Rmu_sc0000063.1_g000037

LRR receptor-like serine threonine-protein kinase At4g08850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000063.1
OG0017734
Physical Location & Seq
Reverse (-)
179236 .. 180495
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1260 bp
atggagataggaaagttgaaatcattggtgaatctaagcttaagcaacaatcacctcagtggttcagttccgtcaacactaggggatcttacgaaccttaccactctctatctccatataaataatctttctggcactattcccatggagataggaaagttgaaatcattggtgaatctaagcttaagcaacaatcacctcagtggttcagttccgtcaacactaggggatcttacgaaccttaccactctctatctccatataaataatctttctggcactattcccatggagataggaaagttgaaatcattggtgaatctaagcttaagcaacaatcacctcagtggttcagttccgtcaacactaggggatcttacgaaccttaccactctctatctccatataaataatctttctggcactattcccatggagataggaaagttgaaatcattggtgaatctaagcttaagcaacaatcacctcagtggttcagttccgtcaacactaggggatcttacgaaccttaccactctctatctccatataaataatctttctggcactattcccatggagataggaaagttgaaatcattggtgaatctaagcttaagcaacaatcacctcagtggttcagttccgtcaacactaggggatcttacgaaccttaccactctctatctccatataaataatctttctggcactattcccatggagataggaaagttgaaatcattggtgaatctaagcttaagcaacaatcacctcagtggttcagttccgtcaacactaggggatcttacgaaccttaccactctctatctccatataaataatctttctggcactattcccatggagataggaaacctgaaatcattggtgaatctaagcttcagcaccaatcaactcagtggttcaattccgataacactgggggatctgaccaaccttaccactctctatctctttgaaaataatctttctggccctattcctatggagataggaaacttgaaatcattggtgaatctattcttgagcaccaatcaactcaagggttcaattccgacaacactgggggatctgaccaaccttaccattctctccctccattcaaataatctttctggcactattcctatggagataggaaacctgaaatcattggtggatttacggttgaagttcagttccgtcaacactaggggatcttacgaaccttaccactctctatctccatataaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase RVT_2

Protein Analysis

419

Amino Acids

44.68

Weight (kDa)

7.27

Isoelectric Point (pI)

