 81, 195, 320
BssMI GATC 2 cut(s) 290, 438
BssNI GRCGYC 1 cut(s) 411
BssT1I CCWWGG 2 cut(s) 195, 320
Bst4CI ACNGT 2 cut(s) 235, 265
BstACI GRCGYC 1 cut(s) 411
BstDEI CTNAG 2 cut(s) 29, 41
BstDSI CCRYGG 2 cut(s) 81, 320
BstKTI GATC 2 cut(s) 293, 441
BstMBI GATC 2 cut(s) 290, 438
BstSFI CTRYAG 2 cut(s) 114, 428
BstXI CCANNNNNNTGG 1 cut(s) 196
Bsu15I ATCGAT 1 cut(s) 437
BsuRI GGCC 1 cut(s) 396
BsuTUI ATCGAT 1 cut(s) 437
BtgI CCRYGG 2 cut(s) 81, 320
Cfr13I GGNCC 1 cut(s) 316
ClaI ATCGAT 1 cut(s) 437
CseI GACGC 1 cut(s) 400
Csp6I GTAC 1 cut(s) 387
CviAII CATG 2 cut(s) 321, 349
CviJI RGCY 8 cut(s) 19, 33, 56, 139, 219, 247, 396, 418
CviKI_1 RGCY 8 cut(s) 19, 33, 56, 139, 219, 247, 396, 418
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 2 cut(s) 29, 41
DpnI GATC 2 cut(s) 292, 440
DpnII GATC 2 cut(s) 290, 438
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
EaeI YGGCCR 1 cut(s) 394
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 2 cut(s) 195, 320
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 247
Eco88I CYCGRG 2 cut(s) 147, 335
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 316
EcoT14I CCWWGG 2 cut(s) 195, 320
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 2 cut(s) 195, 320
FaeI CATG 2 cut(s) 324, 352
FaiI YATR 4 cut(s) 50, 65, 322, 350
FaqI GGGAC 2 cut(s) 134, 302
FatI CATG 2 cut(s) 320, 348
Fnu4HI GCNGC 1 cut(s) 419
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 419
FspBI CTAG 1 cut(s) 390
GluI GCNGC 1 cut(s) 419
HaeIII GGCC 1 cut(s) 396
HapII CCGG 1 cut(s) 250
HgaI GACGC 1 cut(s) 400
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 2 cut(s) 324, 352
HinfI GANTC 4 cut(s) 45, 185, 338, 450
HpaII CCGG 1 cut(s) 250
HphI GGTGA 1 cut(s) 333
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 4 cut(s) 76, 149, 178, 370
Hpy8I GTNNAC 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 401, 483
HpyCH4III ACNGT 2 cut(s) 235, 265
HpyCH4V TGCA 1 cut(s) 116
HpyF3I CTNAG 2 cut(s) 29, 41
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 2 cut(s) 324, 352
Kzo9I GATC 2 cut(s) 290, 438
LmnI GCTCC 1 cut(s) 252
LpnPI CCDG 8 cut(s) 102, 104, 163, 263, 282, 287, 292, 416
MaeI CTAG 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 339, 367
MalI GATC 2 cut(s) 292, 440
MboI GATC 2 cut(s) 290, 438
MboII GAAGA 2 cut(s) 15, 291
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 3 cut(s) 132, 282, 353
MlyI GAGTC 3 cut(s) 194, 347, 459
MmeI TCCRAC 1 cut(s) 427
MnlI CCTC 4 cut(s) 249, 307, 344, 474
MspI CCGG 1 cut(s) 250
NcoI CCATGG 1 cut(s) 320
NdeII GATC 2 cut(s) 290, 438
NlaIII CATG 2 cut(s) 324, 352
NlaIV GGNNCC 2 cut(s) 318, 378
NmuCI GTSAC 2 cut(s) 339, 367
PaeR7I CTCGAG 2 cut(s) 147, 335
PfeI GAWTC 1 cut(s) 45
PkrI GCNGC 1 cut(s) 420
PleI GAGTC 3 cut(s) 193, 346, 458
PpsI GAGTC 3 cut(s) 193, 346, 458
PpuMI RGGWCCY 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 316
PspN4I GGNNCC 2 cut(s) 318, 378
PspPI GGNCC 1 cut(s) 316
PspPPI RGGWCCY 1 cut(s) 316
PspXI VCTCGAGB 1 cut(s) 335
PstI CTGCAG 1 cut(s) 118
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
SacI GAGCTC 1 cut(s) 249
SatI GCNGC 1 cut(s) 419
Sau3AI GATC 2 cut(s) 290, 438
Sau96I GGNCC 1 cut(s) 316
ScaI AGTACT 1 cut(s) 388
SchI GAGTC 3 cut(s) 194, 347, 459
SduI GDGCHC 1 cut(s) 249
SetI ASST 7 cut(s) 21, 35, 93, 221, 249, 318, 435
SfcI CTRYAG 2 cut(s) 114, 428
Sfr274I CTCGAG 2 cut(s) 147, 335
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 2 cut(s) 147, 335
SmlI CTYRAG 2 cut(s) 147, 335
SmoI CTYRAG 2 cut(s) 147, 335
SpeI ACTAGT 1 cut(s) 389
Sse9I AATT 3 cut(s) 132, 282, 353
SsiI CCGC 1 cut(s) 419
SspMI CTAG 1 cut(s) 390
SstI GAGCTC 1 cut(s) 249
StyI CCWWGG 2 cut(s) 195, 320
TaaI ACNGT 2 cut(s) 235, 265
TaqI TCGA 3 cut(s) 148, 336, 437
TasI AATT 3 cut(s) 132, 282, 353
TatI WGTACW 1 cut(s) 386
TauI GCSGC 1 cut(s) 421
TfiI GAWTC 1 cut(s) 45
TseFI GTSAC 2 cut(s) 339, 367
Tsp45I GTSAC 2 cut(s) 339, 367
TspDTI ATGAA 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 316
XhoI CTCGAG 2 cut(s) 147, 335
XspI CTAG 1 cut(s) 390
ZrmI AGTACT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.