15.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 2 - 89 1.3e-09 Leucine-rich repeat region
LRR_4 PF12799 7 - 47 7.6e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 8 - 67 6.4e-06 Leucine rich repeat
LRR_14 PF23598 37 - 137 5.6e-12 Leucine-rich repeat region
LRR_4 PF12799 55 - 95 7.6e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 56 - 115 5.9e-06 Leucine rich repeat
LRR_14 PF23598 85 - 185 8.1e-12 Leucine-rich repeat region
LRR_4 PF12799 103 - 143 7.6e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 104 - 163 6.9e-06 Leucine rich repeat
LRR_14 PF23598 133 - 233 4.1e-12 Leucine-rich repeat region
LRR_4 PF12799 151 - 191 7.6e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 152 - 211 5.5e-06 Leucine rich repeat
LRR_4 PF12799 199 - 239 7.6e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 200 - 259 5.8e-06 Leucine rich repeat
LRR_14 PF23598 229 - 326 5.7e-12 Leucine-rich repeat region
LRR_4 PF12799 247 - 287 5.7e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 334 - 400 2.5e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 9 cut(s) 93, 237, 381, 525, 669, 813, 957, 1101, 1228
AcuI CTGAAG 1 cut(s) 889
AflII CTTAAG 6 cut(s) 40, 184, 328, 472, 616, 760
AleI CACNNNNGTG 6 cut(s) 57, 201, 345, 489, 633, 777
AluBI AGCT 7 cut(s) 39, 183, 327, 471, 615, 759, 903
AluI AGCT 7 cut(s) 39, 183, 327, 471, 615, 759, 903
Alw21I GWGCWC 1 cut(s) 1055
AlwI GGATC 9 cut(s) 93, 237, 381, 525, 669, 813, 957, 1101, 1228
AoxI GGCC 1 cut(s) 997
Asp700I GAANNNNTTC 1 cut(s) 1043
AspS9I GGNCC 1 cut(s) 998
Bbv12I GWGCWC 1 cut(s) 1055
BfaI CTAG 7 cut(s) 80, 224, 368, 512, 656, 800, 1215
BfrI CTTAAG 6 cut(s) 40, 184, 328, 472, 616, 760
BmgT120I GGNCC 1 cut(s) 998
BmrI ACTGGG 2 cut(s) 953, 1097
BmuI ACTGGG 2 cut(s) 953, 1097
BpuEI CTTGAG 2 cut(s) 1049, 1069
BsaJI CCNNGG 6 cut(s) 144, 288, 432, 576, 720, 864
Bse1I ACTGG 2 cut(s) 948, 1092
BseDI CCNNGG 6 cut(s) 144, 288, 432, 576, 720, 864
BseMII CTCAG 7 cut(s) 70, 214, 358, 502, 646, 790, 934
BseNI ACTGG 2 cut(s) 948, 1092
BshFI GGCC 1 cut(s) 999
BsiHKAI GWGCWC 1 cut(s) 1055
BsnI GGCC 1 cut(s) 999
Bsp1286I GDGCHC 1 cut(s) 1055
Bsp143I GATC 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
Bsp19I CCATGG 6 cut(s) 144, 288, 432, 576, 720, 864
BspANI GGCC 1 cut(s) 999
BspCNI CTCAG 7 cut(s) 69, 213, 357, 501, 645, 789, 933
BspPI GGATC 9 cut(s) 93, 237, 381, 525, 669, 813, 957, 1101, 1228
BspTI CTTAAG 6 cut(s) 40, 184, 328, 472, 616, 760
BsrI ACTGG 2 cut(s) 948, 1092
BssECI CCNNGG 6 cut(s) 144, 288, 432, 576, 720, 864
BssMI GATC 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
BssT1I CCWWGG 6 cut(s) 144, 288, 432, 576, 720, 864
Bst4CI ACNGT 1 cut(s) 1191
BstAFI CTTAAG 6 cut(s) 40, 184, 328, 472, 616, 760
BstDSI CCRYGG 6 cut(s) 144, 288, 432, 576, 720, 864
BstKTI GATC 9 cut(s) 88, 232, 376, 520, 664, 808, 952, 1096, 1223
BstMBI GATC 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
BstX2I RGATCY 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
BstYI RGATCY 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
BsuRI GGCC 1 cut(s) 999
BtgI CCRYGG 6 cut(s) 144, 288, 432, 576, 720, 864
BtsIMutI CAGTG 9 cut(s) 64, 208, 352, 496, 640, 784, 928, 941, 1085
Cfr13I GGNCC 1 cut(s) 998
CviAII CATG 6 cut(s) 145, 289, 433, 577, 721, 865
CviJI RGCY 8 cut(s) 39, 183, 327, 471, 615, 759, 903, 999
CviKI_1 RGCY 8 cut(s) 39, 183, 327, 471, 615, 759, 903, 999
DpnI GATC 9 cut(s) 87, 231, 375, 519, 663, 807, 951, 1095, 1222
DpnII GATC 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
Eco130I CCWWGG 6 cut(s) 144, 288, 432, 576, 720, 864
Eco57I CTGAAG 1 cut(s) 889
EcoT14I CCWWGG 6 cut(s) 144, 288, 432, 576, 720, 864
ErhI CCWWGG 6 cut(s) 144, 288, 432, 576, 720, 864
FaeI CATG 6 cut(s) 148, 292, 436, 580, 724, 868
FatI CATG 6 cut(s) 144, 288, 432, 576, 720, 864
FspBI CTAG 7 cut(s) 80, 224, 368, 512, 656, 800, 1215
HaeIII GGCC 1 cut(s) 999
Hin1II CATG 6 cut(s) 148, 292, 436, 580, 724, 868
HincII GTYRAC 7 cut(s) 75, 219, 363, 507, 651, 795, 1210
HindII GTYRAC 7 cut(s) 75, 219, 363, 507, 651, 795, 1210
HindIII AAGCTT 7 cut(s) 37, 181, 325, 469, 613, 757, 901
HinfI GANTC 8 cut(s) 31, 175, 319, 463, 607, 751, 895, 1039
Hpy166II GTNNAC 7 cut(s) 75, 219, 363, 507, 651, 795, 1210
Hpy188I TCNGA 4 cut(s) 936, 954, 1080, 1098
Hpy188III TCNNGA 1 cut(s) 1048
Hpy8I GTNNAC 7 cut(s) 75, 219, 363, 507, 651, 795, 1210
HpyCH4III ACNGT 1 cut(s) 1191
Hsp92II CATG 6 cut(s) 148, 292, 436, 580, 724, 868
Kzo9I GATC 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
MaeI CTAG 7 cut(s) 80, 224, 368, 512, 656, 800, 1215
MalI GATC 9 cut(s) 87, 231, 375, 519, 663, 807, 951, 1095, 1222
MboI GATC 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
MflI RGATCY 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
MhlI GDGCHC 1 cut(s) 1055
MluCI AATT 2 cut(s) 930, 1074
MmeI TCCRAC 1 cut(s) 1103
MnlI CCTC 7 cut(s) 65, 209, 353, 497, 641, 785, 1130
MroXI GAANNNNTTC 1 cut(s) 1043
MseI TTAA 6 cut(s) 41, 185, 329, 473, 617, 761
MslI CAYNNNNRTG 6 cut(s) 57, 201, 345, 489, 633, 777
MspCI CTTAAG 6 cut(s) 40, 184, 328, 472, 616, 760
NcoI CCATGG 6 cut(s) 144, 288, 432, 576, 720, 864
NdeII GATC 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
NlaIII CATG 6 cut(s) 148, 292, 436, 580, 724, 868
OliI CACNNNNGTG 6 cut(s) 57, 201, 345, 489, 633, 777
PdmI GAANNNNTTC 1 cut(s) 1043
PfeI GAWTC 8 cut(s) 31, 175, 319, 463, 607, 751, 895, 1039
PspPI GGNCC 1 cut(s) 998
PsuI RGATCY 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
RseI CAYNNNNRTG 6 cut(s) 57, 201, 345, 489, 633, 777
SaqAI TTAA 6 cut(s) 41, 185, 329, 473, 617, 761
Sau3AI GATC 9 cut(s) 85, 229, 373, 517, 661, 805, 949, 1093, 1220
Sau96I GGNCC 1 cut(s) 998
SduI GDGCHC 1 cut(s) 1055
SmiMI CAYNNNNRTG 6 cut(s) 57, 201, 345, 489, 633, 777
SmlI CTYRAG 8 cut(s) 40, 184, 328, 472, 616, 760, 1048, 1064
SmoI CTYRAG 8 cut(s) 40, 184, 328, 472, 616, 760, 1048, 1064
Sse9I AATT 2 cut(s) 930, 1074
SspMI CTAG 7 cut(s) 80, 224, 368, 512, 656, 800, 1215
StyI CCWWGG 6 cut(s) 144, 288, 432, 576, 720, 864
TaaI ACNGT 1 cut(s) 1191
TasI AATT 2 cut(s) 930, 1074
TfiI GAWTC 8 cut(s) 31, 175, 319, 463, 607, 751, 895, 1039
Tru1I TTAA 6 cut(s) 41, 185, 329, 473, 617, 761
Tru9I TTAA 6 cut(s) 41, 185, 329, 473, 617, 761
TscAI CASTG 9 cut(s) 64, 208, 352, 496, 640, 784, 928, 948, 1092
TspGWI ACGGA 7 cut(s) 60, 204, 348, 492, 636, 780, 1195
TspRI CASTG 9 cut(s) 64, 208, 352, 496, 640, 784, 928, 948, 1092
Vha464I CTTAAG 6 cut(s) 40, 184, 328, 472, 616, 760
XmnI GAANNNNTTC 1 cut(s) 1043
XspI CTAG 7 cut(s) 80, 224, 368, 512, 656, 800, 1215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.